NCU02685 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02685
Entrez Gene ID	3881624
Full Name	pre-mRNA splicing helicase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_960365.2	XP_965458.1	pre-mRNA splicing helicase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	brr2	NP_593346.1
Canis lupus familiaris (dog)	SNRNP200	XP_532949.2
Bos taurus (cattle)	SNRNP200	NP_001193092.1
Caenorhabditis elegans (roundworm)	snrp-200	NP_496710.1
Kluyveromyces lactis	KLLA0C17798g	XP_452995.1
Arabidopsis thaliana (thale cress)	AT2G42270	NP_181756.1
Pan troglodytes (chimpanzee)	SNRNP200	XP_001147672.1
Saccharomyces cerevisiae (baker's yeast)	BRR2	NP_011099.1
Eremothecium gossypii	AGOS_AGR113W	NP_986779.1
Neurospora crassa	NCU02685	XP_965458.1
Xenopus tropicalis (tropical clawed frog)	snrnp200	XP_002932581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001519	XP_321605.3
Magnaporthe oryzae (rice blast fungus)	MGG_15536	XP_003713506.1
Homo sapiens (human)	SNRNP200	NP_054733.2
	SNRNP200	XP_001098299.2
Mus musculus (house mouse)	Snrnp200	NP_796188.2
Rattus norvegicus (Norway rat)	Snrnp200	NP_001032855.2
Danio rerio (zebrafish)	snrnp200	NP_001116729.1
Drosophila melanogaster (fruit fly)	l(3)72Ab	NP_648818.3
Arabidopsis thaliana (thale cress)	emb1507	NP_001185050.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02685 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02685 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf08731	XM_960365.2 Latest version!	Neurospora crassa OR74A pre-mRNA splicing helicase (NCU02685), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02685 ( XM_960365.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_965458.1 Neurospora crassa OR74A pre-mRNA splicing helicase (NCU02685), mRNA.

Price & Availability↑

CloneID	ONf08731
Clone ID Related Accession (Same CDS sequence)	XM_960365.2
Accession Version	XM_960365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	pre-mRNA splicing helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960365.1.

ATGACGGACC ACCACCGCGA TGTGTCGCAA TATAAATACT CGGCCATGTC CAACCTGGTT 
CTCCAGGCGG ACCGTCGTTT CGTCACGAGG CGTACCGATG AGGCCACGGG AGACCCCGAG 
TCCCTAGCCG GGCGTCTGTC CATCAAAGAT ATGGGCTCCA GAGTCGCTCG CGACTCAGCA 
CCAAAGCCCA AGAAGGCGGG CGCCATGCCC GACGTCGAGC GGGGCAGTCT AAGGGAAGGT 
GCTGATATCC TGCAACGAGA GAAGAAGAAG GGCAAACTCG AGTCCGCTAC AAGAGGTGGA 
GGTATTCTTA CTGGCGCCGA CGCGCTTATC GAAGGTCTCC GCTACCGCCC TCGAACGCAG 
