NCU00066 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00066
Entrez Gene ID	3872537
Full Name	pre-mRNA-splicing factor cwc-22
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951283.3	XP_956376.3	pre-mRNA-splicing factor cwc-22

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00066 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00066 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10036	XM_951283.3 Latest version!	Neurospora crassa OR74A pre-mRNA-splicing factor cwc-22 (NCU00066), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00066 ( XM_951283.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956376.3 Neurospora crassa OR74A pre-mRNA-splicing factor cwc-22 (NCU00066), mRNA.

Price & Availability↑

CloneID	ONf10036
Clone ID Related Accession (Same CDS sequence)	XM_951283.3
Accession Version	XM_951283.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	pre-mRNA-splicing factor cwc-22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_951283.2.

ATGGCTTCGG CAGACATGTC TCCGTCGCGT AGCCATCCTC ACGACGCGAC CCGATCCCCG 
TCCCCCAGGA CCCAGTCACC TTCGCCCCGG GACGAAGACG GCTCTCGGTC CCCTGGTGAA 
CGTACACCCT CACCTCCTTC TCGCGATCCG TCGCCGTATC GATCACCAGG CGAACGCACA 
CCTTCGCCAT CTCCTCGCCG GGATCGCTCT CTCTCACCAA GAGATCAGCC GCACTCACAC 
CCACGATCCC GTTCGCCTAC ACCCCGCAGC CAATCGCCTT CCCGACGCTC TGTCCGCTCG 
CCATCTCCGA GACAGGGCTC ACCAGCGCGC CGTGTTGATC GCTCATCTTC CCCTCGGGCA 
CGCTCGCCGC CACCACGCCG TCATTCCCGT TCTCCTCCGC TGAGAGGACA ACCCCCTCCT 
CCGCGCCATC GCGATGCAGG TGGCGACTAT AGACCGGTAA GGAAGGAGCG CACCCCGACG 
CCACCCCCTG TGGCTGTCAA GACCGAGGAG GAAAAGCTTG CCGACGCCAG AGCAGAGTAC 
CAGAAGCTCT TGAACCTACG CAGTCAGGGT GTGTACCTAC CCCCGCACAG GCTACGTGCC 
CTCCAGGCTG CCATCACCGA CAAGAAGACC AGGGAATACC AGCGTATGGC TTGGGAGGCA 
CTGAAGAAGT CTGTCAACGG TCTGGTCAAC AAGGTCAACA CTGCCAACAT CAAGTTTGTG 
GTCCCCGAAC TCTTTGGCGA GAACCTTATC CGTGGCCGCG GTCTGTTTTG CCAGAGTCTG 
CTGAAGGCCC AACATGCCAG TTTGCCCTTC ACCCCCATTT ATGCTTGCTT GGCTGCGATT 
TGCAACACCA AGTTACCCCA GGTGGGCGAA CTCCTTGTCA AGAGGCTCGT CCTGCGTTTC 
CGCAAGGCGT TCAAGAGAAA CGACAAGGCG GTTTGCCTCT CGTCGACCAT GTTCATAGCC 
CATCTCGTCA ACAACCAGGT TGTCCACGAG ATGATTGCCG CCCAGATTCT GCTGCTGCTG 
CTTGCCAAGC CCACGGATGA CAGCGTCGAA ATCGCTGTCG GCCTCATGAG GGAAGTCGGC 
TTGTTTTTGG AGGAGATGTC CCCGGCCATC GCCCATGCCG TTTTCGACCA GTTCCGCAAC 
ATTCTTCACG AGGCCGATAT CGACCGTCGT ACGCAGTACA TGATCGAGGT GCTTTTCCAG 
GTGCGCAAGG ACAAGTACAA GGACAACCCG GTTATCAAGG AGGAATTGGA TCTCGTCGAA 
GAGGAGGACC AAATCACACA CCGGATTGGG CTTGACGACG AAATTGACCC GCAAGATGGA 
CTCAATGTGT TCAAGATGGA TCCCAACTGG GAGGAGAACG AGGAGGAATA CAAGAAGTTG 
AAGGCGGAGA TCTTGGGTGA GGCTAGCGAC GACGACGAAG ATGACGATGA CGACGACGAG 
TCGGAGAGCG GATCGGAATC GGAGGACGAG GAACAGAAGG CGTTGGAGAT CAAGGACCAG 
AGTAATGCGG ACCTCGTCAA CCTCAGGAGG ACTATCTATC TCAGCATCCA GTCGAGTGCC 
