nca-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	nca-1
Entrez Gene ID	3880631
Full Name	calcium P-type ATPase-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959389.3	XP_964482.3	calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP2A1	NP_775293.1
Pan troglodytes (chimpanzee)	ATP2A1	XP_001142958.3
Magnaporthe oryzae (rice blast fungus)	MGG_04550	XP_003710933.1
Arabidopsis thaliana (thale cress)	ECA3	NP_563860.1
Xenopus tropicalis (tropical clawed frog)	atp2a1	NP_001027498.1
Rattus norvegicus (Norway rat)	Atp2a1	NP_478120.1
Neurospora crassa	NCU03305	XP_964482.2
Mus musculus (house mouse)	Atp2a1	NP_031530.2
Danio rerio (zebrafish)	atp2a1	NP_001007030.1
Caenorhabditis elegans (roundworm)	sca-1	NP_499386.1
Oryza sativa (rice)	Os03g0730800	NP_001051163.2
Danio rerio (zebrafish)	atp2a1l	NP_001071001.1
Anopheles gambiae (African malaria mosquito)	ATC1_ANOGA	XP_001688815.1
Canis lupus familiaris (dog)	ATP2A1	XP_005621437.1
Bos taurus (cattle)	ATP2A1	NP_001069235.1
Drosophila melanogaster (fruit fly)	Ca-P60A	NP_726387.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nca-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nca-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12840	XM_959389.3 Latest version!	Neurospora crassa OR74A calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type (nca-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nca-1 ( XM_959389.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964482.3 Neurospora crassa OR74A calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type (nca-1), mRNA.

Price & Availability↑

CloneID	ONf12840
Clone ID Related Accession (Same CDS sequence)	XM_959389.3
Accession Version	XM_959389.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959389.2.