CCGACGCGCG ATGCCTTCAA CCTTATCCTC ACCATTGTCG CCGAACACTT GGGCGACGTG 
CCCCATGAGG TCGTTCGCAG TGCTGCTGAC GCGGTTCTCG AATATCTCAA GGATGACGAC 
CTGAAGGATT TTGACAAGAA GAAGGAGGTT GATGATATTC TTGGAGTCTC GATGGGGCCC 
AAGCAGTTCA ACGAGCTCAT AAATCTCGGC AAAAAGATCA CGGATTACGA CGCTCAAGAT 
GAAGACGAGG ATGCGGGCGA TGCTAGGCAA GAAGGCGACG ACGAGATTGA CGGTCGCCAG 
GGTGTTGCGG TCAACTTCGA GAACGATGAA GATGATGATG GCATGGTTGA TGTTGTTCGG 
GACGAATCTT CCGATGAGGA GGATTTTGCG GAGGATGAAG AGCTGCCTGA CAGCAACGAG 
GTGGTCGATG GTGAAGGAGG TCAGGAGGAG GTGGTCGAAG ATATTCTGAA CGGCGAGGCC 
ATGGTCATCG ATTCAGCACC GGAAGGGAAG AAAAGCGGGT CTGACGACAA TTCCATACCC 
GCCAGAGATA TCGATGCGTT CTGGTTACAA CGCCAGATTG GCCGGCTATA TCCCGACGCA 
CATATCCAGC ATGATAAGAC AATGTCTGCG CTCAAGACTC TTTCCGGCGA GCCCGATGAG 
CCAGGTGGTG AAGAAAAGCA GCTCCGCGAC ATCGAAAACG ACTTGATGGA GCTTTTTGAT 
TACGAACACC ACGAGCTTGT GCAAAAACTC ATTGCTAACC GGGAGAAGGT CGTATGGCTT 
ACTCGGCTGG CCAGAGCTGA GAATGAGGAG GAGAGAAATA CGATCAAGCG GGAGATGGCT 
TCTGAGGGTC TGCGTTGGAT TCTGGACGAG CTGGAAGGCA AGACCGACGG CGGCCAGAAG 
AAGATCAAGA TGGACATCAA GATGGACATT GACTCGGGGG CCTTTGCTGA TAAGGAAGGC 
CAAAAGCCTG AGCGTCCCGA GGGACAGCTA GTCGGAGGTC TGCAGCCACG CAAGTTAATA 
AACCTGGAGA ACCTCATATT CGATCAGGGT AACCACCTTA TGACCAATCC CAAAGTACGA 
CTTCCCGAGG GCTCGACAAA GCGCACCTTC AAGGGCTATG AAGAAATCCA TGTGCCCCCA 
CCAAAGAAGC GCAACGACCC CAGCGACGCC CATGTCCCGA TTTCTGAAAT GCCTGAGTGG 
GCTCAGATAC CTTTCAGCAC GGCCAAATCG TTAAACAAGA TTCAGTCAAA GTGCTATCCT 
ACTGCCTTTG GTGATGATGG AAACATGCTT GTCTGTGCTC CGACGGGCTC TGGCAAGACC 
AACGTGGCGA TGCTCACCAT CCTTCGTGAG ATCGGGAAGC ATAGAAACGA AGCCGGCGAG 
ATCGACCTGG ACGCCTTCAA GATCGTCTAT ATCGCGCCCT TGAAAGCCCT TGTTCAAGAA 
CAGGTCGGCA ACTTCGGAAA GCGTCTCGAA CCATTCGGTA TCAGGGTGGC CGAACTCACT 
GGCGATCGGC AACTTACTAA GCAACAGATC TCGGAAACGC AGATTATCGT TACGACCCCC 
GAAAAATGGG ATGTCATCAC GAGAAAGGCT ACTGATCTCT CCTACACCAA CCTGGTCCGC 
CTGATTATTA TTGACGAGAT CCATCTCCTT CACGATGACC GTGGTCCTGT GCTTGAAAGC 
ATTGTCGCCA GAACCATCCG CAAGACAGAG CAGACCGGTG AGCCTGTTCG CATTGTGGGC 
TTGTCTGCCA CTCTGCCCAA CTACCGCGAC GTAGCGAGCT TCCTGCGTGT GGATACGAAC 
ACCGGGCTCT TCCACTTCGA TGGTACCTTC CGCCCTTGCC CCCTCCGTCA AGAGTTTATC 
GGCGTCACGG ACAGAAAGGC CATCAAGCAG TTAAAGACCA TGAATGATAT CACCTACCAA 
AAGGTCTTGG AGCACGTAGG ACAGAATCGC AATCAGATGC TCATCTTTGT CCATTCCAGA 
AAGGAAACGG CAAAGACCGC CAGGTACATC AGGGACAAGG CTTTGGAAAT GGACACCATC 
AACCAGATCT TGAAGCACGA CGCCGGAACC CGTGAAGTCC TGAGCGAAGC CTCCAACTCT 
GTCAACAACA CCGATCTGAA GGATATCTTG CCCTATGGCT TCGGTATCCA TCATGCTGGT 
ATGAGTCGTG CTGATCGTAC CGACGTCGAA GATTTGTTTG CCAGTGGCCA TATCCAGGTT 
