LOC102444127 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102444127
Entrez Gene ID	102444127
Full Name	toll-like receptor 9
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014571799.2	XP_014427285.2	toll-like receptor 9
XM_014571799.1	XP_014427285.1	toll-like receptor 9

Pathways from BioSystems
Gene Ontology

The following LOC102444127 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102444127 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe149614	XM_014571799.2 Latest version!	Pelodiscus sinensis toll-like receptor 9 (LOC102444127), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPe26198	XM_014571799.1 Latest version!	Pelodiscus sinensis toll-like receptor 9 (LOC102444127), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102444127 ( XM_014571799.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014427285.2 Pelodiscus sinensis toll-like receptor 9 (LOC102444127), mRNA.

Price & Availability↑

CloneID	OPe149614
Clone ID Related Accession (Same CDS sequence)	XM_014571799.2
Accession Version	XM_014571799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	toll-like receptor 9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854103.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_014571799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACCA GTGCTTCCGT CCGCTCAAAA ATTGAGTGCA GCTACCAGAA AACAAAGGTG 
AGCATGCTGC TGTGGCGGGT GTGTCTGCTG GCCCAGGGCC TCCTGGGGGC CGGCGCCCTC 
TTCCCTAGTT ACCCGCCCTG CGCTAACAGC AAAGACGGCA AGAACGTGGA CTGCAGCCTG 
CGCAGCCTCA CCCTGGTGCC AGACATACGT TCCAGCAGCG TGACCTCCCT GAACCTGGAC 
AGCAACTTCC TAGCCCTGCT GGCCAATGGC TCCTTCCAGG GCGTCCCTTG CCTGAGGGAT 
CTCTCGCTCA GCTGGAACTG CATGCCGGGG CAGCTGCGGA AAGCGCACGC GGTGTGTCAG 
CTGGTGATCC AGCCAGGGGC CCTGACCGTG CTGCGCCACC TCACCAACCT GAGCCTGGCA 
GGAAACAGCC TGAGCGTGCT GCCCCCGCTG CCCTGCACCC TCCGCTTCCT GGACTTGAGT 
TACAACAACA TCCTCAGGCT GGGCCCTCGG AGCCTGGTGG AGCTCAGCCA CGTGAGCGAG 
CTTCTCCTAG GCAACAACTG CTACTACCGG AACCCGTGCG GCTCCTCCGT CAGCCTCGCA 
CGAGATGCCT TCCTGCCCCT GACCTCCCTG AGGCGGCTCT CGCTGAAGTT TAACAACATG 
ACTGCAACTC CCCAGGGGCT CCCGCCCACG CTGCACCAGC TCGACCTCTC GGAGAACAAG 
ATCGCCCACG TGAGCCACCT GGAGAACCTC ACCAGCCTGA GGTCCCTCAA CCTGGAGTGG 
AACTGCCAGC GCTGTGACCA TGCTGCTCAG CCCTGCTTCC CCTGCCCAGG CAACAAGGCC 
CTGACCTTCG AGCAGGATGC TTTCCAGAAC CTCGCGCAGC TCAGGAGCTT GAACCTGCGA 
GGCAACTCCC TGCAGGACCT CAACGCCAGC TTCTTCAGCA GCCTCCGCAA CTTAGAGACC 
TTGGATCTGT CAGACAACTT CCTGACCTGC GCCATTAGAC ATGGCACCTT CTTCTCCGTG 
CTGAGCTCAG TGCAGACCCT AAACCTGGGC TACAATTACC AGTCACTGGT GACCTTCGAG 
AATCTGACTC TGTCTGACTC CTTCGGCCAA ATGACATCTC TGAAAAATCT CATCATCAAT 
