NCU03220 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03220
Entrez Gene ID	3881469
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394812.1	XP_011393114.1	uncharacterized protein
XM_011394814.1	XP_011393116.1	uncharacterized protein
XM_011394813.1	XP_011393115.1	hypothetical protein

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A09691g	XP_451423.1
Saccharomyces cerevisiae (baker's yeast)	YGR266W	NP_011782.1
Neurospora crassa	NCU03220	XP_965320.1
Magnaporthe oryzae (rice blast fungus)	MGG_03741	XP_003716138.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONf13147	XM_011394812.1 Latest version!	Neurospora crassa OR74A uncharacterized protein (NCU03220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf13148	XM_011394813.1 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU03220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf13149	XM_011394814.1 Latest version!	Neurospora crassa OR74A uncharacterized protein (NCU03220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03220 ( XM_011394812.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393114.1 Neurospora crassa OR74A uncharacterized protein (NCU03220), mRNA.

Price & Availability↑

CloneID	ONf13147
Clone ID Related Accession (Same CDS sequence)	XM_011394812.1
Accession Version	XM_011394812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGGCTC CGTACTGGGG CCAGCTCCCA CCACTAGCTA CAGTGGCTAG GAGTCGGCGC 
ATGTCTGGCG ATGTGACGCC GACAACCCCA TCCGACTCTC GCCAGTCCTT CGATACCCAC 
TCGCAGAACC GCCAAAGACC GGATCGCAAT TCTGTCCAGA CTACAAGGTC GGAAGTTCAA 
ACTGACTCAA ATTCCAGCCC TTTCGCATCG CCGACCACTC CCAGCTTTAT GGGCCACGGA 
CCTGCGCCTC CTCCAGCACC AGTGCCATAC GCTACGGGCA ACCAGTATCC GCCCGACTTA 
CTAGAAAAGC GCCTTCGCCG TCGGAGCCAA AATCAAGACT ATGACTTAGA CTATGCACTC 
GATACTGGCC CACCGCCGCC TGCCGTCCCG GAGGCTCCCA CAGGACCTCC CATGAGTTAC 
AGATATCCCG CTGCCATGGC TGAACAACAA AATTCGTACT TGAATTCCTC TCCGGGATCA 
TCCCCCTTGA CCCAAGACGT GGGCTCAGCT GAATTTCCCC ACAATCAAAC CTCGGCCGGT 
GAACAGCAAG TATCTCGGGA CACGAGTGAT GCGAGCACGA GCTGGCGACC ACAGCGTATC 
GTTAACGGAT CGACGCGTTC GGCAAACATG CCACCATCTG CGGGAGCAAA CAGCCACCGA 
CGCAGGGTTT CTGGTCTTGA CCAGCCTCTA CAGAGGAGTA CTAGGCGCTC GTCAGCGAGC 
CAGCCAGCAA CCCGACAGAG AATCATAGCA AAAGAAGAAT CAGGGGAAGG ACCTACTCCA 
CAAGCCGAAG CCACCGACGG CCCAACACGA AAAGGTGCGC CAGAGAACTC GGCATATGAA 
CAGACCGAAG TAAAGACGAT ATCGCCGCAA GTTCCTGTCC AGATTCAACA GGTCGCGCTC 
GCTTTTCAAC GTCAGGATCA ACCCTCACAG ACTATGCCGG TAAGTTCGAC GACTTCAACC 
AGACCTGACC AAGGTTCGGC GGCTGCAAAG GGACCTCTCT CGCAGAATCC TCCCGAAGAA 
GGGCGAACGT ATGGAGCAAA CGGTGGGCGA GTTGTTAGAA CTCGGGTGCC TGACCCACGG 
ATTCCAATGA ACGCGGCCGT CAGGACTTCT AATCTGCCAC AGCGCAACCT CAGTTTCCGC 
GAGAGAGCAG CTAATCAGGA GGCCAAGCTG CCCAATGATA TCAATGAAGC CTTGGGCCCC 
ATGCCCGCAC CAAGGAAGAT GGTGAAGAAG CCGGTAGCAG AGCCATGGCC AAGGCAAAGC 
CAAGAAAAGC AGGAGGCCCT GGAGTCAGCA GAGGTTCGAG ATCATCAACC AGTTGAGGAT 
ACAGTCGAAA GGGCTGTCAA GGATGACCAA AGTCAGCAGT TCGGCAATAT CACTATTGGA 
GCGAGCGCAG AGCCAGTCAG AGTAGCGAGC CAGAGAAAGG CGGACAAAAT GCTTGGTCGT 
GCCCCCGTCC AGCAACCCCG GCAGTATACA ACACCTACGG AGCGACAAAT CCAAGTTCAG 
ACCGAGCCCG ACCCCCAACC TCCCGTCGAA AGTCCCATCA TTCCTGCCGA AACCCCACAG 
GCTACAACCG AAAGGATGCC CATGCGCGGG CCTAACCATC CTCACCCACC TAACTACCAG 
CACCGACACC AGCACCAGGC ACGAAGAGAT GTTAGACGTG ACGACCAAGG GAGAGATCTT 
AGACGCGACC GCGATGATGC TGAGCCTGGC CATCATATCT CTAATATTAT GTACCATGCG 
CGGGACGACC TCGAACCCGG GCAGGGCAAG TTTCAACCAA CAAAATACCT AGAAGAGTGG 
AAGAATGGCA CAGTCGGTAC TCTTGGCGGC CCGCTTCTTG ACCTGGGGAA TGTATCGCCT 
GTAGGAGATA AGGGCGCACC CCAGTTTCGG AGACGAGGTA CCGGGGGACT GCGGCCAAGG 
AGAGGTGAAG CTTTTGATGG GGAGTATGAT GACACTTATG CTCCCACGAG ATTCAAGCCG 
CCACTCTACC TCAAATGTGG CCCTCTTCTA CGGTTTTGCG GTATCCGGCA TGACCGTGTG 
GCCACCCGCT CCCCCAAACC CGGGGCTGTG TTAGAGAAAG AGATATGGCG CGGTTCAGTC 
ATGATCGTCA CATCAGACGC CGATTCATCA TACGATATTG CCCCAACACT GAGGCTATTT 
GCTCAACCGG TGGAACTCTT ACCCCCTCCG CCGAAAATTA TACATGGTGA TGAGCCTTTG 
GCAGCAGAGT ATGTTGACCC CATCGCAGGC CATCCTAAAT TAGGACGCAA GGGCGAGACT 
CTCTATGTCA GACCGGTCGA CCATCTGGAC GAAGGCAAGG ATCTTTCCCG GGATGAGACC 
GACAACGGAT TATTCGAAAA GTCGAGAAGC GCGCCAGAGT CTCTTCCGAC CGGCGTAACT 
GACCCGCCCG GCTCGTTCAA CGCCCGAAAG AAACGCGCCA GGATTGATGG TGAGAAAGTA 
GGAAAGTACA AAGACGTCCG AGGTTTCCGA CTTCACGCTG AGCGAGGATA CACTTTCTGG 
CGATTTAATA TCGAAGTCGA ACTCCGCGAA AAACAACAAC GCATCGCTTA TCGGATCAAC 
AGGGGACCGG CGACGGGTTT TTGGGTCCCA GCCAAAGGAC AGGCAATGAA CATAATGTTC 
CACAGCTGCA ACGGTTTTAG CTTGAGCGTC GATCCTAACC AGTTCAGCGG CCCCGACCCT 
ATGTGGCGCG ACGTGCTCAA CACTCACCAG AGCCAACCGT TCCATGTGAT GATCGGTGGT 
