rcm-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	rcm-1
Entrez Gene ID	3879465
Full Name	regulator of conidiation and morphology-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395299.1	XP_011393601.1	RCM-1, variant
XM_011395298.1	XP_011393600.1	RCM-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate that protein kinase A regulates frq transcription by inhibiting RCM-1 activity through RCM-1 phosphorylation.
Title: Role for Protein Kinase A in the Neurospora Circadian Clock by Regulating White Collar-Independent frequency Transcription through Phosphorylation of RCM-1.

Glycogen content is misregulated in rcm-1(RIP) strain, and the glycogen synthase phosphorylation is decreased, showing that RCM-1 is involved in glycogen accumulation and in the regulation of GSN activity by phosphorylation.
Title: Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator.

Forkhead associated protein (ham-4)of Neurospora crassa is required for hyphal fusion.
Title: Genes encoding a striatin-like protein (ham-3) and a forkhead associated protein (ham-4) are required for hyphal fusion in Neurospora crassa.

Rcm-1(NCU06842), rco-1(NCU06205) and ham-5(NCU11925) were required for hyphal fusion.
Title: The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa.

The following rcm-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rcm-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf01909	XM_011395298.1 Latest version!	Neurospora crassa OR74A RCM-1 (rcm-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
ONf01909	XM_011395299.1 Latest version!	Neurospora crassa OR74A RCM-1, variant (rcm-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rcm-1 ( XM_011395299.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393601.1 Neurospora crassa OR74A RCM-1, variant (rcm-1), mRNA.

