NCU03575 cDNA ORF clone, Neurospora crassa OR74A

The following NCU03575 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03575 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf05135 XM_950921.2
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Neurospora crassa OR74A isoleucyl-tRNA synthetase (NCU03575), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf05135
    Clone ID Related Accession (Same CDS sequence) XM_950921.2
    Accession Version XM_950921.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product isoleucyl-tRNA synthetase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502). On Feb 23, 2015 this sequence version replaced XM_950921.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCGATCG ATTTCCCCAA GGAAGAGGAA AACACCATCC AGCGATGGCG CGAGATCAAG 
    GCCTTTGAGA CCCAGGTCGA GCTGTCCAAG GGCAACCCGC TCTACACCTT CTACGATGGC
    CCGCCCTTCG CCACCGGCCT CCCCCACTAC GGCCACTTGC TGGCCTCGAC CATCAAGGAC
    ATCATCCCCC GATACTGGTC CATGAAGGGC TTCCACGTCG AGCGCCGCTT CGGCTGGGAC
    ACGCACGGCC TGCCCATCGA GCACGAGATC GACAAGAAGC TGGGCATCTC GGGCAAGGCC
    GCCGTCATGG AGATTGGCCT CGAGAACTAC AACGCCGAGT GCCGGTCCAT CGTCATGAGG
    TATCGCGAGG AGTGGCGCCA CACGGTCGAG CGTCTCGGTC GCTGGATCGA CTTTGACAAC
    GACTACAAGA CCATGGACCC GACCTTCATG GAGTCCGAGT GGTGGGTCTT CAAGCAGCTG
    TTCGACAAGG GCCAGGTCTA CCGCGGCTAC CGCGTCATGC CCTACTCGAC CGCCCTCACC
    ACCGCCCTCT CCAACTTCGA GGCCAACCAG AACTACCAGG ACGTCACCGA TCCCGCCGTC
    GTCGTCGCCT TCCCCTTGAT CGAGGACCCC AACACCTGCC TCCTTGCCTG GACCACCACG
    CCCTGGACCC TGCCCTCCCA CACCGGCCTG GCCGCCCACC CCGACTTTGA ATATGTCAAG
    ATCGCCGACG AGAAGAGCGG AAAGAACTAC ATCCTGCTCG AGAAGCTCTT GACGACGCTC
    TACAAGGACC CCAAGAAGGC CAAGTTCAAG GTGCTCGAGA AGATCAAGGG CAAGGACATG
    CTCGGCTGGA AGTACGAGCC CCCCTTCAAC TACTTCTACG ACGAGTACAA GGACGTTGCC
    TTCAAGGTCC TCAACGCTAC CTACGTCACT GACGACAGCG GTACCGGTAT TGTCCACCAG
    GCCCCCGCCT TCGGTGAGGA CGATTACAAC GTGGCCGTCG AGGCGGGTAT CGTCACCGAG
    AAGCGCCCCC CTCCCGACCC GGTCAACGAG ACGGGTCACT ACACCGACCG CGTCTCCGAT
    TTCGCCGGCA TGCACGTCAA GGAGGCCGAC AAGCACATCA TCAAGCACCT CAAGAACTCG
    GGCAAGCTCG TTGTCGAGTC TCAGCTCAAG CACTCGTACC CCATGTGCTA CCGCTCCGAC
    ACGCCCCTCA TCTACAAGGC CGTGCCCTCA TGGTTCATCC GCATCCCCGA CATCATCCCC
