Enterokinase Animal-Free, His, Bovine

Enterokinase Animal-Free, His, Bovine

GenScript Enterokinase Animal-Free, His, Bovine is the light chain of recombinant bovine enterokinase that is a single glycosylated polypeptide chain containing 200 amino acids and is produced under animal free process, which is more friendly and safer for drug or vaccine development, manufacture and other applications. This product is designed with a 6 × His tag for further removing from the digestion system by being captured by Ni2+ affinity chromatography.
Z03747
¥13,413.00

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Description

Enterokinase (EK) is a serine protease produced_x000D_ by cells of the duodenum, and is involved in mammalian digestion. It plays the role_x000D_ of turning trypsinogen to the active form of trypsin, and indirectly activates_x000D_ the pancreatic digestive enzymes. Enterokinase is a specific protease that_x000D_ cleaves after a lysine at its cleavage site: Asp-Asp-Asp-Asp-Lys. Enterokinase does_x000D_ not function if the cleavage site is followed by a proline. 
GenScript’s Enterokinase Animal-Free, His,_x000D_ Bovine is the light chain catalytic subunit of bovine enterokinase consisting_x000D_ of a single glycosylated polypeptide chain containing Ile801-His1035 (Accession_x000D_ # P98072). This recombinant protein is expressed in P. pastoris and purified to obtain high yields of the active_x000D_ enzyme. It is produced under an animal free process, and is suitable for drug and_x000D_ vaccine development, manufacture and other applications. This product is_x000D_ designed with a C-terminal 6 × His tag and can be removed after cleavage by Ni2+ affinity_x000D_ chromatography in resin (Cat. No. L00223) or magnetic_x000D_ beads (Cat. No. L00295).
Overview
Source Recombinant Enterokinase expressed in P. pastoris
Species Bovine
Tag His Tag

Properties
Molecular Weight Predicted MW: 27 kDa
Apparent MW: ~40 kDa, on SDS-PAGE under reducing conditions
Purity ≥ 95% as analyzed by SDS-PAGE
Endotoxin Level < 1.0 EU/μg of protein by gel clotting method
Biological Activity 5 U/μl
Unit definition: One unit is defined as the amount of enzyme sufficient to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95% completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, pH 7.4.
Formulation Supplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4.
Storage Buffer 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4.
Storage & Stability Upon receiving, the product remains stable for up to 6 months at -20 °C. This product is stable for up to 1 week at 37 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

For laboratory research or manufacture use only. Direct human use, including taking orally and injection and clinical use are forbidden.