HMA4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMA4
Entrez Gene ID	816428
Full Name	heavy metal atpase 4
Synonyms	ARABIDOPSIS HEAVY METAL ATPASE 4,ATHMA4,T20K24.12,T20K24_12,heavy metal atpase 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001335621.1	NP_001318247.1	heavy metal atpase 4
NM_001335622.1	NP_001324425.1	heavy metal atpase 4
NM_127468.5	NP_179501.1	heavy metal atpase 4

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	HMA4	NP_179501.1
Arabidopsis thaliana (thale cress)	HMA3	NP_194741.2
Oryza sativa (rice)	Os07g0232900	NP_001059240.2
Arabidopsis thaliana (thale cress)	HMA2	NP_194740.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression modulates the ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco
Title: The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4.

These studies evaluated the the function and structure of the N-terminal cytoplasmic metal-binding domain of HMA4.
Title: Metal binding to the N-terminal cytoplasmic domain of the PIB ATPase HMA4 is required for metal transport in Arabidopsis.

Due to ectopic expression of 35S::AtHMA4, the physical apoplastic barrier within the external cell layer developed, which is likely to be responsible for the reduction of Cd uptake/accumulation.
Title: HMA4 expression in tobacco reduces Cd accumulation due to the induction of the apoplastic barrier.

Ectopically expressing AtHMA4 in tobacco results in overloading the root and leaf apoplast with zinc.
Title: Development of Zn-related necrosis in tobacco is enhanced by expressing AtHMA4 and depends on the apoplastic Zn levels.

the C-terminal domain of AtHMA4 serves a dual role as Zn(2+) and Cd(2+) chelator (sensor) and as a regulator of the efficiency of Zn(2+) and Cd(2+) export.
Title: A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump.

The following HMA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb07363	NM_127468.5 Latest version!	Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb07363	NM_001335621.1 Latest version!	Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb07363	NM_001335622.1 Latest version!	Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMA4 ( NM_001335621.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318247.1 Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.

Price & Availability↑

CloneID	OAb07363
Clone ID Related Accession (Same CDS sequence)	NM_001335621.1 , NM_001335622.1 , NM_127468.5
Accession Version	NM_001335621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heavy metal atpase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGCGTTAC AAAACAAAGA AGAAGAGAAA AAGAAAGTGA AGAAGTTGCA AAAGAGTTAC 
TTCGATGTTC TCGGAATCTG TTGTACATCG GAAGTTCCTA TAATCGAGAA TATTCTCAAG 
TCACTTGACG GCGTTAAAGA ATATTCCGTC ATCGTTCCCT CGAGAACCGT GATTGTTGTT 
CACGACAGTC TCCTCATCTC TCCCTTCCAA ATTGCTAAGG CACTAAACGA AGCTAGGTTA 
GAAGCAAACG TGAGGGTAAA CGGAGAAACT AGCTTCAAGA ACAAATGGCC GAGCCCTTTC 
GCCGTAGTTT CCGGCTTACT TCTCCTCCTA TCCTTCCTAA AGTTTGTCTA CTCGCCTTTA 
CGTTGGCTCG CCGTGGCAGC AGTTGCCGCC GGTATCTATC CGATTCTTGC CAAAGCCTTT 
GCTTCCATTA AAAGGCCTAG GATCGACATC AACATATTGG TCATAATAAC CGTGATTGCA 
ACACTTGCAA TGCAAGATTT CATGGAGGCA GCAGCAGTTG TGTTCCTATT CACCATATCC 
GACTGGCTCG AAACAAGAGC TAGCTACAAG GCGACCTCGG TAATGCAGTC TCTGATGAGC 
TTAGCTCCAC AAAAGGCTAT AATAGCAGAG ACTGGTGAAG AAGTTGAAGT AGATGAGGTT 
AAGGTTGATA CAGTTGTAGC AGTTAAAGCT GGTGAAACCA TACCAATTGA TGGAATTGTG 
GTGGATGGAA ACTGTGAGGT AGACGAGAAA ACCTTAACGG GCGAAGCATT TCCTGTGCCT 
AAACAGAGAG ATTCTACCGT TTGGGCTGGA ACCATTAATC TAAATGGTTA CATATGTGTG 
AAAACAACTT CTTTAGCGGG TGATTGTGTG GTTGCGAAAA TGGCTAAGCT AGTAGAAGAA 
