Prime editor 2
PE2

PE2

PE2

PE2 is a revolutionary genome editing technology that allows for precise genetic modifications with minimal unintended effects. It integrates a Cas9-nickase fusion protein with a reverse transcriptase and a prime-editing guide RNA (pegRNA), enabling flexible editing capabilities.PE 2 represents a cutting-edge development in genome editing technology, offering significant advantages over traditional methods in terms of accuracy and versatility.​
RC00004
¥80,000.00

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Overview
Description PE2 is a revolutionary genome editing technology that allows for precise genetic modifications with minimal unintended effects. It integrates a Cas9-nickase fusion protein with a reverse transcriptase and a prime-editing guide RNA (pegRNA), enabling flexible editing capabilities.PE 2 represents a cutting-edge development in genome editing technology, offering significant advantages over traditional methods in terms of accuracy and versatility.​

Applications
single guide RNA (sgRNA) dependent single-stranded DNA cleavage

Background
References David R Liu,et al. “Search-and-replace genome editing without double-strand breaks or donor DNA”. Nature. 2019 Dec;576(7785):149-157.

Quality Control Specifications
Purity ≥90% by SDS-PAGE, 83% by SEC-HPLC
Residual DNase Non-specific DNase activity
Endotoxin Level Control <10EU/mg

Properties
Expression System Recombinant Cas9-based fusion with double-ended NLS expressed by E.coli
Species S. pyogenes
Tag N-His tag
Molecular Weight 242.057KDa
Storage & Stability PE2 is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C.

Examples
  • PE2
    • PE2

  • PE2
    • PE2