NCU07710 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU07710
Entrez Gene ID	3878444
Full Name	vacuolar membrane-associated protein iml-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_957203.3	XP_962296.3	vacuolar membrane-associated protein iml-1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04160	XP_003719670.1
Neurospora crassa	NCU07710	XP_962296.2
Schizosaccharomyces pombe (fission yeast)	SPBC26H8.04c	NP_596645.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU07710 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU07710 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12055	XM_957203.3 Latest version!	Neurospora crassa OR74A vacuolar membrane-associated protein iml-1 (NCU07710), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU07710 ( XM_957203.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_962296.3 Neurospora crassa OR74A vacuolar membrane-associated protein iml-1 (NCU07710), mRNA.

Price & Availability↑

CloneID	ONf12055
Clone ID Related Accession (Same CDS sequence)	XM_957203.3
Accession Version	XM_957203.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	vacuolar membrane-associated protein iml-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_957203.2.

ATGGCGTCGC GCAATTTCTC TTCGGGGGTG CCGGGACATG TCAGGCCCCC CAACTGGTCG 
TCGCACCTCC GGCAGTTCAG CAGAAACAGC CTGGAGCGCA TCCCGGATAT GCCCCGGTCC 
CCGGACACTG TCTCTAGTAA CTCGACCATC CGTGACCGGC CCTCTTCATC CTCAAGACGT 
TCACGTTTCG AGAGGAGGTG GACCGTTACC GTCAACGAGT CCTTTGCTCG CGATGAAGTC 
TTGCTTAATC TTGATCTCAT AGGTGATGAG GCAACCCCAG GCAGCCTCAT GGCTATCGAC 
GTCTTCCGAC CTGATGCGGA GAAGCAAGCG CAAGGTTCTC ATCACCATCA CCACCACCAT 
CATCATCACC ATCATAAACA GCCTCCACCG GACCGCACAA AAGAGGCTGC AGAAAGTCAA 
CAGCGTTACA TCTTTGTCGT CAAGGACATG CCCAAGGAAC TCAAAGCCCG CTACCCGAGT 
GTTGAAGTCT ACGTCGCAAA GCACATTGCT GATGTCTTCG GCATGAGGAA GGGTTCGCAG 
GTTACCCTGA CCCCAATTGA TCATCATAAC CCAGCCATCG AGGCCTCGCA TGTCGAGCTT 
TGCTTTAGAG ACCAGTACAT GTCGCGCTCC GACATGTGGC AGTTGGCTGT TCGTGAGCTT 
TCAGAGAAGA CTGTCTACAA GGGCCAAATG GTTCTGTTCA TGGGCACTCT CAAGGCTCAG 
GTTACCGCTG TCTATGTTGA GGGCCGTAAG GTGCCATCCG CATTCTTTGG CCACAATACA 
AAACCCATCT TCCGAAGCGA GTCTGCTCGA TACGTGCTTT TCATCCAAAT GGCGCGCGAA 
