ORF » Species Summary » Neurospora crassa OR74A » leu-1 cDNA ORF clone

leu-1 cDNA ORF clone, Neurospora crassa OR74A

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  • gRNAs

The following leu-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the leu-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf01730 XM_957990.2
Latest version!		 Neurospora crassa OR74A 3-isopropylmalate dehydrogenase (leu-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    leu-1 ( XM_957990.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_963083.1 Neurospora crassa OR74A 3-isopropylmalate dehydrogenase (leu-1), mRNA.
    Price & Availability↑
    CloneID ONf01730
    Clone ID Related Accession (Same CDS sequence) XM_957990.2
    Accession Version XM_957990.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product 3-isopropylmalate dehydrogenase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_957990.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCTACTC ATAACATTGT TGTGTTCGGT GGTGACCACT GCGGTCCCGA GGTTGTTCTC 
    GAGGCCATCA AGGTCCTCAA GGCGATCGAG ACCAACAGCC CTTCGGCTGG CAAGTTCAAC 
    CTCCAGAACC ACCTCCTTGG CGGTGCCTCC ATCGACAAGC ACAATGACCC CCTCACCGAT 
    GAGGCCCTCA ACGCCGCCAA GGCTGCCGAT GCCGTCCTTC TCGGTGCCAT TGGCGGTCCC 
    GAATGGGGCA CCTCTTCCAC CGTCCGCCCC GAGCAAGGTC TCCTGAAGCT CCGCAAGGAG 
    CTCGGCACCT ATGGCAACCT TCGCCCTTGC AACTTTGCTT CCGAGTCCCT CGTCGACAGC 
    TCTCCCCTCA AGGCCGAGGT CTGCCGCGGC ACTGACTTCA TTGTCGTCCG TGAGCTTACC 
    GGTGGTATCT ACTTTGGTGA CCGCACCGAG GATGACGGCT CCGGCTACGC CTGCGATACC 
    GAGCCCTACA GCCGCGCCGA GATCGTGCGC ATCGCCAGAC TCGCCGGCTT CCTCGCCCTG 
    GCCAAGAACC CTCCCGCCAA GGTCTGGTCT CTGGACAAGG CCAACGTGCT CGCCACCAGC 
    CGCCTCTGGC GCAAGACTGT GACCGACGTC ATTAGCAAGG AGTTCCCCCA GCTTCAGCTC 
    GAGCACCAGC TCATCGACAG CGCCGCCATG CTGCTCGTCA AGAACCCCCG TGCCCTCAAC 
    GGCGTCGTCA TTACCAGCAA CCTCTTTGGC GACATCATCT CGGACGAGGC CTCGGTCATC 
    CCCGGCTCCA TCGGCCTGCT CCCTTCCGCC AGCTTGGGCG GAATCCCCGA CGGCAAGGGC 
    AAGTGCAACG GCATTTACGA GCCCATCCAC GGCTCCGCTC CCGATATTTC GGGTAAGGGC 
    ATCGTCAACC CCGTCGGTAC CATTCTCTCC GTCGCCATGA TGCTCCGCTA CTCGCTCAAC 
    CTCCCCAAGG AGGCCGATGC CGTTGAGGCT GCTGTCAAGG CAGCCATTGA CAACGGTACC 
    AAGACCAAGG ACCTTGGCGG CAACGCTACT ACTTCGGATA TGGGTAACGC TGTAGTTGCT 
    GAGTTGGAGA AGATCCTTAA GGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_963083.1
    CDS247..1353
    Translation

    Target ORF information:

    RefSeq Version XM_957990.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A 3-isopropylmalate dehydrogenase (leu-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_957990.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCTACTC ATAACATTGT TGTGTTCGGT GGTGACCACT GCGGTCCCGA GGTTGTTCTC 
    GAGGCCATCA AGGTCCTCAA GGCGATCGAG ACCAACAGCC CTTCGGCTGG CAAGTTCAAC 
    CTCCAGAACC ACCTCCTTGG CGGTGCCTCC ATCGACAAGC ACAATGACCC CCTCACCGAT 
    GAGGCCCTCA ACGCCGCCAA GGCTGCCGAT GCCGTCCTTC TCGGTGCCAT TGGCGGTCCC 
    GAATGGGGCA CCTCTTCCAC CGTCCGCCCC GAGCAAGGTC TCCTGAAGCT CCGCAAGGAG 
    CTCGGCACCT ATGGCAACCT TCGCCCTTGC AACTTTGCTT CCGAGTCCCT CGTCGACAGC 
    TCTCCCCTCA AGGCCGAGGT CTGCCGCGGC ACTGACTTCA TTGTCGTCCG TGAGCTTACC 
    GGTGGTATCT ACTTTGGTGA CCGCACCGAG GATGACGGCT CCGGCTACGC CTGCGATACC 
    GAGCCCTACA GCCGCGCCGA GATCGTGCGC ATCGCCAGAC TCGCCGGCTT CCTCGCCCTG 
    GCCAAGAACC CTCCCGCCAA GGTCTGGTCT CTGGACAAGG CCAACGTGCT CGCCACCAGC 
    CGCCTCTGGC GCAAGACTGT GACCGACGTC ATTAGCAAGG AGTTCCCCCA GCTTCAGCTC 
    GAGCACCAGC TCATCGACAG CGCCGCCATG CTGCTCGTCA AGAACCCCCG TGCCCTCAAC 
    GGCGTCGTCA TTACCAGCAA CCTCTTTGGC GACATCATCT CGGACGAGGC CTCGGTCATC 
    CCCGGCTCCA TCGGCCTGCT CCCTTCCGCC AGCTTGGGCG GAATCCCCGA CGGCAAGGGC 
    AAGTGCAACG GCATTTACGA GCCCATCCAC GGCTCCGCTC CCGATATTTC GGGTAAGGGC 
    ATCGTCAACC CCGTCGGTAC CATTCTCTCC GTCGCCATGA TGCTCCGCTA CTCGCTCAAC 
    CTCCCCAAGG AGGCCGATGC CGTTGAGGCT GCTGTCAAGG CAGCCATTGA CAACGGTACC 
    AAGACCAAGG ACCTTGGCGG CAACGCTACT ACTTCGGATA TGGGTAACGC TGTAGTTGCT 
    GAGTTGGAGA AGATCCTTAA GGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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