CTTGTGTGCA CAGCCACTCT TGCTTGGGGT GTCAACCTTC CAGCCCATAC CGTCATCATC 
AAGGGCACGC AAGTGTACTC GCCTGAAAAA GGTAGCTGGG TTGAGCTCAG TCCTCAGGAT 
GTGCTGCAGA TGCTTGGTCG TGCCGGTCGT CCTCAGTTCG ACACCTATGG TGAAGGTATC 
ATCATCACCA CCCAGGGAGA GATGCAGTAC TACCTTTCGC TCCTTAACCA GCAGTTACCC 
ATTGAAAGTC AATTTGCTAG CAAGTTGGTT GATAACCTCA ATGCTGAAAT CGTGCTGGGC 
AATGTCAGAA GCCGTGACGA AGGTGTAGAG TGGCTTGGCT ATACCTACCT CTTTGTTCGT 
ATGCTTCGCT CTCCTGGTCT TTACCAAGTT GGGGCAGAGT ACGAGGATGA TGAAGCTTTG 
GAGCAAAAAC GTGTCGACCT AATACATTCC GCTGCTACTG TGCTGAAAAA GTCAAATCTA 
ATCAAGTATG ACGAGAAGAC CGGAAAGCTT CAGTCCACTG AACTTGGCCG CATCGCCTCG 
CACTACTACA TCAGCTATGG TTCGATGGAT ACCTACAACA AGTTGATTCA ACCCTCTATC 
ACCGATGTCG AACTCTTCCG AGTATTCGCT CAGAGCGCCG AATTCAAGTA CATTCCTGTT 
CGGCAAGAAG AAAAGCTGGA ATTGGCCAAG CTCCTGGCAA AGGTACCCAT TCCGGTCAAG 
GAGAGCATTG AGGAGCCTAC CGCAAAGATC AACGTGTTGT TACAGGCGTT CATCTCTCGT 
CTGAAGCTGG AGGGTCTCGC CCTCATGGCT GACATGGTCT ACGTTACGCA ATCTGCTGGT 
CGTATTCTTC GGGCTATCTT TGAAATCACG ATGAAGAAGG GATGGGCATC AGTTGCTAAG 
CTGGCCCTGA ACCTGTGCAA GATGGCCGAG AAGAGGATGT GGCCAACCAT GTCGCCGCTG 
AGACAGTTCC CCAACTGTCC TGTTGAGATT ATCAGGAAGG CTGAAAGGAT CGACGTGCCC 
TTTTCCAGCT ACTTCGATCT GGACCCTCCT CGGATGGGCG AGCTTCTCGG ACTACCCAAG 
GCGGGCAAGA CTGTTTGCAG CCTGGTGGCC AAGTTCCCCC GCGTCGAGGT CCAAGCCCAG 
GTGCAGCCCA TGACAAGGTC GATGCTGCGC ATTGAGCTTG CGATCACGCC AAACTTCGAA 
TGGGATGCCG ATATCCATGG GCTTTCCGAG AGCTTTTGGA TTATCGTAGA AGATTGCGAT 
GGGGAAGACA TTCTTTTCCA TGATCAGTTC ATCCTGCGCA AGGACTATGC GGAGTCCGAG 
TCCAACGAAC ACATTGTTGA ATTCACTGTA CCCATTACTG AGCCCATGCC CCCCAACTAT 
TTTATCTCCG TCATTTCCGA CAGGTGGATG CATTCCGAGA CACGTCTGCC CGTGTCGTTC 
AGGAAACTCA TTCTCCCAGA GAGATTCCCT CCTCACACCG AACTGCTTGA CCTGCAACCA 
CTACCGGTCA ATGCCTTGAA GGCCAAGGAC TATTCCGCGC TGTATCCTGA TTGGCAACAA 
TTCAACAAGG TGCAAACACA GACTTTCAAG TCCCTGTACG AGACTGACAA CAACGTCCTC 
ATCTGCTCAC CAACGGGCAG TGGCAAGACT GTTTGTGCCG AGTTTGCCTT GCTGCGCCAC 
TGGGCTAAGA AGGAACACGG CCGGGCCGTC TACATTGCAC CTTTCCAGGA ACTCGTAGAT 
CTGCGATTCC AGGACTGGCA GAAGCGGTTT GCTAACCTCC GCGGCGGCAA GGACATTGTG 
AAGCTTACAG GAGAGACGAC CACTGACCTT AGGCTCCTGG AGCAAGGCGA CCTCATCATG 
GCCACACCGC TGCAGTGGGA TGTCCTGTCG CGGCAGTGGA AGAGGAGGAA GAACGTTCAG 
ACGGTAGAGC TTTTCATCGC TGACGAGCTG CACTTACTTG GTGGCCAGAT GGGCTACGTC 
TACGAGATCA TCGTCTCGCG GATGCACTAT ATCAGGACCC AAACGGAGTT GCCACTAAGG 
ATTGTCGGCC TCAGTGTGTC TCTTGCCAAC GCTCGTGATG TTGGCGAATG GATCGACGCG 
AAAAAGCACG ACATTTACAA CTTCAGCCCG CATGTGCGAC CTATTCCATT GGAATTGCAC 