GGTGACCAAA TCTACAACGA CAAGTGCATG CAAGAGACAC AGCTCTTCAA GGAATGGCTG 
TTAATCAAGG ACCCATCGCA CAAGCATAAC ATTCCTTTCA GCGCGGAGAT GCAGGACGAG 
CTAGAGCGCT TCTACCTGGA GCGATACGCC ATGTGGTTTT CGCAGGGTTT GTTTGGCATG 
GCAGCCTCAC AAATCCCCAT GGTCAACATG TATGATGACC ACGACATCTT TGACGGCTTT 
GGCTCGTATC CCCATCATTT CATGGAGTCC CCCGTGTTCT CGGGACTTGG CAATGTTGCT 
TTCAAGTACT ATATGCTGTT CCAGCATCAG AGCGTGGTGG ATGAAACAGA GAAATCCGAG 
CCAAGCTGGA TCTTAGGTGA AAAACCGGGC CCGTACATCC AGGAGGTCAG TCGAAGTATG 
TTTATGTTCT TGGGCGCAAA GGTTGCACTA CTGGCGGTTG ACGCACGGAC TGAGCGAACG 
CGAGAGGAAG TCATTAGAGA AAATACATGG AAGAGAATTA TGGACCGGTG TTATGGTGAA 
ATCGAAAATG GGAAGGTAGA GCATCTGCTG GTCCTTCTTG GTGTGCCGGT CGCTTACCCG 
AGGCTGGTCT GGCTGGAAAA TATCTTGACC TCTCGTCTGA TGGATCCTGT CAAGGCTCTT 
GGAAGAGCCG GCCTTTTCAA AAACCTCCTG AACCATTTCG ATGGAGGCGT TGAAGTCCTG 
GATGATTTGG ACGACCACTG GACAGCCAAA AACCATAAGG AGGAGCGGGC AATTGTGATC 
GAAGATCTAC AGGACCTGGC AGCTGACAAG TCCGTCAGAG TTACTATCCT CAGCGGTGAC 
GTCCACTTGG CAGCCATCGG GCAATTTTAC TCGAACCCCA AGCTCAACAT CCCGAAGGAC 
AAGGACTTCA GGTACATGCC AAACGTCATC TCCTCCGCCA TCGTCAACGC TCCGCCTTCA 
GATATGTTGG CCGACGTGCT CAACAAACGT AACAAGGTGC ATCATTTCGA CCGAGACACG 
GACGAAGACA TGATCCCAAT GTTCCAGCAA GGTGTCGATG GCAAGCCACG CAACAACAAG 
CATCTGCTTC CACACCGCAA CTGGTGCTCA ATTCGGCTGT ATAACCCCGG GAACACACCA 
GACTCCACAC CCGGCCAGTC TGTAATTGGC ATCAACGCTT ACGGACTACC TTCCAACGGA 
CCCAGCCTTT TACGTCGCCT CTCACTTTCC AAAACACGAA GCCAATCCGG CCCCACATAC 
CGTGGCCCCG ACAGCGTGCG CGACCGAACC CGCCCGCCCA TCTCCAACGC GTTGCTCCGC 
ACTCTTTCCC GCCGCGGACC AAGCGCAAGC GCTGATCAAG TCGTCCCTGG CGGCCGCGGA 
AGCCCAGCGG CAGCAGGAAA GCCCCCCGGG TTGTTGAAGA GGACCCTCTC GGGCTCCTCC 
ATAAGCGGTA AAGTCGGCGA CTTCTTCCGC CGACGTGACA GCAACAGCTC CCAGCGGCCG 
CGTGTGGGCG ATGAGGGCAT TAACGGGACT TGGGTTCCGG AATCGGACGC TGAACAGGCT 
GTTTACGATG ACTATGCCCA TGTTGGGGAT GAGTATAACA TCCCTAAGAT CCGCGGTGGC 
ATTCGCGATG GCATGTCTGC TCGTGTGGGC TTGCGCGGTG GAGGGGGTGA ATATTATCCT 
ACTCCCCCCG TGCCCGCTGG TACTGGTGGT GACGAGTTTG TTGCCGGTGA CGAGAGTTAC 
TTTACGGCCA AACCGGTCTC TGATCACAAT CGTGGAGTTA GTACCGGTGG CGAGTATCCC 
TCTTCACCAA TGACCGGTTT CAGTAACACC GGCCGGGGTG GTCCCACGGG CATGGACATG 
GGCATATTCC AGTCCCAAGC AACATCCGCC TCCTTACCAG AACGGGACTT TGCACCTTCT 
CAACCCTCAC CTCGTAGTGG CGGCAGTATG TTCCGTTCCA GAACAACAGG CGGTGACACA 
AGAACCATAA AGCGCGGAGA CGCCATACCT CGAATCATGG CCGACGGCGC ACTGGAAGTC 
ACCCTCAACG TGGAGATCAG CCCGCGCGAT CCGGGAGGCA GCACGGTGCC GTACCGCCTG 
CTGGTGCCCA AGTTGTCGTA TGAGTATGAG GGCGAGGATC ATGACCACGA TCACGATCCG 
GAGATGAATG CGATGGTTGG GGAGGAGCGT ACGTCGAGCC CGGGGCAGGT GCAGACGTTA 
GAGTCTAGGC AGCGGGAGGA GACGCGGACG GAGATGGGGA TGGAGATGGG AATGGTGATG 
GGGATGGAGG AGAAGCCTAT CACTAGTGGT GGTGGAGGGC TTAGACGGTT GCTTAGTTTA 
AAAAGGAATG GGCGACGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393114.1
CDS	887..6007
Translation	MSAPYWGQLPPLATVARSRRMSGDVTPTTPSDSRQSFDTHSQNRQRPDRNSVQTTRSEVQTDSNSSPFASPTTPSFMGHGPAPPPAPVPYATGNQYPPDLLEKRLRRRSQNQDYDLDYALDTGPPPPAVPEAPTGPPMSYRYPAAMAEQQNSYLNSSPGSSPLTQDVGSAEFPHNQTSAGEQQVSRDTSDASTSWRPQRIVNGSTRSANMPPSAGANSHRRRVSGLDQPLQRSTRRSSASQPATRQRIIAKEESGEGPTPQAEATDGPTRKGAPENSAYEQTEVKTISPQVPVQIQQVALAFQRQDQPSQTMPVSSTTSTRPDQGSAAAKGPLSQNPPEEGRTYGANGGRVVRTRVPDPRIPMNAAVRTSNLPQRNLSFRERAANQEAKLPNDINEALGPMPAPRKMVKKPVAEPWPRQSQEKQEALESAEVRDHQPVEDTVERAVKDDQSQQFGNITIGASAEPVRVASQRKADKMLGRAPVQQPRQYTTPTERQIQVQTEPDPQPPVESPIIPAETPQATTERMPMRGPNHPHPPNYQHRHQHQARRDVRRDDQGRDLRRDRDDAEPGHHISNIMYHARDDLEPGQGKFQPTKYLEEWKNGTVGTLGGPLLDLGNVSPVGDKGAPQFRRRGTGGLRPRRGEAFDGEYDDTYAPTRFKPPLYLKCGPLLRFCGIRHDRVATRSPKPGAVLEKEIWRGSVMIVTSDADSSYDIAPTLRLFAQPVELLPPPPKIIHGDEPLAAEYVDPIAGHPKLGRKGETLYVRPVDHLDEGKDLSRDETDNGLFEKSRSAPESLPTGVTDPPGSFNARKKRARIDGEKVGKYKDVRGFRLHAERGYTFWRFNIEVELREKQQRIAYRINRGPATGFWVPAKGQAMNIMFHSCNGFSLSVDPNQFSGPDPMWRDVLNTHQSQPFHVMIGGGDQIYNDKCMQETQLFKEWLLIKDPSHKHNIPFSAEMQDELERFYLERYAMWFSQGLFGMAASQIPMVNMYDDHDIFDGFGSYPHHFMESPVFSGLGNVAFKYYMLFQHQSVVDETEKSEPSWILGEKPGPYIQEVSRSMFMFLGAKVALLAVDARTERTREEVIRENTWKRIMDRCYGEIENGKVEHLLVLLGVPVAYPRLVWLENILTSRLMDPVKALGRAGLFKNLLNHFDGGVEVLDDLDDHWTAKNHKEERAIVIEDLQDLAADKSVRVTILSGDVHLAAIGQFYSNPKLNIPKDKDFRYMPNVISSAIVNAPPSDMLADVLNKRNKVHHFDRDTDEDMIPMFQQGVDGKPRNNKHLLPHRNWCSIRLYNPGNTPDSTPGQSVIGINAYGLPSNGPSLLRRLSLSKTRSQSGPTYRGPDSVRDRTRPPISNALLRTLSRRGPSASADQVVPGGRGSPAAAGKPPGLLKRTLSGSSISGKVGDFFRRRDSNSSQRPRVGDEGINGTWVPESDAEQAVYDDYAHVGDEYNIPKIRGGIRDGMSARVGLRGGGGEYYPTPPVPAGTGGDEFVAGDESYFTAKPVSDHNRGVSTGGEYPSSPMTGFSNTGRGGPTGMDMGIFQSQATSASLPERDFAPSQPSPRSGGSMFRSRTTGGDTRTIKRGDAIPRIMADGALEVTLNVEISPRDPGGSTVPYRLLVPKLSYEYEGEDHDHDHDPEMNAMVGEERTSSPGQVQTLESRQREETRTEMGMEMGMVMGMEEKPITSGGGGLRRLLSLKRNGRR