ATGGAGGCGG CGTTTGCAAA GCCCGTCGAT GAGGTCCTCA GCACCTTTGG TGTCGACCCT 
ACCACCGGTC TAAGTGACGA GCAAGTAGCT CAGTCCAGGG CTAAGTACGG TAGAAATGCC 
ATTCCAGAGG AACCTCCGAC ACCCATCTGG GAGCTTATCT TGGAACAATT CAAAGACCAG 
CTGGTCCTCA TCCTTCTGGG CTCTGCCGCC ATCTCGTTCG TACTCGCGCT CTTCGAAGAC 
GAGGGCGGCT GGAGCGCCTT CGTCGACCCC GCCGTCATCA TAACGATCCT AGTTCTCAAC 
GCTGTTGTCG GAGTGTCCCA GGAAAGCAGT GCCGAGAAGG CGATTGCTGC TTTGCAAGAG 
TATTCGGCCA ACGAGGCCAA CGTGGTGCGC AATGGGCAGA TTACTCGTAT CAAGGCCGAG 
GATCTTGTGC CAGGCGATGT CGTTGACATT GCTGTTGGTG CTCGCGTCCC TGCTGACTGC 
AGATTGATCT CGATTGAGAG TAACAGCTTT GCTGTGGACC AGGCCATCTT GACCGGAGAA 
AGCGAGAGCG TCGGGAAAGA CTTCCAGGCC GTTGTTAGCG ACGACAAGGC TGTCTTGCAG 
GACCAGGTCA ATATGCTGTT TTCGGGCACA ACTGTTGTCA CTGGTCACGC CAAAGCCGTC 
GTTGTCTTGA CTGGCTCCAA TACAGCTATC GGCGACATTC ACGAGAGCAT CACCGCCCAG 
ATCTCCGAGC CTACCCCCTT GAAGCAAAAG CTCAATGACT TCGGAGACCA GCTCGCCAAG 
GTTATCACTG TCATCTGCGT TCTCGTCTGG CTCATCAACA TCCCTAATTT CGCCGACCCT 
AGCCATGGAA ACTGGACCAA GGGTGCCATT TACTACCTCA AGATCGCCGT TTCCCTTGGC 
GTTGCGGCCA TCCCCGAGGG TCTTGCGGTC GTCATCACCA CCTGCCTTGC CCTTGGAACT 
CGCAAGATGG CAGCTAAGAA CGCCGTGGTG AGAAGTCTTC CGTCTGTTGA GACCCTGGGC 
AGTTGCAGCG TCATTTGCTC TGACAAGACC GGCACTTTGA CCACCAACCA AATGAGCGTT 
AACAAAATCG TCCATTTCAA CCAAGATGGT ACTGATCTGG AGGAGCTGGA TGTTGAGGGA 
ACTACCTTTG AACCCAAGGG TGCTATCATG TCGCAGGGCA AGAAGGTTAC CGACCTTGCT 
CAAAACTCCG CTACCATTCT TCAGTTGACC GAAGTTGCCG CTCTCTGCAA CGATGCCCGC 
CTTGACTACC ATCCCTCTAC CGGCACTTTC TCGAACGTGG GCGAAGCTAC CGAAGGCGCC 
CTCCGCGTTT TGACCGAGAA GATCGGCCCC TGCGCCCCCT CTGATTGTCC TCCCAAGGAT 
CGCGTTCACT ACGCTAGCTC CTGGTACGAG AAGCAGTATC AGCGTCTTGC CACCTACGAG 
TTTTCGCGTG ACCGCAAGAG CATGTCTGTC TTGGTTGAGC GTGATGGACA GCAGAAGCTT 
CTCGTTAAGG GAGCCCCTGA ATCGCTCATC GAACGCTGTA CTCACGCTCT CCTCGGTCCC 
GATGGCAAGA AGGTACCTTT GGACAGTAAC ATGTCCGAAC TCCTCATGAA GGAGGTTGTT 
GAGTATGGCA ACCGCGGTCT GCGTGTGATC GCACTGGCTA GTCTGGACAA CGTTGCTGGT 
AACCCTCTTC TTCACACCGC CAAGTCTACC GCCGAATATG CCTCTCTCGA ACAGAACCTT 
ACTCTCATCG GTCTCGTCGG CATGCTTGAC CCTCCGCGTC CTGAAGTTGC TGCTTCTATC 
AAGAAGTGCA AGGATGCAGG TATTCGCGTT GTCGTGATTA CCGGTGATAA CCGCAACACC 
GCTGAGAGCA TCTGCCGTCA GATCGGTGTC TTTGGATCTA ACGAGGATTT AACTGGCAAG 
AGCTACACTG GTCGCGAATT CGATAACCTT ACCCCTAGTG AACAGCTCGA GGCCGCCAAG 