Price & Availability↑

CloneID	ONf01909
Clone ID Related Accession (Same CDS sequence)	XM_011395299.1 , XM_011395298.1
Accession Version	XM_011395299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	RCM-1, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGCAAACC ACCACCCTTC CCCTACGATG CAGATGCAAA TGCATCACGG CCCGCCAGGG 
CCCCCAGGTC CACCGCCGGC GTCGATTCCT TCATGGAATC AACAGCGGCA GGCCTTTATG 
AGCTTGACCG AGAATGTGTG GATCGGAATC GGTTCCGTGT CCGAGCTCAT GGGGAACCAC 
AACGAGGCAC TAGAGGCTTA TGAAAGGGCT CTTGCAGCCA ACCCCAACTC TGTGACTGCT 
ATGAATGCCG CCAGTCTCGT CCTTAGGACG CGCGAGGACT TCCCCAAGGC GTCGGAATAT 
CTGCAGAGGA TCCTAAAGAT CGAGCCTGCA AACGGTGAAG CATGGGGCAG CCTCGGACAT 
TGCTTTCTCA TGATGGAGGA CCTCCAACAG GCATATGCTG CGTACCAAGC GGCCTTGGTT 
AACCTTCCCA ACCCAAAGGA ACCGAGGCTT TGGTATGGCA TAGGTATTCT TTATGATCGC 
TATGGCTCTT TGGACCATGC AGAGGAAGCA TTCTCGCAGG TCATGGCCAT GGACCCCAAC 
TTCGACAAGG CTCATGAGAT ATATTTCCGT CTCGGCATAA TCTACAAGCA ACAGCATAAG 
TACCAGCAAT CGCTTGATTG CTTCAGATAC ATTGTCAACT CTCCTCCTAC TCCTTTGACC 
GAAGAGGATA TCTGGTTCCA GATTGGTCAC GTCCATGAAC AGCAAAAAGA CTATGATGGT 
GCCAAGCAAG CCTACGAGCG AGTGCTGCAA CGTGATCCAA AGCACGCAAA GGTCCTTCAG 
CAGTTGGGCT GGCTGCATCA CCAGCAAAGT AATTCAGTCG CCAGCCAGGA AAAGGCAATC 
GAGTACCTGA ATCAGTCCGT TGCAGCTGAC CAAACCGATG CCCAAAGCTG GTACTTGCTC 
GGTCGTTGTT ACATGCAACT TCAAAAGTAC CCTAAGGCAT ACGAGGCTTA TCAGCAAGCT 
GTTTATAGGG ATGGCAGGAA CCCTACGTTC TGGTGCTCGA TTGGTGTGTT ATACTACCAG 
ATCAACCAGT ATCGTGACGC CTTGGACGCC TATTCCAGGG CCATCAGGCT CAACCCCTTC 
ATTTCTGAAG TTTGGTATGA CCTTGGCACT TTGTATGAGT CTTGCAACAA CCAGATCAGC 
GATGCTCTGG ACGCTTATCA ACGTGCCGCC GAACTGGATC CCAACAACCC GCATATCAAG 
ACCAGATTGC AGCTCCTCCG AAGTGGGCAA GCTAATGGCG GCGCACCCCC GGGGTCAGTC 
CCGATGCCCA CTGATATCCA CCCCCAGACG TATAATGCAT CCGGTGCGGT TGGTCCTCCG 
GGCCCTCAGT GGGCGGGCTC AGGCTCAGGC CAGCCTCCTC ATCAACCGCC TCAGCCGATG 
CACAACGGTG GCCCTGGCCC GGGCCAAGGG GCCAACTCGT GGGGTGGAAG AATTTCCGAC 
ATTAACCCTC CTCCCCAGCC GCCGAACCCA TATGCCTCGG GTCAAGACCG GGAGCCCTTT 
CGTGGTCCCG CTCCGCCGCT TCCCAGACAG CCCAGCCCGC GACAGGAGCA GCAAATGAGG 
CCTTATCAGG AGGCCAGGGC GCCCGAGCCG CTCAGAAGAG GACCTACCCC TCCCCAGGCT 
CATTACGCCC CTCCACCCCC TCCACCTCCG CAACAACCGC ACCAGCCGCA CCCGCAGCAA 