Target ORF information:

RefSeq Version	XM_011394812.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A uncharacterized protein (NCU03220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394812.1

ORF Insert Sequence:

ATGTCGGCTC CGTACTGGGG CCAGCTCCCA CCACTAGCTA CAGTGGCTAG GAGTCGGCGC 
ATGTCTGGCG ATGTGACGCC GACAACCCCA TCCGACTCTC GCCAGTCCTT CGATACCCAC 
TCGCAGAACC GCCAAAGACC GGATCGCAAT TCTGTCCAGA CTACAAGGTC GGAAGTTCAA 
ACTGACTCAA ATTCCAGCCC TTTCGCATCG CCGACCACTC CCAGCTTTAT GGGCCACGGA 
CCTGCGCCTC CTCCAGCACC AGTGCCATAC GCTACGGGCA ACCAGTATCC GCCCGACTTA 
CTAGAAAAGC GCCTTCGCCG TCGGAGCCAA AATCAAGACT ATGACTTAGA CTATGCACTC 
GATACTGGCC CACCGCCGCC TGCCGTCCCG GAGGCTCCCA CAGGACCTCC CATGAGTTAC 
AGATATCCCG CTGCCATGGC TGAACAACAA AATTCGTACT TGAATTCCTC TCCGGGATCA 
TCCCCCTTGA CCCAAGACGT GGGCTCAGCT GAATTTCCCC ACAATCAAAC CTCGGCCGGT 
GAACAGCAAG TATCTCGGGA CACGAGTGAT GCGAGCACGA GCTGGCGACC ACAGCGTATC 
GTTAACGGAT CGACGCGTTC GGCAAACATG CCACCATCTG CGGGAGCAAA CAGCCACCGA 
CGCAGGGTTT CTGGTCTTGA CCAGCCTCTA CAGAGGAGTA CTAGGCGCTC GTCAGCGAGC 
CAGCCAGCAA CCCGACAGAG AATCATAGCA AAAGAAGAAT CAGGGGAAGG ACCTACTCCA 
CAAGCCGAAG CCACCGACGG CCCAACACGA AAAGGTGCGC CAGAGAACTC GGCATATGAA 
CAGACCGAAG TAAAGACGAT ATCGCCGCAA GTTCCTGTCC AGATTCAACA GGTCGCGCTC 
GCTTTTCAAC GTCAGGATCA ACCCTCACAG ACTATGCCGG TAAGTTCGAC GACTTCAACC 
AGACCTGACC AAGGTTCGGC GGCTGCAAAG GGACCTCTCT CGCAGAATCC TCCCGAAGAA 
GGGCGAACGT ATGGAGCAAA CGGTGGGCGA GTTGTTAGAA CTCGGGTGCC TGACCCACGG 
ATTCCAATGA ACGCGGCCGT CAGGACTTCT AATCTGCCAC AGCGCAACCT CAGTTTCCGC 
GAGAGAGCAG CTAATCAGGA GGCCAAGCTG CCCAATGATA TCAATGAAGC CTTGGGCCCC 
ATGCCCGCAC CAAGGAAGAT GGTGAAGAAG CCGGTAGCAG AGCCATGGCC AAGGCAAAGC 
CAAGAAAAGC AGGAGGCCCT GGAGTCAGCA GAGGTTCGAG ATCATCAACC AGTTGAGGAT 
ACAGTCGAAA GGGCTGTCAA GGATGACCAA AGTCAGCAGT TCGGCAATAT CACTATTGGA 
GCGAGCGCAG AGCCAGTCAG AGTAGCGAGC CAGAGAAAGG CGGACAAAAT GCTTGGTCGT 
GCCCCCGTCC AGCAACCCCG GCAGTATACA ACACCTACGG AGCGACAAAT CCAAGTTCAG 
ACCGAGCCCG ACCCCCAACC TCCCGTCGAA AGTCCCATCA TTCCTGCCGA AACCCCACAG 
GCTACAACCG AAAGGATGCC CATGCGCGGG CCTAACCATC CTCACCCACC TAACTACCAG 
CACCGACACC AGCACCAGGC ACGAAGAGAT GTTAGACGTG ACGACCAAGG GAGAGATCTT 
AGACGCGACC GCGATGATGC TGAGCCTGGC CATCATATCT CTAATATTAT GTACCATGCG 
CGGGACGACC TCGAACCCGG GCAGGGCAAG TTTCAACCAA CAAAATACCT AGAAGAGTGG 
AAGAATGGCA CAGTCGGTAC TCTTGGCGGC CCGCTTCTTG ACCTGGGGAA TGTATCGCCT 
GTAGGAGATA AGGGCGCACC CCAGTTTCGG AGACGAGGTA CCGGGGGACT GCGGCCAAGG 
AGAGGTGAAG CTTTTGATGG GGAGTATGAT GACACTTATG CTCCCACGAG ATTCAAGCCG 
CCACTCTACC TCAAATGTGG CCCTCTTCTA CGGTTTTGCG GTATCCGGCA TGACCGTGTG 
GCCACCCGCT CCCCCAAACC CGGGGCTGTG TTAGAGAAAG AGATATGGCG CGGTTCAGTC 
ATGATCGTCA CATCAGACGC CGATTCATCA TACGATATTG CCCCAACACT GAGGCTATTT 
GCTCAACCGG TGGAACTCTT ACCCCCTCCG CCGAAAATTA TACATGGTGA TGAGCCTTTG 
GCAGCAGAGT ATGTTGACCC CATCGCAGGC CATCCTAAAT TAGGACGCAA GGGCGAGACT 
CTCTATGTCA GACCGGTCGA CCATCTGGAC GAAGGCAAGG ATCTTTCCCG GGATGAGACC 
GACAACGGAT TATTCGAAAA GTCGAGAAGC GCGCCAGAGT CTCTTCCGAC CGGCGTAACT 
GACCCGCCCG GCTCGTTCAA CGCCCGAAAG AAACGCGCCA GGATTGATGG TGAGAAAGTA 
GGAAAGTACA AAGACGTCCG AGGTTTCCGA CTTCACGCTG AGCGAGGATA CACTTTCTGG 
CGATTTAATA TCGAAGTCGA ACTCCGCGAA AAACAACAAC GCATCGCTTA TCGGATCAAC 
AGGGGACCGG CGACGGGTTT TTGGGTCCCA GCCAAAGGAC AGGCAATGAA CATAATGTTC 
CACAGCTGCA ACGGTTTTAG CTTGAGCGTC GATCCTAACC AGTTCAGCGG CCCCGACCCT 
ATGTGGCGCG ACGTGCTCAA CACTCACCAG AGCCAACCGT TCCATGTGAT GATCGGTGGT 
GGTGACCAAA TCTACAACGA CAAGTGCATG CAAGAGACAC AGCTCTTCAA GGAATGGCTG 
TTAATCAAGG ACCCATCGCA CAAGCATAAC ATTCCTTTCA GCGCGGAGAT GCAGGACGAG 
CTAGAGCGCT TCTACCTGGA GCGATACGCC ATGTGGTTTT CGCAGGGTTT GTTTGGCATG 
GCAGCCTCAC AAATCCCCAT GGTCAACATG TATGATGACC ACGACATCTT TGACGGCTTT 
GGCTCGTATC CCCATCATTT CATGGAGTCC CCCGTGTTCT CGGGACTTGG CAATGTTGCT 
TTCAAGTACT ATATGCTGTT CCAGCATCAG AGCGTGGTGG ATGAAACAGA GAAATCCGAG 
CCAAGCTGGA TCTTAGGTGA AAAACCGGGC CCGTACATCC AGGAGGTCAG TCGAAGTATG 
TTTATGTTCT TGGGCGCAAA GGTTGCACTA CTGGCGGTTG ACGCACGGAC TGAGCGAACG 
CGAGAGGAAG TCATTAGAGA AAATACATGG AAGAGAATTA TGGACCGGTG TTATGGTGAA 
ATCGAAAATG GGAAGGTAGA GCATCTGCTG GTCCTTCTTG GTGTGCCGGT CGCTTACCCG 
AGGCTGGTCT GGCTGGAAAA TATCTTGACC TCTCGTCTGA TGGATCCTGT CAAGGCTCTT 
GGAAGAGCCG GCCTTTTCAA AAACCTCCTG AACCATTTCG ATGGAGGCGT TGAAGTCCTG 
GATGATTTGG ACGACCACTG GACAGCCAAA AACCATAAGG AGGAGCGGGC AATTGTGATC 
GAAGATCTAC AGGACCTGGC AGCTGACAAG TCCGTCAGAG TTACTATCCT CAGCGGTGAC 
GTCCACTTGG CAGCCATCGG GCAATTTTAC TCGAACCCCA AGCTCAACAT CCCGAAGGAC 
AAGGACTTCA GGTACATGCC AAACGTCATC TCCTCCGCCA TCGTCAACGC TCCGCCTTCA 
GATATGTTGG CCGACGTGCT CAACAAACGT AACAAGGTGC ATCATTTCGA CCGAGACACG 
GACGAAGACA TGATCCCAAT GTTCCAGCAA GGTGTCGATG GCAAGCCACG CAACAACAAG 
CATCTGCTTC CACACCGCAA CTGGTGCTCA ATTCGGCTGT ATAACCCCGG GAACACACCA 
GACTCCACAC CCGGCCAGTC TGTAATTGGC ATCAACGCTT ACGGACTACC TTCCAACGGA 
CCCAGCCTTT TACGTCGCCT CTCACTTTCC AAAACACGAA GCCAATCCGG CCCCACATAC 
CGTGGCCCCG ACAGCGTGCG CGACCGAACC CGCCCGCCCA TCTCCAACGC GTTGCTCCGC 
ACTCTTTCCC GCCGCGGACC AAGCGCAAGC GCTGATCAAG TCGTCCCTGG CGGCCGCGGA 
AGCCCAGCGG CAGCAGGAAA GCCCCCCGGG TTGTTGAAGA GGACCCTCTC GGGCTCCTCC 
ATAAGCGGTA AAGTCGGCGA CTTCTTCCGC CGACGTGACA GCAACAGCTC CCAGCGGCCG 
CGTGTGGGCG ATGAGGGCAT TAACGGGACT TGGGTTCCGG AATCGGACGC TGAACAGGCT 
GTTTACGATG ACTATGCCCA TGTTGGGGAT GAGTATAACA TCCCTAAGAT CCGCGGTGGC 
ATTCGCGATG GCATGTCTGC TCGTGTGGGC TTGCGCGGTG GAGGGGGTGA ATATTATCCT 
ACTCCCCCCG TGCCCGCTGG TACTGGTGGT GACGAGTTTG TTGCCGGTGA CGAGAGTTAC 
TTTACGGCCA AACCGGTCTC TGATCACAAT CGTGGAGTTA GTACCGGTGG CGAGTATCCC 
TCTTCACCAA TGACCGGTTT CAGTAACACC GGCCGGGGTG GTCCCACGGG CATGGACATG 
GGCATATTCC AGTCCCAAGC AACATCCGCC TCCTTACCAG AACGGGACTT TGCACCTTCT 
CAACCCTCAC CTCGTAGTGG CGGCAGTATG TTCCGTTCCA GAACAACAGG CGGTGACACA 
AGAACCATAA AGCGCGGAGA CGCCATACCT CGAATCATGG CCGACGGCGC ACTGGAAGTC 
ACCCTCAACG TGGAGATCAG CCCGCGCGAT CCGGGAGGCA GCACGGTGCC GTACCGCCTG 
CTGGTGCCCA AGTTGTCGTA TGAGTATGAG GGCGAGGATC ATGACCACGA TCACGATCCG 
GAGATGAATG CGATGGTTGG GGAGGAGCGT ACGTCGAGCC CGGGGCAGGT GCAGACGTTA 
GAGTCTAGGC AGCGGGAGGA GACGCGGACG GAGATGGGGA TGGAGATGGG AATGGTGATG 
GGGATGGAGG AGAAGCCTAT CACTAGTGGT GGTGGAGGGC TTAGACGGTT GCTTAGTTTA 
AAAAGGAATG GGCGACGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03220 ( XM_011394813.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393115.1 Neurospora crassa OR74A hypothetical protein (NCU03220), mRNA.