ATGTGGGACT TTGATTCAGA TGGCTCTGGT GAGATCATGT TCAACAAGGT TGTGAACGGT 
TTTTTGCCAG CTCTGTTCAA GAAATGGGCG TCACTTAAAG TCAGGCACCT TGTCAGTATT 
GTTCTCTTTG CTCGAGTTGA GTATGACACA GGCATATCCA CCGAGTTGGG CAACCCGGAC 
GTCCAAAATG ACTACTACAC TGGTGTCCAG TCGTCTGGCG ATCGTCGCCC TTACAAGGAC 
TTCTACCGCG TTGTTGTGAG CGAAATGGGC AGCGGCGAGT GGACCAAGAT CCTCTACCAG 
TTGAAGCGCG AGTTTAACTA CTTTCGAAGG GATATTAGCA CCTTCCATCA GAAGGCTATG 
CACTCCTTTT CTTCTTCAGA TGACCCAGCC GACCAGCAAG CAGCCTTGAA CAGGATAACG 
GCTGAGGCGT CACGAGCCAT CTATGGCAAT TTCCTAGAGG CGATCAACAT GGCAACCTCG 
CTGTTTGCGC ACGATTACAT AGATCGTGAT TTGATGCGAA CCGGCATTTC TGTTGTCGTC 
ATAAGCCCTA GTCCGGGGAT TTTCGAAGTC GAGTACGACG CACTGCGCCG TACCACCGAA 
GCCTTGGTTG GAAATGGGAT TGGTATTGAT CTTATATGCA TACCCAAGGC TCCTCTCCAC 
TCCGTGCCCC TGTTTCGGTA TCGCAACCCT GAGTACTCGA CAGAACCGAC ACATCATCAC 
AAGGCAAAAA CAGCGCCGAG TCTTTCCAGT ACTCCTAAAC AAACAGCGCC GACGATTGGG 
AGCTTTAACA GCATGGCGGG GTCATATTCC CCAAGCAGAG GCACCGACAT CACTCTTCAT 
GGTGATTATG CTGGCTCTGG CTTGGCTCAA GATGAATGGA ATTATGCTTT GCCGCAATGG 
CTACATGTTT CTTACTGGAC AGGCGAGTCC GAGGAAGCCC TGTCATACCA TGGCATTGCT 
TTGTCGGTCT CTGATGTTTC AAAAACACGG TCAGGGGACG ACTTCTTGAT AAGATGTCGC 
ATGTATGACT TGCAAATGCG CAGCGTCATG GAAACCAATG AGATTGAAAC CAAGCCGTTG 
CATCTGGATC CTTATTTTCC TCATAAGGCA TTACAGGCAA ACAACGTACC GAAACCGTTT 
ATCGATCAAG ATGGCAACGT CTTCATACAA AACTTGCGCA TCCCAGAAAC ACTCTTTGAT 
CACGTGTACG GTTTCCAGAA ATTCGCCCCG GATAAGCACG CAAAGTTGGG GGAGAAGTCA 
CTCTGGAAGC AATTGCAGGA CTACGACGAC TCGAGAGCGC GCCAGCCGTC ATTTCGCCAC 
ACATTGGCGC CTCCGAGTCA TATTCGGGAG TACGATGATT CCGTCAGGCG ACATCATGCT 
GATGACGCGA GTCTTCTCGG GACGTCATAC ACTGAGCGTA GGCCGTCTAC GAGTGCCTCA 
CAAATGACTA TGACTGGGCA TAGATCCAGT CAGCGCTTCA ATGCCGACAA ACCTGAATCG 
CGTAACCGTA AGACGGTCGA CTTCGACAAA CTCAACTCCA AGCGTTCACC AACAATCACT 
CCAAAAGTTC CAAAGTTCAT GAAGCAGATC AGCCTTGGCA GCTCCGGTTT TCGCATTGCC 
GCACCCAAAG CTGCGACGGC AGAACTTAGC ATTGAAAGTG TGAATGCTGA TAGATCATTG 
GCATCTTCAA GGGCTTTGAT GAACGCGGGC ACTGTTGCAA CTCCAGCCAA AGCAGCAATC 
AACCGGTCAA TGTCGCCCCA GATGTTCGCG AGTGGAACTC CCGTTTTCTC CCCTTGGGGC 
GCTACACCAG GCTCATTCGC GCCCAGCCAT CAGTACTTTG AGGGTACTCC AAGCCGGCCG 
ATGCCAATCA AGTCGCAACA GATTCCTACT GACCCTGCAG CCAACATGCT ATCAGGCTCG 