CAGCTTCAGC AAGGACCTCA ACCTACTCGG GAAGGGGGTA GTGGTACTAG AGTGAGGAAC 
CCGAACTACG CTAATCCCCA GAACGTGGTC CCTTCCAATT CTGGTCCTGG CCCAAACGGC 
CCTCCTCCTC CAAACGCAAT GATGCATTTT AACAACAGTC CGAGGACTGA TGGAAGACCA 
CCCCACATGC ACGAGAACCG CATGCCGTCT CCCAAGTCCG CCTATCCTCA GCATCAGCCG 
CCGTACCCTC CGCATGGTGA GCAGGGCGGT CCTGGTGGCC CTGAGCCAGG CCCTCCTCAC 
CCCCCTCAGT CAGGTATGGC TGGTGAACCT CCTCATCAGA GGGAGCATGA TCCCCGCCCT 
CCTTCGGTTG GCCCCAAGAG AATGCGTGAG TGGGAAGACG ACCGGGAGGT GAAGAAGCCT 
GCGACGGAGG AGACTCGCGT CCGTATGGAT GACCATAGGC ACCGCCGCCC CTCGATGACC 
CCGCCCCGAA TGGAGCCGCC CTATGCTCGT CGCAACTCGT CGGAGGCTCG TCGCTTCGAC 
GAGCGTCGTA TGGAAGATTC CCGTAGGGTT GAGGAGCAGC GCCGGGCTGA GGAACAACGT 
CGCATGGAGG ACATGCGCCG GGCTGAGGAG CAGCGGCACC AGAACGAGGG ATACCATCCT 
TCGGAGGCCG CTCATCATCC CCAGTCTCAT TCCGCCCCTG CCCATTTGCC GCCGATGCAG 
CAAGGTTCGG CTCCGATGCA GAACCTCATC CACGAGCAAG GCCACGGACC CCAGCAGCCA 
GTCCCCGGAC CTCAGCAGCA AGGCCCAGGC CCAGCACATC AGCCCGCCCC CGAAGACCGA 
CGGATGGACC ATCCTCCAGC CCAGCACCCC CCCATCGTCA ATGAGCCTGA GCGTGCGGCC 
CGTAAAATGG ACGTTGACGA GGACTATGAC GATAGCGGAG AGGAGGACAA GAAGGGCGGA 
ATTATTCCCG GTCCTTCCTC CGGATCCGGC CCTGCAGCAA ACGAGTCCAA GAACGGAGCC 
TCAACGAGCG GTAGCTTCAA CGGCATCATG GGACAAAAGT CAGAAAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393601.1
CDS	690..3443
Translation	MANHHPSPTMQMQMHHGPPGPPGPPPASIPSWNQQRQAFMSLTENVWIGIGSVSELMGNHNEALEAYERALAANPNSVTAMNAASLVLRTREDFPKASEYLQRILKIEPANGEAWGSLGHCFLMMEDLQQAYAAYQAALVNLPNPKEPRLWYGIGILYDRYGSLDHAEEAFSQVMAMDPNFDKAHEIYFRLGIIYKQQHKYQQSLDCFRYIVNSPPTPLTEEDIWFQIGHVHEQQKDYDGAKQAYERVLQRDPKHAKVLQQLGWLHHQQSNSVASQEKAIEYLNQSVAADQTDAQSWYLLGRCYMQLQKYPKAYEAYQQAVYRDGRNPTFWCSIGVLYYQINQYRDALDAYSRAIRLNPFISEVWYDLGTLYESCNNQISDALDAYQRAAELDPNNPHIKTRLQLLRSGQANGGAPPGSVPMPTDIHPQTYNASGAVGPPGPQWAGSGSGQPPHQPPQPMHNGGPGPGQGANSWGGRISDINPPPQPPNPYASGQDREPFRGPAPPLPRQPSPRQEQQMRPYQEARAPEPLRRGPTPPQAHYAPPPPPPPQQPHQPHPQQQLQQGPQPTREGGSGTRVRNPNYANPQNVVPSNSGPGPNGPPPPNAMMHFNNSPRTDGRPPHMHENRMPSPKSAYPQHQPPYPPHGEQGGPGGPEPGPPHPPQSGMAGEPPHQREHDPRPPSVGPKRMREWEDDREVKKPATEETRVRMDDHRHRRPSMTPPRMEPPYARRNSSEARRFDERRMEDSRRVEEQRRAEEQRRMEDMRRAEEQRHQNEGYHPSEAAHHPQSHSAPAHLPPMQQGSAPMQNLIHEQGHGPQQPVPGPQQQGPGPAHQPAPEDRRMDHPPAQHPPIVNEPERAARKMDVDEDYDDSGEEDKKGGIIPGPSSGSGPAANESKNGASTSGSFNGIMGQKSESN

