COL8A1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	COL8A1
Entrez Gene ID	107109963
Full Name	collagen type VIII alpha 1 chain
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015410675.1	XP_015266161.1	LOW QUALITY PROTEIN: collagen alpha-1(VIII) chain

Pathways from BioSystems
Gene Ontology

The following COL8A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL8A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe16078	XM_015410675.1 Latest version!	Gekko japonicus collagen, type VIII, alpha 1 (COL8A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL8A1 ( XM_015410675.1 ) cDNA ORF clone, Gekko japonicus -> XP_015266161.1 Gekko japonicus collagen, type VIII, alpha 1 (COL8A1), mRNA.

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CloneID	OGe16078
Clone ID Related Accession (Same CDS sequence)	XM_015410675.1
Accession Version	XM_015410675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	LOW QUALITY PROTEIN: collagen alpha-1(VIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015160216.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTGTGC TGCTAGCACC AACTTGGCTA TTGGTGGTGG TTGTAACCAT TTACATTGGG 
CTACTGAGAT CAACTCAAGC TGGTGCTTAT TATGGGATCA AGCAGATGCC TCCCCAAGTC 
CCACAGTACC AAGCCCTTGG CCAACAAGGA CAACAAGTAC CTCACATGCC AATGGGCAAA 
GATGGCATTC CACTGCAGCG CATGGGCAAG GAGGGGCCAC CACCCATGCC ATACGGGAAA 
GAATATATCC CTCAGTTTTT GAAAGAAATC CCACCACTAC CTATGCTTGG CAAGGAGAGG 
ATTTCAAAGA AACAGAAAGA AATCCCATCA ATTGGTTTGA GAGGAGAGCG TGGTCCTCCT 
GGGGAGCCTG GACCAAGAGG GCCCACTGGA CCTCCAGGGA TACCAGGACA TGGAGTGCCA 
GGAGCCAAAG GAAAANNCNN NNNNNNNNNN NNNNNNNNNN NNNTGCCTGG ACTGCCAGGA 
AAACCAGGAC CAATGGGGCC CCCTGGACCA AGAGGCGAGA TGGGGCCAAA GGGAGAGATT 
GGACCCATGG GTATTCCAGG ACCCCAAGGC CTACCAGGAC CCCATGGACT ACCTGGAATA 
GGAAAACCAG GTGATCGAGG TTTGCCAGGG CAACCAGGAC CAAAGGCTGA GCCAGGAATG 
AAAGGACAGC CTGGAATACA AGGTCCCACT GGTCCAAAAG GGGATAAAGG TGTTGGGATT 
CCAGGACTGC CTGGGTTAAA AGGTCCACCT GGTTTGCCTG GTCCTCCAGG TCCTGTTGGA 
CTTCCAGGAA TAGGCAAGCC AGGACTGACT GGATTTCCAG GCCCACAGGG ACCTGCAGGA 
AAACCTGGGC TTCCAGGGAA GCCAGGGCCT CAGGGGTTAA ATGGAGCCCC TGGAATTCAA 
GGTCCTCCTG GCCTTCCAGG TATAGGAAAG CCAGGGCAAG ATGGAATCCC AGGTCAACCA 
GGGTTTCCTG GTGGAAAAGG TGAGCAAGGT TTGCCAGGTT TACCAGGACC TCCTGGATTA 
CCTGGGATAG GGAAGCCAGG TTTCCCTGGA CCTAAAGGTG ACCGTGGCAT GGGTGGTGTT 