GGCTACTTCT TCAGGCACTT GGCTGAGGAC GGGATCAAGC CTCTCTTCAA CCTGAGCAAC 
TTGGAGACCC TCCAATTTCG AACCAACTTC ATCAGGAACG TGAATCTGTC CCTCTTTTCC 
AAGTGCAGGA CTCTCACGTT CATCAGTCTG TCCCAGAACA ACATTGTCTT CTCCCCCCCA 
TGCGAGAGAG GTGGGAATGG AGCTGGAACG GGACCTCGGG GCCTCTCCCA TGATGGCACT 
TTGGTGGGGA ATCCCAGACG CTGGGCGGAA CCCAAAGGAA GAGCTGAGGC CATGGTGAGG 
GACAGCCAAA GCTCCCATGC CAACCTCCCG GCAGGCGTTC GCTGCAGCAA GACCCTGGAT 
TTATCATTCA ATAGCATCCA CAGCATTGAG CCAGCCCAGT TCCAAGGGCT AGAGGCAATT 
GACTGCCTCA ACCTCAGCTC TAATTACATC AGCCAGTCGC TAAATGGCAG CCAGTTCCTG 
CCGCTGCGGT CCCTGAAGCT GCTGGATCTG TCTCACAATA GGTTCGACCT GTACTATGAC 
AGCGCCCTGC AAGAGGTCTG CCAGCTGGTC GTGCTGAACC TCAGCAATAA TCAATTCCAC 
TTCACAATCA AAGGCCTGGG GCACAAGTTT GAGTTCTTGA AAAACCTCAC CAACTTGAAA 
TTTCTGAACC TGGACAACAA CCAGATCGGT ATCAGGATCT CCAAACACCT GGAAAGCGCT 
GGACATCATC TGCCCAGTCT AAGGCTCCTG GACATTTCGT TTAACAACTT GGACATCATG 
TGGATGAGCG GGAGGAATAC CTACCTGGAG TTCTTTGTCA AGCTGCCCAG TCTAAGGCTC 
CTGGACATTT CGTTTAACAA CTTGGACTCC ATTCCAGCTC AGGTCATTGA AACCTTGCCT 
GGAAGCTTGA GGAAATTGTC CATTGCCTGT AACAATCTCT ACGACTTCCC CTGGGACAAA 
ATGGAAAAGC TGAGCAGCTT GGAATTCCTA GACTTGAGTT ATAATGCACT GGAGACGCTG 
CTGGTCGTGG GCAGAGGCTC CTTTGGCAAG AACCTCACCA CCCTCGACCT CAGCTACAAC 
AAAATCAAGT CTCTCCAGGA AGCGTTCTTT TCCCACGCCA GTTCCTTGCA CTTTCTCAGT 
TTGAACCATA ACAAGATCCA GATGGTCGAT GAAAGCAGCT TTCCCAAAGC CCTTCTTCAG 
AGCTTGCAGC AGCTGGACGT GAGCAGCAAT CCCTTGCGGT GCACCTGTGC AGCCCACTGG 
TTCCTCACCT TCCTCAAAAG CACGTCCATC ACCATAGACC ATTTCTCCAC TAGCATGCAA 
TGTGACCTGC CCGAATCAAA GCGGGGGCAG CCTCTGCTCT CCATGGACCC TCGGTCCTGC 
CAGGATATAT ACGGTCACCT AAGCTTCCTG TGCTCTTCCC TCCTGGTGAC CTTGGTGACT 
GTGCTTCCAG TCCTCCATAA GTTGTATGGC TGGGACCTCT GGTATGTCAG TCACCTCTGC 
CTGGCCACAC TCAGACAAGG CTATGCCCAG GTGGCCACAG CCTCCCGGGA GGAATACGAT 
GCATTCGTAG CCTTTGACGC CCAACACAGC CTGGTGGCTG ACTGGGTCTA CAATGAGATG 
GTGCCCCAGC TGGAGGAGAA AGGAAGAAGG AGATTCAAGC TGTGCCTGGA GGAAAGGGAC 
TGGACAGTGG GCGGGTCCAG GATTGAGAAC CTCTGTGACT CCATCTACAA GAGCAGGAAA 
ACCATCTTCA TCCTGGGGAG AGAAGGGTTT GGCAGCGGCT TCCTGCGGCA CACCTTCCTC 
CTCAGCCAGC AGCGGCTGCT GGACGAGAAG GTAGATGTGC TCCTGCTGGT GCTACTGGAC 
CCCGCGATGA AGATGTCCAG GTTCCTGCTG GCCCGGAGAA GACTGTGTGG GAGGACAGTC 
CTGGACTGGC CCTGCAACCC CTTTGCCCAG CCCTACTTCT GGCACTGCGT GCGAGCCCGG 
CTGGCCTGCG AGGTTCATCC CTTTGATGCC TCACACCTGA GGAGAGGCGT CAGCAGTTCT 
GGGGCAGGGG AGCTCCTGCC AGCAGAGGAC GCCATGGCCA GGCCGGCTTT GCAAAGGCCC 
CGAGAAGCAG CCAGGGCTTG CGCATGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014427285.2
CDS	1330..4602