Target ORF information:

RefSeq Version	XM_011395299.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A RCM-1, variant (rcm-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395299.1

ORF Insert Sequence:

ATGGCAAACC ACCACCCTTC CCCTACGATG CAGATGCAAA TGCATCACGG CCCGCCAGGG 
CCCCCAGGTC CACCGCCGGC GTCGATTCCT TCATGGAATC AACAGCGGCA GGCCTTTATG 
AGCTTGACCG AGAATGTGTG GATCGGAATC GGTTCCGTGT CCGAGCTCAT GGGGAACCAC 
AACGAGGCAC TAGAGGCTTA TGAAAGGGCT CTTGCAGCCA ACCCCAACTC TGTGACTGCT 
ATGAATGCCG CCAGTCTCGT CCTTAGGACG CGCGAGGACT TCCCCAAGGC GTCGGAATAT 
CTGCAGAGGA TCCTAAAGAT CGAGCCTGCA AACGGTGAAG CATGGGGCAG CCTCGGACAT 
TGCTTTCTCA TGATGGAGGA CCTCCAACAG GCATATGCTG CGTACCAAGC GGCCTTGGTT 
AACCTTCCCA ACCCAAAGGA ACCGAGGCTT TGGTATGGCA TAGGTATTCT TTATGATCGC 
TATGGCTCTT TGGACCATGC AGAGGAAGCA TTCTCGCAGG TCATGGCCAT GGACCCCAAC 
TTCGACAAGG CTCATGAGAT ATATTTCCGT CTCGGCATAA TCTACAAGCA ACAGCATAAG 
TACCAGCAAT CGCTTGATTG CTTCAGATAC ATTGTCAACT CTCCTCCTAC TCCTTTGACC 
GAAGAGGATA TCTGGTTCCA GATTGGTCAC GTCCATGAAC AGCAAAAAGA CTATGATGGT 
GCCAAGCAAG CCTACGAGCG AGTGCTGCAA CGTGATCCAA AGCACGCAAA GGTCCTTCAG 
CAGTTGGGCT GGCTGCATCA CCAGCAAAGT AATTCAGTCG CCAGCCAGGA AAAGGCAATC 
GAGTACCTGA ATCAGTCCGT TGCAGCTGAC CAAACCGATG CCCAAAGCTG GTACTTGCTC 
GGTCGTTGTT ACATGCAACT TCAAAAGTAC CCTAAGGCAT ACGAGGCTTA TCAGCAAGCT 
GTTTATAGGG ATGGCAGGAA CCCTACGTTC TGGTGCTCGA TTGGTGTGTT ATACTACCAG 
ATCAACCAGT ATCGTGACGC CTTGGACGCC TATTCCAGGG CCATCAGGCT CAACCCCTTC 
ATTTCTGAAG TTTGGTATGA CCTTGGCACT TTGTATGAGT CTTGCAACAA CCAGATCAGC 
GATGCTCTGG ACGCTTATCA ACGTGCCGCC GAACTGGATC CCAACAACCC GCATATCAAG 
ACCAGATTGC AGCTCCTCCG AAGTGGGCAA GCTAATGGCG GCGCACCCCC GGGGTCAGTC 
CCGATGCCCA CTGATATCCA CCCCCAGACG TATAATGCAT CCGGTGCGGT TGGTCCTCCG 
GGCCCTCAGT GGGCGGGCTC AGGCTCAGGC CAGCCTCCTC ATCAACCGCC TCAGCCGATG 