GCTCAGAGCA GTAAAACCAA ATCTCAGAGA CTAATAGACA AATGTTCTCA GTACTATACT 
CCAGCAATCA TCTTAGTATC AGCTTGCGTT GCCATTGTCC CGGTTATAAT GAAGGTCCAC 
AACCTTAAAC ATTGGTTCCA CCTAGCATTA GTTGTGTTAG TCAGTGGTTG TCCCTGTGGT 
CTTATCCTCT CTACACCAGT TGCTACTTTC TGTGCACTTA CTAAAGCGGC AACTTCAGGG 
CTTCTGATCA AAAGTGCTGA TTATCTTGAC ACACTCTCAA AGATCAAGAT TGTTGCTTTC 
GATAAAACTG GGACTATTAC AAGAGGAGAG TTCATTGTCA TAGATTTCAA GTCACTCTCT 
AGAGATATAA ACCTACGCAG CTTGCTTTAC TGGGTATCAA GTGTTGAAAG CAAATCAAGT 
CATCCAATGG CAGCAACAAT CGTGGATTAT GCAAAATCTG TTTCTGTTGA GCCTAGGCCT 
GAAGAGGTTG AGGATTACCA GAACTTTCCA GGTGAAGGAA TCTACGGGAA GATTGATGGT 
AACGATATCT TCATTGGGAA CAAAAAGATA GCTTCTCGAG CTGGTTGTTC AACAGTTCCA 
GAGATTGAAG TTGATACCAA AGGCGGGAAG ACTGTTGGAT ACGTCTATGT AGGTGAAAGA 
CTAGCTGGAT TTTTCAATCT TTCTGATGCT TGTAGATCTG GTGTTTCTCA AGCAATGGCA 
GAACTGAAAT CTCTAGGAAT CAAAACCGCA ATGCTAACGG GAGATAATCA AGCCGCGGCA 
ATGCATGCTC AAGAACAGCT AGGGAATGTT TTAGATGTTG TACATGGAGA TCTTCTTCCA 
GAAGATAAGT CCAGAATCAT ACAAGAGTTT AAGAAAGAGG GACCAACCGC AATGGTAGGG 
GACGGTGTGA ATGATGCACC AGCTTTAGCT ACAGCTGATA TTGGTATCTC CATGGGAATT 
TCTGGCTCTG CTCTTGCAAC ACAAACTGGT AATATTATTC TGATGTCTAA TGATATAAGA 
AGGATACCAC AAGCGGTGAA GCTAGCGAGA AGAGCACGAC GCAAAGTTGT TGAAAACGTG 
TGTCTATCGA TCATTTTAAA AGCAGGAATA CTCGCTTTGG CATTTGCTGG TCATCCTTTG 
ATTTGGGCTG CGGTTCTTGT TGATGTAGGG ACTTGTCTGC TTGTGATTTT CAATAGTATG 
TTGCTGCTGC GAGAGAAGAA AAAGATTGGG AACAAAAAGT GTTACAGGGC TTCTACATCT 
AAGTTGAATG GTAGGAAACT TGAAGGCGAT GATGATTATG TTGTGGACTT AGAAGCAGGC 
TTGTTAACAA AGAGCGGGAA TGGTCAATGC AAATCAAGCT GTTGTGGAGA TAAGAAAAAT 
CAAGAGAATG TTGTGATGAT GAAACCAAGT AGTAAAACCA GTTCTGATCA TTCTCACCCT 
GGTTGTTGTG GCGATAAGAA GGAAGAAAAA GTGAAGCCGC TTGTGAAAGA TGGCTGTTGC 
AGTGAGAAAA CTAGGAAATC AGAGGGAGAT ATGGTTTCAT TGAGCTCATG TAAGAAGTCT 
AGTCATGTCA AACATGACCT GAAAATGAAA GGTGGTTCAG GTTGTTGTGC TAGCAAAAAT 
GAGAAAGGGA AGGAAGTAGT GGCAAAGAGC TGTTGTGAGA AACCCAAACA GCAGGTGGAG 
AGTGTTGGAG ACTGCAAGTC TGGTCATTGC GAGAAGAAGA AGCAAGCTGA AGACATTGTT 
GTCCCGGTGC AGATTATTGG TCATGCATTA ACGCATGTGG AGATCGAGTT GCAGACAAAG 
GAAACCTGCA AAACAAGCTG TTGTGACAGT AAAGAAAAGG TTAAGGAGAC AGGTTTGCTG 
CTTTCTAGTG AGAACACACC TTACCTGGAG AAAGGAGTGC TGATTAAAGA TGAAGGAAAC 
TGCAAGTCTG GCAGCGAGAA CATGGGGACA GTGAAACAAA GCTGCCATGA GAAGGGCTGC 
AGCGATGAAA AACAAACCGG GGAAATAACT CTTGCTTCGG AGGAAGAGAC AGATGATCAA 
GATTGCTCCT CGGGATGTTG TGTGAACGAG GGAACAGTGA AACAAAGCTT CGATGAGAAG 
AAGCATTCTG TGTTGGTGGA GAAGGAAGGT TTGGACATGG AAACTGGTTT CTGTTGTGAT 
GCCAAGCTGG TTTGTTGTGG AAACACAGAA GGTGAAGTGA AGGAGCAATG TCGTCTGGAG 
ATAAAGAAAG AAGAACATTG CAAGTCTGGT TGCTGCGGCG AGGAAATACA AACCGGAGAA 
ATCACTCTGG TTTCAGAGGA AGAGACAGAG AGCACGAATT GTTCCACGGG TTGTTGTGTG 
GACAAAGAAG AAGTGACACA AACCTGTCAT GAGAAGCCTG CTAGCTTGGT GGTATCAGGC 
TTGGAAGTGA AGAAGGATGA GCATTGTGAG AGCTCACACA GAGCCGTCAA GGTAGAGACC 
TGTTGCAAAG TGAAGATTCC AGAGGCTTGC GCATCAAAAT GTAGGGACAG AGCGAAGCGT 
CACAGTGGTA AAAGCTGTTG CAGGAGTTAT GCAAAAGAGT TATGCAGCCA CCGCCATCAT 
CATCACCACC ACCACCACCA TCACCATGTG AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318247.1
CDS	42..3560
Translation	MALQNKEEEKKKVKKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLLISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLETRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRASTSKLNGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSHPGCCGDKKEEKVKPLVKDGCCSEKTRKSEGDMVSLSSCKKSSHVKHDLKMKGGSGCCASKNEKGKEVVAKSCCEKPKQQVESVGDCKSGHCEKKKQAEDIVVPVQIIGHALTHVEIELQTKETCKTSCCDSKEKVKETGLLLSSENTPYLEKGVLIKDEGNCKSGSENMGTVKQSCHEKGCSDEKQTGEITLASEEETDDQDCSSGCCVNEGTVKQSFDEKKHSVLVEKEGLDMETGFCCDAKLVCCGNTEGEVKEQCRLEIKKEEHCKSGCCGEEIQTGEITLVSEEETESTNCSTGCCVDKEEVTQTCHEKPASLVVSGLEVKKDEHCESSHRAVKVETCCKVKIPEACASKCRDRAKRHSGKSCCRSYAKELCSHRHHHHHHHHHHHVSA