ATGCTTGCCT CAACACTACG GGCCGATTAC GTTCCTGATA ATCGCGATCT CTTCTATTCA 
GATGCAATCA GAGCTGAGGA TGCGAAGAAG GTTTATAATA ACAAGCTTTT GGCCGGAGCG 
GTACCGGAAT TACCGTCAAC TTTATCGCCG ACAACCGCGC TGTCACCTTG GCTGACGTTG 
GTCAACCCGT CGAACCCTGA TAGCAACAAG GTCGATATTG CCACGTTGTA CAGTCGTTGG 
CAACATGTGT TTCCTAGGCC AAACGAGACG CGGGTGATGA AATGGAAGTC TTTGTGCTCC 
CCCGCTGCCG TTCCACTAAC CACAGAGTAC TTCCCTACCA AGGCACAGTT CGAGTCAGAG 
TACCACCGTC AACCGTATAA TGTCTCGCAA AATGCTGATG ATGAGCTGGC CGAGGAACCC 
AAGTCACGCG ACGAGATGCT GCGCGAGATG ATCAGTCTCA GATTCTGCCA AGGATTCCAG 
GTCGTTGTAG GCCCAGCTGT TGCCAAAGCC TTTGGCCAGA AGCAACTCAA GGTTACCGAC 
ATATTCTCCC GCGACCACAC AGCAGAGGAT GGTACAAGCA TCTTCATGTC GGTCGGCAAC 
ACGATCCACC AACTGTCGTG TGTGAATGGC ACCGAAATTG AAGTTAATAT CTTTGTCCGG 
AAGCCGATCG CCTCGGCGGG ACTGTACAAT GACCGTTACT TGTACAAACC GGCGATACGA 
ACTCTACTTG ACCACGGTTA CAAAACCCGT TCTTATGACA TGGTGGCCCC AACGCCTGAT 
CGGAACTGGA ATTACGTCGA TGCCTTTGTT GCAGGGCATA ATGACGAGTT GTCCGAACAT 
TTGAGGTTTT GGCGCGCTAG GTTCGTCCTG ATACCAATGA CAGCCAGACA CTCGTTTTTC 
CCCAAGACGC AGTACGGGGA CAGTGAAGAA GAGATTCGCA TCGAAGGCAT TCGGAAGCTT 
GCGCAGATGT GGCAGAAGCA CAGGTATATC CCGGCGAGCG AACGGAGCTT TCAAGCATCG 
CGTCGCAAAA AGGACATGAA TCCCCTTGAT ATCGTTTACA AAACAGAAGA TCCATCCGTA 
GTTATTGCGG CCGAGCTGGA AACGCTTCCG TTGTTGGAGG GCCTGGAAGG CGGGCACAGG 
AAGGGTCAGC TGGTCACGAG CAGAGAGCAA TTTTCGAAGA AGAACTTCAG CCTGGCAGCA 
TTGGCAGAGG CTATCCAGCA GCCCGTGGAA AATGGAGGCA TTCGAATGCA AAACCGGCGC 
TGGCATTTGC GCCTCCATTA CAACTGCTTC ATCGGCTCTG ACATGACCAC TTGGCTCCTG 
GAGAACTTCG AAGACTTGGA GGACAGAGAG GAAGCCGAAG AGCTTGGTAA CCGTCTCATG 
GTCACTTCAG ATGATAAGTC GAAGGATGAA AGCAAAGATG GGAGGAAGGA TGGCGGTGGA 
CTCTTCGTGC ACGTTGAGAG GCGACATCCT TTCAGAGATG GCCAGTACTT CTACCAGATC 
AGCAGCGACT ATGCGAAACC CAACCCTCCA GGCTGGTTTA ACACAAGGCG GGTTCCAGTG 
TCTGTCCCAT CAACACCGCA TTCGGAAGTA CGGGATTCAC CAAGGTCCAA TGTGTCGATG 
TCAAGACCAA CATCTATCCA CGAAGACAGC TCTCCCACCT CTGGAACGGT TACCCCAACG 
GCAGCTATGA TGGCGGGTGG CAAGAAGCCC AGGGTGGTTC TGAGTAAGGT CATGAAATAC 
GATGTTGACC ATCGAAAACG CTCTTACCGG CCCGAGGTGG TCGATTTGCA TTACGACAGA 
CTTCACAACC CCGACAATTG TTACCATATC CGGATCGACT GGATGAACGT AACAGCCAAG 
TTGATCGAAG ATGCTATTGA GGCATGGGCT CGCGAGGCAG CCTTGTACGG GCTCCGGCTC 