CACAACGGTG GCCCTGGCCC GGGCCAAGGG GCCAACTCGT GGGGTGGAAG AATTTCCGAC 
ATTAACCCTC CTCCCCAGCC GCCGAACCCA TATGCCTCGG GTCAAGACCG GGAGCCCTTT 
CGTGGTCCCG CTCCGCCGCT TCCCAGACAG CCCAGCCCGC GACAGGAGCA GCAAATGAGG 
CCTTATCAGG AGGCCAGGGC GCCCGAGCCG CTCAGAAGAG GACCTACCCC TCCCCAGGCT 
CATTACGCCC CTCCACCCCC TCCACCTCCG CAACAACCGC ACCAGCCGCA CCCGCAGCAA 
CAGCTTCAGC AAGGACCTCA ACCTACTCGG GAAGGGGGTA GTGGTACTAG AGTGAGGAAC 
CCGAACTACG CTAATCCCCA GAACGTGGTC CCTTCCAATT CTGGTCCTGG CCCAAACGGC 
CCTCCTCCTC CAAACGCAAT GATGCATTTT AACAACAGTC CGAGGACTGA TGGAAGACCA 
CCCCACATGC ACGAGAACCG CATGCCGTCT CCCAAGTCCG CCTATCCTCA GCATCAGCCG 
CCGTACCCTC CGCATGGTGA GCAGGGCGGT CCTGGTGGCC CTGAGCCAGG CCCTCCTCAC 
CCCCCTCAGT CAGGTATGGC TGGTGAACCT CCTCATCAGA GGGAGCATGA TCCCCGCCCT 
CCTTCGGTTG GCCCCAAGAG AATGCGTGAG TGGGAAGACG ACCGGGAGGT GAAGAAGCCT 
GCGACGGAGG AGACTCGCGT CCGTATGGAT GACCATAGGC ACCGCCGCCC CTCGATGACC 
CCGCCCCGAA TGGAGCCGCC CTATGCTCGT CGCAACTCGT CGGAGGCTCG TCGCTTCGAC 
GAGCGTCGTA TGGAAGATTC CCGTAGGGTT GAGGAGCAGC GCCGGGCTGA GGAACAACGT 
CGCATGGAGG ACATGCGCCG GGCTGAGGAG CAGCGGCACC AGAACGAGGG ATACCATCCT 
TCGGAGGCCG CTCATCATCC CCAGTCTCAT TCCGCCCCTG CCCATTTGCC GCCGATGCAG 
CAAGGTTCGG CTCCGATGCA GAACCTCATC CACGAGCAAG GCCACGGACC CCAGCAGCCA 
GTCCCCGGAC CTCAGCAGCA AGGCCCAGGC CCAGCACATC AGCCCGCCCC CGAAGACCGA 
CGGATGGACC ATCCTCCAGC CCAGCACCCC CCCATCGTCA ATGAGCCTGA GCGTGCGGCC 
CGTAAAATGG ACGTTGACGA GGACTATGAC GATAGCGGAG AGGAGGACAA GAAGGGCGGA 
ATTATTCCCG GTCCTTCCTC CGGATCCGGC CCTGCAGCAA ACGAGTCCAA GAACGGAGCC 
TCAACGAGCG GTAGCTTCAA CGGCATCATG GGACAAAAGT CAGAAAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rcm-1 ( XM_011395298.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393600.1 Neurospora crassa OR74A RCM-1 (rcm-1), mRNA.