Price & Availability↑

CloneID	ONf13148
Clone ID Related Accession (Same CDS sequence)	XM_011394813.1
Accession Version	XM_011394813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGGCTC CGTACTGGGG CCAGCTCCCA CCACTAGCTA CAGTGGCTAG GAGTCGGCGC 
ATGTCTGGCG ATGTGACGCC GACAACCCCA TCCGACTCTC GCCAGTCCTT CGATACCCAC 
TCGCAGAACC GCCAAAGACC GGATCGCAAT TCTGTCCAGA CTACAAGGTC GGAAGTTCAA 
ACTGACTCAA ATTCCAGCCC TTTCGCATCG CCGACCACTC CCAGCTTTAT GGGCCACGGA 
CCTGCGCCTC CTCCAGCACC AGTGCCATAC GCTACGGGCA ACCAGTATCC GCCCGACTTA 
CTAGAAAAGC GCCTTCGCCG TCGGAGCCAA AATCAAGACT ATGACTTAGA CTATGCACTC 
GATACTGGCC CACCGCCGCC TGCCGTCCCG GAGGCTCCCA CAGGACCTCC CATGAGTTAC 
AGATATCCCG CTGCCATGGC TGAACAACAA AATTCGTACT TGAATTCCTC TCCGGGATCA 
TCCCCCTTGA CCCAAGACGT GGGCTCAGCT GAATTTCCCC ACAATCAAAC CTCGGCCGGT 
GAACAGCAAG TATCTCGGGA CACGAGTGAT GCGAGCACGA GCTGGCGACC ACAGCGTATC 
GTTAACGGAT CGACGCGTTC GGCAAACATG CCACCATCTG CGGGAGCAAA CAGCCACCGA 
CGCAGGGTTT CTGGTCTTGA CCAGCCTCTA CAGAGGAGTA CTAGGCGCTC GTCAGCGAGC 
CAGCCAGCAA CCCGACAGAG AATGTTCGCA GACGACCGCT CACCACTTCA GAGGCTCCAG 
CTGACCCTGG ACAGCATAGC AAAAGAAGAA TCAGGGGAAG GACCTACTCC ACAAGCCGAA 
GCCACCGACG GCCCAACACG AAAAGGTGCG CCAGAGAACT CGGCATATGA ACAGACCGAA 
GTAAAGACGA TATCGCCGCA AGTTCCTGTC CAGATTCAAC AGGTCGCGCT CGCTTTTCAA 
CGTCAGGATC AACCCTCACA GACTATGCCG GTAAGTTCGA CGACTTCAAC CAGACCTGAC 
CAAGGTTCGG CGGCTGCAAA GGGACCTCTC TCGCAGAATC CTCCCGAAGA AGGGCGAACG 
TATGGAGCAA ACGGTGGGCG AGTTGTTAGA ACTCGGGTGC CTGACCCACG GATTCCAATG 
AACGCGGCCG TCAGGACTTC TAATCTGCCA CAGCGCAACC TCAGTTTCCG CGAGAGAGCA 
GCTAATCAGG AGGCCAAGCT GCCCAATGAT ATCAATGAAG CCTTGGGCCC CATGCCCGCA 
CCAAGGAAGA TGGTGAAGAA GCCGGTAGCA GAGCCATGGC CAAGGCAAAG CCAAGAAAAG 
CAGGAGGCCC TGGAGTCAGC AGAGGTTCGA GATCATCAAC CAGTTGAGGA TACAGTCGAA 
AGGGCTGTCA AGGATGACCA AAGTCAGCAG TTCGGCAATA TCACTATTGG AGCGAGCGCA 
GAGCCAGTCA GAGTAGCGAG CCAGAGAAAG GCGGACAAAA TGCTTGGTCG TGCCCCCGTC 
CAGCAACCCC GGCAGTATAC AACACCTACG GAGCGACAAA TCCAAGTTCA GACCGAGCCC 
GACCCCCAAC CTCCCGTCGA AAGTCCCATC ATTCCTGCCG AAACCCCACA GGCTACAACC 
GAAAGGATGC CCATGCGCGG GCCTAACCAT CCTCACCCAC CTAACTACCA GCACCGACAC 
CAGCACCAGG CACGAAGAGA TGTTAGACGT GACGACCAAG GGAGAGATCT TAGACGCGAC 
CGCGATGATG CTGAGCCTGG CCATCATATC TCTAATATTA TGTACCATGC GCGGGACGAC 
CTCGAACCCG GGCAGGGCAA GTTTCAACCA ACAAAATACC TAGAAGAGTG GAAGAATGGC 
ACAGTCGGTA CTCTTGGCGG CCCGCTTCTT GACCTGGGGA ATGTATCGCC TGTAGGAGAT 
AAGGGCGCAC CCCAGTTTCG GAGACGAGGT ACCGGGGGAC TGCGGCCAAG GAGAGGTGAA 
GCTTTTGATG GGGAGTATGA TGACACTTAT GCTCCCACGA GATTCAAGCC GCCACTCTAC 
CTCAAATGTG GCCCTCTTCT ACGGTTTTGC GGTATCCGGC ATGACCGTGT GGCCACCCGC 
TCCCCCAAAC CCGGGGCTGT GTTAGAGAAA GAGATATGGC GCGGTTCAGT CATGATCGTC 
ACATCAGACG CCGATTCATC ATACGATATT GCCCCAACAC TGAGGCTATT TGCTCAACCG 
GTGGAACTCT TACCCCCTCC GCCGAAAATT ATACATGGTG ATGAGCCTTT GGCAGCAGAG 
TATGTTGACC CCATCGCAGG CCATCCTAAA TTAGGACGCA AGGGCGAGAC TCTCTATGTC 
AGACCGGTCG ACCATCTGGA CGAAGGCAAG GATCTTTCCC GGGATGAGAC CGACAACGGA 
TTATTCGAAA AGTCGAGAAG CGCGCCAGAG TCTCTTCCGA CCGGCGTAAC TGACCCGCCC 
GGCTCGTTCA ACGCCCGAAA GAAACGCGCC AGGATTGATG GTGAGAAAGT AGGAAAGTAC 
AAAGACGTCC GAGGTTTCCG ACTTCACGCT GAGCGAGGAT ACACTTTCTG GCGATTTAAT 
ATCGAAGTCG AACTCCGCGA AAAACAACAA CGCATCGCTT ATCGGATCAA CAGGGGACCG 
GCGACGGGTT TTTGGGTCCC AGCCAAAGGA CAGGCAATGA ACATAATGTT CCACAGCTGC 
AACGGTTTTA GCTTGAGCGT CGATCCTAAC CAGTTCAGCG GCCCCGACCC TATGTGGCGC 
GACGTGCTCA ACACTCACCA GAGCCAACCG TTCCATGTGA TGATCGGTGG TGGTGACCAA 
ATCTACAACG ACAAGTGCAT GCAAGAGACA CAGCTCTTCA AGGAATGGCT GTTAATCAAG 
GACCCATCGC ACAAGCATAA CATTCCTTTC AGCGCGGAGA TGCAGGACGA GCTAGAGCGC 
TTCTACCTGG AGCGATACGC CATGTGGTTT TCGCAGGGTT TGTTTGGCAT GGCAGCCTCA 
CAAATCCCCA TGGTCAACAT GTATGATGAC CACGACATCT TTGACGGCTT TGGCTCGTAT 
CCCCATCATT TCATGGAGTC CCCCGTGTTC TCGGGACTTG GCAATGTTGC TTTCAAGTAC 
TATATGCTGT TCCAGCATCA GAGCGTGGTG GATGAAACAG AGAAATCCGA GCCAAGCTGG 
ATCTTAGGTG AAAAACCGGG CCCGTACATC CAGGAGGTCA GTCGAAGTAT GTTTATGTTC 
TTGGGCGCAA AGGTTGCACT ACTGGCGGTT GACGCACGGA CTGAGCGAAC GCGAGAGGAA 