ATTCAGTCAT ACTCCATCCC CCACTTTCCG TCCCTCATGC TGGCGATGGC AAAGCCTACT 
TATCTGGCCG TTACTCAGCT TTCTCCCGAC CAATCTGCTC TCATCTTCGT GCCCAGTCGG 
AAGCAAACAA GAGCCACGGC ACGAGATATC CTCACCGCCT GCTTGGCTGA CGATGATGAA 
GATCGTTTCC TTCATGTAGA CGTTGAGCAG ATCCAAAAGC TCCTTGATCG CGTTCAAGAA 
GCGGCATTAG CTGAGGCCCT GAAGCACGGT GTTGGTTACT ACCACGAAGC CCTGAGTCTC 
AATGACAAGC GCATCGTCAA GCATCTCTAT AACAACGGCG CCATCCAGGT GCTGATCGCC 
TCTCGCGATG TGTGCTGGGA GCTTGATTGC ACTGCTCATC TGGTAGTTGT TATGGGCACC 
CAGTATTTCG AGGGCAGGGA GCACCGCTAT GTTGATTATC CCCTGAGCGA GGTTTTGCAA 
ATGTTTGGCA AGGCCCTCCA GCAGAATAAG GCTGGGCGTG GTTGCGGTGT CTTGATGGTT 
CCTGCGGTCA AGCGGGAGTA TTACAAGAAG TTCCTCAACG AAGCCCTCCC TGTCGAGTCT 
CATCTGCACA ATTTCCTCCA CGATGCCTTC GTGACAGAAA TCAGCACCAA GATGATTGAG 
TCAGGTGAAG ATGCCATCAA CTGGGCTACT TTTACTTACT TCTACCGCCG CCTGCTCGCC 
AACCCAAGCT ACTACAGTTT GACAGACCCT ACGCACGACG GTCTCAGCCA GTATCTCTCC 
GACATGGTTG AGGCTACCTT GAAGGATCTG GCCGAGTCCA AGATCATCGA CTTCGATGAG 
GATGACGGCA CCGTTGCTCC TCAGAACGCC GCCATGATTG CTGCTTACTA CAACATCTCG 
TACATTACGA TGCAAACCTT CCTTCTTTCG CTGACGGCAA AGACAAAGCT GAAGGGTATC 
CTCGAGATCG TGACGTCGGC TACCGAGTTT GAAGCGATCC AGATCAGAAG ACATGAAGAA 
GTCATTCTGC GTCGCATTTA CGAGAGTGTC CCCGTCAAGA TGGCCGAGCC TGTCTTTGAT 
TCACCGCATT TCAAGGCCTT CGTTCTCTTG CAGGCCCACT TCTCCAGGAT GAACCTCCCC 
ATCGATCTCG CAAAGGATCA AGAGGTCATC CTCACCAAGG TGCTCAGCCT GCTGAGCGCT 
ACTGTTGACA TTCTCTCGTC GGATGGTCAC CTCAACGCCA TGAACGCCAT GGAAATGTCC 
CAGATGGTGG TGCAAGCGAT GTGGGATCGG GATAGCCCGC TCAAGCAGAT TCCCAACTTC 
ACGACGGAGG TCATCAAAAC GGCCAACAAG TACGGTATCC GAGACATCTT CGACTTTATG 
GAGAAGATGA ACCCCGAGGA GAACGCCGAC TACGCCTCTC TGGTCAGGGA CCTTGGTCTG 
TCTCAGGCCC AGCTCGCCCA GGCTGCCGAG TTCACCAACA ACAAGTACCC CGACGTCTCG 
TTGGAGTTCG AGCTTGAAGA CAAGGACAAC ATTCGGGCGA ACGAGCCTGC ATACCTCAAG 
ATCAACATTG AGCGCGAGGT TGATGAGGAC GAGGAGTTTG ATCCTACGGT GCACGCTCCG 
TTTTACCCTG GCAAGAAGAC GGAGAATTGG TGGTTGGTGG TTGGCGAGGA GAGCTCCAAG 
ACTCTGCTGG CGATCAAGCG GGTCACCATT GGCAAGAAGC TCAACGTCAG GCTGGAGTTT 
ACGGTGCCTA CGCCTGGTCG CCACGACCTG AAGCTAATGT TGATGAGCGA CAGCTATGTC 
GGTGTTGATC AGGATCCGGC GTTTTCGGTC ATGGTGGAGG AGGGAATGGA TGTTGATGAG 
AGCGACGAGG AGGAGGAAGA GGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_965458.1
CDS	115..6744