    CAGATGTTGA AGAACATTGA GGGCTCCCAC TGGGTCCCCT CGTTCGTCAA GGAGAAGCGT
    TTCGCCAGCT GGATCGCCAA CGCCCGTGAC TGGAACGTCT CACGTAACAG GTACTGGGGT
    ACTCCCATCC CTCTGTGGAT GAGCGACGAC GGCGAGGAGA TTGTGTGCAT TGGCAGCATC
    GAGGAGCTCA AGCAGCTTTC CGGCTACGAG GGCGAGATCA CCGATCTCCA CCGTGACAAG
    ATCGACCACA TCACCATCCC CAGCAAGCAG GGCAAGGGTG TCCTCAAGCG TGCTGAGGAG
    GTCTTTGACT GCTGGTTCGA GTCTGGTTCC ATGCCCTACG CCTCCCAGCA CTACCCCTTT
    GAGAACGTCG AGAAGTTCGA AAAGTCATTC CCCGGTGACT TCATTGCCGA GGGTCTTGAC
    CAGACCCGTG GTTGGTTCTA CACCCTTCTT GTCTTGGGTA CCCACCTCTT TGGTGTCTCT
    CCTTTCAAGA ACTGCGTCGT CAACGGTATC GTGCTTGCCG AGGATGGCAA GAAGATGTCC
    AAGCGTCTCA AGAACTATCC CGACCCTACT CTTGTCATGA ACAAGTATGG TTCGGATGCC
    CTCCGTCTGT ACCTTATCAA CTCTCCTGTT GTGCGTGCCG AGCCGCTCCG CTTCAAGGAG
    GCTGGTGTCA AGGAGGTCGT TGCCAAGGTT CTCCTTCCTC TGTGGAACTC GTACAAGTTC
    TTCGAGGGCC AGGTTGCTCT CCTCAAGAAG GTGGAGAACG TCGACTATGT GTTTGACCCC
    AAGATGGAGT CTTCCAACGA GAACGTGATG GACAAGTGGA TTCTTGCTTC GTGCCAGTCC
    CTGCTCGAGT TTGTCAACGA GGAGATGAAG GGTTACCGCC TCTACACTGT TGTCCCTCGC
    CTCCTCGAGC TCATCGACAA CACCACCAAC TGGTACATCC GCTTCAACCG CAAGCGTCTC
    AAGGGTGAGA ACGGTCTCAA CGACACCCTC CACGCCCTCA ACACGCTCTT CGAGGTCCTC
    TTCACCCTCT GCCGTGGCCT CGCCCCCTTC ACTCCCTTCC TTACCGACAC CATCTACCTC
    AAGCTCCTTC CCCACATTCC CAAGGAGCTC CAGGCCAAGG ACCCCCGCTC CGTGCACTTC
    CTCCCCTTCC CCGAGGTTCG CAAGGAGCTC TACAACCCCG ACGTCGAGCG CCGCGTGCAG
    CGCATGCAGA AGGTCATCGA GCTCGCCCGA TACTCGCGCG AGAAGCGTTC GGTCGGTCTC
    AAGCAGCCCC TCAAGACCCT TGTTGTCATC CACCACGACG AGCAGTACCT TGCCGACATC
    GAGTCGCTCA AGGGCTACAT TACCGAGGAG CTCAACGTGC GCGACCTGGT GCTCACTTCA
    GACGAAGCCA AGCACGGCGT TCAGTACTCG GTCTCGGCCG ACTGGCCTGT GCTCGGCAAG
    AAGCTCAAGA AGGACATGGG CCGCGTCAAG AAGGCTCTTC CCTCGGTCAC CTCGGATGAG
    GCCCACGCCT ACACGCAGAC GGGCAAGCTC GTTGTGGACG GCATCACGCT CGAGGCGGGC
    GACCTGGTGG TCAAGCGCGG TCTCAAGGAG GACGACAGCT CCAAGAACCT CGAGACCAAC
    ACGGACAACG ACGTGCTCAC CATCTTGGAC GTGGAGATTT ACCCCGGCCT GGCGGAGGAG
    GGTCTGGCGC GTGAAATCAT CAACCGTGTG CAGAGGCTGC GCAAGAAGGC GGGTCTCCAG
    CCGACGGATG ATATCAAGAT GGAGTACAAG GTGACGGCGG ACCCCGAGGA GACGGGTATT
    GAGAAGGTGT TTGACACGCA GATGGCCATG ATTGAGAAGG CGCTCAGGAG GCCGCTGGAT