GATCCCGAAG AAGCGGCGCA CAAGCTTATG AAGCTGCGGC TCCCTGCCGG CCAGGAGGCC 
GAGTTGGTGT CGATGATTGT CGAGTCATGC GCCCAGGAGA AGGTCTACTT GAAGTTTATG 
GGCTTGCTAG GAGAGCGGTT TGCCCGTCTT AACAGGATGT GGATGGACTT GTTCGAGGAG 
TCATTTGCCA AGTACTACTC CACGATTCAC CGATACGAGA CCAACAAGCT TCGCAACATT 
GCTAGGTTCT TTGGGCATCT TTTGGCTACC GATGCCATCG GCTGGCACGT TTTCTCGGTT 
ATCCACCTAA ACGAGGAAGA AACGACCTCC GCCAGCCGTA TCTTTATCAA GATCTTGTTT 
GAAGATCTCC AGGAGAATAT TGGCTCTGCG AAGTTGAAGG CTCGCATGAG TGAAGAGACC 
CTCCAGCCCA GTCTGCAAGG CATCTTCCCT CATGATGAGC CGCGTAACAT TCGCTTCTCT 
ATCAACTACT TCACATCCAT CAAGATGGGC TATCTTACTG ACGAGATGCG TACCTTCCTA 
GCAAACATGC CAAAACCTGC CCTGCCAGCA CCTCCAGCGG ATTCCGATTC CGAGTCCGTG 
TCCAGCTACT CTTCCTATTC TTCTTACTCA TCCCGTTCTC GCTCTCGTTC CCTGACACCT 
CGGAAGGACA CTCGCGGACG TTCGCTCTCG CGCACCCCTC CTCGTCGTGG CAGAGGCAGA 
TCCTACAGCC GGACACCAAG CCGGAGCAGG AGTCGTAGCC GTAGCTACAG CCGCTCCGTC 
TCGAAATCCG TTTCGCGGTC TCCTCCAAGG CGAAGAGCGG TGGAGTCCCG GTCTCCCAGC 
CCGCCACCCC GCGGCCGTGG GCGTAGCTAC GACCGGTACT CCCGCTCACC CTCGCGCTCG 
CGCTCTCGTA CCCGCTCCCC GGCGGCGGCG CCGCCCATCC GCCGCGGTCG CTCAGGAACC 
CGTAGCCGTA GCAGATCATA TTCGCGCTCG CCATCCCCCC CGCCTGCTCG TGGTTATCCC 
ACCCGTGGTC GAGCACCCGT TAGCAACAAC GACAGAGCCG CCGCCGCTTC CGGAAAGCGT 
CGTAGGGAAG GTTCCTACTC GGCAAGTCGC TCGCCACATC CCCCTCCCCA ACAGCGTCTT 
CGCCGAGGGA GCTACAGTCG GTCACGATCA AGGTCTCCGA TACCGATCAG AGGCAACGGG 
CCGGCGGGTA GGGATACAGG CCGAGCTGGC CCGGCTCCCG CTCGTGGAGG ACGGCGGAAT 
CGCTCATACT CGAGATCGCG AACCCGGTCA CCTCCACCAC TAGCGGATGC CGCCACGGGG 
TCTAGGAGAG TTGTCAGCAG GTCGCCAAGT CCGGTGGTTG GCAACAACAA GCGGAGGAGA 
TCGTACAGCA GCTCTAGGTC AAGATCTAGA TCGAGTTCGA GGTCCAGGTA CAGGTCCAGG 
TCACCAGTTG CTAAGCGGGG AAGAGTTGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956376.3
CDS	265..3297
Translation	MASADMSPSRSHPHDATRSPSPRTQSPSPRDEDGSRSPGERTPSPPSRDPSPYRSPGERTPSPSPRRDRSLSPRDQPHSHPRSRSPTPRSQSPSRRSVRSPSPRQGSPARRVDRSSSPRARSPPPRRHSRSPPLRGQPPPPRHRDAGGDYRPVRKERTPTPPPVAVKTEEEKLADARAEYQKLLNLRSQGVYLPPHRLRALQAAITDKKTREYQRMAWEALKKSVNGLVNKVNTANIKFVVPELFGENLIRGRGLFCQSLLKAQHASLPFTPIYACLAAICNTKLPQVGELLVKRLVLRFRKAFKRNDKAVCLSSTMFIAHLVNNQVVHEMIAAQILLLLLAKPTDDSVEIAVGLMREVGLFLEEMSPAIAHAVFDQFRNILHEADIDRRTQYMIEVLFQVRKDKYKDNPVIKEELDLVEEEDQITHRIGLDDEIDPQDGLNVFKMDPNWEENEEEYKKLKAEILGEASDDDEDDDDDDESESGSESEDEEQKALEIKDQSNADLVNLRRTIYLSIQSSADPEEAAHKLMKLRLPAGQEAELVSMIVESCAQEKVYLKFMGLLGERFARLNRMWMDLFEESFAKYYSTIHRYETNKLRNIARFFGHLLATDAIGWHVFSVIHLNEEETTSASRIFIKILFEDLQENIGSAKLKARMSEETLQPSLQGIFPHDEPRNIRFSINYFTSIKMGYLTDEMRTFLANMPKPALPAPPADSDSESVSSYSSYSSYSSRSRSRSLTPRKDTRGRSLSRTPPRRGRGRSYSRTPSRSRSRSRSYSRSVSKSVSRSPPRRRAVESRSPSPPPRGRGRSYDRYSRSPSRSRSRTRSPAAAPPIRRGRSGTRSRSRSYSRSPSPPPARGYPTRGRAPVSNNDRAAAASGKRRREGSYSASRSPHPPPQQRLRRGSYSRSRSRSPIPIRGNGPAGRDTGRAGPAPARGGRRNRSYSRSRTRSPPPLADAATGSRRVVSRSPSPVVGNNKRRRSYSSSRSRSRSSSRSRYRSRSPVAKRGRVD