Target ORF information:

RefSeq Version	NM_001335621.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001335621.1

ORF Insert Sequence:

ATGGCGTTAC AAAACAAAGA AGAAGAGAAA AAGAAAGTGA AGAAGTTGCA AAAGAGTTAC 
TTCGATGTTC TCGGAATCTG TTGTACATCG GAAGTTCCTA TAATCGAGAA TATTCTCAAG 
TCACTTGACG GCGTTAAAGA ATATTCCGTC ATCGTTCCCT CGAGAACCGT GATTGTTGTT 
CACGACAGTC TCCTCATCTC TCCCTTCCAA ATTGCTAAGG CACTAAACGA AGCTAGGTTA 
GAAGCAAACG TGAGGGTAAA CGGAGAAACT AGCTTCAAGA ACAAATGGCC GAGCCCTTTC 
GCCGTAGTTT CCGGCTTACT TCTCCTCCTA TCCTTCCTAA AGTTTGTCTA CTCGCCTTTA 
CGTTGGCTCG CCGTGGCAGC AGTTGCCGCC GGTATCTATC CGATTCTTGC CAAAGCCTTT 
GCTTCCATTA AAAGGCCTAG GATCGACATC AACATATTGG TCATAATAAC CGTGATTGCA 
ACACTTGCAA TGCAAGATTT CATGGAGGCA GCAGCAGTTG TGTTCCTATT CACCATATCC 
GACTGGCTCG AAACAAGAGC TAGCTACAAG GCGACCTCGG TAATGCAGTC TCTGATGAGC 
TTAGCTCCAC AAAAGGCTAT AATAGCAGAG ACTGGTGAAG AAGTTGAAGT AGATGAGGTT 
AAGGTTGATA CAGTTGTAGC AGTTAAAGCT GGTGAAACCA TACCAATTGA TGGAATTGTG 
GTGGATGGAA ACTGTGAGGT AGACGAGAAA ACCTTAACGG GCGAAGCATT TCCTGTGCCT 
AAACAGAGAG ATTCTACCGT TTGGGCTGGA ACCATTAATC TAAATGGTTA CATATGTGTG 
AAAACAACTT CTTTAGCGGG TGATTGTGTG GTTGCGAAAA TGGCTAAGCT AGTAGAAGAA 
GCTCAGAGCA GTAAAACCAA ATCTCAGAGA CTAATAGACA AATGTTCTCA GTACTATACT 
CCAGCAATCA TCTTAGTATC AGCTTGCGTT GCCATTGTCC CGGTTATAAT GAAGGTCCAC 
AACCTTAAAC ATTGGTTCCA CCTAGCATTA GTTGTGTTAG TCAGTGGTTG TCCCTGTGGT 
CTTATCCTCT CTACACCAGT TGCTACTTTC TGTGCACTTA CTAAAGCGGC AACTTCAGGG 
CTTCTGATCA AAAGTGCTGA TTATCTTGAC ACACTCTCAA AGATCAAGAT TGTTGCTTTC 
GATAAAACTG GGACTATTAC AAGAGGAGAG TTCATTGTCA TAGATTTCAA GTCACTCTCT 
AGAGATATAA ACCTACGCAG CTTGCTTTAC TGGGTATCAA GTGTTGAAAG CAAATCAAGT 
CATCCAATGG CAGCAACAAT CGTGGATTAT GCAAAATCTG TTTCTGTTGA GCCTAGGCCT 
GAAGAGGTTG AGGATTACCA GAACTTTCCA GGTGAAGGAA TCTACGGGAA GATTGATGGT 
AACGATATCT TCATTGGGAA CAAAAAGATA GCTTCTCGAG CTGGTTGTTC AACAGTTCCA 
GAGATTGAAG TTGATACCAA AGGCGGGAAG ACTGTTGGAT ACGTCTATGT AGGTGAAAGA 
CTAGCTGGAT TTTTCAATCT TTCTGATGCT TGTAGATCTG GTGTTTCTCA AGCAATGGCA 
GAACTGAAAT CTCTAGGAAT CAAAACCGCA ATGCTAACGG GAGATAATCA AGCCGCGGCA 
ATGCATGCTC AAGAACAGCT AGGGAATGTT TTAGATGTTG TACATGGAGA TCTTCTTCCA 
GAAGATAAGT CCAGAATCAT ACAAGAGTTT AAGAAAGAGG GACCAACCGC AATGGTAGGG 
GACGGTGTGA ATGATGCACC AGCTTTAGCT ACAGCTGATA TTGGTATCTC CATGGGAATT 
TCTGGCTCTG CTCTTGCAAC ACAAACTGGT AATATTATTC TGATGTCTAA TGATATAAGA 
AGGATACCAC AAGCGGTGAA GCTAGCGAGA AGAGCACGAC GCAAAGTTGT TGAAAACGTG 
TGTCTATCGA TCATTTTAAA AGCAGGAATA CTCGCTTTGG CATTTGCTGG TCATCCTTTG 
ATTTGGGCTG CGGTTCTTGT TGATGTAGGG ACTTGTCTGC TTGTGATTTT CAATAGTATG 
TTGCTGCTGC GAGAGAAGAA AAAGATTGGG AACAAAAAGT GTTACAGGGC TTCTACATCT 
AAGTTGAATG GTAGGAAACT TGAAGGCGAT GATGATTATG TTGTGGACTT AGAAGCAGGC 
TTGTTAACAA AGAGCGGGAA TGGTCAATGC AAATCAAGCT GTTGTGGAGA TAAGAAAAAT 
CAAGAGAATG TTGTGATGAT GAAACCAAGT AGTAAAACCA GTTCTGATCA TTCTCACCCT 
GGTTGTTGTG GCGATAAGAA GGAAGAAAAA GTGAAGCCGC TTGTGAAAGA TGGCTGTTGC 
AGTGAGAAAA CTAGGAAATC AGAGGGAGAT ATGGTTTCAT TGAGCTCATG TAAGAAGTCT 
AGTCATGTCA AACATGACCT GAAAATGAAA GGTGGTTCAG GTTGTTGTGC TAGCAAAAAT 
GAGAAAGGGA AGGAAGTAGT GGCAAAGAGC TGTTGTGAGA AACCCAAACA GCAGGTGGAG 
AGTGTTGGAG ACTGCAAGTC TGGTCATTGC GAGAAGAAGA AGCAAGCTGA AGACATTGTT 
GTCCCGGTGC AGATTATTGG TCATGCATTA ACGCATGTGG AGATCGAGTT GCAGACAAAG 
GAAACCTGCA AAACAAGCTG TTGTGACAGT AAAGAAAAGG TTAAGGAGAC AGGTTTGCTG 
CTTTCTAGTG AGAACACACC TTACCTGGAG AAAGGAGTGC TGATTAAAGA TGAAGGAAAC 
TGCAAGTCTG GCAGCGAGAA CATGGGGACA GTGAAACAAA GCTGCCATGA GAAGGGCTGC 
AGCGATGAAA AACAAACCGG GGAAATAACT CTTGCTTCGG AGGAAGAGAC AGATGATCAA 
GATTGCTCCT CGGGATGTTG TGTGAACGAG GGAACAGTGA AACAAAGCTT CGATGAGAAG 
AAGCATTCTG TGTTGGTGGA GAAGGAAGGT TTGGACATGG AAACTGGTTT CTGTTGTGAT 
GCCAAGCTGG TTTGTTGTGG AAACACAGAA GGTGAAGTGA AGGAGCAATG TCGTCTGGAG 
ATAAAGAAAG AAGAACATTG CAAGTCTGGT TGCTGCGGCG AGGAAATACA AACCGGAGAA 
ATCACTCTGG TTTCAGAGGA AGAGACAGAG AGCACGAATT GTTCCACGGG TTGTTGTGTG 
GACAAAGAAG AAGTGACACA AACCTGTCAT GAGAAGCCTG CTAGCTTGGT GGTATCAGGC 
TTGGAAGTGA AGAAGGATGA GCATTGTGAG AGCTCACACA GAGCCGTCAA GGTAGAGACC 
TGTTGCAAAG TGAAGATTCC AGAGGCTTGC GCATCAAAAT GTAGGGACAG AGCGAAGCGT 
CACAGTGGTA AAAGCTGTTG CAGGAGTTAT GCAAAAGAGT TATGCAGCCA CCGCCATCAT 
CATCACCACC ACCACCACCA TCACCATGTG AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMA4 ( NM_001335622.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324425.1 Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.