GTCATTAGAG AAAATACATG GAAGAGAATT ATGGACCGGT GTTATGGTGA AATCGAAAAT 
GGGAAGGTAG AGCATCTGCT GGTCCTTCTT GGTGTGCCGG TCGCTTACCC GAGGCTGGTC 
TGGCTGGAAA ATATCTTGAC CTCTCGTCTG ATGGATCCTG TCAAGGCTCT TGGAAGAGCC 
GGCCTTTTCA AAAACCTCCT GAACCATTTC GATGGAGGCG TTGAAGTCCT GGATGATTTG 
GACGACCACT GGACAGCCAA AAACCATAAG GAGGAGCGGG CAATTGTGAT CGAAGATCTA 
CAGGACCTGG CAGCTGACAA GTCCGTCAGA GTTACTATCC TCAGCGGTGA CGTCCACTTG 
GCAGCCATCG GGCAATTTTA CTCGAACCCC AAGCTCAACA TCCCGAAGGA CAAGGACTTC 
AGGTACATGC CAAACGTCAT CTCCTCCGCC ATCGTCAACG CTCCGCCTTC AGATATGTTG 
GCCGACGTGC TCAACAAACG TAACAAGGTG CATCATTTCG ACCGAGACAC GGACGAAGAC 
ATGATCCCAA TGTTCCAGCA AGGTGTCGAT GGCAAGCCAC GCAACAACAA GCATCTGCTT 
CCACACCGCA ACTGGTGCTC AATTCGGCTG TATAACCCCG GGAACACACC AGACTCCACA 
CCCGGCCAGT CTGTAATTGG CATCAACGCT TACGGACTAC CTTCCAACGG ACCCAGCCTT 
TTACGTCGCC TCTCACTTTC CAAAACACGA AGCCAATCCG GCCCCACATA CCGTGGCCCC 
GACAGCGTGC GCGACCGAAC CCGCCCGCCC ATCTCCAACG CGTTGCTCCG CACTCTTTCC 
CGCCGCGGAC CAAGCGCAAG CGCTGATCAA GTCGTCCCTG GCGGCCGCGG AAGCCCAGCG 
GCAGCAGGAA AGCCCCCCGG GTTGTTGAAG AGGACCCTCT CGGGCTCCTC CATAAGCGGT 
AAAGTCGGCG ACTTCTTCCG CCGACGTGAC AGCAACAGCT CCCAGCGGCC GCGTGTGGGC 
GATGAGGGCA TTAACGGGAC TTGGGTTCCG GAATCGGACG CTGAACAGGC TGTTTACGAT 
GACTATGCCC ATGTTGGGGA TGAGTATAAC ATCCCTAAGA TCCGCGGTGG CATTCGCGAT 
GGCATGTCTG CTCGTGTGGG CTTGCGCGGT GGAGGGGGTG AATATTATCC TACTCCCCCC 
GTGCCCGCTG GTACTGGTGG TGACGAGTTT GTTGCCGGTG ACGAGAGTTA CTTTACGGCC 
AAACCGGTCT CTGATCACAA TCGTGGAGTT AGTACCGGTG GCGAGTATCC CTCTTCACCA 
ATGACCGGTT TCAGTAACAC CGGCCGGGGT GGTCCCACGG GCATGGACAT GGGCATATTC 
CAGTCCCAAG CAACATCCGC CTCCTTACCA GAACGGGACT TTGCACCTTC TCAACCCTCA 
CCTCGTAGTG GCGGCAGTAT GTTCCGTTCC AGAACAACAG GCGGTGACAC AAGAACCATA 
AAGCGCGGAG ACGCCATACC TCGAATCATG GCCGACGGCG CACTGGAAGT CACCCTCAAC 
GTGGAGATCA GCCCGCGCGA TCCGGGAGGC AGCACGGTGC CGTACCGCCT GCTGGTGCCC 
AAGTTGTCGT ATGAGTATGA GGGCGAGGAT CATGACCACG ATCACGATCC GGAGATGAAT 
GCGATGGTTG GGGAGGAGCG TACGTCGAGC CCGGGGCAGG TGCAGACGTT AGAGTCTAGG 
CAGCGGGAGG AGACGCGGAC GGAGATGGGG ATGGAGATGG GAATGGTGAT GGGGATGGAG 
GAGAAGCCTA TCACTAGTGG TGGTGGAGGG CTTAGACGGT TGCTTAGTTT AAAAAGGAAT 
GGGCGACGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393115.1
CDS	908..6079
Translation	MSAPYWGQLPPLATVARSRRMSGDVTPTTPSDSRQSFDTHSQNRQRPDRNSVQTTRSEVQTDSNSSPFASPTTPSFMGHGPAPPPAPVPYATGNQYPPDLLEKRLRRRSQNQDYDLDYALDTGPPPPAVPEAPTGPPMSYRYPAAMAEQQNSYLNSSPGSSPLTQDVGSAEFPHNQTSAGEQQVSRDTSDASTSWRPQRIVNGSTRSANMPPSAGANSHRRRVSGLDQPLQRSTRRSSASQPATRQRMFADDRSPLQRLQLTLDSIAKEESGEGPTPQAEATDGPTRKGAPENSAYEQTEVKTISPQVPVQIQQVALAFQRQDQPSQTMPVSSTTSTRPDQGSAAAKGPLSQNPPEEGRTYGANGGRVVRTRVPDPRIPMNAAVRTSNLPQRNLSFRERAANQEAKLPNDINEALGPMPAPRKMVKKPVAEPWPRQSQEKQEALESAEVRDHQPVEDTVERAVKDDQSQQFGNITIGASAEPVRVASQRKADKMLGRAPVQQPRQYTTPTERQIQVQTEPDPQPPVESPIIPAETPQATTERMPMRGPNHPHPPNYQHRHQHQARRDVRRDDQGRDLRRDRDDAEPGHHISNIMYHARDDLEPGQGKFQPTKYLEEWKNGTVGTLGGPLLDLGNVSPVGDKGAPQFRRRGTGGLRPRRGEAFDGEYDDTYAPTRFKPPLYLKCGPLLRFCGIRHDRVATRSPKPGAVLEKEIWRGSVMIVTSDADSSYDIAPTLRLFAQPVELLPPPPKIIHGDEPLAAEYVDPIAGHPKLGRKGETLYVRPVDHLDEGKDLSRDETDNGLFEKSRSAPESLPTGVTDPPGSFNARKKRARIDGEKVGKYKDVRGFRLHAERGYTFWRFNIEVELREKQQRIAYRINRGPATGFWVPAKGQAMNIMFHSCNGFSLSVDPNQFSGPDPMWRDVLNTHQSQPFHVMIGGGDQIYNDKCMQETQLFKEWLLIKDPSHKHNIPFSAEMQDELERFYLERYAMWFSQGLFGMAASQIPMVNMYDDHDIFDGFGSYPHHFMESPVFSGLGNVAFKYYMLFQHQSVVDETEKSEPSWILGEKPGPYIQEVSRSMFMFLGAKVALLAVDARTERTREEVIRENTWKRIMDRCYGEIENGKVEHLLVLLGVPVAYPRLVWLENILTSRLMDPVKALGRAGLFKNLLNHFDGGVEVLDDLDDHWTAKNHKEERAIVIEDLQDLAADKSVRVTILSGDVHLAAIGQFYSNPKLNIPKDKDFRYMPNVISSAIVNAPPSDMLADVLNKRNKVHHFDRDTDEDMIPMFQQGVDGKPRNNKHLLPHRNWCSIRLYNPGNTPDSTPGQSVIGINAYGLPSNGPSLLRRLSLSKTRSQSGPTYRGPDSVRDRTRPPISNALLRTLSRRGPSASADQVVPGGRGSPAAAGKPPGLLKRTLSGSSISGKVGDFFRRRDSNSSQRPRVGDEGINGTWVPESDAEQAVYDDYAHVGDEYNIPKIRGGIRDGMSARVGLRGGGGEYYPTPPVPAGTGGDEFVAGDESYFTAKPVSDHNRGVSTGGEYPSSPMTGFSNTGRGGPTGMDMGIFQSQATSASLPERDFAPSQPSPRSGGSMFRSRTTGGDTRTIKRGDAIPRIMADGALEVTLNVEISPRDPGGSTVPYRLLVPKLSYEYEGEDHDHDHDPEMNAMVGEERTSSPGQVQTLESRQREETRTEMGMEMGMVMGMEEKPITSGGGGLRRLLSLKRNGRR