Translation	MTDHHRDVSQYKYSAMSNLVLQADRRFVTRRTDEATGDPESLAGRLSIKDMGSRVARDSAPKPKKAGAMPDVERGSLREGADILQREKKKGKLESATRGGGILTGADALIEGLRYRPRTQPTRDAFNLILTIVAEHLGDVPHEVVRSAADAVLEYLKDDDLKDFDKKKEVDDILGVSMGPKQFNELINLGKKITDYDAQDEDEDAGDARQEGDDEIDGRQGVAVNFENDEDDDGMVDVVRDESSDEEDFAEDEELPDSNEVVDGEGGQEEVVEDILNGEAMVIDSAPEGKKSGSDDNSIPARDIDAFWLQRQIGRLYPDAHIQHDKTMSALKTLSGEPDEPGGEEKQLRDIENDLMELFDYEHHELVQKLIANREKVVWLTRLARAENEEERNTIKREMASEGLRWILDELEGKTDGGQKKIKMDIKMDIDSGAFADKEGQKPERPEGQLVGGLQPRKLINLENLIFDQGNHLMTNPKVRLPEGSTKRTFKGYEEIHVPPPKKRNDPSDAHVPISEMPEWAQIPFSTAKSLNKIQSKCYPTAFGDDGNMLVCAPTGSGKTNVAMLTILREIGKHRNEAGEIDLDAFKIVYIAPLKALVQEQVGNFGKRLEPFGIRVAELTGDRQLTKQQISETQIIVTTPEKWDVITRKATDLSYTNLVRLIIIDEIHLLHDDRGPVLESIVARTIRKTEQTGEPVRIVGLSATLPNYRDVASFLRVDTNTGLFHFDGTFRPCPLRQEFIGVTDRKAIKQLKTMNDITYQKVLEHVGQNRNQMLIFVHSRKETAKTARYIRDKALEMDTINQILKHDAGTREVLSEASNSVNNTDLKDILPYGFGIHHAGMSRADRTDVEDLFASGHIQVLVCTATLAWGVNLPAHTVIIKGTQVYSPEKGSWVELSPQDVLQMLGRAGRPQFDTYGEGIIITTQGEMQYYLSLLNQQLPIESQFASKLVDNLNAEIVLGNVRSRDEGVEWLGYTYLFVRMLRSPGLYQVGAEYEDDEALEQKRVDLIHSAATVLKKSNLIKYDEKTGKLQSTELGRIASHYYISYGSMDTYNKLIQPSITDVELFRVFAQSAEFKYIPVRQEEKLELAKLLAKVPIPVKESIEEPTAKINVLLQAFISRLKLEGLALMADMVYVTQSAGRILRAIFEITMKKGWASVAKLALNLCKMAEKRMWPTMSPLRQFPNCPVEIIRKAERIDVPFSSYFDLDPPRMGELLGLPKAGKTVCSLVAKFPRVEVQAQVQPMTRSMLRIELAITPNFEWDADIHGLSESFWIIVEDCDGEDILFHDQFILRKDYAESESNEHIVEFTVPITEPMPPNYFISVISDRWMHSETRLPVSFRKLILPERFPPHTELLDLQPLPVNALKAKDYSALYPDWQQFNKVQTQTFKSLYETDNNVLICSPTGSGKTVCAEFALLRHWAKKEHGRAVYIAPFQELVDLRFQDWQKRFANLRGGKDIVKLTGETTTDLRLLEQGDLIMATPLQWDVLSRQWKRRKNVQTVELFIADELHLLGGQMGYVYEIIVSRMHYIRTQTELPLRIVGLSVSLANARDVGEWIDAKKHDIYNFSPHVRPIPLELHIQSYSIPHFPSLMLAMAKPTYLAVTQLSPDQSALIFVPSRKQTRATARDILTACLADDDEDRFLHVDVEQIQKLLDRVQEAALAEALKHGVGYYHEALSLNDKRIVKHLYNNGAIQVLIASRDVCWELDCTAHLVVVMGTQYFEGREHRYVDYPLSEVLQMFGKALQQNKAGRGCGVLMVPAVKREYYKKFLNEALPVESHLHNFLHDAFVTEISTKMIESGEDAINWATFTYFYRRLLANPSYYSLTDPTHDGLSQYLSDMVEATLKDLAESKIIDFDEDDGTVAPQNAAMIAAYYNISYITMQTFLLSLTAKTKLKGILEIVTSATEFEAIQIRRHEEVILRRIYESVPVKMAEPVFDSPHFKAFVLLQAHFSRMNLPIDLAKDQEVILTKVLSLLSATVDILSSDGHLNAMNAMEMSQMVVQAMWDRDSPLKQIPNFTTEVIKTANKYGIRDIFDFMEKMNPEENADYASLVRDLGLSQAQLAQAAEFTNNKYPDVSLEFELEDKDNIRANEPAYLKINIEREVDEDEEFDPTVHAPFYPGKKTENWWLVVGEESSKTLLAIKRVTIGKKLNVRLEFTVPTPGRHDLKLMLMSDSYVGVDQDPAFSVMVEEGMDVDESDEEEEEEE

Target ORF information:

RefSeq Version	XM_960365.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A pre-mRNA splicing helicase (NCU02685), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_960365.2

ORF Insert Sequence:

ATGACGGACC ACCACCGCGA TGTGTCGCAA TATAAATACT CGGCCATGTC CAACCTGGTT 
CTCCAGGCGG ACCGTCGTTT CGTCACGAGG CGTACCGATG AGGCCACGGG AGACCCCGAG 
TCCCTAGCCG GGCGTCTGTC CATCAAAGAT ATGGGCTCCA GAGTCGCTCG CGACTCAGCA 
CCAAAGCCCA AGAAGGCGGG CGCCATGCCC GACGTCGAGC GGGGCAGTCT AAGGGAAGGT 
