gh13-2 cDNA ORF clone, Neurospora crassa OR74A

The following gh13-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gh13-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf12669 XM_958972.2
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Neurospora crassa OR74A alpha-amylase (gh13-2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf12669
    Clone ID Related Accession (Same CDS sequence) XM_958972.2
    Accession Version XM_958972.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product alpha-amylase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958972.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGGCCCG AAAGACAACT AAGCGTTGTC CTCGGACTGC TACTCTCAGC GGCGGTCCAA 
    GTGGGAGCTC TTACGTCTTC AGAATGGCGC AAGCAGTCCA TATATCAGGT CGTGACGGAT
    CGTTTTGCTC GAAGCGATCT GTCAACCAGT GCGCCATGTG ACACATCCCA GCAGGTATAC
    TGCGGCGGGT CATGGCAGGG ACTGATCTCG AAGCTGGACT ATATTCAGGG AATGGGATTC
    ACTGCTGTCT GGATATCCCC AATCGTGAAG CAGATTGACG GAGTTACCAA AGATGGGTCT
    TCTTATCATG GGTACTGGGC GTCAGATATT TGGTCCTTGA ATCCTTCATT TGGCACCGCA
    GACAACCTCA AGGGGCTCTC CAAAGCGCTT CATGACCGCG GCATGTACCT CATGGTGGAT
    GTTGTCACCA ACCACATGGC GTATTTGGGC TGCCGTTCGT GCATCAACTA CCAAGGTCTC
    AATCCCTTCT CTTCCCAATC TTACTATCAC TCTCCGTGCA CCATCAACTA CGATAACCAA
    ACCTCCATCG AAACATGCTG GCAAGGAAGT GATGTTGTCA GTTTACCGGA CCTCAAAACC
    GAAACCGACG ACGTCCGTAA GATATGGCAC GACTGGGTGT CCAAGATCGT GTCAACGTAC
    AGTATCGATG GCCTTCGTAT CGATAGTGTT AAGCACGTTG AAAAGTCCTT CTGGCCAGGA
    TTCGAGTCCG CTGCCGGCGT GTTCGCCATT GGAGAGGTGT TCCAAGGAGA CCCGGCGTAT
    CTGGCACCGT ACCAGAGCTA CATCAGCGGC GTCTTGGATT ATGCAAGCTA TTATTGGATA
    GTAAATGCGT TCCAGTCAAC AAGCGGCAAC ATCACTGCGC TGGCTAACGG TATGAACATA
    CTTAAAGCTG CTGCGATTGA CCTCAGTCTC TACGGGTCGT TCCTCGAAAA CCATGACCAG
    GCTCGCTTTG CGCACAAAAC TAGTGATATG GTCTTAGCAA AGAACGCAAT TGCCTTTACG
    ATGCTCAAGG ACGGCATCCC GATCATTTAT CAGGGACAGG AACAGCATTA TGCGGGCAGT
    GATACTCCCT ACAACCGAGA GGCCCTCTGG CTTTCCGGCT TTTCAACTAA CTCAGAGTTG
    TACACGTGGA TTAAACGGCT GAATCAGCTT CGTACCCATG CTATCAAAAA AGACGACGGC
    TACCTGACAT ACCGTGCATG GCCCATCTAC TCCGATAGCC ACACTATTGC CATGCGCAAA
    GGTTCATCCA ACAAACAAGT TGTGGGCGTC TTCACCAATG TCGGAGCGTC GTCCTCCACC
    GCCGTCACTC TGTCTTCGTT GGCGTCAGGA TTCAGTGCAG ATGAACCTGT CACCGACGTC
    GTGAGTTGCG ATAGCTTCAT CACCGACTCT GGAGGCAGCA TAACCATCAC ACTTTTTGGG
    GGGCTCCCAA GGATCTTATA CTCCACAACG CTTCTCAACG GCAGCGATAT TTGTTCCTCA
    CGGTCAGCTC CCACTTCGAC CACCAGCACA AATTCCGGAG TTCCTACATC GATTTCGTCG
    GCGGCATCGA CATCTTGCAC TGCCACTGCA GTACCCATCA CCTTCGAAGC GCTGGTTAGC
    ACGTCCTATG GTGAGACAGT TAAGCTATTG GGCAACACGA CTACCCTCGG TAGCTGGAAC
    ACCGCCAACG CTGTGACGCT AAGCGCGTCA AAGTACACCA GCAGCAACCC GCTGTGGTAC
    GTATCCTTCG ACCTTGCGCC GGGAAATGTC ATACTATACA AATTTATCAA GGTAAACAGT
    TCAGGGACTG TTTCTTGGGA GAGTGATCCC AATCACAAAT ATACGGTGCC TTGCGCAGCC
    GCCACGGTAT CTTCAACTTG GAGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_964065.2
    CDS464..2353
    Translation