Price & Availability↑

CloneID	OAb07363
Clone ID Related Accession (Same CDS sequence)	NM_001335621.1 , NM_001335622.1 , NM_127468.5
Accession Version	NM_001335622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heavy metal atpase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGCGTTAC AAAACAAAGA AGAAGAGAAA AAGAAAGTGA AGAAGTTGCA AAAGAGTTAC 
TTCGATGTTC TCGGAATCTG TTGTACATCG GAAGTTCCTA TAATCGAGAA TATTCTCAAG 
TCACTTGACG GCGTTAAAGA ATATTCCGTC ATCGTTCCCT CGAGAACCGT GATTGTTGTT 
CACGACAGTC TCCTCATCTC TCCCTTCCAA ATTGCTAAGG CACTAAACGA AGCTAGGTTA 
GAAGCAAACG TGAGGGTAAA CGGAGAAACT AGCTTCAAGA ACAAATGGCC GAGCCCTTTC 
GCCGTAGTTT CCGGCTTACT TCTCCTCCTA TCCTTCCTAA AGTTTGTCTA CTCGCCTTTA 
CGTTGGCTCG CCGTGGCAGC AGTTGCCGCC GGTATCTATC CGATTCTTGC CAAAGCCTTT 
GCTTCCATTA AAAGGCCTAG GATCGACATC AACATATTGG TCATAATAAC CGTGATTGCA 
ACACTTGCAA TGCAAGATTT CATGGAGGCA GCAGCAGTTG TGTTCCTATT CACCATATCC 
GACTGGCTCG AAACAAGAGC TAGCTACAAG GCGACCTCGG TAATGCAGTC TCTGATGAGC 
TTAGCTCCAC AAAAGGCTAT AATAGCAGAG ACTGGTGAAG AAGTTGAAGT AGATGAGGTT 
AAGGTTGATA CAGTTGTAGC AGTTAAAGCT GGTGAAACCA TACCAATTGA TGGAATTGTG 
GTGGATGGAA ACTGTGAGGT AGACGAGAAA ACCTTAACGG GCGAAGCATT TCCTGTGCCT 
AAACAGAGAG ATTCTACCGT TTGGGCTGGA ACCATTAATC TAAATGGTTA CATATGTGTG 
AAAACAACTT CTTTAGCGGG TGATTGTGTG GTTGCGAAAA TGGCTAAGCT AGTAGAAGAA 
GCTCAGAGCA GTAAAACCAA ATCTCAGAGA CTAATAGACA AATGTTCTCA GTACTATACT 
CCAGCAATCA TCTTAGTATC AGCTTGCGTT GCCATTGTCC CGGTTATAAT GAAGGTCCAC 
AACCTTAAAC ATTGGTTCCA CCTAGCATTA GTTGTGTTAG TCAGTGGTTG TCCCTGTGGT 
CTTATCCTCT CTACACCAGT TGCTACTTTC TGTGCACTTA CTAAAGCGGC AACTTCAGGG 
CTTCTGATCA AAAGTGCTGA TTATCTTGAC ACACTCTCAA AGATCAAGAT TGTTGCTTTC 
GATAAAACTG GGACTATTAC AAGAGGAGAG TTCATTGTCA TAGATTTCAA GTCACTCTCT 
AGAGATATAA ACCTACGCAG CTTGCTTTAC TGGGTATCAA GTGTTGAAAG CAAATCAAGT 
CATCCAATGG CAGCAACAAT CGTGGATTAT GCAAAATCTG TTTCTGTTGA GCCTAGGCCT 
GAAGAGGTTG AGGATTACCA GAACTTTCCA GGTGAAGGAA TCTACGGGAA GATTGATGGT 
AACGATATCT TCATTGGGAA CAAAAAGATA GCTTCTCGAG CTGGTTGTTC AACAGTTCCA 
GAGATTGAAG TTGATACCAA AGGCGGGAAG ACTGTTGGAT ACGTCTATGT AGGTGAAAGA 
CTAGCTGGAT TTTTCAATCT TTCTGATGCT TGTAGATCTG GTGTTTCTCA AGCAATGGCA 
GAACTGAAAT CTCTAGGAAT CAAAACCGCA ATGCTAACGG GAGATAATCA AGCCGCGGCA 
ATGCATGCTC AAGAACAGCT AGGGAATGTT TTAGATGTTG TACATGGAGA TCTTCTTCCA 
GAAGATAAGT CCAGAATCAT ACAAGAGTTT AAGAAAGAGG GACCAACCGC AATGGTAGGG 
GACGGTGTGA ATGATGCACC AGCTTTAGCT ACAGCTGATA TTGGTATCTC CATGGGAATT 
TCTGGCTCTG CTCTTGCAAC ACAAACTGGT AATATTATTC TGATGTCTAA TGATATAAGA 
AGGATACCAC AAGCGGTGAA GCTAGCGAGA AGAGCACGAC GCAAAGTTGT TGAAAACGTG 
TGTCTATCGA TCATTTTAAA AGCAGGAATA CTCGCTTTGG CATTTGCTGG TCATCCTTTG 
ATTTGGGCTG CGGTTCTTGT TGATGTAGGG ACTTGTCTGC TTGTGATTTT CAATAGTATG 
TTGCTGCTGC GAGAGAAGAA AAAGATTGGG AACAAAAAGT GTTACAGGGC TTCTACATCT 
AAGTTGAATG GTAGGAAACT TGAAGGCGAT GATGATTATG TTGTGGACTT AGAAGCAGGC 
TTGTTAACAA AGAGCGGGAA TGGTCAATGC AAATCAAGCT GTTGTGGAGA TAAGAAAAAT 
CAAGAGAATG TTGTGATGAT GAAACCAAGT AGTAAAACCA GTTCTGATCA TTCTCACCCT 
GGTTGTTGTG GCGATAAGAA GGAAGAAAAA GTGAAGCCGC TTGTGAAAGA TGGCTGTTGC 
AGTGAGAAAA CTAGGAAATC AGAGGGAGAT ATGGTTTCAT TGAGCTCATG TAAGAAGTCT 
AGTCATGTCA AACATGACCT GAAAATGAAA GGTGGTTCAG GTTGTTGTGC TAGCAAAAAT 
GAGAAAGGGA AGGAAGTAGT GGCAAAGAGC TGTTGTGAGA AACCCAAACA GCAGGTGGAG 
AGTGTTGGAG ACTGCAAGTC TGGTCATTGC GAGAAGAAGA AGCAAGCTGA AGACATTGTT 
GTCCCGGTGC AGATTATTGG TCATGCATTA ACGCATGTGG AGATCGAGTT GCAGACAAAG 
GAAACCTGCA AAACAAGCTG TTGTGACAGT AAAGAAAAGG TTAAGGAGAC AGGTTTGCTG 
CTTTCTAGTG AGAACACACC TTACCTGGAG AAAGGAGTGC TGATTAAAGA TGAAGGAAAC 
TGCAAGTCTG GCAGCGAGAA CATGGGGACA GTGAAACAAA GCTGCCATGA GAAGGGCTGC 
AGCGATGAAA AACAAACCGG GGAAATAACT CTTGCTTCGG AGGAAGAGAC AGATGATCAA 
GATTGCTCCT CGGGATGTTG TGTGAACGAG GGAACAGTGA AACAAAGCTT CGATGAGAAG 
AAGCATTCTG TGTTGGTGGA GAAGGAAGGT TTGGACATGG AAACTGGTTT CTGTTGTGAT 
GCCAAGCTGG TTTGTTGTGG AAACACAGAA GGTGAAGTGA AGGAGCAATG TCGTCTGGAG 
ATAAAGAAAG AAGAACATTG CAAGTCTGGT TGCTGCGGCG AGGAAATACA AACCGGAGAA 
ATCACTCTGG TTTCAGAGGA AGAGACAGAG AGCACGAATT GTTCCACGGG TTGTTGTGTG 
GACAAAGAAG AAGTGACACA AACCTGTCAT GAGAAGCCTG CTAGCTTGGT GGTATCAGGC 
TTGGAAGTGA AGAAGGATGA GCATTGTGAG AGCTCACACA GAGCCGTCAA GGTAGAGACC 
TGTTGCAAAG TGAAGATTCC AGAGGCTTGC GCATCAAAAT GTAGGGACAG AGCGAAGCGT 
CACAGTGGTA AAAGCTGTTG CAGGAGTTAT GCAAAAGAGT TATGCAGCCA CCGCCATCAT 
CATCACCACC ACCACCACCA TCACCATGTG AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324425.1
CDS	394..3912
Translation	MALQNKEEEKKKVKKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLLISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLETRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRASTSKLNGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSHPGCCGDKKEEKVKPLVKDGCCSEKTRKSEGDMVSLSSCKKSSHVKHDLKMKGGSGCCASKNEKGKEVVAKSCCEKPKQQVESVGDCKSGHCEKKKQAEDIVVPVQIIGHALTHVEIELQTKETCKTSCCDSKEKVKETGLLLSSENTPYLEKGVLIKDEGNCKSGSENMGTVKQSCHEKGCSDEKQTGEITLASEEETDDQDCSSGCCVNEGTVKQSFDEKKHSVLVEKEGLDMETGFCCDAKLVCCGNTEGEVKEQCRLEIKKEEHCKSGCCGEEIQTGEITLVSEEETESTNCSTGCCVDKEEVTQTCHEKPASLVVSGLEVKKDEHCESSHRAVKVETCCKVKIPEACASKCRDRAKRHSGKSCCRSYAKELCSHRHHHHHHHHHHHVSA