GCTGATATCC TGCAACGAGA GAAGAAGAAG GGCAAACTCG AGTCCGCTAC AAGAGGTGGA 
GGTATTCTTA CTGGCGCCGA CGCGCTTATC GAAGGTCTCC GCTACCGCCC TCGAACGCAG 
CCGACGCGCG ATGCCTTCAA CCTTATCCTC ACCATTGTCG CCGAACACTT GGGCGACGTG 
CCCCATGAGG TCGTTCGCAG TGCTGCTGAC GCGGTTCTCG AATATCTCAA GGATGACGAC 
CTGAAGGATT TTGACAAGAA GAAGGAGGTT GATGATATTC TTGGAGTCTC GATGGGGCCC 
AAGCAGTTCA ACGAGCTCAT AAATCTCGGC AAAAAGATCA CGGATTACGA CGCTCAAGAT 
GAAGACGAGG ATGCGGGCGA TGCTAGGCAA GAAGGCGACG ACGAGATTGA CGGTCGCCAG 
GGTGTTGCGG TCAACTTCGA GAACGATGAA GATGATGATG GCATGGTTGA TGTTGTTCGG 
GACGAATCTT CCGATGAGGA GGATTTTGCG GAGGATGAAG AGCTGCCTGA CAGCAACGAG 
GTGGTCGATG GTGAAGGAGG TCAGGAGGAG GTGGTCGAAG ATATTCTGAA CGGCGAGGCC 
ATGGTCATCG ATTCAGCACC GGAAGGGAAG AAAAGCGGGT CTGACGACAA TTCCATACCC 
GCCAGAGATA TCGATGCGTT CTGGTTACAA CGCCAGATTG GCCGGCTATA TCCCGACGCA 
CATATCCAGC ATGATAAGAC AATGTCTGCG CTCAAGACTC TTTCCGGCGA GCCCGATGAG 
CCAGGTGGTG AAGAAAAGCA GCTCCGCGAC ATCGAAAACG ACTTGATGGA GCTTTTTGAT 
TACGAACACC ACGAGCTTGT GCAAAAACTC ATTGCTAACC GGGAGAAGGT CGTATGGCTT 
ACTCGGCTGG CCAGAGCTGA GAATGAGGAG GAGAGAAATA CGATCAAGCG GGAGATGGCT 
TCTGAGGGTC TGCGTTGGAT TCTGGACGAG CTGGAAGGCA AGACCGACGG CGGCCAGAAG 
AAGATCAAGA TGGACATCAA GATGGACATT GACTCGGGGG CCTTTGCTGA TAAGGAAGGC 
CAAAAGCCTG AGCGTCCCGA GGGACAGCTA GTCGGAGGTC TGCAGCCACG CAAGTTAATA 
AACCTGGAGA ACCTCATATT CGATCAGGGT AACCACCTTA TGACCAATCC CAAAGTACGA 
CTTCCCGAGG GCTCGACAAA GCGCACCTTC AAGGGCTATG AAGAAATCCA TGTGCCCCCA 
CCAAAGAAGC GCAACGACCC CAGCGACGCC CATGTCCCGA TTTCTGAAAT GCCTGAGTGG 
GCTCAGATAC CTTTCAGCAC GGCCAAATCG TTAAACAAGA TTCAGTCAAA GTGCTATCCT 
ACTGCCTTTG GTGATGATGG AAACATGCTT GTCTGTGCTC CGACGGGCTC TGGCAAGACC 
AACGTGGCGA TGCTCACCAT CCTTCGTGAG ATCGGGAAGC ATAGAAACGA AGCCGGCGAG 
ATCGACCTGG ACGCCTTCAA GATCGTCTAT ATCGCGCCCT TGAAAGCCCT TGTTCAAGAA 
CAGGTCGGCA ACTTCGGAAA GCGTCTCGAA CCATTCGGTA TCAGGGTGGC CGAACTCACT 
GGCGATCGGC AACTTACTAA GCAACAGATC TCGGAAACGC AGATTATCGT TACGACCCCC 
GAAAAATGGG ATGTCATCAC GAGAAAGGCT ACTGATCTCT CCTACACCAA CCTGGTCCGC 
CTGATTATTA TTGACGAGAT CCATCTCCTT CACGATGACC GTGGTCCTGT GCTTGAAAGC 
ATTGTCGCCA GAACCATCCG CAAGACAGAG CAGACCGGTG AGCCTGTTCG CATTGTGGGC 
TTGTCTGCCA CTCTGCCCAA CTACCGCGAC GTAGCGAGCT TCCTGCGTGT GGATACGAAC 
ACCGGGCTCT TCCACTTCGA TGGTACCTTC CGCCCTTGCC CCCTCCGTCA AGAGTTTATC 
GGCGTCACGG ACAGAAAGGC CATCAAGCAG TTAAAGACCA TGAATGATAT CACCTACCAA 
AAGGTCTTGG AGCACGTAGG ACAGAATCGC AATCAGATGC TCATCTTTGT CCATTCCAGA 
AAGGAAACGG CAAAGACCGC CAGGTACATC AGGGACAAGG CTTTGGAAAT GGACACCATC 