    AAGCACGAGG TGACGCATGT GGAGGGCAAG ATTCCGGCGG AGAAGGAGGA GGGCATCATT
    ATGGAGGAGG AGCAGGAGGT TGGCAAGGCT ACCTTCTTGT TGAGGTTGTT GAGGCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_956014.1
    CDS143..3382
    Translation

    Target ORF information:

    RefSeq Version XM_950921.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A isoleucyl-tRNA synthetase (NCU03575), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_950921.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCGATCG ATTTCCCCAA GGAAGAGGAA AACACCATCC AGCGATGGCG CGAGATCAAG 
    GCCTTTGAGA CCCAGGTCGA GCTGTCCAAG GGCAACCCGC TCTACACCTT CTACGATGGC
    CCGCCCTTCG CCACCGGCCT CCCCCACTAC GGCCACTTGC TGGCCTCGAC CATCAAGGAC
    ATCATCCCCC GATACTGGTC CATGAAGGGC TTCCACGTCG AGCGCCGCTT CGGCTGGGAC
    ACGCACGGCC TGCCCATCGA GCACGAGATC GACAAGAAGC TGGGCATCTC GGGCAAGGCC
    GCCGTCATGG AGATTGGCCT CGAGAACTAC AACGCCGAGT GCCGGTCCAT CGTCATGAGG
    TATCGCGAGG AGTGGCGCCA CACGGTCGAG CGTCTCGGTC GCTGGATCGA CTTTGACAAC
    GACTACAAGA CCATGGACCC GACCTTCATG GAGTCCGAGT GGTGGGTCTT CAAGCAGCTG
    TTCGACAAGG GCCAGGTCTA CCGCGGCTAC CGCGTCATGC CCTACTCGAC CGCCCTCACC
    ACCGCCCTCT CCAACTTCGA GGCCAACCAG AACTACCAGG ACGTCACCGA TCCCGCCGTC
    GTCGTCGCCT TCCCCTTGAT CGAGGACCCC AACACCTGCC TCCTTGCCTG GACCACCACG
    CCCTGGACCC TGCCCTCCCA CACCGGCCTG GCCGCCCACC CCGACTTTGA ATATGTCAAG
    ATCGCCGACG AGAAGAGCGG AAAGAACTAC ATCCTGCTCG AGAAGCTCTT GACGACGCTC
    TACAAGGACC CCAAGAAGGC CAAGTTCAAG GTGCTCGAGA AGATCAAGGG CAAGGACATG
    CTCGGCTGGA AGTACGAGCC CCCCTTCAAC TACTTCTACG ACGAGTACAA GGACGTTGCC
    TTCAAGGTCC TCAACGCTAC CTACGTCACT GACGACAGCG GTACCGGTAT TGTCCACCAG
    GCCCCCGCCT TCGGTGAGGA CGATTACAAC GTGGCCGTCG AGGCGGGTAT CGTCACCGAG
    AAGCGCCCCC CTCCCGACCC GGTCAACGAG ACGGGTCACT ACACCGACCG CGTCTCCGAT
    TTCGCCGGCA TGCACGTCAA GGAGGCCGAC AAGCACATCA TCAAGCACCT CAAGAACTCG
    GGCAAGCTCG TTGTCGAGTC TCAGCTCAAG CACTCGTACC CCATGTGCTA CCGCTCCGAC
    ACGCCCCTCA TCTACAAGGC CGTGCCCTCA TGGTTCATCC GCATCCCCGA CATCATCCCC
    CAGATGTTGA AGAACATTGA GGGCTCCCAC TGGGTCCCCT CGTTCGTCAA GGAGAAGCGT
    TTCGCCAGCT GGATCGCCAA CGCCCGTGAC TGGAACGTCT CACGTAACAG GTACTGGGGT
    ACTCCCATCC CTCTGTGGAT GAGCGACGAC GGCGAGGAGA TTGTGTGCAT TGGCAGCATC