Target ORF information:

RefSeq Version	NM_001335622.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001335622.1

ORF Insert Sequence:

ATGGCGTTAC AAAACAAAGA AGAAGAGAAA AAGAAAGTGA AGAAGTTGCA AAAGAGTTAC 
TTCGATGTTC TCGGAATCTG TTGTACATCG GAAGTTCCTA TAATCGAGAA TATTCTCAAG 
TCACTTGACG GCGTTAAAGA ATATTCCGTC ATCGTTCCCT CGAGAACCGT GATTGTTGTT 
CACGACAGTC TCCTCATCTC TCCCTTCCAA ATTGCTAAGG CACTAAACGA AGCTAGGTTA 
GAAGCAAACG TGAGGGTAAA CGGAGAAACT AGCTTCAAGA ACAAATGGCC GAGCCCTTTC 
GCCGTAGTTT CCGGCTTACT TCTCCTCCTA TCCTTCCTAA AGTTTGTCTA CTCGCCTTTA 
CGTTGGCTCG CCGTGGCAGC AGTTGCCGCC GGTATCTATC CGATTCTTGC CAAAGCCTTT 
GCTTCCATTA AAAGGCCTAG GATCGACATC AACATATTGG TCATAATAAC CGTGATTGCA 
ACACTTGCAA TGCAAGATTT CATGGAGGCA GCAGCAGTTG TGTTCCTATT CACCATATCC 
GACTGGCTCG AAACAAGAGC TAGCTACAAG GCGACCTCGG TAATGCAGTC TCTGATGAGC 
TTAGCTCCAC AAAAGGCTAT AATAGCAGAG ACTGGTGAAG AAGTTGAAGT AGATGAGGTT 
AAGGTTGATA CAGTTGTAGC AGTTAAAGCT GGTGAAACCA TACCAATTGA TGGAATTGTG 
GTGGATGGAA ACTGTGAGGT AGACGAGAAA ACCTTAACGG GCGAAGCATT TCCTGTGCCT 
AAACAGAGAG ATTCTACCGT TTGGGCTGGA ACCATTAATC TAAATGGTTA CATATGTGTG 
AAAACAACTT CTTTAGCGGG TGATTGTGTG GTTGCGAAAA TGGCTAAGCT AGTAGAAGAA 
GCTCAGAGCA GTAAAACCAA ATCTCAGAGA CTAATAGACA AATGTTCTCA GTACTATACT 
CCAGCAATCA TCTTAGTATC AGCTTGCGTT GCCATTGTCC CGGTTATAAT GAAGGTCCAC 
AACCTTAAAC ATTGGTTCCA CCTAGCATTA GTTGTGTTAG TCAGTGGTTG TCCCTGTGGT 
CTTATCCTCT CTACACCAGT TGCTACTTTC TGTGCACTTA CTAAAGCGGC AACTTCAGGG 
CTTCTGATCA AAAGTGCTGA TTATCTTGAC ACACTCTCAA AGATCAAGAT TGTTGCTTTC 
GATAAAACTG GGACTATTAC AAGAGGAGAG TTCATTGTCA TAGATTTCAA GTCACTCTCT 
AGAGATATAA ACCTACGCAG CTTGCTTTAC TGGGTATCAA GTGTTGAAAG CAAATCAAGT 
CATCCAATGG CAGCAACAAT CGTGGATTAT GCAAAATCTG TTTCTGTTGA GCCTAGGCCT 
GAAGAGGTTG AGGATTACCA GAACTTTCCA GGTGAAGGAA TCTACGGGAA GATTGATGGT 
AACGATATCT TCATTGGGAA CAAAAAGATA GCTTCTCGAG CTGGTTGTTC AACAGTTCCA 
GAGATTGAAG TTGATACCAA AGGCGGGAAG ACTGTTGGAT ACGTCTATGT AGGTGAAAGA 
CTAGCTGGAT TTTTCAATCT TTCTGATGCT TGTAGATCTG GTGTTTCTCA AGCAATGGCA 
GAACTGAAAT CTCTAGGAAT CAAAACCGCA ATGCTAACGG GAGATAATCA AGCCGCGGCA 
ATGCATGCTC AAGAACAGCT AGGGAATGTT TTAGATGTTG TACATGGAGA TCTTCTTCCA 
GAAGATAAGT CCAGAATCAT ACAAGAGTTT AAGAAAGAGG GACCAACCGC AATGGTAGGG 
GACGGTGTGA ATGATGCACC AGCTTTAGCT ACAGCTGATA TTGGTATCTC CATGGGAATT 
TCTGGCTCTG CTCTTGCAAC ACAAACTGGT AATATTATTC TGATGTCTAA TGATATAAGA 
AGGATACCAC AAGCGGTGAA GCTAGCGAGA AGAGCACGAC GCAAAGTTGT TGAAAACGTG 
TGTCTATCGA TCATTTTAAA AGCAGGAATA CTCGCTTTGG CATTTGCTGG TCATCCTTTG 
ATTTGGGCTG CGGTTCTTGT TGATGTAGGG ACTTGTCTGC TTGTGATTTT CAATAGTATG 
TTGCTGCTGC GAGAGAAGAA AAAGATTGGG AACAAAAAGT GTTACAGGGC TTCTACATCT 
AAGTTGAATG GTAGGAAACT TGAAGGCGAT GATGATTATG TTGTGGACTT AGAAGCAGGC 
TTGTTAACAA AGAGCGGGAA TGGTCAATGC AAATCAAGCT GTTGTGGAGA TAAGAAAAAT 
CAAGAGAATG TTGTGATGAT GAAACCAAGT AGTAAAACCA GTTCTGATCA TTCTCACCCT 
GGTTGTTGTG GCGATAAGAA GGAAGAAAAA GTGAAGCCGC TTGTGAAAGA TGGCTGTTGC 
AGTGAGAAAA CTAGGAAATC AGAGGGAGAT ATGGTTTCAT TGAGCTCATG TAAGAAGTCT 
AGTCATGTCA AACATGACCT GAAAATGAAA GGTGGTTCAG GTTGTTGTGC TAGCAAAAAT 
GAGAAAGGGA AGGAAGTAGT GGCAAAGAGC TGTTGTGAGA AACCCAAACA GCAGGTGGAG 
AGTGTTGGAG ACTGCAAGTC TGGTCATTGC GAGAAGAAGA AGCAAGCTGA AGACATTGTT 
GTCCCGGTGC AGATTATTGG TCATGCATTA ACGCATGTGG AGATCGAGTT GCAGACAAAG 
GAAACCTGCA AAACAAGCTG TTGTGACAGT AAAGAAAAGG TTAAGGAGAC AGGTTTGCTG 
CTTTCTAGTG AGAACACACC TTACCTGGAG AAAGGAGTGC TGATTAAAGA TGAAGGAAAC 
TGCAAGTCTG GCAGCGAGAA CATGGGGACA GTGAAACAAA GCTGCCATGA GAAGGGCTGC 
AGCGATGAAA AACAAACCGG GGAAATAACT CTTGCTTCGG AGGAAGAGAC AGATGATCAA 
GATTGCTCCT CGGGATGTTG TGTGAACGAG GGAACAGTGA AACAAAGCTT CGATGAGAAG 
AAGCATTCTG TGTTGGTGGA GAAGGAAGGT TTGGACATGG AAACTGGTTT CTGTTGTGAT 
GCCAAGCTGG TTTGTTGTGG AAACACAGAA GGTGAAGTGA AGGAGCAATG TCGTCTGGAG 
ATAAAGAAAG AAGAACATTG CAAGTCTGGT TGCTGCGGCG AGGAAATACA AACCGGAGAA 
ATCACTCTGG TTTCAGAGGA AGAGACAGAG AGCACGAATT GTTCCACGGG TTGTTGTGTG 
GACAAAGAAG AAGTGACACA AACCTGTCAT GAGAAGCCTG CTAGCTTGGT GGTATCAGGC 
TTGGAAGTGA AGAAGGATGA GCATTGTGAG AGCTCACACA GAGCCGTCAA GGTAGAGACC 
TGTTGCAAAG TGAAGATTCC AGAGGCTTGC GCATCAAAAT GTAGGGACAG AGCGAAGCGT 
CACAGTGGTA AAAGCTGTTG CAGGAGTTAT GCAAAAGAGT TATGCAGCCA CCGCCATCAT 
CATCACCACC ACCACCACCA TCACCATGTG AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMA4 ( NM_127468.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179501.1 Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.