Price & Availability↑

CloneID	ONf01909
Clone ID Related Accession (Same CDS sequence)	XM_011395299.1 , XM_011395298.1
Accession Version	XM_011395298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	RCM-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGCAAACC ACCACCCTTC CCCTACGATG CAGATGCAAA TGCATCACGG CCCGCCAGGG 
CCCCCAGGTC CACCGCCGGC GTCGATTCCT TCATGGAATC AACAGCGGCA GGCCTTTATG 
AGCTTGACCG AGAATGTGTG GATCGGAATC GGTTCCGTGT CCGAGCTCAT GGGGAACCAC 
AACGAGGCAC TAGAGGCTTA TGAAAGGGCT CTTGCAGCCA ACCCCAACTC TGTGACTGCT 
ATGAATGCCG CCAGTCTCGT CCTTAGGACG CGCGAGGACT TCCCCAAGGC GTCGGAATAT 
CTGCAGAGGA TCCTAAAGAT CGAGCCTGCA AACGGTGAAG CATGGGGCAG CCTCGGACAT 
TGCTTTCTCA TGATGGAGGA CCTCCAACAG GCATATGCTG CGTACCAAGC GGCCTTGGTT 
AACCTTCCCA ACCCAAAGGA ACCGAGGCTT TGGTATGGCA TAGGTATTCT TTATGATCGC 
TATGGCTCTT TGGACCATGC AGAGGAAGCA TTCTCGCAGG TCATGGCCAT GGACCCCAAC 
TTCGACAAGG CTCATGAGAT ATATTTCCGT CTCGGCATAA TCTACAAGCA ACAGCATAAG 
TACCAGCAAT CGCTTGATTG CTTCAGATAC ATTGTCAACT CTCCTCCTAC TCCTTTGACC 
GAAGAGGATA TCTGGTTCCA GATTGGTCAC GTCCATGAAC AGCAAAAAGA CTATGATGGT 
GCCAAGCAAG CCTACGAGCG AGTGCTGCAA CGTGATCCAA AGCACGCAAA GGTCCTTCAG 
CAGTTGGGCT GGCTGCATCA CCAGCAAAGT AATTCAGTCG CCAGCCAGGA AAAGGCAATC 
GAGTACCTGA ATCAGTCCGT TGCAGCTGAC CAAACCGATG CCCAAAGCTG GTACTTGCTC 
GGTCGTTGTT ACATGCAACT TCAAAAGTAC CCTAAGGCAT ACGAGGCTTA TCAGCAAGCT 
GTTTATAGGG ATGGCAGGAA CCCTACGTTC TGGTGCTCGA TTGGTGTGTT ATACTACCAG 
ATCAACCAGT ATCGTGACGC CTTGGACGCC TATTCCAGGG CCATCAGGCT CAACCCCTTC 
ATTTCTGAAG TTTGGTATGA CCTTGGCACT TTGTATGAGT CTTGCAACAA CCAGATCAGC 
GATGCTCTGG ACGCTTATCA ACGTGCCGCC GAACTGGATC CCAACAACCC GCATATCAAG 
ACCAGATTGC AGCTCCTCCG AAGTGGGCAA GCTAATGGCG GCGCACCCCC GGGGTCAGTC 
CCGATGCCCA CTGATATCCA CCCCCAGACG TATAATGCAT CCGGTGCGGT TGGTCCTCCG 
GGCCCTCAGT GGGCGGGCTC AGGCTCAGGC CAGCCTCCTC ATCAACCGCC TCAGCCGATG 
CACAACGGTG GCCCTGGCCC GGGCCAAGGG GCCAACTCGT GGGGTGGAAG AATTTCCGAC 
ATTAACCCTC CTCCCCAGCC GCCGAACCCA TATGCCTCGG GTCAAGACCG GGAGCCCTTT 
CGTGGTCCCG CTCCGCCGCT TCCCAGACAG CCCAGCCCGC GACAGGAGCA GCAAATGAGG 
CCTTATCAGG AGGCCAGGGC GCCCGAGCCG CTCAGAAGAG GACCTACCCC TCCCCAGGCT 
CATTACGCCC CTCCACCCCC TCCACCTCCG CAACAACCGC ACCAGCCGCA CCCGCAGCAA 