GTTGAGGTTC CTATCAACGA AGCTTGTGAT ATAACGGAAA TTAACCCGTT CCGCCGACCG 
TACATGATCA AGCTGGCTGT CCAGCCACCA AACCAACAGC CCATCACCTA CTTTGATCCC 
AATTCGTTTA CTCCGCAAGC ACAGCCAGGG CGGCACTTCT ATCAGAGGGC TCTCCTGAGA 
AAGTTTGACT TTGTTCTCGA CATGGAGCCA GCGTCCAGCT TCCCCTATAC CGTCGACGTG 
TCGTACTCAT GGGGCAAGCC GGACTTTAAA TACACGCAGT TCATCCACCG CTCCGGTACT 
CTCATTGCTG AAATCACCGA CGAAGGCGAC ATCCTGATCT TAGCCAACCG TCTCTATAGC 
AACCGCACGG CGGCCCAGCG TGAGAAGGAA ATGCAGCAGA AGGGAGGCGG ATCCGGTGCC 
GGGGGCGACA ACCTCGGACT CGGTCCCGGT CCAGGCCGCA TCTCAACGCC TGGTCTCTAC 
GGACCAAACG CTCACCCAGA CCAGCCAAAC GCAGTCTCCT CCCCGCTCGT CAAACCCACA 
ACAGCCTTCC TCTCCCCCGC GCTTCGGCCG CACCTCATCG GGCCCCTGGC CAGCGGCCCC 
CCATCAACCA ACGGATTCGG CACCACAGGC CAACCCCTCA ACCGATCCAC CGTCACCGTC 
CCCGTAACGC CCGTCATCCA AGACCCTGAG GTTATCAAAG ACGAGCTCGA ATCCTTCTGC 
CGCGACCGCT CCGCCCTCGA GGCCTTTTAC CGCGAATTGC TCGAGCGCGA AGCGCACCCG 
CCCGCACCCA ACACCACGCC CGGCCTTGCG CCCGTGAAGA TCCCGTCGAC CACAGCGACG 
AAGGACTCAA CCCTCGCCTT CGCTACTCCT CACCTCGGCG CCGGTCAAAA TACTACCAGC 
ACGGGTCACA CGGTTCCCGA TACGAACATC CCCAGCCTCG GCCTTCCTCC GGGCGTGCTA 
GCAGCAATAG GCGGCACCGG CCTGAGCAAT CTTAGTGACT TGCAGCTGGT AGGTAGCGGG 
ACTGGGCTGG GAATCAGTCC TGTACGAGTG GGAAGTCCGA GCTTGTTGTC GGCTGCGTCG 
GCTGCGTCTG CGGGCCACCC CTCGCACTCG ACGATGATGA GGAGAGCGAG CGTGCAGGAA 
GGGGTTATGG GTAGTAGGAT TGGTTTGGGC TTTGGGGGAG GTGCGTCAGC TGCAGCTGCA 
GCTGCTCAAG CGGCGGCGGC AGCGGCAGCG GCAGGAGCTT CGGGAGCGGG AGCTGCTGGT 
AGTAGTTCTG ATGCTGGTGG TGCCGGAAAC ATTGGTGGTG GTGCTGGCGG AGATGCAGGT 
GCTAGTGAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_962296.3
CDS	247..6261
Translation	MASRNFSSGVPGHVRPPNWSSHLRQFSRNSLERIPDMPRSPDTVSSNSTIRDRPSSSSRRSRFERRWTVTVNESFARDEVLLNLDLIGDEATPGSLMAIDVFRPDAEKQAQGSHHHHHHHHHHHHKQPPPDRTKEAAESQQRYIFVVKDMPKELKARYPSVEVYVAKHIADVFGMRKGSQVTLTPIDHHNPAIEASHVELCFRDQYMSRSDMWQLAVRELSEKTVYKGQMVLFMGTLKAQVTAVYVEGRKVPSAFFGHNTKPIFRSESARYVLFIQMAREMWDFDSDGSGEIMFNKVVNGFLPALFKKWASLKVRHLVSIVLFARVEYDTGISTELGNPDVQNDYYTGVQSSGDRRPYKDFYRVVVSEMGSGEWTKILYQLKREFNYFRRDISTFHQKAMHSFSSSDDPADQQAALNRITAEASRAIYGNFLEAINMATSLFAHDYIDRDLMRTGISVVVISPSPGIFEVEYDALRRTTEALVGNGIGIDLICIPKAPLHSVPLFRYRNPEYSTEPTHHHKAKTAPSLSSTPKQTAPTIGSFNSMAGSYSPSRGTDITLHGDYAGSGLAQDEWNYALPQWLHVSYWTGESEEALSYHGIALSVSDVSKTRSGDDFLIRCRMYDLQMRSVMETNEIETKPLHLDPYFPHKALQANNVPKPFIDQDGNVFIQNLRIPETLFDHVYGFQKFAPDKHAKLGEKSLWKQLQDYDDSRARQPSFRHTLAPPSHIREYDDSVRRHHADDASLLGTSYTERRPSTSASQMTMTGHRSSQRFNADKPESRNRKTVDFDKLNSKRSPTITPKVPKFMKQISLGSSGFRIAAPKAATAELSIESVNADRSLASSRALMNAGTVATPAKAAINRSMSPQMFASGTPVFSPWGATPGSFAPSHQYFEGTPSRPMPIKSQQIPTDPAANMLSGSMLASTLRADYVPDNRDLFYSDAIRAEDAKKVYNNKLLAGAVPELPSTLSPTTALSPWLTLVNPSNPDSNKVDIATLYSRWQHVFPRPNETRVMKWKSLCSPAAVPLTTEYFPTKAQFESEYHRQPYNVSQNADDELAEEPKSRDEMLREMISLRFCQGFQVVVGPAVAKAFGQKQLKVTDIFSRDHTAEDGTSIFMSVGNTIHQLSCVNGTEIEVNIFVRKPIASAGLYNDRYLYKPAIRTLLDHGYKTRSYDMVAPTPDRNWNYVDAFVAGHNDELSEHLRFWRARFVLIPMTARHSFFPKTQYGDSEEEIRIEGIRKLAQMWQKHRYIPASERSFQASRRKKDMNPLDIVYKTEDPSVVIAAELETLPLLEGLEGGHRKGQLVTSREQFSKKNFSLAALAEAIQQPVENGGIRMQNRRWHLRLHYNCFIGSDMTTWLLENFEDLEDREEAEELGNRLMVTSDDKSKDESKDGRKDGGGLFVHVERRHPFRDGQYFYQISSDYAKPNPPGWFNTRRVPVSVPSTPHSEVRDSPRSNVSMSRPTSIHEDSSPTSGTVTPTAAMMAGGKKPRVVLSKVMKYDVDHRKRSYRPEVVDLHYDRLHNPDNCYHIRIDWMNVTAKLIEDAIEAWAREAALYGLRLVEVPINEACDITEINPFRRPYMIKLAVQPPNQQPITYFDPNSFTPQAQPGRHFYQRALLRKFDFVLDMEPASSFPYTVDVSYSWGKPDFKYTQFIHRSGTLIAEITDEGDILILANRLYSNRTAAQREKEMQQKGGGSGAGGDNLGLGPGPGRISTPGLYGPNAHPDQPNAVSSPLVKPTTAFLSPALRPHLIGPLASGPPSTNGFGTTGQPLNRSTVTVPVTPVIQDPEVIKDELESFCRDRSALEAFYRELLEREAHPPAPNTTPGLAPVKIPSTTATKDSTLAFATPHLGAGQNTTSTGHTVPDTNIPSLGLPPGVLAAIGGTGLSNLSDLQLVGSGTGLGISPVRVGSPSLLSAASAASAGHPSHSTMMRRASVQEGVMGSRIGLGFGGGASAAAAAAQAAAAAAAAGASGAGAAGSSSDAGGAGNIGGGAGGDAGASEH

Target ORF information:

RefSeq Version	XM_957203.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A vacuolar membrane-associated protein iml-1 (NCU07710), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_957203.3

ORF Insert Sequence:

ATGGCGTCGC GCAATTTCTC TTCGGGGGTG CCGGGACATG TCAGGCCCCC CAACTGGTCG 
TCGCACCTCC GGCAGTTCAG CAGAAACAGC CTGGAGCGCA TCCCGGATAT GCCCCGGTCC 
CCGGACACTG TCTCTAGTAA CTCGACCATC CGTGACCGGC CCTCTTCATC CTCAAGACGT 
TCACGTTTCG AGAGGAGGTG GACCGTTACC GTCAACGAGT CCTTTGCTCG CGATGAAGTC 
TTGCTTAATC TTGATCTCAT AGGTGATGAG GCAACCCCAG GCAGCCTCAT GGCTATCGAC 
GTCTTCCGAC CTGATGCGGA GAAGCAAGCG CAAGGTTCTC ATCACCATCA CCACCACCAT 
CATCATCACC ATCATAAACA GCCTCCACCG GACCGCACAA AAGAGGCTGC AGAAAGTCAA 
