APAF1 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	APAF1
Entrez Gene ID	110084192
Full Name	apoptotic peptidase activating factor 1
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020803318.1	XP_020658977.1	apoptotic protease-activating factor 1

Pathways from BioSystems
Gene Ontology

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo249869	XM_020803318.1 Latest version!	Pogona vitticeps apoptotic peptidase activating factor 1 (APAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APAF1 ( XM_020803318.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020658977.1 Pogona vitticeps apoptotic peptidase activating factor 1 (APAF1), mRNA.

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CloneID	OPo249869
Clone ID Related Accession (Same CDS sequence)	XM_020803318.1
Accession Version	XM_020803318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	apoptotic protease-activating factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151041.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGATTTGA AGGCTCGAAG TTACTTGCTT CGGAATCGAG AGGCTCTGGA AAGAGATATC 
AAAATTTCCT ATATAATGGA CCATATGATT ACAGATGGAG TATTAACTTT GCTGGAAGAG 
GAGAAAGTAA AAGCTGAGAC AACACAAAGA GAAAGAGCAG CTATGCTGGT AAAACTAATT 
CTTGCTAAAG ATGATTATGC ATACATATCT TTTTACAATG CTCTGGTTCA TGAGGGGTAC 
AAAGATCTTG CTGCCCTTCT CCAGGACGGT ATCCCTATTA TTTCTCCTTA TAATGGAAAG 
AATTCTATGG ATGGAGAAAC CTCATACGTT AAGACTGTTC TCTGTGAAGG TGGAGTTCCA 
CAACGACCAG TGGTGTTTGT CACCCGCCCA GAATTAGCAA AGAGAATTCA AGAGAAACTA 
CATAAATTGA AAAGTGATCC AGGTTGGGTT GTTGTATATG GCATGGCAGG CTGTGGAAAG 
TCTGTTTTGG CAGCAGAAGC ACTACGTGAC CACAGTCTTT TGAAAGATTG CTTCCCAGGA 
GGAGTACACT GGGTTTCTGT TGGAAAACAA GACAAGGCAG GTCTGTTAAT GAAGCTCCAA 
AATCTGTGTC TGAGATTAGA CCAGGATTTC ACTTATTCAC AGAGGCCACC ACTTAATATT 
GAGGAAGCAA AAGATCGCCT CCGTTCATTG CTGTTGCGCA AATATCCAGG ATCTCTCTTG 
GTGCTGGATG ATATTTGGGA TCCGTGGGTA CTAAAAGCCT TTGACAGTCA GTTCCAGATT 
CTTATCACCA GTAGGGACAG GAGTGTAACA GACTCTGTAA CTGGCAAAAA ATATAAAGTT 
CCTGTGGAAA GTGGACTAAC ACATGAAAAG GCACTTGAAA TATTATCCTT ATTTGTTAAC 
ATGGAAATAA CTGAGTTGCC AGAAAAAGCT GATAGTATTG TAAGAGAATG CAAAGGTTCT 
CCTCTTGTAG TGTCTTTGAT TGGAGCATTA TTACGAGACT TCCCCAATCG CTGGGAATAT 