CAGCTTCAGC AAGGACCTCA ACCTACTCGG GAAGGGGGTA GTGGTACTAG AGTGAGGAAC 
CCGAACTACG CTAATCCCCA GAACGTGGTC CCTTCCAATT CTGGTCCTGG CCCAAACGGC 
CCTCCTCCTC CAAACGCAAT GATGCATTTT AACAACAGTC CGAGGACTGA TGGAAGACCA 
CCCCACATGC ACGAGAACCG CATGCCGTCT CCCAAGTCCG CCTATCCTCA GCATCAGCCG 
CCGTACCCTC CGCATGGTGA GCAGGGCGGT CCTGGTGGCC CTGAGCCAGG CCCTCCTCAC 
CCCCCTCAGT CAGGTATGGC TGGTGAACCT CCTCATCAGA GGGAGCATGA TCCCCGCCCT 
CCTTCGGTTG GCCCCAAGAG AATGCGTGAG TGGGAAGACG ACCGGGAGGT GAAGAAGCCT 
GCGACGGAGG AGACTCGCGT CCGTATGGAT GACCATAGGC ACCGCCGCCC CTCGATGACC 
CCGCCCCGAA TGGAGCCGCC CTATGCTCGT CGCAACTCGT CGGAGGCTCG TCGCTTCGAC 
GAGCGTCGTA TGGAAGATTC CCGTAGGGTT GAGGAGCAGC GCCGGGCTGA GGAACAACGT 
CGCATGGAGG ACATGCGCCG GGCTGAGGAG CAGCGGCACC AGAACGAGGG ATACCATCCT 
TCGGAGGCCG CTCATCATCC CCAGTCTCAT TCCGCCCCTG CCCATTTGCC GCCGATGCAG 
CAAGGTTCGG CTCCGATGCA GAACCTCATC CACGAGCAAG GCCACGGACC CCAGCAGCCA 
GTCCCCGGAC CTCAGCAGCA AGGCCCAGGC CCAGCACATC AGCCCGCCCC CGAAGACCGA 
CGGATGGACC ATCCTCCAGC CCAGCACCCC CCCATCGTCA ATGAGCCTGA GCGTGCGGCC 
CGTAAAATGG ACGTTGACGA GGACTATGAC GATAGCGGAG AGGAGGACAA GAAGGGCGGA 
ATTATTCCCG GTCCTTCCTC CGGATCCGGC CCTGCAGCAA ACGAGTCCAA GAACGGAGCC 
TCAACGAGCG GTAGCTTCAA CGGCATCATG GGACAAAAGT CAGAAAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393600.1
CDS	697..3450
Translation	MANHHPSPTMQMQMHHGPPGPPGPPPASIPSWNQQRQAFMSLTENVWIGIGSVSELMGNHNEALEAYERALAANPNSVTAMNAASLVLRTREDFPKASEYLQRILKIEPANGEAWGSLGHCFLMMEDLQQAYAAYQAALVNLPNPKEPRLWYGIGILYDRYGSLDHAEEAFSQVMAMDPNFDKAHEIYFRLGIIYKQQHKYQQSLDCFRYIVNSPPTPLTEEDIWFQIGHVHEQQKDYDGAKQAYERVLQRDPKHAKVLQQLGWLHHQQSNSVASQEKAIEYLNQSVAADQTDAQSWYLLGRCYMQLQKYPKAYEAYQQAVYRDGRNPTFWCSIGVLYYQINQYRDALDAYSRAIRLNPFISEVWYDLGTLYESCNNQISDALDAYQRAAELDPNNPHIKTRLQLLRSGQANGGAPPGSVPMPTDIHPQTYNASGAVGPPGPQWAGSGSGQPPHQPPQPMHNGGPGPGQGANSWGGRISDINPPPQPPNPYASGQDREPFRGPAPPLPRQPSPRQEQQMRPYQEARAPEPLRRGPTPPQAHYAPPPPPPPQQPHQPHPQQQLQQGPQPTREGGSGTRVRNPNYANPQNVVPSNSGPGPNGPPPPNAMMHFNNSPRTDGRPPHMHENRMPSPKSAYPQHQPPYPPHGEQGGPGGPEPGPPHPPQSGMAGEPPHQREHDPRPPSVGPKRMREWEDDREVKKPATEETRVRMDDHRHRRPSMTPPRMEPPYARRNSSEARRFDERRMEDSRRVEEQRRAEEQRRMEDMRRAEEQRHQNEGYHPSEAAHHPQSHSAPAHLPPMQQGSAPMQNLIHEQGHGPQQPVPGPQQQGPGPAHQPAPEDRRMDHPPAQHPPIVNEPERAARKMDVDEDYDDSGEEDKKGGIIPGPSSGSGPAANESKNGASTSGSFNGIMGQKSESN

