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A Potential off-target site may be displayed when the risk score is 0 if the PAM sequence is not present. Because the PAM is required for Cas9 targeting, these gRNA sequences are expected to provide high-specificity binding despite some similarity to off-target loci in the protospacer region.
Alignments | MISMATCHES | GENE |
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Left primer coordinates |
Left primer | Left primer Tm |
Right primer coordinates |
Right primer | Right primer Tm |
Product size |
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