CCAGGGCCAC TTGGGCCTAA GGGTGAAAAG GGGCACATGG GGCCACCTGG CATGGGTGGG 
CCACCAGGAG AGCCGGGCCA ACCAGGGCTG CCAGGACTTA TGGGTCCTTC AGGTGCTATG 
GGGTTCCCAG GCCCTAAAGG AGAAGTTGGA GTTGTTGGGC CTCCAGGGCC ACTTGGTCCA 
AAAGGTGAAC TTGGCTTACA GGGTTTCCCA GGAAAACCTG GCTTCCCTGG GGAAGTGGGA 
CCTCCAGGTC TAAGAGGTTT ACCAGGCCCA ATGGGACCGA AAGGAGAAGT TGGTCACAAA 
GGCTTACCTG GTCTGCCTGG TGCTCATGGA CCTATGGGGC CCAAAGGAGA ACCTGGAATC 
CCAGGAGCTC AAGGACATCA AGGCCCTTCT GGAATCCCAG GCATAGCAGG ACCCAGTGGC 
CCAATTGGAC CTCCTGGACT TCCTGGACCT AAAGGGGAAC GAGGTGTGCA AGGTCCACCA 
GGCTTTCCAG GAATGGGGAA GCCTGGTATT GCAGGGGTAC AAGGGCCACC TGGCAAGCCT 
GGAATGATTG GTTTACCTGG CCAGCCAGGC TCCCAGGGAC CTCCAGGACC TCCTGGTCCC 
CCCGGCCCCC CAGTAATAGT CCAACCCACA CCACCAGCAA TTGGACAATA TTTGCCTGAG 
ATGGGTCCAG GCATTGATGG AGTAAAGACT CCACATGGCT ACATGGGGAA GAAAGGCAAA 
AATGGTGGTG TGGCCTATGA GATGCCAGCT TTCACAGCCG AGCTCACCAC TCCTTTCCCA 
CGAGTTGGGG TGCCTGTGAT GTTTGACAAG CTCCTCTATA ATGGCAGGCA GAATTATAAT 
CCACAGACTG GCATCTTCAC CTGTGAAATC CCTGGGATTT ACTACTTCGC CTACCACATT 
CACTGTAAAG GAGGGAATGT CTGGGTGGCT TTGTTCAAAA ACAATGATCC ATTGATGTAC 
ACCTACGATG AATACAAGAA AGGCTTCCTG GACCAAGCTT CAGGGAGTGC CGTCGTCCAG 
CTGATGCAGG GTGACAGAGT GTATATCCAG ATGCCATCCG AACAGGCAGC AGGACTCTAT 
GCCGGGCAAT ACGTCCATTC ATCTTTTTCA GGATATTTGT TGTATCCCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015266161.1
CDS	101..2314
Translation	MAVLLAPTWLLVVVVTIYIGLLRSTQAGAYYGIKQMPPQVPQYQALGQQGQQVPHMPMGKDGIPLQRMGKEGPPPMPYGKEYIPQFLKEIPPLPMLGKERISKKQKEIPSIGLRGERGPPGEPGPRGPTGPPGIPGHGVPGAKGKnullnullnullnullnullnullnullnullnullnullPGLPGKPGPMGPPGPRGEMGPKGEIGPMGIPGPQGLPGPHGLPGIGKPGDRGLPGQPGPKAEPGMKGQPGIQGPTGPKGDKGVGIPGLPGLKGPPGLPGPPGPVGLPGIGKPGLTGFPGPQGPAGKPGLPGKPGPQGLNGAPGIQGPPGLPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGIGKPGFPGPKGDRGMGGVPGPLGPKGEKGHMGPPGMGGPPGEPGQPGLPGLMGPSGAMGFPGPKGEVGVVGPPGPLGPKGELGLQGFPGKPGFPGEVGPPGLRGLPGPMGPKGEVGHKGLPGLPGAHGPMGPKGEPGIPGAQGHQGPSGIPGIAGPSGPIGPPGLPGPKGERGVQGPPGFPGMGKPGIAGVQGPPGKPGMIGLPGQPGSQGPPGPPGPPGPPVIVQPTPPAIGQYLPEMGPGIDGVKTPHGYMGKKGKNGGVAYEMPAFTAELTTPFPRVGVPVMFDKLLYNGRQNYNPQTGIFTCEIPGIYYFAYHIHCKGGNVWVALFKNNDPLMYTYDEYKKGFLDQASGSAVVQLMQGDRVYIQMPSEQAAGLYAGQYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	XM_015410675.1
Organism	Gekko japonicus
Definition	Gekko japonicus collagen, type VIII, alpha 1 (COL8A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015410675.1