    GAGGAGCTCA AGCAGCTTTC CGGCTACGAG GGCGAGATCA CCGATCTCCA CCGTGACAAG
    ATCGACCACA TCACCATCCC CAGCAAGCAG GGCAAGGGTG TCCTCAAGCG TGCTGAGGAG
    GTCTTTGACT GCTGGTTCGA GTCTGGTTCC ATGCCCTACG CCTCCCAGCA CTACCCCTTT
    GAGAACGTCG AGAAGTTCGA AAAGTCATTC CCCGGTGACT TCATTGCCGA GGGTCTTGAC
    CAGACCCGTG GTTGGTTCTA CACCCTTCTT GTCTTGGGTA CCCACCTCTT TGGTGTCTCT
    CCTTTCAAGA ACTGCGTCGT CAACGGTATC GTGCTTGCCG AGGATGGCAA GAAGATGTCC
    AAGCGTCTCA AGAACTATCC CGACCCTACT CTTGTCATGA ACAAGTATGG TTCGGATGCC
    CTCCGTCTGT ACCTTATCAA CTCTCCTGTT GTGCGTGCCG AGCCGCTCCG CTTCAAGGAG
    GCTGGTGTCA AGGAGGTCGT TGCCAAGGTT CTCCTTCCTC TGTGGAACTC GTACAAGTTC
    TTCGAGGGCC AGGTTGCTCT CCTCAAGAAG GTGGAGAACG TCGACTATGT GTTTGACCCC
    AAGATGGAGT CTTCCAACGA GAACGTGATG GACAAGTGGA TTCTTGCTTC GTGCCAGTCC
    CTGCTCGAGT TTGTCAACGA GGAGATGAAG GGTTACCGCC TCTACACTGT TGTCCCTCGC
    CTCCTCGAGC TCATCGACAA CACCACCAAC TGGTACATCC GCTTCAACCG CAAGCGTCTC
    AAGGGTGAGA ACGGTCTCAA CGACACCCTC CACGCCCTCA ACACGCTCTT CGAGGTCCTC
    TTCACCCTCT GCCGTGGCCT CGCCCCCTTC ACTCCCTTCC TTACCGACAC CATCTACCTC
    AAGCTCCTTC CCCACATTCC CAAGGAGCTC CAGGCCAAGG ACCCCCGCTC CGTGCACTTC
    CTCCCCTTCC CCGAGGTTCG CAAGGAGCTC TACAACCCCG ACGTCGAGCG CCGCGTGCAG
    CGCATGCAGA AGGTCATCGA GCTCGCCCGA TACTCGCGCG AGAAGCGTTC GGTCGGTCTC
    AAGCAGCCCC TCAAGACCCT TGTTGTCATC CACCACGACG AGCAGTACCT TGCCGACATC
    GAGTCGCTCA AGGGCTACAT TACCGAGGAG CTCAACGTGC GCGACCTGGT GCTCACTTCA
    GACGAAGCCA AGCACGGCGT TCAGTACTCG GTCTCGGCCG ACTGGCCTGT GCTCGGCAAG
    AAGCTCAAGA AGGACATGGG CCGCGTCAAG AAGGCTCTTC CCTCGGTCAC CTCGGATGAG
    GCCCACGCCT ACACGCAGAC GGGCAAGCTC GTTGTGGACG GCATCACGCT CGAGGCGGGC
    GACCTGGTGG TCAAGCGCGG TCTCAAGGAG GACGACAGCT CCAAGAACCT CGAGACCAAC
    ACGGACAACG ACGTGCTCAC CATCTTGGAC GTGGAGATTT ACCCCGGCCT GGCGGAGGAG
    GGTCTGGCGC GTGAAATCAT CAACCGTGTG CAGAGGCTGC GCAAGAAGGC GGGTCTCCAG
    CCGACGGATG ATATCAAGAT GGAGTACAAG GTGACGGCGG ACCCCGAGGA GACGGGTATT
    GAGAAGGTGT TTGACACGCA GATGGCCATG ATTGAGAAGG CGCTCAGGAG GCCGCTGGAT
    AAGCACGAGG TGACGCATGT GGAGGGCAAG ATTCCGGCGG AGAAGGAGGA GGGCATCATT
    ATGGAGGAGG AGCAGGAGGT TGGCAAGGCT ACCTTCTTGT TGAGGTTGTT GAGGCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B