Adenine base editor 8e
ABE8e

ABE8e

ABE8e

ABE8e is an innovative adenine base editor designed to enable precise genetic modifications by converting A-T base pairs to G-C base pairs without introducing double-stranded breaks or requiring donor DNA templates.​ ABE8e contains eight additional mutations in the TadA domain, augments the effectiveness and applicability of adenine base editing.
RC00010
¥40,000.00

Ask us a question
Overview
Description ABE8e is an innovative adenine base editor designed to enable precise genetic modifications by converting A-T base pairs to G-C base pairs without introducing double-stranded breaks or requiring donor DNA templates.​ ABE8e contains eight additional mutations in the TadA domain, augments the effectiveness and applicability of adenine base editing.

Applications
single guide RNA (sgRNA) dependent single-stranded DNA cleavage

Background
References David R Liu,et al.” Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity” Nat Biotechnol. 2020 Jul;38(7):883-891.

Quality Control Specifications
Purity ≥90% by SDS-PAGE, 49% by SEC-HPLC
Residual DNase Non-specific DNase activity
Endotoxin Level Control <10EU/mg

Properties
Expression System Recombinant Cas9-based fusion with double-ended NLS expressed by E.coli
Species S. pyogenes
Tag N-His tag
Molecular Weight 185.140 Kda
Storage & Stability ABE8e is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.

Examples
  • ABE8e
    • ABE8e

  • ABE8e
    • ABE8e