AACCAGATCT TGAAGCACGA CGCCGGAACC CGTGAAGTCC TGAGCGAAGC CTCCAACTCT 
GTCAACAACA CCGATCTGAA GGATATCTTG CCCTATGGCT TCGGTATCCA TCATGCTGGT 
ATGAGTCGTG CTGATCGTAC CGACGTCGAA GATTTGTTTG CCAGTGGCCA TATCCAGGTT 
CTTGTGTGCA CAGCCACTCT TGCTTGGGGT GTCAACCTTC CAGCCCATAC CGTCATCATC 
AAGGGCACGC AAGTGTACTC GCCTGAAAAA GGTAGCTGGG TTGAGCTCAG TCCTCAGGAT 
GTGCTGCAGA TGCTTGGTCG TGCCGGTCGT CCTCAGTTCG ACACCTATGG TGAAGGTATC 
ATCATCACCA CCCAGGGAGA GATGCAGTAC TACCTTTCGC TCCTTAACCA GCAGTTACCC 
ATTGAAAGTC AATTTGCTAG CAAGTTGGTT GATAACCTCA ATGCTGAAAT CGTGCTGGGC 
AATGTCAGAA GCCGTGACGA AGGTGTAGAG TGGCTTGGCT ATACCTACCT CTTTGTTCGT 
ATGCTTCGCT CTCCTGGTCT TTACCAAGTT GGGGCAGAGT ACGAGGATGA TGAAGCTTTG 
GAGCAAAAAC GTGTCGACCT AATACATTCC GCTGCTACTG TGCTGAAAAA GTCAAATCTA 
ATCAAGTATG ACGAGAAGAC CGGAAAGCTT CAGTCCACTG AACTTGGCCG CATCGCCTCG 
CACTACTACA TCAGCTATGG TTCGATGGAT ACCTACAACA AGTTGATTCA ACCCTCTATC 
ACCGATGTCG AACTCTTCCG AGTATTCGCT CAGAGCGCCG AATTCAAGTA CATTCCTGTT 
CGGCAAGAAG AAAAGCTGGA ATTGGCCAAG CTCCTGGCAA AGGTACCCAT TCCGGTCAAG 
GAGAGCATTG AGGAGCCTAC CGCAAAGATC AACGTGTTGT TACAGGCGTT CATCTCTCGT 
CTGAAGCTGG AGGGTCTCGC CCTCATGGCT GACATGGTCT ACGTTACGCA ATCTGCTGGT 
CGTATTCTTC GGGCTATCTT TGAAATCACG ATGAAGAAGG GATGGGCATC AGTTGCTAAG 
CTGGCCCTGA ACCTGTGCAA GATGGCCGAG AAGAGGATGT GGCCAACCAT GTCGCCGCTG 
AGACAGTTCC CCAACTGTCC TGTTGAGATT ATCAGGAAGG CTGAAAGGAT CGACGTGCCC 
TTTTCCAGCT ACTTCGATCT GGACCCTCCT CGGATGGGCG AGCTTCTCGG ACTACCCAAG 
GCGGGCAAGA CTGTTTGCAG CCTGGTGGCC AAGTTCCCCC GCGTCGAGGT CCAAGCCCAG 
GTGCAGCCCA TGACAAGGTC GATGCTGCGC ATTGAGCTTG CGATCACGCC AAACTTCGAA 
TGGGATGCCG ATATCCATGG GCTTTCCGAG AGCTTTTGGA TTATCGTAGA AGATTGCGAT 
GGGGAAGACA TTCTTTTCCA TGATCAGTTC ATCCTGCGCA AGGACTATGC GGAGTCCGAG 
TCCAACGAAC ACATTGTTGA ATTCACTGTA CCCATTACTG AGCCCATGCC CCCCAACTAT 
TTTATCTCCG TCATTTCCGA CAGGTGGATG CATTCCGAGA CACGTCTGCC CGTGTCGTTC 
AGGAAACTCA TTCTCCCAGA GAGATTCCCT CCTCACACCG AACTGCTTGA CCTGCAACCA 
CTACCGGTCA ATGCCTTGAA GGCCAAGGAC TATTCCGCGC TGTATCCTGA TTGGCAACAA 
TTCAACAAGG TGCAAACACA GACTTTCAAG TCCCTGTACG AGACTGACAA CAACGTCCTC 
ATCTGCTCAC CAACGGGCAG TGGCAAGACT GTTTGTGCCG AGTTTGCCTT GCTGCGCCAC 
TGGGCTAAGA AGGAACACGG CCGGGCCGTC TACATTGCAC CTTTCCAGGA ACTCGTAGAT 
CTGCGATTCC AGGACTGGCA GAAGCGGTTT GCTAACCTCC GCGGCGGCAA GGACATTGTG 
AAGCTTACAG GAGAGACGAC CACTGACCTT AGGCTCCTGG AGCAAGGCGA CCTCATCATG 
GCCACACCGC TGCAGTGGGA TGTCCTGTCG CGGCAGTGGA AGAGGAGGAA GAACGTTCAG 
ACGGTAGAGC TTTTCATCGC TGACGAGCTG CACTTACTTG GTGGCCAGAT GGGCTACGTC 