Translation	MRTSASVRSKIECSYQKTKVSMLLWRVCLLAQGLLGAGALFPSYPPCANSKDGKNVDCSLRSLTLVPDIRSSSVTSLNLDSNFLALLANGSFQGVPCLRDLSLSWNCMPGQLRKAHAVCQLVIQPGALTVLRHLTNLSLAGNSLSVLPPLPCTLRFLDLSYNNILRLGPRSLVELSHVSELLLGNNCYYRNPCGSSVSLARDAFLPLTSLRRLSLKFNNMTATPQGLPPTLHQLDLSENKIAHVSHLENLTSLRSLNLEWNCQRCDHAAQPCFPCPGNKALTFEQDAFQNLAQLRSLNLRGNSLQDLNASFFSSLRNLETLDLSDNFLTCAIRHGTFFSVLSSVQTLNLGYNYQSLVTFENLTLSDSFGQMTSLKNLIINGYFFRHLAEDGIKPLFNLSNLETLQFRTNFIRNVNLSLFSKCRTLTFISLSQNNIVFSPPCERGGNGAGTGPRGLSHDGTLVGNPRRWAEPKGRAEAMVRDSQSSHANLPAGVRCSKTLDLSFNSIHSIEPAQFQGLEAIDCLNLSSNYISQSLNGSQFLPLRSLKLLDLSHNRFDLYYDSALQEVCQLVVLNLSNNQFHFTIKGLGHKFEFLKNLTNLKFLNLDNNQIGIRISKHLESAGHHLPSLRLLDISFNNLDIMWMSGRNTYLEFFVKLPSLRLLDISFNNLDSIPAQVIETLPGSLRKLSIACNNLYDFPWDKMEKLSSLEFLDLSYNALETLLVVGRGSFGKNLTTLDLSYNKIKSLQEAFFSHASSLHFLSLNHNKIQMVDESSFPKALLQSLQQLDVSSNPLRCTCAAHWFLTFLKSTSITIDHFSTSMQCDLPESKRGQPLLSMDPRSCQDIYGHLSFLCSSLLVTLVTVLPVLHKLYGWDLWYVSHLCLATLRQGYAQVATASREEYDAFVAFDAQHSLVADWVYNEMVPQLEEKGRRRFKLCLEERDWTVGGSRIENLCDSIYKSRKTIFILGREGFGSGFLRHTFLLSQQRLLDEKVDVLLLVLLDPAMKMSRFLLARRRLCGRTVLDWPCNPFAQPYFWHCVRARLACEVHPFDASHLRRGVSSSGAGELLPAEDAMARPALQRPREAARACAWQ

Target ORF information:

RefSeq Version	XM_014571799.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis toll-like receptor 9 (LOC102444127), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014571799.2

ORF Insert Sequence:

ATGAGGACCA GTGCTTCCGT CCGCTCAAAA ATTGAGTGCA GCTACCAGAA AACAAAGGTG 
AGCATGCTGC TGTGGCGGGT GTGTCTGCTG GCCCAGGGCC TCCTGGGGGC CGGCGCCCTC 
TTCCCTAGTT ACCCGCCCTG CGCTAACAGC AAAGACGGCA AGAACGTGGA CTGCAGCCTG 
CGCAGCCTCA CCCTGGTGCC AGACATACGT TCCAGCAGCG TGACCTCCCT GAACCTGGAC 
AGCAACTTCC TAGCCCTGCT GGCCAATGGC TCCTTCCAGG GCGTCCCTTG CCTGAGGGAT 
CTCTCGCTCA GCTGGAACTG CATGCCGGGG CAGCTGCGGA AAGCGCACGC GGTGTGTCAG 
CTGGTGATCC AGCCAGGGGC CCTGACCGTG CTGCGCCACC TCACCAACCT GAGCCTGGCA 
GGAAACAGCC TGAGCGTGCT GCCCCCGCTG CCCTGCACCC TCCGCTTCCT GGACTTGAGT 
TACAACAACA TCCTCAGGCT GGGCCCTCGG AGCCTGGTGG AGCTCAGCCA CGTGAGCGAG 
CTTCTCCTAG GCAACAACTG CTACTACCGG AACCCGTGCG GCTCCTCCGT CAGCCTCGCA 
CGAGATGCCT TCCTGCCCCT GACCTCCCTG AGGCGGCTCT CGCTGAAGTT TAACAACATG 
ACTGCAACTC CCCAGGGGCT CCCGCCCACG CTGCACCAGC TCGACCTCTC GGAGAACAAG 
ATCGCCCACG TGAGCCACCT GGAGAACCTC ACCAGCCTGA GGTCCCTCAA CCTGGAGTGG 
AACTGCCAGC GCTGTGACCA TGCTGCTCAG CCCTGCTTCC CCTGCCCAGG CAACAAGGCC 
CTGACCTTCG AGCAGGATGC TTTCCAGAAC CTCGCGCAGC TCAGGAGCTT GAACCTGCGA 
GGCAACTCCC TGCAGGACCT CAACGCCAGC TTCTTCAGCA GCCTCCGCAA CTTAGAGACC 
TTGGATCTGT CAGACAACTT CCTGACCTGC GCCATTAGAC ATGGCACCTT CTTCTCCGTG 
CTGAGCTCAG TGCAGACCCT AAACCTGGGC TACAATTACC AGTCACTGGT GACCTTCGAG 
AATCTGACTC TGTCTGACTC CTTCGGCCAA ATGACATCTC TGAAAAATCT CATCATCAAT 