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CloneID	OAb07363
Clone ID Related Accession (Same CDS sequence)	NM_001335621.1 , NM_001335622.1 , NM_127468.5
Accession Version	NM_127468.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heavy metal atpase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127468.4.

ATGGCGTTAC AAAACAAAGA AGAAGAGAAA AAGAAAGTGA AGAAGTTGCA AAAGAGTTAC 
TTCGATGTTC TCGGAATCTG TTGTACATCG GAAGTTCCTA TAATCGAGAA TATTCTCAAG 
TCACTTGACG GCGTTAAAGA ATATTCCGTC ATCGTTCCCT CGAGAACCGT GATTGTTGTT 
CACGACAGTC TCCTCATCTC TCCCTTCCAA ATTGCTAAGG CACTAAACGA AGCTAGGTTA 
GAAGCAAACG TGAGGGTAAA CGGAGAAACT AGCTTCAAGA ACAAATGGCC GAGCCCTTTC 
GCCGTAGTTT CCGGCTTACT TCTCCTCCTA TCCTTCCTAA AGTTTGTCTA CTCGCCTTTA 
CGTTGGCTCG CCGTGGCAGC AGTTGCCGCC GGTATCTATC CGATTCTTGC CAAAGCCTTT 
GCTTCCATTA AAAGGCCTAG GATCGACATC AACATATTGG TCATAATAAC CGTGATTGCA 
ACACTTGCAA TGCAAGATTT CATGGAGGCA GCAGCAGTTG TGTTCCTATT CACCATATCC 
GACTGGCTCG AAACAAGAGC TAGCTACAAG GCGACCTCGG TAATGCAGTC TCTGATGAGC 
TTAGCTCCAC AAAAGGCTAT AATAGCAGAG ACTGGTGAAG AAGTTGAAGT AGATGAGGTT 
AAGGTTGATA CAGTTGTAGC AGTTAAAGCT GGTGAAACCA TACCAATTGA TGGAATTGTG 
GTGGATGGAA ACTGTGAGGT AGACGAGAAA ACCTTAACGG GCGAAGCATT TCCTGTGCCT 
AAACAGAGAG ATTCTACCGT TTGGGCTGGA ACCATTAATC TAAATGGTTA CATATGTGTG 
AAAACAACTT CTTTAGCGGG TGATTGTGTG GTTGCGAAAA TGGCTAAGCT AGTAGAAGAA 
GCTCAGAGCA GTAAAACCAA ATCTCAGAGA CTAATAGACA AATGTTCTCA GTACTATACT 
CCAGCAATCA TCTTAGTATC AGCTTGCGTT GCCATTGTCC CGGTTATAAT GAAGGTCCAC 
AACCTTAAAC ATTGGTTCCA CCTAGCATTA GTTGTGTTAG TCAGTGGTTG TCCCTGTGGT 
CTTATCCTCT CTACACCAGT TGCTACTTTC TGTGCACTTA CTAAAGCGGC AACTTCAGGG 
CTTCTGATCA AAAGTGCTGA TTATCTTGAC ACACTCTCAA AGATCAAGAT TGTTGCTTTC 
GATAAAACTG GGACTATTAC AAGAGGAGAG TTCATTGTCA TAGATTTCAA GTCACTCTCT 
AGAGATATAA ACCTACGCAG CTTGCTTTAC TGGGTATCAA GTGTTGAAAG CAAATCAAGT 
CATCCAATGG CAGCAACAAT CGTGGATTAT GCAAAATCTG TTTCTGTTGA GCCTAGGCCT 
GAAGAGGTTG AGGATTACCA GAACTTTCCA GGTGAAGGAA TCTACGGGAA GATTGATGGT 
AACGATATCT TCATTGGGAA CAAAAAGATA GCTTCTCGAG CTGGTTGTTC AACAGTTCCA 
GAGATTGAAG TTGATACCAA AGGCGGGAAG ACTGTTGGAT ACGTCTATGT AGGTGAAAGA 
CTAGCTGGAT TTTTCAATCT TTCTGATGCT TGTAGATCTG GTGTTTCTCA AGCAATGGCA 
GAACTGAAAT CTCTAGGAAT CAAAACCGCA ATGCTAACGG GAGATAATCA AGCCGCGGCA 
ATGCATGCTC AAGAACAGCT AGGGAATGTT TTAGATGTTG TACATGGAGA TCTTCTTCCA 
GAAGATAAGT CCAGAATCAT ACAAGAGTTT AAGAAAGAGG GACCAACCGC AATGGTAGGG 
GACGGTGTGA ATGATGCACC AGCTTTAGCT ACAGCTGATA TTGGTATCTC CATGGGAATT 
TCTGGCTCTG CTCTTGCAAC ACAAACTGGT AATATTATTC TGATGTCTAA TGATATAAGA 
AGGATACCAC AAGCGGTGAA GCTAGCGAGA AGAGCACGAC GCAAAGTTGT TGAAAACGTG 
TGTCTATCGA TCATTTTAAA AGCAGGAATA CTCGCTTTGG CATTTGCTGG TCATCCTTTG 
ATTTGGGCTG CGGTTCTTGT TGATGTAGGG ACTTGTCTGC TTGTGATTTT CAATAGTATG 