CAGCGTTACA TCTTTGTCGT CAAGGACATG CCCAAGGAAC TCAAAGCCCG CTACCCGAGT 
GTTGAAGTCT ACGTCGCAAA GCACATTGCT GATGTCTTCG GCATGAGGAA GGGTTCGCAG 
GTTACCCTGA CCCCAATTGA TCATCATAAC CCAGCCATCG AGGCCTCGCA TGTCGAGCTT 
TGCTTTAGAG ACCAGTACAT GTCGCGCTCC GACATGTGGC AGTTGGCTGT TCGTGAGCTT 
TCAGAGAAGA CTGTCTACAA GGGCCAAATG GTTCTGTTCA TGGGCACTCT CAAGGCTCAG 
GTTACCGCTG TCTATGTTGA GGGCCGTAAG GTGCCATCCG CATTCTTTGG CCACAATACA 
AAACCCATCT TCCGAAGCGA GTCTGCTCGA TACGTGCTTT TCATCCAAAT GGCGCGCGAA 
ATGTGGGACT TTGATTCAGA TGGCTCTGGT GAGATCATGT TCAACAAGGT TGTGAACGGT 
TTTTTGCCAG CTCTGTTCAA GAAATGGGCG TCACTTAAAG TCAGGCACCT TGTCAGTATT 
GTTCTCTTTG CTCGAGTTGA GTATGACACA GGCATATCCA CCGAGTTGGG CAACCCGGAC 
GTCCAAAATG ACTACTACAC TGGTGTCCAG TCGTCTGGCG ATCGTCGCCC TTACAAGGAC 
TTCTACCGCG TTGTTGTGAG CGAAATGGGC AGCGGCGAGT GGACCAAGAT CCTCTACCAG 
TTGAAGCGCG AGTTTAACTA CTTTCGAAGG GATATTAGCA CCTTCCATCA GAAGGCTATG 
CACTCCTTTT CTTCTTCAGA TGACCCAGCC GACCAGCAAG CAGCCTTGAA CAGGATAACG 
GCTGAGGCGT CACGAGCCAT CTATGGCAAT TTCCTAGAGG CGATCAACAT GGCAACCTCG 
CTGTTTGCGC ACGATTACAT AGATCGTGAT TTGATGCGAA CCGGCATTTC TGTTGTCGTC 
ATAAGCCCTA GTCCGGGGAT TTTCGAAGTC GAGTACGACG CACTGCGCCG TACCACCGAA 
GCCTTGGTTG GAAATGGGAT TGGTATTGAT CTTATATGCA TACCCAAGGC TCCTCTCCAC 
TCCGTGCCCC TGTTTCGGTA TCGCAACCCT GAGTACTCGA CAGAACCGAC ACATCATCAC 
AAGGCAAAAA CAGCGCCGAG TCTTTCCAGT ACTCCTAAAC AAACAGCGCC GACGATTGGG 
AGCTTTAACA GCATGGCGGG GTCATATTCC CCAAGCAGAG GCACCGACAT CACTCTTCAT 
GGTGATTATG CTGGCTCTGG CTTGGCTCAA GATGAATGGA ATTATGCTTT GCCGCAATGG 
CTACATGTTT CTTACTGGAC AGGCGAGTCC GAGGAAGCCC TGTCATACCA TGGCATTGCT 
TTGTCGGTCT CTGATGTTTC AAAAACACGG TCAGGGGACG ACTTCTTGAT AAGATGTCGC 
ATGTATGACT TGCAAATGCG CAGCGTCATG GAAACCAATG AGATTGAAAC CAAGCCGTTG 
CATCTGGATC CTTATTTTCC TCATAAGGCA TTACAGGCAA ACAACGTACC GAAACCGTTT 
ATCGATCAAG ATGGCAACGT CTTCATACAA AACTTGCGCA TCCCAGAAAC ACTCTTTGAT 
CACGTGTACG GTTTCCAGAA ATTCGCCCCG GATAAGCACG CAAAGTTGGG GGAGAAGTCA 
CTCTGGAAGC AATTGCAGGA CTACGACGAC TCGAGAGCGC GCCAGCCGTC ATTTCGCCAC 
ACATTGGCGC CTCCGAGTCA TATTCGGGAG TACGATGATT CCGTCAGGCG ACATCATGCT 
GATGACGCGA GTCTTCTCGG GACGTCATAC ACTGAGCGTA GGCCGTCTAC GAGTGCCTCA 
CAAATGACTA TGACTGGGCA TAGATCCAGT CAGCGCTTCA ATGCCGACAA ACCTGAATCG 