TACGAGATCA TCGTCTCGCG GATGCACTAT ATCAGGACCC AAACGGAGTT GCCACTAAGG 
ATTGTCGGCC TCAGTGTGTC TCTTGCCAAC GCTCGTGATG TTGGCGAATG GATCGACGCG 
AAAAAGCACG ACATTTACAA CTTCAGCCCG CATGTGCGAC CTATTCCATT GGAATTGCAC 
ATTCAGTCAT ACTCCATCCC CCACTTTCCG TCCCTCATGC TGGCGATGGC AAAGCCTACT 
TATCTGGCCG TTACTCAGCT TTCTCCCGAC CAATCTGCTC TCATCTTCGT GCCCAGTCGG 
AAGCAAACAA GAGCCACGGC ACGAGATATC CTCACCGCCT GCTTGGCTGA CGATGATGAA 
GATCGTTTCC TTCATGTAGA CGTTGAGCAG ATCCAAAAGC TCCTTGATCG CGTTCAAGAA 
GCGGCATTAG CTGAGGCCCT GAAGCACGGT GTTGGTTACT ACCACGAAGC CCTGAGTCTC 
AATGACAAGC GCATCGTCAA GCATCTCTAT AACAACGGCG CCATCCAGGT GCTGATCGCC 
TCTCGCGATG TGTGCTGGGA GCTTGATTGC ACTGCTCATC TGGTAGTTGT TATGGGCACC 
CAGTATTTCG AGGGCAGGGA GCACCGCTAT GTTGATTATC CCCTGAGCGA GGTTTTGCAA 
ATGTTTGGCA AGGCCCTCCA GCAGAATAAG GCTGGGCGTG GTTGCGGTGT CTTGATGGTT 
CCTGCGGTCA AGCGGGAGTA TTACAAGAAG TTCCTCAACG AAGCCCTCCC TGTCGAGTCT 
CATCTGCACA ATTTCCTCCA CGATGCCTTC GTGACAGAAA TCAGCACCAA GATGATTGAG 
TCAGGTGAAG ATGCCATCAA CTGGGCTACT TTTACTTACT TCTACCGCCG CCTGCTCGCC 
AACCCAAGCT ACTACAGTTT GACAGACCCT ACGCACGACG GTCTCAGCCA GTATCTCTCC 
GACATGGTTG AGGCTACCTT GAAGGATCTG GCCGAGTCCA AGATCATCGA CTTCGATGAG 
GATGACGGCA CCGTTGCTCC TCAGAACGCC GCCATGATTG CTGCTTACTA CAACATCTCG 
TACATTACGA TGCAAACCTT CCTTCTTTCG CTGACGGCAA AGACAAAGCT GAAGGGTATC 
CTCGAGATCG TGACGTCGGC TACCGAGTTT GAAGCGATCC AGATCAGAAG ACATGAAGAA 
GTCATTCTGC GTCGCATTTA CGAGAGTGTC CCCGTCAAGA TGGCCGAGCC TGTCTTTGAT 
TCACCGCATT TCAAGGCCTT CGTTCTCTTG CAGGCCCACT TCTCCAGGAT GAACCTCCCC 
ATCGATCTCG CAAAGGATCA AGAGGTCATC CTCACCAAGG TGCTCAGCCT GCTGAGCGCT 
ACTGTTGACA TTCTCTCGTC GGATGGTCAC CTCAACGCCA TGAACGCCAT GGAAATGTCC 
CAGATGGTGG TGCAAGCGAT GTGGGATCGG GATAGCCCGC TCAAGCAGAT TCCCAACTTC 
ACGACGGAGG TCATCAAAAC GGCCAACAAG TACGGTATCC GAGACATCTT CGACTTTATG 
GAGAAGATGA ACCCCGAGGA GAACGCCGAC TACGCCTCTC TGGTCAGGGA CCTTGGTCTG 
TCTCAGGCCC AGCTCGCCCA GGCTGCCGAG TTCACCAACA ACAAGTACCC CGACGTCTCG 
TTGGAGTTCG AGCTTGAAGA CAAGGACAAC ATTCGGGCGA ACGAGCCTGC ATACCTCAAG 
ATCAACATTG AGCGCGAGGT TGATGAGGAC GAGGAGTTTG ATCCTACGGT GCACGCTCCG 
TTTTACCCTG GCAAGAAGAC GGAGAATTGG TGGTTGGTGG TTGGCGAGGA GAGCTCCAAG 
ACTCTGCTGG CGATCAAGCG GGTCACCATT GGCAAGAAGC TCAACGTCAG GCTGGAGTTT 
ACGGTGCCTA CGCCTGGTCG CCACGACCTG AAGCTAATGT TGATGAGCGA CAGCTATGTC 
GGTGTTGATC AGGATCCGGC GTTTTCGGTC ATGGTGGAGG AGGGAATGGA TGTTGATGAG 
AGCGACGAGG AGGAGGAAGA GGAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02685 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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