Target ORF information:

RefSeq Version	XM_011394813.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU03220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394813.1

ORF Insert Sequence:

ATGTCGGCTC CGTACTGGGG CCAGCTCCCA CCACTAGCTA CAGTGGCTAG GAGTCGGCGC 
ATGTCTGGCG ATGTGACGCC GACAACCCCA TCCGACTCTC GCCAGTCCTT CGATACCCAC 
TCGCAGAACC GCCAAAGACC GGATCGCAAT TCTGTCCAGA CTACAAGGTC GGAAGTTCAA 
ACTGACTCAA ATTCCAGCCC TTTCGCATCG CCGACCACTC CCAGCTTTAT GGGCCACGGA 
CCTGCGCCTC CTCCAGCACC AGTGCCATAC GCTACGGGCA ACCAGTATCC GCCCGACTTA 
CTAGAAAAGC GCCTTCGCCG TCGGAGCCAA AATCAAGACT ATGACTTAGA CTATGCACTC 
GATACTGGCC CACCGCCGCC TGCCGTCCCG GAGGCTCCCA CAGGACCTCC CATGAGTTAC 
AGATATCCCG CTGCCATGGC TGAACAACAA AATTCGTACT TGAATTCCTC TCCGGGATCA 
TCCCCCTTGA CCCAAGACGT GGGCTCAGCT GAATTTCCCC ACAATCAAAC CTCGGCCGGT 
GAACAGCAAG TATCTCGGGA CACGAGTGAT GCGAGCACGA GCTGGCGACC ACAGCGTATC 
GTTAACGGAT CGACGCGTTC GGCAAACATG CCACCATCTG CGGGAGCAAA CAGCCACCGA 
CGCAGGGTTT CTGGTCTTGA CCAGCCTCTA CAGAGGAGTA CTAGGCGCTC GTCAGCGAGC 
CAGCCAGCAA CCCGACAGAG AATGTTCGCA GACGACCGCT CACCACTTCA GAGGCTCCAG 
CTGACCCTGG ACAGCATAGC AAAAGAAGAA TCAGGGGAAG GACCTACTCC ACAAGCCGAA 
GCCACCGACG GCCCAACACG AAAAGGTGCG CCAGAGAACT CGGCATATGA ACAGACCGAA 
GTAAAGACGA TATCGCCGCA AGTTCCTGTC CAGATTCAAC AGGTCGCGCT CGCTTTTCAA 
CGTCAGGATC AACCCTCACA GACTATGCCG GTAAGTTCGA CGACTTCAAC CAGACCTGAC 
CAAGGTTCGG CGGCTGCAAA GGGACCTCTC TCGCAGAATC CTCCCGAAGA AGGGCGAACG 
TATGGAGCAA ACGGTGGGCG AGTTGTTAGA ACTCGGGTGC CTGACCCACG GATTCCAATG 
AACGCGGCCG TCAGGACTTC TAATCTGCCA CAGCGCAACC TCAGTTTCCG CGAGAGAGCA 
GCTAATCAGG AGGCCAAGCT GCCCAATGAT ATCAATGAAG CCTTGGGCCC CATGCCCGCA 
CCAAGGAAGA TGGTGAAGAA GCCGGTAGCA GAGCCATGGC CAAGGCAAAG CCAAGAAAAG 
CAGGAGGCCC TGGAGTCAGC AGAGGTTCGA GATCATCAAC CAGTTGAGGA TACAGTCGAA 
AGGGCTGTCA AGGATGACCA AAGTCAGCAG TTCGGCAATA TCACTATTGG AGCGAGCGCA 
GAGCCAGTCA GAGTAGCGAG CCAGAGAAAG GCGGACAAAA TGCTTGGTCG TGCCCCCGTC 
CAGCAACCCC GGCAGTATAC AACACCTACG GAGCGACAAA TCCAAGTTCA GACCGAGCCC 
GACCCCCAAC CTCCCGTCGA AAGTCCCATC ATTCCTGCCG AAACCCCACA GGCTACAACC 
GAAAGGATGC CCATGCGCGG GCCTAACCAT CCTCACCCAC CTAACTACCA GCACCGACAC 
CAGCACCAGG CACGAAGAGA TGTTAGACGT GACGACCAAG GGAGAGATCT TAGACGCGAC 
CGCGATGATG CTGAGCCTGG CCATCATATC TCTAATATTA TGTACCATGC GCGGGACGAC 
CTCGAACCCG GGCAGGGCAA GTTTCAACCA ACAAAATACC TAGAAGAGTG GAAGAATGGC 
ACAGTCGGTA CTCTTGGCGG CCCGCTTCTT GACCTGGGGA ATGTATCGCC TGTAGGAGAT 
AAGGGCGCAC CCCAGTTTCG GAGACGAGGT ACCGGGGGAC TGCGGCCAAG GAGAGGTGAA 
GCTTTTGATG GGGAGTATGA TGACACTTAT GCTCCCACGA GATTCAAGCC GCCACTCTAC 
CTCAAATGTG GCCCTCTTCT ACGGTTTTGC GGTATCCGGC ATGACCGTGT GGCCACCCGC 
TCCCCCAAAC CCGGGGCTGT GTTAGAGAAA GAGATATGGC GCGGTTCAGT CATGATCGTC 
ACATCAGACG CCGATTCATC ATACGATATT GCCCCAACAC TGAGGCTATT TGCTCAACCG 
GTGGAACTCT TACCCCCTCC GCCGAAAATT ATACATGGTG ATGAGCCTTT GGCAGCAGAG 
TATGTTGACC CCATCGCAGG CCATCCTAAA TTAGGACGCA AGGGCGAGAC TCTCTATGTC 
AGACCGGTCG ACCATCTGGA CGAAGGCAAG GATCTTTCCC GGGATGAGAC CGACAACGGA 
TTATTCGAAA AGTCGAGAAG CGCGCCAGAG TCTCTTCCGA CCGGCGTAAC TGACCCGCCC 
GGCTCGTTCA ACGCCCGAAA GAAACGCGCC AGGATTGATG GTGAGAAAGT AGGAAAGTAC 
AAAGACGTCC GAGGTTTCCG ACTTCACGCT GAGCGAGGAT ACACTTTCTG GCGATTTAAT 
ATCGAAGTCG AACTCCGCGA AAAACAACAA CGCATCGCTT ATCGGATCAA CAGGGGACCG 
GCGACGGGTT TTTGGGTCCC AGCCAAAGGA CAGGCAATGA ACATAATGTT CCACAGCTGC 
AACGGTTTTA GCTTGAGCGT CGATCCTAAC CAGTTCAGCG GCCCCGACCC TATGTGGCGC 
GACGTGCTCA ACACTCACCA GAGCCAACCG TTCCATGTGA TGATCGGTGG TGGTGACCAA 
ATCTACAACG ACAAGTGCAT GCAAGAGACA CAGCTCTTCA AGGAATGGCT GTTAATCAAG 
GACCCATCGC ACAAGCATAA CATTCCTTTC AGCGCGGAGA TGCAGGACGA GCTAGAGCGC 
TTCTACCTGG AGCGATACGC CATGTGGTTT TCGCAGGGTT TGTTTGGCAT GGCAGCCTCA 
CAAATCCCCA TGGTCAACAT GTATGATGAC CACGACATCT TTGACGGCTT TGGCTCGTAT 
CCCCATCATT TCATGGAGTC CCCCGTGTTC TCGGGACTTG GCAATGTTGC TTTCAAGTAC 
TATATGCTGT TCCAGCATCA GAGCGTGGTG GATGAAACAG AGAAATCCGA GCCAAGCTGG 
ATCTTAGGTG AAAAACCGGG CCCGTACATC CAGGAGGTCA GTCGAAGTAT GTTTATGTTC 
TTGGGCGCAA AGGTTGCACT ACTGGCGGTT GACGCACGGA CTGAGCGAAC GCGAGAGGAA 
GTCATTAGAG AAAATACATG GAAGAGAATT ATGGACCGGT GTTATGGTGA AATCGAAAAT 
GGGAAGGTAG AGCATCTGCT GGTCCTTCTT GGTGTGCCGG TCGCTTACCC GAGGCTGGTC 
TGGCTGGAAA ATATCTTGAC CTCTCGTCTG ATGGATCCTG TCAAGGCTCT TGGAAGAGCC 
GGCCTTTTCA AAAACCTCCT GAACCATTTC GATGGAGGCG TTGAAGTCCT GGATGATTTG 
GACGACCACT GGACAGCCAA AAACCATAAG GAGGAGCGGG CAATTGTGAT CGAAGATCTA 
CAGGACCTGG CAGCTGACAA GTCCGTCAGA GTTACTATCC TCAGCGGTGA CGTCCACTTG 
GCAGCCATCG GGCAATTTTA CTCGAACCCC AAGCTCAACA TCCCGAAGGA CAAGGACTTC 
AGGTACATGC CAAACGTCAT CTCCTCCGCC ATCGTCAACG CTCCGCCTTC AGATATGTTG 
GCCGACGTGC TCAACAAACG TAACAAGGTG CATCATTTCG ACCGAGACAC GGACGAAGAC 
ATGATCCCAA TGTTCCAGCA AGGTGTCGAT GGCAAGCCAC GCAACAACAA GCATCTGCTT 
CCACACCGCA ACTGGTGCTC AATTCGGCTG TATAACCCCG GGAACACACC AGACTCCACA 
CCCGGCCAGT CTGTAATTGG CATCAACGCT TACGGACTAC CTTCCAACGG ACCCAGCCTT 
TTACGTCGCC TCTCACTTTC CAAAACACGA AGCCAATCCG GCCCCACATA CCGTGGCCCC 
GACAGCGTGC GCGACCGAAC CCGCCCGCCC ATCTCCAACG CGTTGCTCCG CACTCTTTCC 
CGCCGCGGAC CAAGCGCAAG CGCTGATCAA GTCGTCCCTG GCGGCCGCGG AAGCCCAGCG 
GCAGCAGGAA AGCCCCCCGG GTTGTTGAAG AGGACCCTCT CGGGCTCCTC CATAAGCGGT 
AAAGTCGGCG ACTTCTTCCG CCGACGTGAC AGCAACAGCT CCCAGCGGCC GCGTGTGGGC 
GATGAGGGCA TTAACGGGAC TTGGGTTCCG GAATCGGACG CTGAACAGGC TGTTTACGAT 
GACTATGCCC ATGTTGGGGA TGAGTATAAC ATCCCTAAGA TCCGCGGTGG CATTCGCGAT 
GGCATGTCTG CTCGTGTGGG CTTGCGCGGT GGAGGGGGTG AATATTATCC TACTCCCCCC 
GTGCCCGCTG GTACTGGTGG TGACGAGTTT GTTGCCGGTG ACGAGAGTTA CTTTACGGCC 
AAACCGGTCT CTGATCACAA TCGTGGAGTT AGTACCGGTG GCGAGTATCC CTCTTCACCA 
ATGACCGGTT TCAGTAACAC CGGCCGGGGT GGTCCCACGG GCATGGACAT GGGCATATTC 
CAGTCCCAAG CAACATCCGC CTCCTTACCA GAACGGGACT TTGCACCTTC TCAACCCTCA 
CCTCGTAGTG GCGGCAGTAT GTTCCGTTCC AGAACAACAG GCGGTGACAC AAGAACCATA 
AAGCGCGGAG ACGCCATACC TCGAATCATG GCCGACGGCG CACTGGAAGT CACCCTCAAC 
GTGGAGATCA GCCCGCGCGA TCCGGGAGGC AGCACGGTGC CGTACCGCCT GCTGGTGCCC 
AAGTTGTCGT ATGAGTATGA GGGCGAGGAT CATGACCACG ATCACGATCC GGAGATGAAT 
GCGATGGTTG GGGAGGAGCG TACGTCGAGC CCGGGGCAGG TGCAGACGTT AGAGTCTAGG 
CAGCGGGAGG AGACGCGGAC GGAGATGGGG ATGGAGATGG GAATGGTGAT GGGGATGGAG 
GAGAAGCCTA TCACTAGTGG TGGTGGAGGG CTTAGACGGT TGCTTAGTTT AAAAAGGAAT 
GGGCGACGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03220 ( XM_011394814.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393116.1 Neurospora crassa OR74A uncharacterized protein (NCU03220), mRNA.