CGTAACCGTA AGACGGTCGA CTTCGACAAA CTCAACTCCA AGCGTTCACC AACAATCACT 
CCAAAAGTTC CAAAGTTCAT GAAGCAGATC AGCCTTGGCA GCTCCGGTTT TCGCATTGCC 
GCACCCAAAG CTGCGACGGC AGAACTTAGC ATTGAAAGTG TGAATGCTGA TAGATCATTG 
GCATCTTCAA GGGCTTTGAT GAACGCGGGC ACTGTTGCAA CTCCAGCCAA AGCAGCAATC 
AACCGGTCAA TGTCGCCCCA GATGTTCGCG AGTGGAACTC CCGTTTTCTC CCCTTGGGGC 
GCTACACCAG GCTCATTCGC GCCCAGCCAT CAGTACTTTG AGGGTACTCC AAGCCGGCCG 
ATGCCAATCA AGTCGCAACA GATTCCTACT GACCCTGCAG CCAACATGCT ATCAGGCTCG 
ATGCTTGCCT CAACACTACG GGCCGATTAC GTTCCTGATA ATCGCGATCT CTTCTATTCA 
GATGCAATCA GAGCTGAGGA TGCGAAGAAG GTTTATAATA ACAAGCTTTT GGCCGGAGCG 
GTACCGGAAT TACCGTCAAC TTTATCGCCG ACAACCGCGC TGTCACCTTG GCTGACGTTG 
GTCAACCCGT CGAACCCTGA TAGCAACAAG GTCGATATTG CCACGTTGTA CAGTCGTTGG 
CAACATGTGT TTCCTAGGCC AAACGAGACG CGGGTGATGA AATGGAAGTC TTTGTGCTCC 
CCCGCTGCCG TTCCACTAAC CACAGAGTAC TTCCCTACCA AGGCACAGTT CGAGTCAGAG 
TACCACCGTC AACCGTATAA TGTCTCGCAA AATGCTGATG ATGAGCTGGC CGAGGAACCC 
AAGTCACGCG ACGAGATGCT GCGCGAGATG ATCAGTCTCA GATTCTGCCA AGGATTCCAG 
GTCGTTGTAG GCCCAGCTGT TGCCAAAGCC TTTGGCCAGA AGCAACTCAA GGTTACCGAC 
ATATTCTCCC GCGACCACAC AGCAGAGGAT GGTACAAGCA TCTTCATGTC GGTCGGCAAC 
ACGATCCACC AACTGTCGTG TGTGAATGGC ACCGAAATTG AAGTTAATAT CTTTGTCCGG 
AAGCCGATCG CCTCGGCGGG ACTGTACAAT GACCGTTACT TGTACAAACC GGCGATACGA 
ACTCTACTTG ACCACGGTTA CAAAACCCGT TCTTATGACA TGGTGGCCCC AACGCCTGAT 
CGGAACTGGA ATTACGTCGA TGCCTTTGTT GCAGGGCATA ATGACGAGTT GTCCGAACAT 
TTGAGGTTTT GGCGCGCTAG GTTCGTCCTG ATACCAATGA CAGCCAGACA CTCGTTTTTC 
CCCAAGACGC AGTACGGGGA CAGTGAAGAA GAGATTCGCA TCGAAGGCAT TCGGAAGCTT 
GCGCAGATGT GGCAGAAGCA CAGGTATATC CCGGCGAGCG AACGGAGCTT TCAAGCATCG 
CGTCGCAAAA AGGACATGAA TCCCCTTGAT ATCGTTTACA AAACAGAAGA TCCATCCGTA 
GTTATTGCGG CCGAGCTGGA AACGCTTCCG TTGTTGGAGG GCCTGGAAGG CGGGCACAGG 
AAGGGTCAGC TGGTCACGAG CAGAGAGCAA TTTTCGAAGA AGAACTTCAG CCTGGCAGCA 
TTGGCAGAGG CTATCCAGCA GCCCGTGGAA AATGGAGGCA TTCGAATGCA AAACCGGCGC 
TGGCATTTGC GCCTCCATTA CAACTGCTTC ATCGGCTCTG ACATGACCAC TTGGCTCCTG 
GAGAACTTCG AAGACTTGGA GGACAGAGAG GAAGCCGAAG AGCTTGGTAA CCGTCTCATG 
GTCACTTCAG ATGATAAGTC GAAGGATGAA AGCAAAGATG GGAGGAAGGA TGGCGGTGGA 
CTCTTCGTGC ACGTTGAGAG GCGACATCCT TTCAGAGATG GCCAGTACTT CTACCAGATC 