GGCTACTTCT TCAGGCACTT GGCTGAGGAC GGGATCAAGC CTCTCTTCAA CCTGAGCAAC 
TTGGAGACCC TCCAATTTCG AACCAACTTC ATCAGGAACG TGAATCTGTC CCTCTTTTCC 
AAGTGCAGGA CTCTCACGTT CATCAGTCTG TCCCAGAACA ACATTGTCTT CTCCCCCCCA 
TGCGAGAGAG GTGGGAATGG AGCTGGAACG GGACCTCGGG GCCTCTCCCA TGATGGCACT 
TTGGTGGGGA ATCCCAGACG CTGGGCGGAA CCCAAAGGAA GAGCTGAGGC CATGGTGAGG 
GACAGCCAAA GCTCCCATGC CAACCTCCCG GCAGGCGTTC GCTGCAGCAA GACCCTGGAT 
TTATCATTCA ATAGCATCCA CAGCATTGAG CCAGCCCAGT TCCAAGGGCT AGAGGCAATT 
GACTGCCTCA ACCTCAGCTC TAATTACATC AGCCAGTCGC TAAATGGCAG CCAGTTCCTG 
CCGCTGCGGT CCCTGAAGCT GCTGGATCTG TCTCACAATA GGTTCGACCT GTACTATGAC 
AGCGCCCTGC AAGAGGTCTG CCAGCTGGTC GTGCTGAACC TCAGCAATAA TCAATTCCAC 
TTCACAATCA AAGGCCTGGG GCACAAGTTT GAGTTCTTGA AAAACCTCAC CAACTTGAAA 
TTTCTGAACC TGGACAACAA CCAGATCGGT ATCAGGATCT CCAAACACCT GGAAAGCGCT 
GGACATCATC TGCCCAGTCT AAGGCTCCTG GACATTTCGT TTAACAACTT GGACATCATG 
TGGATGAGCG GGAGGAATAC CTACCTGGAG TTCTTTGTCA AGCTGCCCAG TCTAAGGCTC 
CTGGACATTT CGTTTAACAA CTTGGACTCC ATTCCAGCTC AGGTCATTGA AACCTTGCCT 
GGAAGCTTGA GGAAATTGTC CATTGCCTGT AACAATCTCT ACGACTTCCC CTGGGACAAA 
ATGGAAAAGC TGAGCAGCTT GGAATTCCTA GACTTGAGTT ATAATGCACT GGAGACGCTG 
CTGGTCGTGG GCAGAGGCTC CTTTGGCAAG AACCTCACCA CCCTCGACCT CAGCTACAAC 
AAAATCAAGT CTCTCCAGGA AGCGTTCTTT TCCCACGCCA GTTCCTTGCA CTTTCTCAGT 
TTGAACCATA ACAAGATCCA GATGGTCGAT GAAAGCAGCT TTCCCAAAGC CCTTCTTCAG 
AGCTTGCAGC AGCTGGACGT GAGCAGCAAT CCCTTGCGGT GCACCTGTGC AGCCCACTGG 
TTCCTCACCT TCCTCAAAAG CACGTCCATC ACCATAGACC ATTTCTCCAC TAGCATGCAA 
TGTGACCTGC CCGAATCAAA GCGGGGGCAG CCTCTGCTCT CCATGGACCC TCGGTCCTGC 
CAGGATATAT ACGGTCACCT AAGCTTCCTG TGCTCTTCCC TCCTGGTGAC CTTGGTGACT 
GTGCTTCCAG TCCTCCATAA GTTGTATGGC TGGGACCTCT GGTATGTCAG TCACCTCTGC 
CTGGCCACAC TCAGACAAGG CTATGCCCAG GTGGCCACAG CCTCCCGGGA GGAATACGAT 
GCATTCGTAG CCTTTGACGC CCAACACAGC CTGGTGGCTG ACTGGGTCTA CAATGAGATG 
GTGCCCCAGC TGGAGGAGAA AGGAAGAAGG AGATTCAAGC TGTGCCTGGA GGAAAGGGAC 
TGGACAGTGG GCGGGTCCAG GATTGAGAAC CTCTGTGACT CCATCTACAA GAGCAGGAAA 
ACCATCTTCA TCCTGGGGAG AGAAGGGTTT GGCAGCGGCT TCCTGCGGCA CACCTTCCTC 
CTCAGCCAGC AGCGGCTGCT GGACGAGAAG GTAGATGTGC TCCTGCTGGT GCTACTGGAC 
CCCGCGATGA AGATGTCCAG GTTCCTGCTG GCCCGGAGAA GACTGTGTGG GAGGACAGTC 
CTGGACTGGC CCTGCAACCC CTTTGCCCAG CCCTACTTCT GGCACTGCGT GCGAGCCCGG 
CTGGCCTGCG AGGTTCATCC CTTTGATGCC TCACACCTGA GGAGAGGCGT CAGCAGTTCT 
GGGGCAGGGG AGCTCCTGCC AGCAGAGGAC GCCATGGCCA GGCCGGCTTT GCAAAGGCCC 
CGAGAAGCAG CCAGGGCTTG CGCATGGCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102444127 ( XM_014571799.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014427285.1 Pelodiscus sinensis toll-like receptor 9 (LOC102444127), mRNA.