Target ORF information:

RefSeq Version	XM_011395298.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A RCM-1 (rcm-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395298.1

ORF Insert Sequence:

ATGGCAAACC ACCACCCTTC CCCTACGATG CAGATGCAAA TGCATCACGG CCCGCCAGGG 
CCCCCAGGTC CACCGCCGGC GTCGATTCCT TCATGGAATC AACAGCGGCA GGCCTTTATG 
AGCTTGACCG AGAATGTGTG GATCGGAATC GGTTCCGTGT CCGAGCTCAT GGGGAACCAC 
AACGAGGCAC TAGAGGCTTA TGAAAGGGCT CTTGCAGCCA ACCCCAACTC TGTGACTGCT 
ATGAATGCCG CCAGTCTCGT CCTTAGGACG CGCGAGGACT TCCCCAAGGC GTCGGAATAT 
CTGCAGAGGA TCCTAAAGAT CGAGCCTGCA AACGGTGAAG CATGGGGCAG CCTCGGACAT 
TGCTTTCTCA TGATGGAGGA CCTCCAACAG GCATATGCTG CGTACCAAGC GGCCTTGGTT 
AACCTTCCCA ACCCAAAGGA ACCGAGGCTT TGGTATGGCA TAGGTATTCT TTATGATCGC 
TATGGCTCTT TGGACCATGC AGAGGAAGCA TTCTCGCAGG TCATGGCCAT GGACCCCAAC 
TTCGACAAGG CTCATGAGAT ATATTTCCGT CTCGGCATAA TCTACAAGCA ACAGCATAAG 
TACCAGCAAT CGCTTGATTG CTTCAGATAC ATTGTCAACT CTCCTCCTAC TCCTTTGACC 
GAAGAGGATA TCTGGTTCCA GATTGGTCAC GTCCATGAAC AGCAAAAAGA CTATGATGGT 
GCCAAGCAAG CCTACGAGCG AGTGCTGCAA CGTGATCCAA AGCACGCAAA GGTCCTTCAG 
CAGTTGGGCT GGCTGCATCA CCAGCAAAGT AATTCAGTCG CCAGCCAGGA AAAGGCAATC 
GAGTACCTGA ATCAGTCCGT TGCAGCTGAC CAAACCGATG CCCAAAGCTG GTACTTGCTC 
GGTCGTTGTT ACATGCAACT TCAAAAGTAC CCTAAGGCAT ACGAGGCTTA TCAGCAAGCT 
GTTTATAGGG ATGGCAGGAA CCCTACGTTC TGGTGCTCGA TTGGTGTGTT ATACTACCAG 
ATCAACCAGT ATCGTGACGC CTTGGACGCC TATTCCAGGG CCATCAGGCT CAACCCCTTC 
ATTTCTGAAG TTTGGTATGA CCTTGGCACT TTGTATGAGT CTTGCAACAA CCAGATCAGC 
GATGCTCTGG ACGCTTATCA ACGTGCCGCC GAACTGGATC CCAACAACCC GCATATCAAG 
ACCAGATTGC AGCTCCTCCG AAGTGGGCAA GCTAATGGCG GCGCACCCCC GGGGTCAGTC 
CCGATGCCCA CTGATATCCA CCCCCAGACG TATAATGCAT CCGGTGCGGT TGGTCCTCCG 
GGCCCTCAGT GGGCGGGCTC AGGCTCAGGC CAGCCTCCTC ATCAACCGCC TCAGCCGATG 
CACAACGGTG GCCCTGGCCC GGGCCAAGGG GCCAACTCGT GGGGTGGAAG AATTTCCGAC 
ATTAACCCTC CTCCCCAGCC GCCGAACCCA TATGCCTCGG GTCAAGACCG GGAGCCCTTT 
CGTGGTCCCG CTCCGCCGCT TCCCAGACAG CCCAGCCCGC GACAGGAGCA GCAAATGAGG 
CCTTATCAGG AGGCCAGGGC GCCCGAGCCG CTCAGAAGAG GACCTACCCC TCCCCAGGCT 
CATTACGCCC CTCCACCCCC TCCACCTCCG CAACAACCGC ACCAGCCGCA CCCGCAGCAA 
CAGCTTCAGC AAGGACCTCA ACCTACTCGG GAAGGGGGTA GTGGTACTAG AGTGAGGAAC 
CCGAACTACG CTAATCCCCA GAACGTGGTC CCTTCCAATT CTGGTCCTGG CCCAAACGGC 
CCTCCTCCTC CAAACGCAAT GATGCATTTT AACAACAGTC CGAGGACTGA TGGAAGACCA 
CCCCACATGC ACGAGAACCG CATGCCGTCT CCCAAGTCCG CCTATCCTCA GCATCAGCCG 
CCGTACCCTC CGCATGGTGA GCAGGGCGGT CCTGGTGGCC CTGAGCCAGG CCCTCCTCAC 
CCCCCTCAGT CAGGTATGGC TGGTGAACCT CCTCATCAGA GGGAGCATGA TCCCCGCCCT 
CCTTCGGTTG GCCCCAAGAG AATGCGTGAG TGGGAAGACG ACCGGGAGGT GAAGAAGCCT 
GCGACGGAGG AGACTCGCGT CCGTATGGAT GACCATAGGC ACCGCCGCCC CTCGATGACC 
CCGCCCCGAA TGGAGCCGCC CTATGCTCGT CGCAACTCGT CGGAGGCTCG TCGCTTCGAC 
GAGCGTCGTA TGGAAGATTC CCGTAGGGTT GAGGAGCAGC GCCGGGCTGA GGAACAACGT 
CGCATGGAGG ACATGCGCCG GGCTGAGGAG CAGCGGCACC AGAACGAGGG ATACCATCCT 
TCGGAGGCCG CTCATCATCC CCAGTCTCAT TCCGCCCCTG CCCATTTGCC GCCGATGCAG 
CAAGGTTCGG CTCCGATGCA GAACCTCATC CACGAGCAAG GCCACGGACC CCAGCAGCCA 
GTCCCCGGAC CTCAGCAGCA AGGCCCAGGC CCAGCACATC AGCCCGCCCC CGAAGACCGA 
CGGATGGACC ATCCTCCAGC CCAGCACCCC CCCATCGTCA ATGAGCCTGA GCGTGCGGCC 
CGTAAAATGG ACGTTGACGA GGACTATGAC GATAGCGGAG AGGAGGACAA GAAGGGCGGA 
ATTATTCCCG GTCCTTCCTC CGGATCCGGC CCTGCAGCAA ACGAGTCCAA GAACGGAGCC 
TCAACGAGCG GTAGCTTCAA CGGCATCATG GGACAAAAGT CAGAAAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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rcm-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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