    Target ORF information:

    RefSeq Version XM_958972.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A alpha-amylase (gh13-2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_958972.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGGCCCG AAAGACAACT AAGCGTTGTC CTCGGACTGC TACTCTCAGC GGCGGTCCAA 
    GTGGGAGCTC TTACGTCTTC AGAATGGCGC AAGCAGTCCA TATATCAGGT CGTGACGGAT
    CGTTTTGCTC GAAGCGATCT GTCAACCAGT GCGCCATGTG ACACATCCCA GCAGGTATAC
    TGCGGCGGGT CATGGCAGGG ACTGATCTCG AAGCTGGACT ATATTCAGGG AATGGGATTC
    ACTGCTGTCT GGATATCCCC AATCGTGAAG CAGATTGACG GAGTTACCAA AGATGGGTCT
    TCTTATCATG GGTACTGGGC GTCAGATATT TGGTCCTTGA ATCCTTCATT TGGCACCGCA
    GACAACCTCA AGGGGCTCTC CAAAGCGCTT CATGACCGCG GCATGTACCT CATGGTGGAT
    GTTGTCACCA ACCACATGGC GTATTTGGGC TGCCGTTCGT GCATCAACTA CCAAGGTCTC
    AATCCCTTCT CTTCCCAATC TTACTATCAC TCTCCGTGCA CCATCAACTA CGATAACCAA
    ACCTCCATCG AAACATGCTG GCAAGGAAGT GATGTTGTCA GTTTACCGGA CCTCAAAACC
    GAAACCGACG ACGTCCGTAA GATATGGCAC GACTGGGTGT CCAAGATCGT GTCAACGTAC
    AGTATCGATG GCCTTCGTAT CGATAGTGTT AAGCACGTTG AAAAGTCCTT CTGGCCAGGA
    TTCGAGTCCG CTGCCGGCGT GTTCGCCATT GGAGAGGTGT TCCAAGGAGA CCCGGCGTAT
    CTGGCACCGT ACCAGAGCTA CATCAGCGGC GTCTTGGATT ATGCAAGCTA TTATTGGATA
    GTAAATGCGT TCCAGTCAAC AAGCGGCAAC ATCACTGCGC TGGCTAACGG TATGAACATA
    CTTAAAGCTG CTGCGATTGA CCTCAGTCTC TACGGGTCGT TCCTCGAAAA CCATGACCAG
    GCTCGCTTTG CGCACAAAAC TAGTGATATG GTCTTAGCAA AGAACGCAAT TGCCTTTACG
    ATGCTCAAGG ACGGCATCCC GATCATTTAT CAGGGACAGG AACAGCATTA TGCGGGCAGT
    GATACTCCCT ACAACCGAGA GGCCCTCTGG CTTTCCGGCT TTTCAACTAA CTCAGAGTTG
    TACACGTGGA TTAAACGGCT GAATCAGCTT CGTACCCATG CTATCAAAAA AGACGACGGC
    TACCTGACAT ACCGTGCATG GCCCATCTAC TCCGATAGCC ACACTATTGC CATGCGCAAA
    GGTTCATCCA ACAAACAAGT TGTGGGCGTC TTCACCAATG TCGGAGCGTC GTCCTCCACC
    GCCGTCACTC TGTCTTCGTT GGCGTCAGGA TTCAGTGCAG ATGAACCTGT CACCGACGTC
    GTGAGTTGCG ATAGCTTCAT CACCGACTCT GGAGGCAGCA TAACCATCAC ACTTTTTGGG
    GGGCTCCCAA GGATCTTATA CTCCACAACG CTTCTCAACG GCAGCGATAT TTGTTCCTCA
    CGGTCAGCTC CCACTTCGAC CACCAGCACA AATTCCGGAG TTCCTACATC GATTTCGTCG
    GCGGCATCGA CATCTTGCAC TGCCACTGCA GTACCCATCA CCTTCGAAGC GCTGGTTAGC
    ACGTCCTATG GTGAGACAGT TAAGCTATTG GGCAACACGA CTACCCTCGG TAGCTGGAAC
    ACCGCCAACG CTGTGACGCT AAGCGCGTCA AAGTACACCA GCAGCAACCC GCTGTGGTAC
    GTATCCTTCG ACCTTGCGCC GGGAAATGTC ATACTATACA AATTTATCAA GGTAAACAGT
    TCAGGGACTG TTTCTTGGGA GAGTGATCCC AATCACAAAT ATACGGTGCC TTGCGCAGCC
    GCCACGGTAT CTTCAACTTG GAGGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B