ACTGCTTCTC TGTTCTCTCG CGTGGAGCCT ACTCACAAGT CTAAGCTGGT TGATCTCCTC 
CAGTCTCTCG GTGAGGTCGT TGCTATGACT GGCGACGGTG TCAACGATGC TCCTGCTCTG 
AAGAAGGCTG ATATTGGTGT TGCTATGGGT TCCGGTACCG ACGTGTCGAA GCTCGCTGCT 
GACATGGTGC TTGCTGATGA TAACTTTGCC ACTATCGAAG TCGCCATCGA GGAAGGCCGC 
GCCATCTACA ACAACACCCA GCAGTTCATC CGCTACCTCA TCTCTTCGAA CATCGGTGAG 
GTCGTCTCTA TCTTTTTGAC GGCCGCTCTC GGTATGCCTG AGGCTCTCAT CCCGGTTCAG 
CTTCTATGGG TGAACTTGGT CACCGACGGT CTCCCCGCTA CCGCCTTGTC CTTCAACCCC 
CCTGACCACG ATATCATGCG TCGCAACCCC AGAAAGCGTG ACGAGGCTCT CATCGGCGGA 
TGGCTCTTCT TCCGTTATCT GGTCATTGGT ACCTATGTCG GTCTCGCCAC GGTCGCCGGT 
TACGCTTGGT GGTTCATGTT CTACTCGGAG GGTCCTCAGA TCAGCTTCTA CCAGCTATCT 
CACTTCCATC GCTGCTCTAC CGAGTTCCCT GAGATTGGCT GCGCCATGTT CTCGAACGAC 
ATGGCCAAGG CCGGTTCGAC CGTCTCGCTC TCCATCTTGG TCGTCATTGA GATGTTCAAT 
GCCATGAACG CCCTCTCTTC CAGCGAGTCT CTCTTGACGC TCCCTGTTTG GAAGAACATG 
ATGCTCGTCT ATGCTATCGG TCTGTCCATG GCCTTGCACT TCGCCCTTCT CTACACTCCC 
ATCCTTCAGA CCCTCTTTTC GATTCTCCCC ATGAACTGGG CAGAGTGGAA AGCCGTGATC 
GTCATCAGTG CACCAGTTGT GCTTCTTGAC GAACTTCTCA AGGCCGTGGA ACGCAAGTAC 
TTTGTACAGA GCACCCCCAG CTCGACGTCC TCAGATAAGA AGAAGGATCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964482.3
CDS	345..3338
Translation	MEAAFAKPVDEVLSTFGVDPTTGLSDEQVAQSRAKYGRNAIPEEPPTPIWELILEQFKDQLVLILLGSAAISFVLALFEDEGGWSAFVDPAVIITILVLNAVVGVSQESSAEKAIAALQEYSANEANVVRNGQITRIKAEDLVPGDVVDIAVGARVPADCRLISIESNSFAVDQAILTGESESVGKDFQAVVSDDKAVLQDQVNMLFSGTTVVTGHAKAVVVLTGSNTAIGDIHESITAQISEPTPLKQKLNDFGDQLAKVITVICVLVWLINIPNFADPSHGNWTKGAIYYLKIAVSLGVAAIPEGLAVVITTCLALGTRKMAAKNAVVRSLPSVETLGSCSVICSDKTGTLTTNQMSVNKIVHFNQDGTDLEELDVEGTTFEPKGAIMSQGKKVTDLAQNSATILQLTEVAALCNDARLDYHPSTGTFSNVGEATEGALRVLTEKIGPCAPSDCPPKDRVHYASSWYEKQYQRLATYEFSRDRKSMSVLVERDGQQKLLVKGAPESLIERCTHALLGPDGKKVPLDSNMSELLMKEVVEYGNRGLRVIALASLDNVAGNPLLHTAKSTAEYASLEQNLTLIGLVGMLDPPRPEVAASIKKCKDAGIRVVVITGDNRNTAESICRQIGVFGSNEDLTGKSYTGREFDNLTPSEQLEAAKTASLFSRVEPTHKSKLVDLLQSLGEVVAMTGDGVNDAPALKKADIGVAMGSGTDVSKLAADMVLADDNFATIEVAIEEGRAIYNNTQQFIRYLISSNIGEVVSIFLTAALGMPEALIPVQLLWVNLVTDGLPATALSFNPPDHDIMRRNPRKRDEALIGGWLFFRYLVIGTYVGLATVAGYAWWFMFYSEGPQISFYQLSHFHRCSTEFPEIGCAMFSNDMAKAGSTVSLSILVVIEMFNAMNALSSSESLLTLPVWKNMMLVYAIGLSMALHFALLYTPILQTLFSILPMNWAEWKAVIVISAPVVLLDELLKAVERKYFVQSTPSSTSSDKKKDL