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CloneID	ONf13149
Clone ID Related Accession (Same CDS sequence)	XM_011394814.1
Accession Version	XM_011394814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGCGAGCAC GAGCTGGCGA CCACAGCGTA TCGTTAACGG ATCGACGCGT TCGGCAAACA 
TGCCACCATC TGCGGGAGCA AACAGCCACC GACGCAGGGT TTCTGGTCTT GACCAGCCTC 
TACAGAGGAG TACTAGGCGC TCGTCAGCGA GCCAGCCAGC AACCCGACAG AGAATCAAAA 
GAAGAATCAG GGGAAGGACC TACTCCACAA GCCGAAGCCA CCGACGGCCC AACACGAAAA 
GGTGCGCCAG AGAACTCGGC ATATGAACAG ACCGAAGTAA AGACGATATC GCCGCAAGTT 
CCTGTCCAGA TTCAACAGGT CGCGCTCGCT TTTCAACGTC AGGATCAACC CTCACAGACT 
ATGCCGGTAA GTTCGACGAC TTCAACCAGA CCTGACCAAG GTTCGGCGGC TGCAAAGGGA 
CCTCTCTCGC AGAATCCTCC CGAAGAAGGG CGAACGTATG GAGCAAACGG TGGGCGAGTT 
GTTAGAACTC GGGTGCCTGA CCCACGGATT CCAATGAACG CGGCCGTCAG GACTTCTAAT 
CTGCCACAGC GCAACCTCAG TTTCCGCGAG AGAGCAGCTA ATCAGGAGGC CAAGCTGCCC 
AATGATATCA ATGAAGCCTT GGGCCCCATG CCCGCACCAA GGAAGATGGT GAAGAAGCCG 
GTAGCAGAGC CATGGCCAAG GCAAAGCCAA GAAAAGCAGG AGGCCCTGGA GTCAGCAGAG 
GTTCGAGATC ATCAACCAGT TGAGGATACA GTCGAAAGGG CTGTCAAGGA TGACCAAAGT 
CAGCAGTTCG GCAATATCAC TATTGGAGCG AGCGCAGAGC CAGTCAGAGT AGCGAGCCAG 
AGAAAGGCGG ACAAAATGCT TGGTCGTGCC CCCGTCCAGC AACCCCGGCA GTATACAACA 
CCTACGGAGC GACAAATCCA AGTTCAGACC GAGCCCGACC CCCAACCTCC CGTCGAAAGT 
CCCATCATTC CTGCCGAAAC CCCACAGGCT ACAACCGAAA GGATGCCCAT GCGCGGGCCT 
AACCATCCTC ACCCACCTAA CTACCAGCAC CGACACCAGC ACCAGGCACG AAGAGATGTT 
AGACGTGACG ACCAAGGGAG AGATCTTAGA CGCGACCGCG ATGATGCTGA GCCTGGCCAT 
CATATCTCTA ATATTATGTA CCATGCGCGG GACGACCTCG AACCCGGGCA GGGCAAGTTT 
CAACCAACAA AATACCTAGA AGAGTGGAAG AATGGCACAG TCGGTACTCT TGGCGGCCCG 
CTTCTTGACC TGGGGAATGT ATCGCCTGTA GGAGATAAGG GCGCACCCCA GTTTCGGAGA 
CGAGGTACCG GGGGACTGCG GCCAAGGAGA GGTGAAGCTT TTGATGGGGA GTATGATGAC 
ACTTATGCTC CCACGAGATT CAAGCCGCCA CTCTACCTCA AATGTGGCCC TCTTCTACGG 
TTTTGCGGTA TCCGGCATGA CCGTGTGGCC ACCCGCTCCC CCAAACCCGG GGCTGTGTTA 
GAGAAAGAGA TATGGCGCGG TTCAGTCATG ATCGTCACAT CAGACGCCGA TTCATCATAC 
GATATTGCCC CAACACTGAG GCTATTTGCT CAACCGGTGG AACTCTTACC CCCTCCGCCG 
AAAATTATAC ATGGTGATGA GCCTTTGGCA GCAGAGTATG TTGACCCCAT CGCAGGCCAT 
CCTAAATTAG GACGCAAGGG CGAGACTCTC TATGTCAGAC CGGTCGACCA TCTGGACGAA 
GGCAAGGATC TTTCCCGGGA TGAGACCGAC AACGGATTAT TCGAAAAGTC GAGAAGCGCG 
CCAGAGTCTC TTCCGACCGG CGTAACTGAC CCGCCCGGCT CGTTCAACGC CCGAAAGAAA 
CGCGCCAGGA TTGATGGTGA GAAAGTAGGA AAGTACAAAG ACGTCCGAGG TTTCCGACTT 
CACGCTGAGC GAGGATACAC TTTCTGGCGA TTTAATATCG AAGTCGAACT CCGCGAAAAA 
CAACAACGCA TCGCTTATCG GATCAACAGG GGACCGGCGA CGGGTTTTTG GGTCCCAGCC 
AAAGGACAGG CAATGAACAT AATGTTCCAC AGCTGCAACG GTTTTAGCTT GAGCGTCGAT 
CCTAACCAGT TCAGCGGCCC CGACCCTATG TGGCGCGACG TGCTCAACAC TCACCAGAGC 
CAACCGTTCC ATGTGATGAT CGGTGGTGGT GACCAAATCT ACAACGACAA GTGCATGCAA 
GAGACACAGC TCTTCAAGGA ATGGCTGTTA ATCAAGGACC CATCGCACAA GCATAACATT 
CCTTTCAGCG CGGAGATGCA GGACGAGCTA GAGCGCTTCT ACCTGGAGCG ATACGCCATG 
TGGTTTTCGC AGGGTTTGTT TGGCATGGCA GCCTCACAAA TCCCCATGGT CAACATGTAT 
GATGACCACG ACATCTTTGA CGGCTTTGGC TCGTATCCCC ATCATTTCAT GGAGTCCCCC 
GTGTTCTCGG GACTTGGCAA TGTTGCTTTC AAGTACTATA TGCTGTTCCA GCATCAGAGC 
GTGGTGGATG AAACAGAGAA ATCCGAGCCA AGCTGGATCT TAGGTGAAAA ACCGGGCCCG 
TACATCCAGG AGGTCAGTCG AAGTATGTTT ATGTTCTTGG GCGCAAAGGT TGCACTACTG 
GCGGTTGACG CACGGACTGA GCGAACGCGA GAGGAAGTCA TTAGAGAAAA TACATGGAAG 
AGAATTATGG ACCGGTGTTA TGGTGAAATC GAAAATGGGA AGGTAGAGCA TCTGCTGGTC 
CTTCTTGGTG TGCCGGTCGC TTACCCGAGG CTGGTCTGGC TGGAAAATAT CTTGACCTCT 
CGTCTGATGG ATCCTGTCAA GGCTCTTGGA AGAGCCGGCC TTTTCAAAAA CCTCCTGAAC 
CATTTCGATG GAGGCGTTGA AGTCCTGGAT GATTTGGACG ACCACTGGAC AGCCAAAAAC 
CATAAGGAGG AGCGGGCAAT TGTGATCGAA GATCTACAGG ACCTGGCAGC TGACAAGTCC 
GTCAGAGTTA CTATCCTCAG CGGTGACGTC CACTTGGCAG CCATCGGGCA ATTTTACTCG 
AACCCCAAGC TCAACATCCC GAAGGACAAG GACTTCAGGT ACATGCCAAA CGTCATCTCC 
TCCGCCATCG TCAACGCTCC GCCTTCAGAT ATGTTGGCCG ACGTGCTCAA CAAACGTAAC 
AAGGTGCATC ATTTCGACCG AGACACGGAC GAAGACATGA TCCCAATGTT CCAGCAAGGT 
GTCGATGGCA AGCCACGCAA CAACAAGCAT CTGCTTCCAC ACCGCAACTG GTGCTCAATT 
CGGCTGTATA ACCCCGGGAA CACACCAGAC TCCACACCCG GCCAGTCTGT AATTGGCATC 
AACGCTTACG GACTACCTTC CAACGGACCC AGCCTTTTAC GTCGCCTCTC ACTTTCCAAA 
ACACGAAGCC AATCCGGCCC CACATACCGT GGCCCCGACA GCGTGCGCGA CCGAACCCGC 
CCGCCCATCT CCAACGCGTT GCTCCGCACT CTTTCCCGCC GCGGACCAAG CGCAAGCGCT 
GATCAAGTCG TCCCTGGCGG CCGCGGAAGC CCAGCGGCAG CAGGAAAGCC CCCCGGGTTG 
TTGAAGAGGA CCCTCTCGGG CTCCTCCATA AGCGGTAAAG TCGGCGACTT CTTCCGCCGA 
CGTGACAGCA ACAGCTCCCA GCGGCCGCGT GTGGGCGATG AGGGCATTAA CGGGACTTGG 
GTTCCGGAAT CGGACGCTGA ACAGGCTGTT TACGATGACT ATGCCCATGT TGGGGATGAG 
TATAACATCC CTAAGATCCG CGGTGGCATT CGCGATGGCA TGTCTGCTCG TGTGGGCTTG 
CGCGGTGGAG GGGGTGAATA TTATCCTACT CCCCCCGTGC CCGCTGGTAC TGGTGGTGAC 
GAGTTTGTTG CCGGTGACGA GAGTTACTTT ACGGCCAAAC CGGTCTCTGA TCACAATCGT 
GGAGTTAGTA CCGGTGGCGA GTATCCCTCT TCACCAATGA CCGGTTTCAG TAACACCGGC 
CGGGGTGGTC CCACGGGCAT GGACATGGGC ATATTCCAGT CCCAAGCAAC ATCCGCCTCC 
TTACCAGAAC GGGACTTTGC ACCTTCTCAA CCCTCACCTC GTAGTGGCGG CAGTATGTTC 
CGTTCCAGAA CAACAGGCGG TGACACAAGA ACCATAAAGC GCGGAGACGC CATACCTCGA 
ATCATGGCCG ACGGCGCACT GGAAGTCACC CTCAACGTGG AGATCAGCCC GCGCGATCCG 
GGAGGCAGCA CGGTGCCGTA CCGCCTGCTG GTGCCCAAGT TGTCGTATGA GTATGAGGGC 
GAGGATCATG ACCACGATCA CGATCCGGAG ATGAATGCGA TGGTTGGGGA GGAGCGTACG 
TCGAGCCCGG GGCAGGTGCA GACGTTAGAG TCTAGGCAGC GGGAGGAGAC GCGGACGGAG 
ATGGGGATGG AGATGGGAAT GGTGATGGGG ATGGAGGAGA AGCCTATCAC TAGTGGTGGT 
GGAGGGCTTA GACGGTTGCT TAGTTTAAAA AGGAATGGGC GACGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393116.1
CDS	1455..6002
Translation	MRARAGDHSVSLTDRRVRQTCHHLREQTATDAGFLVLTSLYRGVLGARQRASQQPDRESKEESGEGPTPQAEATDGPTRKGAPENSAYEQTEVKTISPQVPVQIQQVALAFQRQDQPSQTMPVSSTTSTRPDQGSAAAKGPLSQNPPEEGRTYGANGGRVVRTRVPDPRIPMNAAVRTSNLPQRNLSFRERAANQEAKLPNDINEALGPMPAPRKMVKKPVAEPWPRQSQEKQEALESAEVRDHQPVEDTVERAVKDDQSQQFGNITIGASAEPVRVASQRKADKMLGRAPVQQPRQYTTPTERQIQVQTEPDPQPPVESPIIPAETPQATTERMPMRGPNHPHPPNYQHRHQHQARRDVRRDDQGRDLRRDRDDAEPGHHISNIMYHARDDLEPGQGKFQPTKYLEEWKNGTVGTLGGPLLDLGNVSPVGDKGAPQFRRRGTGGLRPRRGEAFDGEYDDTYAPTRFKPPLYLKCGPLLRFCGIRHDRVATRSPKPGAVLEKEIWRGSVMIVTSDADSSYDIAPTLRLFAQPVELLPPPPKIIHGDEPLAAEYVDPIAGHPKLGRKGETLYVRPVDHLDEGKDLSRDETDNGLFEKSRSAPESLPTGVTDPPGSFNARKKRARIDGEKVGKYKDVRGFRLHAERGYTFWRFNIEVELREKQQRIAYRINRGPATGFWVPAKGQAMNIMFHSCNGFSLSVDPNQFSGPDPMWRDVLNTHQSQPFHVMIGGGDQIYNDKCMQETQLFKEWLLIKDPSHKHNIPFSAEMQDELERFYLERYAMWFSQGLFGMAASQIPMVNMYDDHDIFDGFGSYPHHFMESPVFSGLGNVAFKYYMLFQHQSVVDETEKSEPSWILGEKPGPYIQEVSRSMFMFLGAKVALLAVDARTERTREEVIRENTWKRIMDRCYGEIENGKVEHLLVLLGVPVAYPRLVWLENILTSRLMDPVKALGRAGLFKNLLNHFDGGVEVLDDLDDHWTAKNHKEERAIVIEDLQDLAADKSVRVTILSGDVHLAAIGQFYSNPKLNIPKDKDFRYMPNVISSAIVNAPPSDMLADVLNKRNKVHHFDRDTDEDMIPMFQQGVDGKPRNNKHLLPHRNWCSIRLYNPGNTPDSTPGQSVIGINAYGLPSNGPSLLRRLSLSKTRSQSGPTYRGPDSVRDRTRPPISNALLRTLSRRGPSASADQVVPGGRGSPAAAGKPPGLLKRTLSGSSISGKVGDFFRRRDSNSSQRPRVGDEGINGTWVPESDAEQAVYDDYAHVGDEYNIPKIRGGIRDGMSARVGLRGGGGEYYPTPPVPAGTGGDEFVAGDESYFTAKPVSDHNRGVSTGGEYPSSPMTGFSNTGRGGPTGMDMGIFQSQATSASLPERDFAPSQPSPRSGGSMFRSRTTGGDTRTIKRGDAIPRIMADGALEVTLNVEISPRDPGGSTVPYRLLVPKLSYEYEGEDHDHDHDPEMNAMVGEERTSSPGQVQTLESRQREETRTEMGMEMGMVMGMEEKPITSGGGGLRRLLSLKRNGRR