TACCTTAAAC AACTTCAAAA CAAACAGTTC AAAAGAATAA GGAAATCATC ATCTTATGAT 
TATGAAGCAC TTGATGAAGC AATGTCTATC AGTGTTGAGA TGTTGCCAGA AAATTTTAAA 
GACTATTACA GAGATCTTTC AGTCCTTCAG AAGGATGTTA AAGTGCCTTC AAAGGTATTC 
TGTATTCTTT GGGATATGGA GACAGAAGAA GTTGAGGACA TACTGCAAGA ATTCATTAAC 
AAATCTCTCT TATTCTGTGA TCGGAATGGG AAATCGTTCC ATTATTATTT ACATGATCTT 
CAGCTTGATT TTCTGACTGA GAAGAATCAT GGTCAGCTTC AAACACTACA TAAAAAAGTA 
GTTCATCAAT ACAAGAAGCA TTATAAACAG TATGTACCAA CTTCAACACA AGAAGATTGC 
ATTTACTGGT ATTATTTTCT GGCGTACCAT ATGGCAAGTG CCAACATGCA AAATGAACTC 
AGTGATCTCA TGTTTTCACT AGATTGGATT AAAACCAAAA CAGAATTGGT AGGACCTGCT 
CATTTGATTC ATGAGTATGT TGAATACAGT CACATACTAG ATCAGAAGGA TACTATTGTA 
CGTGAGAACT TCCATGAATT TCTGTCTTTA AATGGACATC TCCTTGGGCG GCAGCCATCC 
CTAGACATTG TACAACTTGG ACTATGTCAG CCAGAAAGCT CAGAAGTATA TCGACAAGCA 
AAACTAAAAG CTCTGCAAGA ACTGAATAAA GGATCCTTTT ATGTGGAATG GATAAATAAA 
AAATCCATAA AGAACATGTA TCGTCTGGTT GTGCGTCCTC ATACAGATGC TGTTTACCAT 
GCTTGTTTTT CCCAGGACAG ACAGAGAATA GCTTCATGTG GAGCAGATAA AACATTGCAG 
ATATTTAAAG CTGAAAGTGG AGAGAAACTT CTGGAAGTAA AAGCTCATGA TGATGAAGTG 
TTGTGTTGTG CTTTTTCAGC AGATGACAGA TTTGTTGCAA CTTGTTCCGC GGATAAAAAA 
GTAAAAATCT GGAATTCAAG AACAGGTCAA CTTGTGTGCT TATTTGAAGA ACATGTAGAA 
GAGGTCAACT GCTGCCAGTT TAACAAAACA AGTAGTCACC AATTTCTCTT AGTCACGTGT 
TCAAATGACA GGTTTGTCAA ACTCTGGGAT GTGAACAAGA AATACTGTCG TAATACATTG 
TTTGGCCACA GGAATTCTGT TACTCACTGC AGATTTTCAC CAGATGATAA ATATGTGGCT 
AGCTGTTCAG CAGATGGAAC TTTAAAGCTT TGGCATGTAC AGTCAGCAAA CGAACTAAAA 
ACTATTGACA TAGGTGACTT CTTCAGAAAT GTGGAAGATT ATCAGACTGA TGGAGAATTG 
TTAGTAAAGT GTTGTTCCTG GTCATCTAAT GGAGCCATAA TTGTGGCGGC AGCCAAAAAT 
AAGCTTTTTA TTTTTGACGT TAAGTCCAGT GATTTGTTGG CAAATATCGT TATAAGTCAC 
CACAATACTA TTCAGTATTG TGACTTTTGT CCAAACAGTC AGATGGTAGC AGTTGCTCTA 
TCACATTATT CTGTTGAGAT ATGGGATATT GATTCCTTGT CCAAAGTAGC TGACTGCCGA 
GGACATATGA GCTGGGTGCA TTCTGTGTCA TTTTCTTTTG ATGGATCTTT ATTTCTGACT 
TCATCTGATG ACCAGACAAT AAGGATTTGG GAGACAAGTA AAGTATTCAA GTCCTCTGCC 
ATTGTATTAA AACGTGAAAT AGATGTTTTG TTTCCAGACA ATGAGGCAAT CATTTTAGCC 
ATTGACAGTT CAAAGTATCT GCAACTAATC AATGGAAACA CAGGCCATAC AGTTTATCTG 
ACTGAAACGC AGGCATCATG CATTTGCTGT TGTTGCCTAA GTGAAGATCT GCAGGTTGCT 
GCATTTGGAC AGAAGAATGG AACAGTAAAG GTAATAAACC TGCCAAATGG AAATGTTTTA 