TTGCTGCTGC GAGAGAAGAA AAAGATTGGG AACAAAAAGT GTTACAGGGC TTCTACATCT 
AAGTTGAATG GTAGGAAACT TGAAGGCGAT GATGATTATG TTGTGGACTT AGAAGCAGGC 
TTGTTAACAA AGAGCGGGAA TGGTCAATGC AAATCAAGCT GTTGTGGAGA TAAGAAAAAT 
CAAGAGAATG TTGTGATGAT GAAACCAAGT AGTAAAACCA GTTCTGATCA TTCTCACCCT 
GGTTGTTGTG GCGATAAGAA GGAAGAAAAA GTGAAGCCGC TTGTGAAAGA TGGCTGTTGC 
AGTGAGAAAA CTAGGAAATC AGAGGGAGAT ATGGTTTCAT TGAGCTCATG TAAGAAGTCT 
AGTCATGTCA AACATGACCT GAAAATGAAA GGTGGTTCAG GTTGTTGTGC TAGCAAAAAT 
GAGAAAGGGA AGGAAGTAGT GGCAAAGAGC TGTTGTGAGA AACCCAAACA GCAGGTGGAG 
AGTGTTGGAG ACTGCAAGTC TGGTCATTGC GAGAAGAAGA AGCAAGCTGA AGACATTGTT 
GTCCCGGTGC AGATTATTGG TCATGCATTA ACGCATGTGG AGATCGAGTT GCAGACAAAG 
GAAACCTGCA AAACAAGCTG TTGTGACAGT AAAGAAAAGG TTAAGGAGAC AGGTTTGCTG 
CTTTCTAGTG AGAACACACC TTACCTGGAG AAAGGAGTGC TGATTAAAGA TGAAGGAAAC 
TGCAAGTCTG GCAGCGAGAA CATGGGGACA GTGAAACAAA GCTGCCATGA GAAGGGCTGC 
AGCGATGAAA AACAAACCGG GGAAATAACT CTTGCTTCGG AGGAAGAGAC AGATGATCAA 
GATTGCTCCT CGGGATGTTG TGTGAACGAG GGAACAGTGA AACAAAGCTT CGATGAGAAG 
AAGCATTCTG TGTTGGTGGA GAAGGAAGGT TTGGACATGG AAACTGGTTT CTGTTGTGAT 
GCCAAGCTGG TTTGTTGTGG AAACACAGAA GGTGAAGTGA AGGAGCAATG TCGTCTGGAG 
ATAAAGAAAG AAGAACATTG CAAGTCTGGT TGCTGCGGCG AGGAAATACA AACCGGAGAA 
ATCACTCTGG TTTCAGAGGA AGAGACAGAG AGCACGAATT GTTCCACGGG TTGTTGTGTG 
GACAAAGAAG AAGTGACACA AACCTGTCAT GAGAAGCCTG CTAGCTTGGT GGTATCAGGC 
TTGGAAGTGA AGAAGGATGA GCATTGTGAG AGCTCACACA GAGCCGTCAA GGTAGAGACC 
TGTTGCAAAG TGAAGATTCC AGAGGCTTGC GCATCAAAAT GTAGGGACAG AGCGAAGCGT 
CACAGTGGTA AAAGCTGTTG CAGGAGTTAT GCAAAAGAGT TATGCAGCCA CCGCCATCAT 
CATCACCACC ACCACCACCA TCACCATGTG AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179501.1
CDS	196..3714
Translation	MALQNKEEEKKKVKKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLLISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLETRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVNDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSIILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRASTSKLNGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSHPGCCGDKKEEKVKPLVKDGCCSEKTRKSEGDMVSLSSCKKSSHVKHDLKMKGGSGCCASKNEKGKEVVAKSCCEKPKQQVESVGDCKSGHCEKKKQAEDIVVPVQIIGHALTHVEIELQTKETCKTSCCDSKEKVKETGLLLSSENTPYLEKGVLIKDEGNCKSGSENMGTVKQSCHEKGCSDEKQTGEITLASEEETDDQDCSSGCCVNEGTVKQSFDEKKHSVLVEKEGLDMETGFCCDAKLVCCGNTEGEVKEQCRLEIKKEEHCKSGCCGEEIQTGEITLVSEEETESTNCSTGCCVDKEEVTQTCHEKPASLVVSGLEVKKDEHCESSHRAVKVETCCKVKIPEACASKCRDRAKRHSGKSCCRSYAKELCSHRHHHHHHHHHHHVSA

Target ORF information:

RefSeq Version	NM_127468.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heavy metal atpase 4 (HMA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127468.5

ORF Insert Sequence:

ATGGCGTTAC AAAACAAAGA AGAAGAGAAA AAGAAAGTGA AGAAGTTGCA AAAGAGTTAC 
TTCGATGTTC TCGGAATCTG TTGTACATCG GAAGTTCCTA TAATCGAGAA TATTCTCAAG 
TCACTTGACG GCGTTAAAGA ATATTCCGTC ATCGTTCCCT CGAGAACCGT GATTGTTGTT 
CACGACAGTC TCCTCATCTC TCCCTTCCAA ATTGCTAAGG CACTAAACGA AGCTAGGTTA 
GAAGCAAACG TGAGGGTAAA CGGAGAAACT AGCTTCAAGA ACAAATGGCC GAGCCCTTTC 
GCCGTAGTTT CCGGCTTACT TCTCCTCCTA TCCTTCCTAA AGTTTGTCTA CTCGCCTTTA 
CGTTGGCTCG CCGTGGCAGC AGTTGCCGCC GGTATCTATC CGATTCTTGC CAAAGCCTTT 
GCTTCCATTA AAAGGCCTAG GATCGACATC AACATATTGG TCATAATAAC CGTGATTGCA 
ACACTTGCAA TGCAAGATTT CATGGAGGCA GCAGCAGTTG TGTTCCTATT CACCATATCC 
GACTGGCTCG AAACAAGAGC TAGCTACAAG GCGACCTCGG TAATGCAGTC TCTGATGAGC 
TTAGCTCCAC AAAAGGCTAT AATAGCAGAG ACTGGTGAAG AAGTTGAAGT AGATGAGGTT 
AAGGTTGATA CAGTTGTAGC AGTTAAAGCT GGTGAAACCA TACCAATTGA TGGAATTGTG 
GTGGATGGAA ACTGTGAGGT AGACGAGAAA ACCTTAACGG GCGAAGCATT TCCTGTGCCT 
AAACAGAGAG ATTCTACCGT TTGGGCTGGA ACCATTAATC TAAATGGTTA CATATGTGTG 
AAAACAACTT CTTTAGCGGG TGATTGTGTG GTTGCGAAAA TGGCTAAGCT AGTAGAAGAA 
GCTCAGAGCA GTAAAACCAA ATCTCAGAGA CTAATAGACA AATGTTCTCA GTACTATACT 
CCAGCAATCA TCTTAGTATC AGCTTGCGTT GCCATTGTCC CGGTTATAAT GAAGGTCCAC 
AACCTTAAAC ATTGGTTCCA CCTAGCATTA GTTGTGTTAG TCAGTGGTTG TCCCTGTGGT 
CTTATCCTCT CTACACCAGT TGCTACTTTC TGTGCACTTA CTAAAGCGGC AACTTCAGGG 
CTTCTGATCA AAAGTGCTGA TTATCTTGAC ACACTCTCAA AGATCAAGAT TGTTGCTTTC 
GATAAAACTG GGACTATTAC AAGAGGAGAG TTCATTGTCA TAGATTTCAA GTCACTCTCT 
AGAGATATAA ACCTACGCAG CTTGCTTTAC TGGGTATCAA GTGTTGAAAG CAAATCAAGT 
CATCCAATGG CAGCAACAAT CGTGGATTAT GCAAAATCTG TTTCTGTTGA GCCTAGGCCT 
GAAGAGGTTG AGGATTACCA GAACTTTCCA GGTGAAGGAA TCTACGGGAA GATTGATGGT 
AACGATATCT TCATTGGGAA CAAAAAGATA GCTTCTCGAG CTGGTTGTTC AACAGTTCCA 
GAGATTGAAG TTGATACCAA AGGCGGGAAG ACTGTTGGAT ACGTCTATGT AGGTGAAAGA 
CTAGCTGGAT TTTTCAATCT TTCTGATGCT TGTAGATCTG GTGTTTCTCA AGCAATGGCA 
GAACTGAAAT CTCTAGGAAT CAAAACCGCA ATGCTAACGG GAGATAATCA AGCCGCGGCA 
ATGCATGCTC AAGAACAGCT AGGGAATGTT TTAGATGTTG TACATGGAGA TCTTCTTCCA 
GAAGATAAGT CCAGAATCAT ACAAGAGTTT AAGAAAGAGG GACCAACCGC AATGGTAGGG 
GACGGTGTGA ATGATGCACC AGCTTTAGCT ACAGCTGATA TTGGTATCTC CATGGGAATT 
TCTGGCTCTG CTCTTGCAAC ACAAACTGGT AATATTATTC TGATGTCTAA TGATATAAGA 
AGGATACCAC AAGCGGTGAA GCTAGCGAGA AGAGCACGAC GCAAAGTTGT TGAAAACGTG 
TGTCTATCGA TCATTTTAAA AGCAGGAATA CTCGCTTTGG CATTTGCTGG TCATCCTTTG 
ATTTGGGCTG CGGTTCTTGT TGATGTAGGG ACTTGTCTGC TTGTGATTTT CAATAGTATG 
TTGCTGCTGC GAGAGAAGAA AAAGATTGGG AACAAAAAGT GTTACAGGGC TTCTACATCT 
AAGTTGAATG GTAGGAAACT TGAAGGCGAT GATGATTATG TTGTGGACTT AGAAGCAGGC 
TTGTTAACAA AGAGCGGGAA TGGTCAATGC AAATCAAGCT GTTGTGGAGA TAAGAAAAAT 
CAAGAGAATG TTGTGATGAT GAAACCAAGT AGTAAAACCA GTTCTGATCA TTCTCACCCT 
GGTTGTTGTG GCGATAAGAA GGAAGAAAAA GTGAAGCCGC TTGTGAAAGA TGGCTGTTGC 
AGTGAGAAAA CTAGGAAATC AGAGGGAGAT ATGGTTTCAT TGAGCTCATG TAAGAAGTCT 
AGTCATGTCA AACATGACCT GAAAATGAAA GGTGGTTCAG GTTGTTGTGC TAGCAAAAAT 
GAGAAAGGGA AGGAAGTAGT GGCAAAGAGC TGTTGTGAGA AACCCAAACA GCAGGTGGAG 
AGTGTTGGAG ACTGCAAGTC TGGTCATTGC GAGAAGAAGA AGCAAGCTGA AGACATTGTT 
GTCCCGGTGC AGATTATTGG TCATGCATTA ACGCATGTGG AGATCGAGTT GCAGACAAAG 
GAAACCTGCA AAACAAGCTG TTGTGACAGT AAAGAAAAGG TTAAGGAGAC AGGTTTGCTG 
CTTTCTAGTG AGAACACACC TTACCTGGAG AAAGGAGTGC TGATTAAAGA TGAAGGAAAC 
TGCAAGTCTG GCAGCGAGAA CATGGGGACA GTGAAACAAA GCTGCCATGA GAAGGGCTGC 
AGCGATGAAA AACAAACCGG GGAAATAACT CTTGCTTCGG AGGAAGAGAC AGATGATCAA 
GATTGCTCCT CGGGATGTTG TGTGAACGAG GGAACAGTGA AACAAAGCTT CGATGAGAAG 
AAGCATTCTG TGTTGGTGGA GAAGGAAGGT TTGGACATGG AAACTGGTTT CTGTTGTGAT 
GCCAAGCTGG TTTGTTGTGG AAACACAGAA GGTGAAGTGA AGGAGCAATG TCGTCTGGAG 
ATAAAGAAAG AAGAACATTG CAAGTCTGGT TGCTGCGGCG AGGAAATACA AACCGGAGAA 
ATCACTCTGG TTTCAGAGGA AGAGACAGAG AGCACGAATT GTTCCACGGG TTGTTGTGTG 
GACAAAGAAG AAGTGACACA AACCTGTCAT GAGAAGCCTG CTAGCTTGGT GGTATCAGGC 
TTGGAAGTGA AGAAGGATGA GCATTGTGAG AGCTCACACA GAGCCGTCAA GGTAGAGACC 
TGTTGCAAAG TGAAGATTCC AGAGGCTTGC GCATCAAAAT GTAGGGACAG AGCGAAGCGT 
CACAGTGGTA AAAGCTGTTG CAGGAGTTAT GCAAAAGAGT TATGCAGCCA CCGCCATCAT 
CATCACCACC ACCACCACCA TCACCATGTG AGTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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HMA4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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