AGCAGCGACT ATGCGAAACC CAACCCTCCA GGCTGGTTTA ACACAAGGCG GGTTCCAGTG 
TCTGTCCCAT CAACACCGCA TTCGGAAGTA CGGGATTCAC CAAGGTCCAA TGTGTCGATG 
TCAAGACCAA CATCTATCCA CGAAGACAGC TCTCCCACCT CTGGAACGGT TACCCCAACG 
GCAGCTATGA TGGCGGGTGG CAAGAAGCCC AGGGTGGTTC TGAGTAAGGT CATGAAATAC 
GATGTTGACC ATCGAAAACG CTCTTACCGG CCCGAGGTGG TCGATTTGCA TTACGACAGA 
CTTCACAACC CCGACAATTG TTACCATATC CGGATCGACT GGATGAACGT AACAGCCAAG 
TTGATCGAAG ATGCTATTGA GGCATGGGCT CGCGAGGCAG CCTTGTACGG GCTCCGGCTC 
GTTGAGGTTC CTATCAACGA AGCTTGTGAT ATAACGGAAA TTAACCCGTT CCGCCGACCG 
TACATGATCA AGCTGGCTGT CCAGCCACCA AACCAACAGC CCATCACCTA CTTTGATCCC 
AATTCGTTTA CTCCGCAAGC ACAGCCAGGG CGGCACTTCT ATCAGAGGGC TCTCCTGAGA 
AAGTTTGACT TTGTTCTCGA CATGGAGCCA GCGTCCAGCT TCCCCTATAC CGTCGACGTG 
TCGTACTCAT GGGGCAAGCC GGACTTTAAA TACACGCAGT TCATCCACCG CTCCGGTACT 
CTCATTGCTG AAATCACCGA CGAAGGCGAC ATCCTGATCT TAGCCAACCG TCTCTATAGC 
AACCGCACGG CGGCCCAGCG TGAGAAGGAA ATGCAGCAGA AGGGAGGCGG ATCCGGTGCC 
GGGGGCGACA ACCTCGGACT CGGTCCCGGT CCAGGCCGCA TCTCAACGCC TGGTCTCTAC 
GGACCAAACG CTCACCCAGA CCAGCCAAAC GCAGTCTCCT CCCCGCTCGT CAAACCCACA 
ACAGCCTTCC TCTCCCCCGC GCTTCGGCCG CACCTCATCG GGCCCCTGGC CAGCGGCCCC 
CCATCAACCA ACGGATTCGG CACCACAGGC CAACCCCTCA ACCGATCCAC CGTCACCGTC 
CCCGTAACGC CCGTCATCCA AGACCCTGAG GTTATCAAAG ACGAGCTCGA ATCCTTCTGC 
CGCGACCGCT CCGCCCTCGA GGCCTTTTAC CGCGAATTGC TCGAGCGCGA AGCGCACCCG 
CCCGCACCCA ACACCACGCC CGGCCTTGCG CCCGTGAAGA TCCCGTCGAC CACAGCGACG 
AAGGACTCAA CCCTCGCCTT CGCTACTCCT CACCTCGGCG CCGGTCAAAA TACTACCAGC 
ACGGGTCACA CGGTTCCCGA TACGAACATC CCCAGCCTCG GCCTTCCTCC GGGCGTGCTA 
GCAGCAATAG GCGGCACCGG CCTGAGCAAT CTTAGTGACT TGCAGCTGGT AGGTAGCGGG 
ACTGGGCTGG GAATCAGTCC TGTACGAGTG GGAAGTCCGA GCTTGTTGTC GGCTGCGTCG 
GCTGCGTCTG CGGGCCACCC CTCGCACTCG ACGATGATGA GGAGAGCGAG CGTGCAGGAA 
GGGGTTATGG GTAGTAGGAT TGGTTTGGGC TTTGGGGGAG GTGCGTCAGC TGCAGCTGCA 
GCTGCTCAAG CGGCGGCGGC AGCGGCAGCG GCAGGAGCTT CGGGAGCGGG AGCTGCTGGT 
AGTAGTTCTG ATGCTGGTGG TGCCGGAAAC ATTGGTGGTG GTGCTGGCGG AGATGCAGGT 
GCTAGTGAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU07710 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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