MKS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MKS1
Entrez Gene ID	821400
Full Name	MAP kinase substrate 1
Synonyms	MAP kinase substrate 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_112755.2	NP_566616.1	MAP kinase substrate 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study evaluates the effect of manipulating MAP kinase 4 nuclear substrate 1 (MKS1) expression in Kalanchoe blossfeldiana and Petunia hybrida.
Title: Manipulation of MKS1 gene expression affects Kalancho? blossfeldiana and Petunia hybrida phenotypes.

MKS1 interacts with the transcription factor WRKY33, which in turn controls the regulation of PAD3 and CYP71A13, two genes, required for proper resistance to B. cinerea.
Title: Constitutive expression of MKS1 confers susceptibility to Botrytis cinerea infection independent of PAD3 expression.

MKS1 function and subcellular location requires an intact N-terminus important for both MPK4 and WRKY33 interactions
Title: Arabidopsis MKS1 is involved in basal immunity and requires an intact N-terminal domain for proper function.

three of the twelve Ser-Pro serine residues (Ser72, Ser108, Ser120) are in the phosphorylated form.
Title: Phosphorylation sites of Arabidopsis MAP kinase substrate 1 (MKS1).

MKS1 is required for full salicylic acid-dependent resistance in MAP kinase 4 mutants
Title: The MAP kinase substrate MKS1 is a regulator of plant defense responses.

The following MKS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MKS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb11766	NM_112755.2 Latest version!	Arabidopsis thaliana MAP kinase substrate 1 (MKS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MKS1 ( NM_112755.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_566616.1 Arabidopsis thaliana MAP kinase substrate 1 (MKS1), mRNA.

Price & Availability↑

CloneID	OAb11766
Clone ID Related Accession (Same CDS sequence)	NM_112755.2
Accession Version	NM_112755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	MAP kinase substrate 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Apr 18, 2007 this sequence version replaced NM_112755.1.

ATGGATCCGT CGGAGTATTT TGCCGGCGGC AATCCTTCCG ATCAACAGAA CCAGAAGCGG 
CAGCTTCAGA TCTGTGGTCC TCGTCCTTCA CCTCTTAGTG TTCACAAAGA CTCTCACAAA 
ATCAAGAAAC CTCCAAAACA CCCTGCGCCG CCGCCAAATC GTGACCAACC GCCGCCGTAT 
ATTCCTAGAG AGCCGGTGGT TATCTACGCC GTATCCCCCA AGGTTGTACA CGCAACCGCG 
TCTGAGTTCA TGAACGTAGT CCAGCGACTC ACAGGGATCT CCTCTGGTGT TTTCCTCGAA 
TCTGGCGGCG GTGGAGATGT TTCACCGGCG GCGAGGCTAG CGTCCACGGA AAATGCTAGT 
CCAAGAGGAG GAAAAGAACC GGCTGCGAGA GATGAGACGG TGGAAATCAA CACGGCTATG 
GAAGAAGCAG CTGAATTTGG TGGTTATGCT CCGGGAATAC TCTCGCCATC TCCGGCCTTG 
TTGCCAACAG CTTCTACCGG GATATTCTCT CCGATGTATC ATCAAGGTGG GATGTTTTCG 
CCGGCTATAC CACTGGGATT ATTCTCGCCG GCGGGATTTA TGAGCCCGTT TCGAAGTCCT 
GGCTTTACTA GTTTGGTAGC TTCACCAACT TTTGCTGATT TCTTTAGTCA TATTTGGGAT 
CAAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_566616.1
CDS	92..760
Translation	MDPSEYFAGGNPSDQQNQKRQLQICGPRPSPLSVHKDSHKIKKPPKHPAPPPNRDQPPPYIPREPVVIYAVSPKVVHATASEFMNVVQRLTGISSGVFLESGGGGDVSPAARLASTENASPRGGKEPAARDETVEINTAMEEAAEFGGYAPGILSPSPALLPTASTGIFSPMYHQGGMFSPAIPLGLFSPAGFMSPFRSPGFTSLVASPTFADFFSHIWDQD

Target ORF information:

RefSeq Version	NM_112755.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana MAP kinase substrate 1 (MKS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_112755.2

ORF Insert Sequence:

ATGGATCCGT CGGAGTATTT TGCCGGCGGC AATCCTTCCG ATCAACAGAA CCAGAAGCGG 
CAGCTTCAGA TCTGTGGTCC TCGTCCTTCA CCTCTTAGTG TTCACAAAGA CTCTCACAAA 
ATCAAGAAAC CTCCAAAACA CCCTGCGCCG CCGCCAAATC GTGACCAACC GCCGCCGTAT 
ATTCCTAGAG AGCCGGTGGT TATCTACGCC GTATCCCCCA AGGTTGTACA CGCAACCGCG 
TCTGAGTTCA TGAACGTAGT CCAGCGACTC ACAGGGATCT CCTCTGGTGT TTTCCTCGAA 
TCTGGCGGCG GTGGAGATGT TTCACCGGCG GCGAGGCTAG CGTCCACGGA AAATGCTAGT 
CCAAGAGGAG GAAAAGAACC GGCTGCGAGA GATGAGACGG TGGAAATCAA CACGGCTATG 
GAAGAAGCAG CTGAATTTGG TGGTTATGCT CCGGGAATAC TCTCGCCATC TCCGGCCTTG 
TTGCCAACAG CTTCTACCGG GATATTCTCT CCGATGTATC ATCAAGGTGG GATGTTTTCG 
CCGGCTATAC CACTGGGATT ATTCTCGCCG GCGGGATTTA TGAGCCCGTT TCGAAGTCCT 
GGCTTTACTA GTTTGGTAGC TTCACCAACT TTTGCTGATT TCTTTAGTCA TATTTGGGAT 
CAAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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MKS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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