Target ORF information:

RefSeq Version	XM_011394814.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A uncharacterized protein (NCU03220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394814.1

ORF Insert Sequence:

ATGCGAGCAC GAGCTGGCGA CCACAGCGTA TCGTTAACGG ATCGACGCGT TCGGCAAACA 
TGCCACCATC TGCGGGAGCA AACAGCCACC GACGCAGGGT TTCTGGTCTT GACCAGCCTC 
TACAGAGGAG TACTAGGCGC TCGTCAGCGA GCCAGCCAGC AACCCGACAG AGAATCAAAA 
GAAGAATCAG GGGAAGGACC TACTCCACAA GCCGAAGCCA CCGACGGCCC AACACGAAAA 
GGTGCGCCAG AGAACTCGGC ATATGAACAG ACCGAAGTAA AGACGATATC GCCGCAAGTT 
CCTGTCCAGA TTCAACAGGT CGCGCTCGCT TTTCAACGTC AGGATCAACC CTCACAGACT 
ATGCCGGTAA GTTCGACGAC TTCAACCAGA CCTGACCAAG GTTCGGCGGC TGCAAAGGGA 
CCTCTCTCGC AGAATCCTCC CGAAGAAGGG CGAACGTATG GAGCAAACGG TGGGCGAGTT 
GTTAGAACTC GGGTGCCTGA CCCACGGATT CCAATGAACG CGGCCGTCAG GACTTCTAAT 
CTGCCACAGC GCAACCTCAG TTTCCGCGAG AGAGCAGCTA ATCAGGAGGC CAAGCTGCCC 
AATGATATCA ATGAAGCCTT GGGCCCCATG CCCGCACCAA GGAAGATGGT GAAGAAGCCG 
GTAGCAGAGC CATGGCCAAG GCAAAGCCAA GAAAAGCAGG AGGCCCTGGA GTCAGCAGAG 
GTTCGAGATC ATCAACCAGT TGAGGATACA GTCGAAAGGG CTGTCAAGGA TGACCAAAGT 
CAGCAGTTCG GCAATATCAC TATTGGAGCG AGCGCAGAGC CAGTCAGAGT AGCGAGCCAG 
AGAAAGGCGG ACAAAATGCT TGGTCGTGCC CCCGTCCAGC AACCCCGGCA GTATACAACA 
CCTACGGAGC GACAAATCCA AGTTCAGACC GAGCCCGACC CCCAACCTCC CGTCGAAAGT 
CCCATCATTC CTGCCGAAAC CCCACAGGCT ACAACCGAAA GGATGCCCAT GCGCGGGCCT 
AACCATCCTC ACCCACCTAA CTACCAGCAC CGACACCAGC ACCAGGCACG AAGAGATGTT 
AGACGTGACG ACCAAGGGAG AGATCTTAGA CGCGACCGCG ATGATGCTGA GCCTGGCCAT 
CATATCTCTA ATATTATGTA CCATGCGCGG GACGACCTCG AACCCGGGCA GGGCAAGTTT 
CAACCAACAA AATACCTAGA AGAGTGGAAG AATGGCACAG TCGGTACTCT TGGCGGCCCG 
CTTCTTGACC TGGGGAATGT ATCGCCTGTA GGAGATAAGG GCGCACCCCA GTTTCGGAGA 
CGAGGTACCG GGGGACTGCG GCCAAGGAGA GGTGAAGCTT TTGATGGGGA GTATGATGAC 
ACTTATGCTC CCACGAGATT CAAGCCGCCA CTCTACCTCA AATGTGGCCC TCTTCTACGG 
TTTTGCGGTA TCCGGCATGA CCGTGTGGCC ACCCGCTCCC CCAAACCCGG GGCTGTGTTA 
GAGAAAGAGA TATGGCGCGG TTCAGTCATG ATCGTCACAT CAGACGCCGA TTCATCATAC 
GATATTGCCC CAACACTGAG GCTATTTGCT CAACCGGTGG AACTCTTACC CCCTCCGCCG 
AAAATTATAC ATGGTGATGA GCCTTTGGCA GCAGAGTATG TTGACCCCAT CGCAGGCCAT 
CCTAAATTAG GACGCAAGGG CGAGACTCTC TATGTCAGAC CGGTCGACCA TCTGGACGAA 
GGCAAGGATC TTTCCCGGGA TGAGACCGAC AACGGATTAT TCGAAAAGTC GAGAAGCGCG 
CCAGAGTCTC TTCCGACCGG CGTAACTGAC CCGCCCGGCT CGTTCAACGC CCGAAAGAAA 
CGCGCCAGGA TTGATGGTGA GAAAGTAGGA AAGTACAAAG ACGTCCGAGG TTTCCGACTT 
CACGCTGAGC GAGGATACAC TTTCTGGCGA TTTAATATCG AAGTCGAACT CCGCGAAAAA 
CAACAACGCA TCGCTTATCG GATCAACAGG GGACCGGCGA CGGGTTTTTG GGTCCCAGCC 
AAAGGACAGG CAATGAACAT AATGTTCCAC AGCTGCAACG GTTTTAGCTT GAGCGTCGAT 
CCTAACCAGT TCAGCGGCCC CGACCCTATG TGGCGCGACG TGCTCAACAC TCACCAGAGC 
CAACCGTTCC ATGTGATGAT CGGTGGTGGT GACCAAATCT ACAACGACAA GTGCATGCAA 
GAGACACAGC TCTTCAAGGA ATGGCTGTTA ATCAAGGACC CATCGCACAA GCATAACATT 
CCTTTCAGCG CGGAGATGCA GGACGAGCTA GAGCGCTTCT ACCTGGAGCG ATACGCCATG 
TGGTTTTCGC AGGGTTTGTT TGGCATGGCA GCCTCACAAA TCCCCATGGT CAACATGTAT 
GATGACCACG ACATCTTTGA CGGCTTTGGC TCGTATCCCC ATCATTTCAT GGAGTCCCCC 
GTGTTCTCGG GACTTGGCAA TGTTGCTTTC AAGTACTATA TGCTGTTCCA GCATCAGAGC 
GTGGTGGATG AAACAGAGAA ATCCGAGCCA AGCTGGATCT TAGGTGAAAA ACCGGGCCCG 
TACATCCAGG AGGTCAGTCG AAGTATGTTT ATGTTCTTGG GCGCAAAGGT TGCACTACTG 
GCGGTTGACG CACGGACTGA GCGAACGCGA GAGGAAGTCA TTAGAGAAAA TACATGGAAG 
AGAATTATGG ACCGGTGTTA TGGTGAAATC GAAAATGGGA AGGTAGAGCA TCTGCTGGTC 
CTTCTTGGTG TGCCGGTCGC TTACCCGAGG CTGGTCTGGC TGGAAAATAT CTTGACCTCT 
CGTCTGATGG ATCCTGTCAA GGCTCTTGGA AGAGCCGGCC TTTTCAAAAA CCTCCTGAAC 
CATTTCGATG GAGGCGTTGA AGTCCTGGAT GATTTGGACG ACCACTGGAC AGCCAAAAAC 
CATAAGGAGG AGCGGGCAAT TGTGATCGAA GATCTACAGG ACCTGGCAGC TGACAAGTCC 
GTCAGAGTTA CTATCCTCAG CGGTGACGTC CACTTGGCAG CCATCGGGCA ATTTTACTCG 
AACCCCAAGC TCAACATCCC GAAGGACAAG GACTTCAGGT ACATGCCAAA CGTCATCTCC 
TCCGCCATCG TCAACGCTCC GCCTTCAGAT ATGTTGGCCG ACGTGCTCAA CAAACGTAAC 
AAGGTGCATC ATTTCGACCG AGACACGGAC GAAGACATGA TCCCAATGTT CCAGCAAGGT 
GTCGATGGCA AGCCACGCAA CAACAAGCAT CTGCTTCCAC ACCGCAACTG GTGCTCAATT 
CGGCTGTATA ACCCCGGGAA CACACCAGAC TCCACACCCG GCCAGTCTGT AATTGGCATC 
AACGCTTACG GACTACCTTC CAACGGACCC AGCCTTTTAC GTCGCCTCTC ACTTTCCAAA 
ACACGAAGCC AATCCGGCCC CACATACCGT GGCCCCGACA GCGTGCGCGA CCGAACCCGC 
CCGCCCATCT CCAACGCGTT GCTCCGCACT CTTTCCCGCC GCGGACCAAG CGCAAGCGCT 
GATCAAGTCG TCCCTGGCGG CCGCGGAAGC CCAGCGGCAG CAGGAAAGCC CCCCGGGTTG 
TTGAAGAGGA CCCTCTCGGG CTCCTCCATA AGCGGTAAAG TCGGCGACTT CTTCCGCCGA 
CGTGACAGCA ACAGCTCCCA GCGGCCGCGT GTGGGCGATG AGGGCATTAA CGGGACTTGG 
GTTCCGGAAT CGGACGCTGA ACAGGCTGTT TACGATGACT ATGCCCATGT TGGGGATGAG 
TATAACATCC CTAAGATCCG CGGTGGCATT CGCGATGGCA TGTCTGCTCG TGTGGGCTTG 
CGCGGTGGAG GGGGTGAATA TTATCCTACT CCCCCCGTGC CCGCTGGTAC TGGTGGTGAC 
GAGTTTGTTG CCGGTGACGA GAGTTACTTT ACGGCCAAAC CGGTCTCTGA TCACAATCGT 
GGAGTTAGTA CCGGTGGCGA GTATCCCTCT TCACCAATGA CCGGTTTCAG TAACACCGGC 
CGGGGTGGTC CCACGGGCAT GGACATGGGC ATATTCCAGT CCCAAGCAAC ATCCGCCTCC 
TTACCAGAAC GGGACTTTGC ACCTTCTCAA CCCTCACCTC GTAGTGGCGG CAGTATGTTC 
CGTTCCAGAA CAACAGGCGG TGACACAAGA ACCATAAAGC GCGGAGACGC CATACCTCGA 
ATCATGGCCG ACGGCGCACT GGAAGTCACC CTCAACGTGG AGATCAGCCC GCGCGATCCG 
GGAGGCAGCA CGGTGCCGTA CCGCCTGCTG GTGCCCAAGT TGTCGTATGA GTATGAGGGC 
GAGGATCATG ACCACGATCA CGATCCGGAG ATGAATGCGA TGGTTGGGGA GGAGCGTACG 
TCGAGCCCGG GGCAGGTGCA GACGTTAGAG TCTAGGCAGC GGGAGGAGAC GCGGACGGAG 
ATGGGGATGG AGATGGGAAT GGTGATGGGG ATGGAGGAGA AGCCTATCAC TAGTGGTGGT 
GGAGGGCTTA GACGGTTGCT TAGTTTAAAA AGGAATGGGC GACGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03220 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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