Price & Availability↑

CloneID	OPe26198
Clone ID Related Accession (Same CDS sequence)	XM_014571799.1
Accession Version	XM_014571799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	toll-like receptor 9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854103.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGT GGCGGGTGTG TCTGCTGGCC CAGGGCCTCC TGGGGGCCGG CGCCCTCTTC 
CCTAGTTACC CGCCCTGCGC TAACAGCAAA GACGGCAAGA ACGTGGACTG CAGCCTGCGC 
AGCCTCACCC TGGTGCCAGA CATACGTTCC AGCAGCGTGA CCTCCCTGAA CCTGGACAGC 
AACTTCCTAG CCCTGCTGGC CAATGGCTCC TTCCAGGGCG TCCCTTGCCT GAGGGATCTC 
TCGCTCAGCT GGAACTGCAT GCCGGGGCAG CTGCGGAAAG CGCACGCGGT GTGTCAGCTG 
GTGATCCAGC CAGGGGCCCT GACCGTGCTG CGCCACCTCA CCAACCTGAG CCTGGCAGGA 
AACAGCCTGA GCGTGCTGCC CCCGCTGCCC TGCACCCTCC GCTTCCTGGA CTTGAGTTAC 
AACAACATCC TCAGGCTGGG CCCTCGGAGC CTGGTGGAGC TCAGCCACGT GAGCGAGCTT 
CTCCTAGGCA ACAACTGCTA CTACCGGAAC CCGTGCGGCT CCTCCGTCAG CCTCGCACGA 
GATGCCTTCC TGCCCCTGAC CTCCCTGAGG CGGCTCTCGC TGAAGTTTAA CAACATGACT 
GCAACTCCCC AGGGGCTCCC GCCCACGCTG CACCAGCTCG ACCTCTCGGA GAACAAGATC 
GCCCACGTGA GCCACCTGGA GAACCTCACC AGCCTGAGGT CCCTCAACCT GGAGTGGAAC 
TGCCAGCGCT GTGACCATGC TGCTCAGCCC TGCTTCCCCT GCCCAGGCAA CAAGGCCCTG 
ACCTTCGAGC AGGATGCTTT CCAGAACCTC GCGCAGCTCA GGAGCTTGAA CCTGCGAGGC 
AACTCCCTGC AGGACCTCAA CGCCAGCTTC TTCAGCAGCC TCCGCAACTT AGAGACCTTG 
GATCTGTCAG ACAACTTCCT GACCTGCGCC ATTAGACATG GCACCTTCTT CTCCGTGCTG 
AGCTCAGTGC AGACCCTAAA CCTGGGCTAC AATTACCAGT CACTGGTGAC CTTCGAGAAT 
CTGACTCTGT CTGACTCCTT CGGCCAAATG ACATCTCTGA AAAATCTCAT CATCAATGGC 
TACTTCTTCA GGCACTTGGC TGAGGACGGG ATCAAGCCTC TCTTCAACCT GAGCAACTTG 
GAGACCCTCC AATTTCGAAC CAACTTCATC AGGAACGTGA ATCTGTCCCT CTTTTCCAAG 
TGCAGGACTC TCACGTTCAT CAGTCTGTCC CAGAACAACA TTGTCTTCTC CCCCCCATGC 
GAGAGAGGTG GGAATGGAGC TGGAACGGGA CCTCGGGGCC TCTCCCATGA TGGCACTTTG 
GTGGGGAATC CCAGACGCTG GGCGGAACCC AAAGGAAGAG CTGAGGCCAT GGTGAGGGAC 
AGCCAAAGCT CCCATGCCAA CCTCCCGGCA GGCGTTCGCT GCAGCAAGAC CCTGGATTTA 
TCATTCAATA GCATCCACAG CATTGAGCCA GCCCAGTTCC AAGGGCTAGA GGCAATTGAC 
TGCCTCAACC TCAGCTCTAA TTACATCAGC CAGTCGCTAA ATGGCAGCCA GTTCCTGCCG 
CTGCGGTCCC TGAAGCTGCT GGATCTGTCT CACAATAGGT TCGACCTGTA CTATGACAGC 
GCCCTGCAAG AGGTCTGCCA GCTGGTCGTG CTGAACCTCA GCAATAATCA ATTCCACTTC 