Target ORF information:

RefSeq Version	XM_959389.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type (nca-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959389.3

ORF Insert Sequence:

ATGGAGGCGG CGTTTGCAAA GCCCGTCGAT GAGGTCCTCA GCACCTTTGG TGTCGACCCT 
ACCACCGGTC TAAGTGACGA GCAAGTAGCT CAGTCCAGGG CTAAGTACGG TAGAAATGCC 
ATTCCAGAGG AACCTCCGAC ACCCATCTGG GAGCTTATCT TGGAACAATT CAAAGACCAG 
CTGGTCCTCA TCCTTCTGGG CTCTGCCGCC ATCTCGTTCG TACTCGCGCT CTTCGAAGAC 
GAGGGCGGCT GGAGCGCCTT CGTCGACCCC GCCGTCATCA TAACGATCCT AGTTCTCAAC 
GCTGTTGTCG GAGTGTCCCA GGAAAGCAGT GCCGAGAAGG CGATTGCTGC TTTGCAAGAG 
TATTCGGCCA ACGAGGCCAA CGTGGTGCGC AATGGGCAGA TTACTCGTAT CAAGGCCGAG 
GATCTTGTGC CAGGCGATGT CGTTGACATT GCTGTTGGTG CTCGCGTCCC TGCTGACTGC 
AGATTGATCT CGATTGAGAG TAACAGCTTT GCTGTGGACC AGGCCATCTT GACCGGAGAA 
AGCGAGAGCG TCGGGAAAGA CTTCCAGGCC GTTGTTAGCG ACGACAAGGC TGTCTTGCAG 
GACCAGGTCA ATATGCTGTT TTCGGGCACA ACTGTTGTCA CTGGTCACGC CAAAGCCGTC 
GTTGTCTTGA CTGGCTCCAA TACAGCTATC GGCGACATTC ACGAGAGCAT CACCGCCCAG 
ATCTCCGAGC CTACCCCCTT GAAGCAAAAG CTCAATGACT TCGGAGACCA GCTCGCCAAG 
GTTATCACTG TCATCTGCGT TCTCGTCTGG CTCATCAACA TCCCTAATTT CGCCGACCCT 
AGCCATGGAA ACTGGACCAA GGGTGCCATT TACTACCTCA AGATCGCCGT TTCCCTTGGC 
GTTGCGGCCA TCCCCGAGGG TCTTGCGGTC GTCATCACCA CCTGCCTTGC CCTTGGAACT 
CGCAAGATGG CAGCTAAGAA CGCCGTGGTG AGAAGTCTTC CGTCTGTTGA GACCCTGGGC 
AGTTGCAGCG TCATTTGCTC TGACAAGACC GGCACTTTGA CCACCAACCA AATGAGCGTT 
AACAAAATCG TCCATTTCAA CCAAGATGGT ACTGATCTGG AGGAGCTGGA TGTTGAGGGA 
ACTACCTTTG AACCCAAGGG TGCTATCATG TCGCAGGGCA AGAAGGTTAC CGACCTTGCT 
CAAAACTCCG CTACCATTCT TCAGTTGACC GAAGTTGCCG CTCTCTGCAA CGATGCCCGC 
CTTGACTACC ATCCCTCTAC CGGCACTTTC TCGAACGTGG GCGAAGCTAC CGAAGGCGCC 
CTCCGCGTTT TGACCGAGAA GATCGGCCCC TGCGCCCCCT CTGATTGTCC TCCCAAGGAT 
CGCGTTCACT ACGCTAGCTC CTGGTACGAG AAGCAGTATC AGCGTCTTGC CACCTACGAG 
TTTTCGCGTG ACCGCAAGAG CATGTCTGTC TTGGTTGAGC GTGATGGACA GCAGAAGCTT 
CTCGTTAAGG GAGCCCCTGA ATCGCTCATC GAACGCTGTA CTCACGCTCT CCTCGGTCCC 
GATGGCAAGA AGGTACCTTT GGACAGTAAC ATGTCCGAAC TCCTCATGAA GGAGGTTGTT 
GAGTATGGCA ACCGCGGTCT GCGTGTGATC GCACTGGCTA GTCTGGACAA CGTTGCTGGT 
AACCCTCTTC TTCACACCGC CAAGTCTACC GCCGAATATG CCTCTCTCGA ACAGAACCTT 
ACTCTCATCG GTCTCGTCGG CATGCTTGAC CCTCCGCGTC CTGAAGTTGC TGCTTCTATC 
AAGAAGTGCA AGGATGCAGG TATTCGCGTT GTCGTGATTA CCGGTGATAA CCGCAACACC 
GCTGAGAGCA TCTGCCGTCA GATCGGTGTC TTTGGATCTA ACGAGGATTT AACTGGCAAG 
AGCTACACTG GTCGCGAATT CGATAACCTT ACCCCTAGTG AACAGCTCGA GGCCGCCAAG 
ACTGCTTCTC TGTTCTCTCG CGTGGAGCCT ACTCACAAGT CTAAGCTGGT TGATCTCCTC 
CAGTCTCTCG GTGAGGTCGT TGCTATGACT GGCGACGGTG TCAACGATGC TCCTGCTCTG 
AAGAAGGCTG ATATTGGTGT TGCTATGGGT TCCGGTACCG ACGTGTCGAA GCTCGCTGCT 
GACATGGTGC TTGCTGATGA TAACTTTGCC ACTATCGAAG TCGCCATCGA GGAAGGCCGC 
GCCATCTACA ACAACACCCA GCAGTTCATC CGCTACCTCA TCTCTTCGAA CATCGGTGAG 
GTCGTCTCTA TCTTTTTGAC GGCCGCTCTC GGTATGCCTG AGGCTCTCAT CCCGGTTCAG 
CTTCTATGGG TGAACTTGGT CACCGACGGT CTCCCCGCTA CCGCCTTGTC CTTCAACCCC 
CCTGACCACG ATATCATGCG TCGCAACCCC AGAAAGCGTG ACGAGGCTCT CATCGGCGGA 
TGGCTCTTCT TCCGTTATCT GGTCATTGGT ACCTATGTCG GTCTCGCCAC GGTCGCCGGT 
TACGCTTGGT GGTTCATGTT CTACTCGGAG GGTCCTCAGA TCAGCTTCTA CCAGCTATCT 
CACTTCCATC GCTGCTCTAC CGAGTTCCCT GAGATTGGCT GCGCCATGTT CTCGAACGAC 
ATGGCCAAGG CCGGTTCGAC CGTCTCGCTC TCCATCTTGG TCGTCATTGA GATGTTCAAT 
GCCATGAACG CCCTCTCTTC CAGCGAGTCT CTCTTGACGC TCCCTGTTTG GAAGAACATG 
ATGCTCGTCT ATGCTATCGG TCTGTCCATG GCCTTGCACT TCGCCCTTCT CTACACTCCC 
ATCCTTCAGA CCCTCTTTTC GATTCTCCCC ATGAACTGGG CAGAGTGGAA AGCCGTGATC 
GTCATCAGTG CACCAGTTGT GCTTCTTGAC GAACTTCTCA AGGCCGTGGA ACGCAAGTAC 
TTTGTACAGA GCACCCCCAG CTCGACGTCC TCAGATAAGA AGAAGGATCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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nca-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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