Target ORF information:

RefSeq Version	XM_951283.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A pre-mRNA-splicing factor cwc-22 (NCU00066), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951283.3

ORF Insert Sequence:

ATGGCTTCGG CAGACATGTC TCCGTCGCGT AGCCATCCTC ACGACGCGAC CCGATCCCCG 
TCCCCCAGGA CCCAGTCACC TTCGCCCCGG GACGAAGACG GCTCTCGGTC CCCTGGTGAA 
CGTACACCCT CACCTCCTTC TCGCGATCCG TCGCCGTATC GATCACCAGG CGAACGCACA 
CCTTCGCCAT CTCCTCGCCG GGATCGCTCT CTCTCACCAA GAGATCAGCC GCACTCACAC 
CCACGATCCC GTTCGCCTAC ACCCCGCAGC CAATCGCCTT CCCGACGCTC TGTCCGCTCG 
CCATCTCCGA GACAGGGCTC ACCAGCGCGC CGTGTTGATC GCTCATCTTC CCCTCGGGCA 
CGCTCGCCGC CACCACGCCG TCATTCCCGT TCTCCTCCGC TGAGAGGACA ACCCCCTCCT 
CCGCGCCATC GCGATGCAGG TGGCGACTAT AGACCGGTAA GGAAGGAGCG CACCCCGACG 
CCACCCCCTG TGGCTGTCAA GACCGAGGAG GAAAAGCTTG CCGACGCCAG AGCAGAGTAC 
CAGAAGCTCT TGAACCTACG CAGTCAGGGT GTGTACCTAC CCCCGCACAG GCTACGTGCC 
CTCCAGGCTG CCATCACCGA CAAGAAGACC AGGGAATACC AGCGTATGGC TTGGGAGGCA 
CTGAAGAAGT CTGTCAACGG TCTGGTCAAC AAGGTCAACA CTGCCAACAT CAAGTTTGTG 
GTCCCCGAAC TCTTTGGCGA GAACCTTATC CGTGGCCGCG GTCTGTTTTG CCAGAGTCTG 
CTGAAGGCCC AACATGCCAG TTTGCCCTTC ACCCCCATTT ATGCTTGCTT GGCTGCGATT 
TGCAACACCA AGTTACCCCA GGTGGGCGAA CTCCTTGTCA AGAGGCTCGT CCTGCGTTTC 
CGCAAGGCGT TCAAGAGAAA CGACAAGGCG GTTTGCCTCT CGTCGACCAT GTTCATAGCC 
CATCTCGTCA ACAACCAGGT TGTCCACGAG ATGATTGCCG CCCAGATTCT GCTGCTGCTG 
CTTGCCAAGC CCACGGATGA CAGCGTCGAA ATCGCTGTCG GCCTCATGAG GGAAGTCGGC 
TTGTTTTTGG AGGAGATGTC CCCGGCCATC GCCCATGCCG TTTTCGACCA GTTCCGCAAC 
ATTCTTCACG AGGCCGATAT CGACCGTCGT ACGCAGTACA TGATCGAGGT GCTTTTCCAG 
GTGCGCAAGG ACAAGTACAA GGACAACCCG GTTATCAAGG AGGAATTGGA TCTCGTCGAA 
GAGGAGGACC AAATCACACA CCGGATTGGG CTTGACGACG AAATTGACCC GCAAGATGGA 
CTCAATGTGT TCAAGATGGA TCCCAACTGG GAGGAGAACG AGGAGGAATA CAAGAAGTTG 
AAGGCGGAGA TCTTGGGTGA GGCTAGCGAC GACGACGAAG ATGACGATGA CGACGACGAG 
TCGGAGAGCG GATCGGAATC GGAGGACGAG GAACAGAAGG CGTTGGAGAT CAAGGACCAG 