ACAATCAAAG GCCTGGGGCA CAAGTTTGAG TTCTTGAAAA ACCTCACCAA CTTGAAATTT 
CTGAACCTGG ACAACAACCA GATCGGTATC AGGATCTCCA AACACCTGGA AAGCGCTGGA 
CATCATCTGC CCAGTCTAAG GCTCCTGGAC ATTTCGTTTA ACAACTTGGA CATCATGTGG 
ATGAGCGGGA GGAATACCTA CCTGGAGTTC TTTGTCAAGC TGCCCAGTCT AAGGCTCCTG 
GACATTTCGT TTAACAACTT GGACTCCATT CCAGCTCAGG TCATTGAAAC CTTGCCTGGA 
AGCTTGAGGA AATTGTCCAT TGCCTGTAAC AATCTCTACG ACTTCCCCTG GGACAAAATG 
GAAAAGCTGA GCAGCTTGGA ATTCCTAGAC TTGAGTTATA ATGCACTGGA GACGCTGCTG 
GTCGTGGGCA GAGGCTCCTT TGGCAAGAAC CTCACCACCC TCGACCTCAG CTACAACAAA 
ATCAAGTCTC TCCAGGAAGC GTTCTTTTCC CACGCCAGTT CCTTGCACTT TCTCAGTTTG 
AACCATAACA AGATCCAGAT GGTCGATGAA AGCAGCTTTC CCAAAGCCCT TCTTCAGAGC 
TTGCAGCAGC TGGACGTGAG CAGCAATCCC TTGCGGTGCA CCTGTGCAGC CCACTGGTTC 
CTCACCTTCC TCAAAAGCAC GTCCATCACC ATAGACCATT TCTCCACTAG CATGCAATGT 
GACCTGCCCG AATCAAAGCG GGGGCAGCCT CTGCTCTCCA TGGACCCTCG GTCCTGCCAG 
GATATATACG GTCACCTAAG CTTCCTGTGC TCTTCCCTCC TGGTGACCTT GGTGACTGTG 
CTTCCAGTCC TCCATAAGTT GTATGGCTGG GACCTCTGGT ATGTCAGTCA CCTCTGCCTG 
GCCACACTCA GACAAGGCTA TGCCCAGGTG GCCACAGCCT CCCGGGAGGA ATACGATGCA 
TTCGTAGCCT TTGACGCCCA ACACAGCCTG GTGGCTGACT GGGTCTACAA TGAGATGGTG 
CCCCAGCTGG AGGAGAAAGG AAGAAGGAGA TTCAAGCTGT GCCTGGAGGA AAGGGACTGG 
ACAGTGGGCG GGTCCAGGAT TGAGAACCTC TGTGACTCCA TCTACAAGAG CAGGAAAACC 
ATCTTCATCC TGGGGAGAGA AGGGTTTGGC AGCGGCTTCC TGCGGCACAC CTTCCTCCTC 
AGCCAGCAGC GGCTGCTGGA CGAGAAGGTA GATGTGCTCC TGCTGGTGCT ACTGGACCCC 
GCGATGAAGA TGTCCAGGTT CCTGCTGGCC CGGAGAAGAC TGTGTGGGAG GACAGTCCTG 
GACTGGCCCT GCAACCCCTT TGCCCAGCCC TACTTCTGGC ACTGCGTGCG AGCCCGGCTG 
GCCTGCGAGG TTCATCCCTT TGATGCCTCA CACCTGAGGA GAGGCGTCAG CAGTTCTGGG 
GCAGGGGAGC TCCTGCCAGC AGAGGACGCC ATGGCCAGGC CGGCTTTGCA AAGGCCCCGA 
GAAGCAGCCA GGGCTTGCGC ATGGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014427285.1
CDS	77..3286
Translation	MLLWRVCLLAQGLLGAGALFPSYPPCANSKDGKNVDCSLRSLTLVPDIRSSSVTSLNLDSNFLALLANGSFQGVPCLRDLSLSWNCMPGQLRKAHAVCQLVIQPGALTVLRHLTNLSLAGNSLSVLPPLPCTLRFLDLSYNNILRLGPRSLVELSHVSELLLGNNCYYRNPCGSSVSLARDAFLPLTSLRRLSLKFNNMTATPQGLPPTLHQLDLSENKIAHVSHLENLTSLRSLNLEWNCQRCDHAAQPCFPCPGNKALTFEQDAFQNLAQLRSLNLRGNSLQDLNASFFSSLRNLETLDLSDNFLTCAIRHGTFFSVLSSVQTLNLGYNYQSLVTFENLTLSDSFGQMTSLKNLIINGYFFRHLAEDGIKPLFNLSNLETLQFRTNFIRNVNLSLFSKCRTLTFISLSQNNIVFSPPCERGGNGAGTGPRGLSHDGTLVGNPRRWAEPKGRAEAMVRDSQSSHANLPAGVRCSKTLDLSFNSIHSIEPAQFQGLEAIDCLNLSSNYISQSLNGSQFLPLRSLKLLDLSHNRFDLYYDSALQEVCQLVVLNLSNNQFHFTIKGLGHKFEFLKNLTNLKFLNLDNNQIGIRISKHLESAGHHLPSLRLLDISFNNLDIMWMSGRNTYLEFFVKLPSLRLLDISFNNLDSIPAQVIETLPGSLRKLSIACNNLYDFPWDKMEKLSSLEFLDLSYNALETLLVVGRGSFGKNLTTLDLSYNKIKSLQEAFFSHASSLHFLSLNHNKIQMVDESSFPKALLQSLQQLDVSSNPLRCTCAAHWFLTFLKSTSITIDHFSTSMQCDLPESKRGQPLLSMDPRSCQDIYGHLSFLCSSLLVTLVTVLPVLHKLYGWDLWYVSHLCLATLRQGYAQVATASREEYDAFVAFDAQHSLVADWVYNEMVPQLEEKGRRRFKLCLEERDWTVGGSRIENLCDSIYKSRKTIFILGREGFGSGFLRHTFLLSQQRLLDEKVDVLLLVLLDPAMKMSRFLLARRRLCGRTVLDWPCNPFAQPYFWHCVRARLACEVHPFDASHLRRGVSSSGAGELLPAEDAMARPALQRPREAARACAWQ

Target ORF information:

RefSeq Version	XM_014571799.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis toll-like receptor 9 (LOC102444127), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014571799.1

ORF Insert Sequence:

ATGCTGCTGT GGCGGGTGTG TCTGCTGGCC CAGGGCCTCC TGGGGGCCGG CGCCCTCTTC 
CCTAGTTACC CGCCCTGCGC TAACAGCAAA GACGGCAAGA ACGTGGACTG CAGCCTGCGC 
AGCCTCACCC TGGTGCCAGA CATACGTTCC AGCAGCGTGA CCTCCCTGAA CCTGGACAGC 
AACTTCCTAG CCCTGCTGGC CAATGGCTCC TTCCAGGGCG TCCCTTGCCT GAGGGATCTC 
TCGCTCAGCT GGAACTGCAT GCCGGGGCAG CTGCGGAAAG CGCACGCGGT GTGTCAGCTG 
GTGATCCAGC CAGGGGCCCT GACCGTGCTG CGCCACCTCA CCAACCTGAG CCTGGCAGGA 
AACAGCCTGA GCGTGCTGCC CCCGCTGCCC TGCACCCTCC GCTTCCTGGA CTTGAGTTAC 
AACAACATCC TCAGGCTGGG CCCTCGGAGC CTGGTGGAGC TCAGCCACGT GAGCGAGCTT 
CTCCTAGGCA ACAACTGCTA CTACCGGAAC CCGTGCGGCT CCTCCGTCAG CCTCGCACGA 
GATGCCTTCC TGCCCCTGAC CTCCCTGAGG CGGCTCTCGC TGAAGTTTAA CAACATGACT 
GCAACTCCCC AGGGGCTCCC GCCCACGCTG CACCAGCTCG ACCTCTCGGA GAACAAGATC 
GCCCACGTGA GCCACCTGGA GAACCTCACC AGCCTGAGGT CCCTCAACCT GGAGTGGAAC 
TGCCAGCGCT GTGACCATGC TGCTCAGCCC TGCTTCCCCT GCCCAGGCAA CAAGGCCCTG 
ACCTTCGAGC AGGATGCTTT CCAGAACCTC GCGCAGCTCA GGAGCTTGAA CCTGCGAGGC 
AACTCCCTGC AGGACCTCAA CGCCAGCTTC TTCAGCAGCC TCCGCAACTT AGAGACCTTG 
GATCTGTCAG ACAACTTCCT GACCTGCGCC ATTAGACATG GCACCTTCTT CTCCGTGCTG 
AGCTCAGTGC AGACCCTAAA CCTGGGCTAC AATTACCAGT CACTGGTGAC CTTCGAGAAT 
CTGACTCTGT CTGACTCCTT CGGCCAAATG ACATCTCTGA AAAATCTCAT CATCAATGGC 
TACTTCTTCA GGCACTTGGC TGAGGACGGG ATCAAGCCTC TCTTCAACCT GAGCAACTTG 
GAGACCCTCC AATTTCGAAC CAACTTCATC AGGAACGTGA ATCTGTCCCT CTTTTCCAAG 