AAATCCAGAA CTGGACACAC AGAAGCTGTG CAACATTGTC AGTTTACTTC TGATGGAAAC 
ACTCTTGTTT CAAGCTCAGA TGACTCTACT ATACAGGTGT GGAATTGGCA GTCAGAGGAA 
TGCATTTTTC TCAAAGGCCA CAATGAACCT GTCAAAAACT TTTCTCTTTT GAAACATTTA 
AGGCTTTTTT CATGGTCATT TGATGGGACA GTTAAGCTCT GGAACACAGT TAGTGGAGAA 
CTGGAAAGAG ATCTTACTTG CCATGAAGAT GCAATTCTTT CTTGTGCTAT TTCACCTGAT 
GGCACTATGT TTTCCACTGC ATCTGCTGAC AAGACTGCTA AGATATGGCG TTTTGAAAGT 
TTGTCAATTC TTTATAAACT AAATAGTCAC AAAAGCTGTG TGAGATGTTG TGCTTTCTCC 
AGTAATAATG AATTATTGGC CACAGGAGAT GACAATGGAG AAATAAGGAT ATGGCGTTTT 
GAAAGTTTGT CAATTCTTCA TAAACTAAAT AGTCACAAAA GCTGTGTGAG ATGTTGTGCT 
TTCTCCAGTA ATAATGAATT ATTGGCCACA GGAGATGACA ATGGAGAAAT AAGGTGGTGG 
AATGTGGATA CTGGCCAGCC TCTACAAACA TTCTACACAA ATGGAACCAA TCTGAAATCA 
TTGCATGTAT CCCCAGATTT CAGAGTATGT GTAACTGTAG ATAACCTTGG CATTCTTTAT 
GTGCTACAGA TTATGGAAAA ATCTTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020658977.1
CDS	321..4070
Translation	MDLKARSYLLRNREALERDIKISYIMDHMITDGVLTLLEEEKVKAETTQRERAAMLVKLILAKDDYAYISFYNALVHEGYKDLAALLQDGIPIISPYNGKNSMDGETSYVKTVLCEGGVPQRPVVFVTRPELAKRIQEKLHKLKSDPGWVVVYGMAGCGKSVLAAEALRDHSLLKDCFPGGVHWVSVGKQDKAGLLMKLQNLCLRLDQDFTYSQRPPLNIEEAKDRLRSLLLRKYPGSLLVLDDIWDPWVLKAFDSQFQILITSRDRSVTDSVTGKKYKVPVESGLTHEKALEILSLFVNMEITELPEKADSIVRECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLPENFKDYYRDLSVLQKDVKVPSKVFCILWDMETEEVEDILQEFINKSLLFCDRNGKSFHYYLHDLQLDFLTEKNHGQLQTLHKKVVHQYKKHYKQYVPTSTQEDCIYWYYFLAYHMASANMQNELSDLMFSLDWIKTKTELVGPAHLIHEYVEYSHILDQKDTIVRENFHEFLSLNGHLLGRQPSLDIVQLGLCQPESSEVYRQAKLKALQELNKGSFYVEWINKKSIKNMYRLVVRPHTDAVYHACFSQDRQRIASCGADKTLQIFKAESGEKLLEVKAHDDEVLCCAFSADDRFVATCSADKKVKIWNSRTGQLVCLFEEHVEEVNCCQFNKTSSHQFLLVTCSNDRFVKLWDVNKKYCRNTLFGHRNSVTHCRFSPDDKYVASCSADGTLKLWHVQSANELKTIDIGDFFRNVEDYQTDGELLVKCCSWSSNGAIIVAAAKNKLFIFDVKSSDLLANIVISHHNTIQYCDFCPNSQMVAVALSHYSVEIWDIDSLSKVADCRGHMSWVHSVSFSFDGSLFLTSSDDQTIRIWETSKVFKSSAIVLKREIDVLFPDNEAIILAIDSSKYLQLINGNTGHTVYLTETQASCICCCCLSEDLQVAAFGQKNGTVKVINLPNGNVLKSRTGHTEAVQHCQFTSDGNTLVSSSDDSTIQVWNWQSEECIFLKGHNEPVKNFSLLKHLRLFSWSFDGTVKLWNTVSGELERDLTCHEDAILSCAISPDGTMFSTASADKTAKIWRFESLSILYKLNSHKSCVRCCAFSSNNELLATGDDNGEIRIWRFESLSILHKLNSHKSCVRCCAFSSNNELLATGDDNGEIRWWNVDTGQPLQTFYTNGTNLKSLHVSPDFRVCVTVDNLGILYVLQIMEKSF

Target ORF information:

RefSeq Version	XM_020803318.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps apoptotic peptidase activating factor 1 (APAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020803318.1

ORF Insert Sequence:

ATGGATTTGA AGGCTCGAAG TTACTTGCTT CGGAATCGAG AGGCTCTGGA AAGAGATATC 
AAAATTTCCT ATATAATGGA CCATATGATT ACAGATGGAG TATTAACTTT GCTGGAAGAG 
GAGAAAGTAA AAGCTGAGAC AACACAAAGA GAAAGAGCAG CTATGCTGGT AAAACTAATT 
CTTGCTAAAG ATGATTATGC ATACATATCT TTTTACAATG CTCTGGTTCA TGAGGGGTAC 
AAAGATCTTG CTGCCCTTCT CCAGGACGGT ATCCCTATTA TTTCTCCTTA TAATGGAAAG 
AATTCTATGG ATGGAGAAAC CTCATACGTT AAGACTGTTC TCTGTGAAGG TGGAGTTCCA 
CAACGACCAG TGGTGTTTGT CACCCGCCCA GAATTAGCAA AGAGAATTCA AGAGAAACTA 
CATAAATTGA AAAGTGATCC AGGTTGGGTT GTTGTATATG GCATGGCAGG CTGTGGAAAG 
TCTGTTTTGG CAGCAGAAGC ACTACGTGAC CACAGTCTTT TGAAAGATTG CTTCCCAGGA 
GGAGTACACT GGGTTTCTGT TGGAAAACAA GACAAGGCAG GTCTGTTAAT GAAGCTCCAA 
AATCTGTGTC TGAGATTAGA CCAGGATTTC ACTTATTCAC AGAGGCCACC ACTTAATATT 
GAGGAAGCAA AAGATCGCCT CCGTTCATTG CTGTTGCGCA AATATCCAGG ATCTCTCTTG 
GTGCTGGATG ATATTTGGGA TCCGTGGGTA CTAAAAGCCT TTGACAGTCA GTTCCAGATT 
CTTATCACCA GTAGGGACAG GAGTGTAACA GACTCTGTAA CTGGCAAAAA ATATAAAGTT 
CCTGTGGAAA GTGGACTAAC ACATGAAAAG GCACTTGAAA TATTATCCTT ATTTGTTAAC 
ATGGAAATAA CTGAGTTGCC AGAAAAAGCT GATAGTATTG TAAGAGAATG CAAAGGTTCT 
CCTCTTGTAG TGTCTTTGAT TGGAGCATTA TTACGAGACT TCCCCAATCG CTGGGAATAT 
TACCTTAAAC AACTTCAAAA CAAACAGTTC AAAAGAATAA GGAAATCATC ATCTTATGAT 
TATGAAGCAC TTGATGAAGC AATGTCTATC AGTGTTGAGA TGTTGCCAGA AAATTTTAAA 
GACTATTACA GAGATCTTTC AGTCCTTCAG AAGGATGTTA AAGTGCCTTC AAAGGTATTC 
TGTATTCTTT GGGATATGGA GACAGAAGAA GTTGAGGACA TACTGCAAGA ATTCATTAAC 
AAATCTCTCT TATTCTGTGA TCGGAATGGG AAATCGTTCC ATTATTATTT ACATGATCTT 
CAGCTTGATT TTCTGACTGA GAAGAATCAT GGTCAGCTTC AAACACTACA TAAAAAAGTA 
GTTCATCAAT ACAAGAAGCA TTATAAACAG TATGTACCAA CTTCAACACA AGAAGATTGC 
ATTTACTGGT ATTATTTTCT GGCGTACCAT ATGGCAAGTG CCAACATGCA AAATGAACTC 
AGTGATCTCA TGTTTTCACT AGATTGGATT AAAACCAAAA CAGAATTGGT AGGACCTGCT 
CATTTGATTC ATGAGTATGT TGAATACAGT CACATACTAG ATCAGAAGGA TACTATTGTA 
CGTGAGAACT TCCATGAATT TCTGTCTTTA AATGGACATC TCCTTGGGCG GCAGCCATCC 
CTAGACATTG TACAACTTGG ACTATGTCAG CCAGAAAGCT CAGAAGTATA TCGACAAGCA 
AAACTAAAAG CTCTGCAAGA ACTGAATAAA GGATCCTTTT ATGTGGAATG GATAAATAAA 