AGTAATGCGG ACCTCGTCAA CCTCAGGAGG ACTATCTATC TCAGCATCCA GTCGAGTGCC 
GATCCCGAAG AAGCGGCGCA CAAGCTTATG AAGCTGCGGC TCCCTGCCGG CCAGGAGGCC 
GAGTTGGTGT CGATGATTGT CGAGTCATGC GCCCAGGAGA AGGTCTACTT GAAGTTTATG 
GGCTTGCTAG GAGAGCGGTT TGCCCGTCTT AACAGGATGT GGATGGACTT GTTCGAGGAG 
TCATTTGCCA AGTACTACTC CACGATTCAC CGATACGAGA CCAACAAGCT TCGCAACATT 
GCTAGGTTCT TTGGGCATCT TTTGGCTACC GATGCCATCG GCTGGCACGT TTTCTCGGTT 
ATCCACCTAA ACGAGGAAGA AACGACCTCC GCCAGCCGTA TCTTTATCAA GATCTTGTTT 
GAAGATCTCC AGGAGAATAT TGGCTCTGCG AAGTTGAAGG CTCGCATGAG TGAAGAGACC 
CTCCAGCCCA GTCTGCAAGG CATCTTCCCT CATGATGAGC CGCGTAACAT TCGCTTCTCT 
ATCAACTACT TCACATCCAT CAAGATGGGC TATCTTACTG ACGAGATGCG TACCTTCCTA 
GCAAACATGC CAAAACCTGC CCTGCCAGCA CCTCCAGCGG ATTCCGATTC CGAGTCCGTG 
TCCAGCTACT CTTCCTATTC TTCTTACTCA TCCCGTTCTC GCTCTCGTTC CCTGACACCT 
CGGAAGGACA CTCGCGGACG TTCGCTCTCG CGCACCCCTC CTCGTCGTGG CAGAGGCAGA 
TCCTACAGCC GGACACCAAG CCGGAGCAGG AGTCGTAGCC GTAGCTACAG CCGCTCCGTC 
TCGAAATCCG TTTCGCGGTC TCCTCCAAGG CGAAGAGCGG TGGAGTCCCG GTCTCCCAGC 
CCGCCACCCC GCGGCCGTGG GCGTAGCTAC GACCGGTACT CCCGCTCACC CTCGCGCTCG 
CGCTCTCGTA CCCGCTCCCC GGCGGCGGCG CCGCCCATCC GCCGCGGTCG CTCAGGAACC 
CGTAGCCGTA GCAGATCATA TTCGCGCTCG CCATCCCCCC CGCCTGCTCG TGGTTATCCC 
ACCCGTGGTC GAGCACCCGT TAGCAACAAC GACAGAGCCG CCGCCGCTTC CGGAAAGCGT 
CGTAGGGAAG GTTCCTACTC GGCAAGTCGC TCGCCACATC CCCCTCCCCA ACAGCGTCTT 
CGCCGAGGGA GCTACAGTCG GTCACGATCA AGGTCTCCGA TACCGATCAG AGGCAACGGG 
CCGGCGGGTA GGGATACAGG CCGAGCTGGC CCGGCTCCCG CTCGTGGAGG ACGGCGGAAT 
CGCTCATACT CGAGATCGCG AACCCGGTCA CCTCCACCAC TAGCGGATGC CGCCACGGGG 
TCTAGGAGAG TTGTCAGCAG GTCGCCAAGT CCGGTGGTTG GCAACAACAA GCGGAGGAGA 
TCGTACAGCA GCTCTAGGTC AAGATCTAGA TCGAGTTCGA GGTCCAGGTA CAGGTCCAGG 
TCACCAGTTG CTAAGCGGGG AAGAGTTGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU00066 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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