TGCAGGACTC TCACGTTCAT CAGTCTGTCC CAGAACAACA TTGTCTTCTC CCCCCCATGC 
GAGAGAGGTG GGAATGGAGC TGGAACGGGA CCTCGGGGCC TCTCCCATGA TGGCACTTTG 
GTGGGGAATC CCAGACGCTG GGCGGAACCC AAAGGAAGAG CTGAGGCCAT GGTGAGGGAC 
AGCCAAAGCT CCCATGCCAA CCTCCCGGCA GGCGTTCGCT GCAGCAAGAC CCTGGATTTA 
TCATTCAATA GCATCCACAG CATTGAGCCA GCCCAGTTCC AAGGGCTAGA GGCAATTGAC 
TGCCTCAACC TCAGCTCTAA TTACATCAGC CAGTCGCTAA ATGGCAGCCA GTTCCTGCCG 
CTGCGGTCCC TGAAGCTGCT GGATCTGTCT CACAATAGGT TCGACCTGTA CTATGACAGC 
GCCCTGCAAG AGGTCTGCCA GCTGGTCGTG CTGAACCTCA GCAATAATCA ATTCCACTTC 
ACAATCAAAG GCCTGGGGCA CAAGTTTGAG TTCTTGAAAA ACCTCACCAA CTTGAAATTT 
CTGAACCTGG ACAACAACCA GATCGGTATC AGGATCTCCA AACACCTGGA AAGCGCTGGA 
CATCATCTGC CCAGTCTAAG GCTCCTGGAC ATTTCGTTTA ACAACTTGGA CATCATGTGG 
ATGAGCGGGA GGAATACCTA CCTGGAGTTC TTTGTCAAGC TGCCCAGTCT AAGGCTCCTG 
GACATTTCGT TTAACAACTT GGACTCCATT CCAGCTCAGG TCATTGAAAC CTTGCCTGGA 
AGCTTGAGGA AATTGTCCAT TGCCTGTAAC AATCTCTACG ACTTCCCCTG GGACAAAATG 
GAAAAGCTGA GCAGCTTGGA ATTCCTAGAC TTGAGTTATA ATGCACTGGA GACGCTGCTG 
GTCGTGGGCA GAGGCTCCTT TGGCAAGAAC CTCACCACCC TCGACCTCAG CTACAACAAA 
ATCAAGTCTC TCCAGGAAGC GTTCTTTTCC CACGCCAGTT CCTTGCACTT TCTCAGTTTG 
AACCATAACA AGATCCAGAT GGTCGATGAA AGCAGCTTTC CCAAAGCCCT TCTTCAGAGC 
TTGCAGCAGC TGGACGTGAG CAGCAATCCC TTGCGGTGCA CCTGTGCAGC CCACTGGTTC 
CTCACCTTCC TCAAAAGCAC GTCCATCACC ATAGACCATT TCTCCACTAG CATGCAATGT 
GACCTGCCCG AATCAAAGCG GGGGCAGCCT CTGCTCTCCA TGGACCCTCG GTCCTGCCAG 
GATATATACG GTCACCTAAG CTTCCTGTGC TCTTCCCTCC TGGTGACCTT GGTGACTGTG 
CTTCCAGTCC TCCATAAGTT GTATGGCTGG GACCTCTGGT ATGTCAGTCA CCTCTGCCTG 
GCCACACTCA GACAAGGCTA TGCCCAGGTG GCCACAGCCT CCCGGGAGGA ATACGATGCA 
TTCGTAGCCT TTGACGCCCA ACACAGCCTG GTGGCTGACT GGGTCTACAA TGAGATGGTG 
CCCCAGCTGG AGGAGAAAGG AAGAAGGAGA TTCAAGCTGT GCCTGGAGGA AAGGGACTGG 
ACAGTGGGCG GGTCCAGGAT TGAGAACCTC TGTGACTCCA TCTACAAGAG CAGGAAAACC 
ATCTTCATCC TGGGGAGAGA AGGGTTTGGC AGCGGCTTCC TGCGGCACAC CTTCCTCCTC 
AGCCAGCAGC GGCTGCTGGA CGAGAAGGTA GATGTGCTCC TGCTGGTGCT ACTGGACCCC 
GCGATGAAGA TGTCCAGGTT CCTGCTGGCC CGGAGAAGAC TGTGTGGGAG GACAGTCCTG 
GACTGGCCCT GCAACCCCTT TGCCCAGCCC TACTTCTGGC ACTGCGTGCG AGCCCGGCTG 
GCCTGCGAGG TTCATCCCTT TGATGCCTCA CACCTGAGGA GAGGCGTCAG CAGTTCTGGG 
GCAGGGGAGC TCCTGCCAGC AGAGGACGCC ATGGCCAGGC CGGCTTTGCA AAGGCCCCGA 
GAAGCAGCCA GGGCTTGCGC ATGGCAATGA