AAATCCATAA AGAACATGTA TCGTCTGGTT GTGCGTCCTC ATACAGATGC TGTTTACCAT 
GCTTGTTTTT CCCAGGACAG ACAGAGAATA GCTTCATGTG GAGCAGATAA AACATTGCAG 
ATATTTAAAG CTGAAAGTGG AGAGAAACTT CTGGAAGTAA AAGCTCATGA TGATGAAGTG 
TTGTGTTGTG CTTTTTCAGC AGATGACAGA TTTGTTGCAA CTTGTTCCGC GGATAAAAAA 
GTAAAAATCT GGAATTCAAG AACAGGTCAA CTTGTGTGCT TATTTGAAGA ACATGTAGAA 
GAGGTCAACT GCTGCCAGTT TAACAAAACA AGTAGTCACC AATTTCTCTT AGTCACGTGT 
TCAAATGACA GGTTTGTCAA ACTCTGGGAT GTGAACAAGA AATACTGTCG TAATACATTG 
TTTGGCCACA GGAATTCTGT TACTCACTGC AGATTTTCAC CAGATGATAA ATATGTGGCT 
AGCTGTTCAG CAGATGGAAC TTTAAAGCTT TGGCATGTAC AGTCAGCAAA CGAACTAAAA 
ACTATTGACA TAGGTGACTT CTTCAGAAAT GTGGAAGATT ATCAGACTGA TGGAGAATTG 
TTAGTAAAGT GTTGTTCCTG GTCATCTAAT GGAGCCATAA TTGTGGCGGC AGCCAAAAAT 
AAGCTTTTTA TTTTTGACGT TAAGTCCAGT GATTTGTTGG CAAATATCGT TATAAGTCAC 
CACAATACTA TTCAGTATTG TGACTTTTGT CCAAACAGTC AGATGGTAGC AGTTGCTCTA 
TCACATTATT CTGTTGAGAT ATGGGATATT GATTCCTTGT CCAAAGTAGC TGACTGCCGA 
GGACATATGA GCTGGGTGCA TTCTGTGTCA TTTTCTTTTG ATGGATCTTT ATTTCTGACT 
TCATCTGATG ACCAGACAAT AAGGATTTGG GAGACAAGTA AAGTATTCAA GTCCTCTGCC 
ATTGTATTAA AACGTGAAAT AGATGTTTTG TTTCCAGACA ATGAGGCAAT CATTTTAGCC 
ATTGACAGTT CAAAGTATCT GCAACTAATC AATGGAAACA CAGGCCATAC AGTTTATCTG 
ACTGAAACGC AGGCATCATG CATTTGCTGT TGTTGCCTAA GTGAAGATCT GCAGGTTGCT 
GCATTTGGAC AGAAGAATGG AACAGTAAAG GTAATAAACC TGCCAAATGG AAATGTTTTA 
AAATCCAGAA CTGGACACAC AGAAGCTGTG CAACATTGTC AGTTTACTTC TGATGGAAAC 
ACTCTTGTTT CAAGCTCAGA TGACTCTACT ATACAGGTGT GGAATTGGCA GTCAGAGGAA 
TGCATTTTTC TCAAAGGCCA CAATGAACCT GTCAAAAACT TTTCTCTTTT GAAACATTTA 
AGGCTTTTTT CATGGTCATT TGATGGGACA GTTAAGCTCT GGAACACAGT TAGTGGAGAA 
CTGGAAAGAG ATCTTACTTG CCATGAAGAT GCAATTCTTT CTTGTGCTAT TTCACCTGAT 
GGCACTATGT TTTCCACTGC ATCTGCTGAC AAGACTGCTA AGATATGGCG TTTTGAAAGT 
TTGTCAATTC TTTATAAACT AAATAGTCAC AAAAGCTGTG TGAGATGTTG TGCTTTCTCC 
AGTAATAATG AATTATTGGC CACAGGAGAT GACAATGGAG AAATAAGGAT ATGGCGTTTT 
GAAAGTTTGT CAATTCTTCA TAAACTAAAT AGTCACAAAA GCTGTGTGAG ATGTTGTGCT 
TTCTCCAGTA ATAATGAATT ATTGGCCACA GGAGATGACA ATGGAGAAAT AAGGTGGTGG 
AATGTGGATA CTGGCCAGCC TCTACAAACA TTCTACACAA ATGGAACCAA TCTGAAATCA 
TTGCATGTAT CCCCAGATTT CAGAGTATGT GTAACTGTAG ATAACCTTGG CATTCTTTAT 
GTGCTACAGA TTATGGAAAA ATCTTTTTAA