ORF Insert Sequence:

ATGGCTGTGC TGCTAGCACC AACTTGGCTA TTGGTGGTGG TTGTAACCAT TTACATTGGG 
CTACTGAGAT CAACTCAAGC TGGTGCTTAT TATGGGATCA AGCAGATGCC TCCCCAAGTC 
CCACAGTACC AAGCCCTTGG CCAACAAGGA CAACAAGTAC CTCACATGCC AATGGGCAAA 
GATGGCATTC CACTGCAGCG CATGGGCAAG GAGGGGCCAC CACCCATGCC ATACGGGAAA 
GAATATATCC CTCAGTTTTT GAAAGAAATC CCACCACTAC CTATGCTTGG CAAGGAGAGG 
ATTTCAAAGA AACAGAAAGA AATCCCATCA ATTGGTTTGA GAGGAGAGCG TGGTCCTCCT 
GGGGAGCCTG GACCAAGAGG GCCCACTGGA CCTCCAGGGA TACCAGGACA TGGAGTGCCA 
GGAGCCAAAG GAAAANNCNN NNNNNNNNNN NNNNNNNNNN NNNTGCCTGG ACTGCCAGGA 
AAACCAGGAC CAATGGGGCC CCCTGGACCA AGAGGCGAGA TGGGGCCAAA GGGAGAGATT 
GGACCCATGG GTATTCCAGG ACCCCAAGGC CTACCAGGAC CCCATGGACT ACCTGGAATA 
GGAAAACCAG GTGATCGAGG TTTGCCAGGG CAACCAGGAC CAAAGGCTGA GCCAGGAATG 
AAAGGACAGC CTGGAATACA AGGTCCCACT GGTCCAAAAG GGGATAAAGG TGTTGGGATT 
CCAGGACTGC CTGGGTTAAA AGGTCCACCT GGTTTGCCTG GTCCTCCAGG TCCTGTTGGA 
CTTCCAGGAA TAGGCAAGCC AGGACTGACT GGATTTCCAG GCCCACAGGG ACCTGCAGGA 
AAACCTGGGC TTCCAGGGAA GCCAGGGCCT CAGGGGTTAA ATGGAGCCCC TGGAATTCAA 
GGTCCTCCTG GCCTTCCAGG TATAGGAAAG CCAGGGCAAG ATGGAATCCC AGGTCAACCA 
GGGTTTCCTG GTGGAAAAGG TGAGCAAGGT TTGCCAGGTT TACCAGGACC TCCTGGATTA 
CCTGGGATAG GGAAGCCAGG TTTCCCTGGA CCTAAAGGTG ACCGTGGCAT GGGTGGTGTT 
CCAGGGCCAC TTGGGCCTAA GGGTGAAAAG GGGCACATGG GGCCACCTGG CATGGGTGGG 
CCACCAGGAG AGCCGGGCCA ACCAGGGCTG CCAGGACTTA TGGGTCCTTC AGGTGCTATG 
GGGTTCCCAG GCCCTAAAGG AGAAGTTGGA GTTGTTGGGC CTCCAGGGCC ACTTGGTCCA 
AAAGGTGAAC TTGGCTTACA GGGTTTCCCA GGAAAACCTG GCTTCCCTGG GGAAGTGGGA 
CCTCCAGGTC TAAGAGGTTT ACCAGGCCCA ATGGGACCGA AAGGAGAAGT TGGTCACAAA 
GGCTTACCTG GTCTGCCTGG TGCTCATGGA CCTATGGGGC CCAAAGGAGA ACCTGGAATC 
CCAGGAGCTC AAGGACATCA AGGCCCTTCT GGAATCCCAG GCATAGCAGG ACCCAGTGGC 
CCAATTGGAC CTCCTGGACT TCCTGGACCT AAAGGGGAAC GAGGTGTGCA AGGTCCACCA 
GGCTTTCCAG GAATGGGGAA GCCTGGTATT GCAGGGGTAC AAGGGCCACC TGGCAAGCCT 
GGAATGATTG GTTTACCTGG CCAGCCAGGC TCCCAGGGAC CTCCAGGACC TCCTGGTCCC 
CCCGGCCCCC CAGTAATAGT CCAACCCACA CCACCAGCAA TTGGACAATA TTTGCCTGAG 
ATGGGTCCAG GCATTGATGG AGTAAAGACT CCACATGGCT ACATGGGGAA GAAAGGCAAA 
AATGGTGGTG TGGCCTATGA GATGCCAGCT TTCACAGCCG AGCTCACCAC TCCTTTCCCA 
CGAGTTGGGG TGCCTGTGAT GTTTGACAAG CTCCTCTATA ATGGCAGGCA GAATTATAAT 
CCACAGACTG GCATCTTCAC CTGTGAAATC CCTGGGATTT ACTACTTCGC CTACCACATT 
CACTGTAAAG GAGGGAATGT CTGGGTGGCT TTGTTCAAAA ACAATGATCC ATTGATGTAC 
ACCTACGATG AATACAAGAA AGGCTTCCTG GACCAAGCTT CAGGGAGTGC CGTCGTCCAG 
CTGATGCAGG GTGACAGAGT GTATATCCAG ATGCCATCCG AACAGGCAGC AGGACTCTAT 
GCCGGGCAAT ACGTCCATTC ATCTTTTTCA GGATATTTGT TGTATCCCAT GTAA