GMP Anti-Human CD28 Antibody (F105), mAb, Mouse
GMP Anti-Human CD28 Antibody (F105), MAb, Mouse

Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) and GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) for 6 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

GMP Anti-Human CD28 Antibody (F105), MAb, Mouse

Accelerated stability test of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) at 25 ℃ showed that this product is stable at 25 ℃ for 30 days and maintained high purity and potency.

Freeze-thaw cycle stability test of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) showed that this product is stable for 5 times of freeze-thaw cycles and maintained high purity and potency.

GMP Anti-Human CD28 Antibody (F105), mAb, Mouse

This product is specific for Human CD28
GMPA02213
¥25,893.00

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Overview
Specificity This product is specific for Human CD28
Host Species Mouse
Immunogen Recombinant Human CD28
Conjugate Unconjugated

Applications
ex vivo cell culture process; Flow cytometry

Properties
Form Liquid
Storage Buffer Supplied in 10 mM Sodium Citrate, 150 mM NaCl, pH 6.5.
Concentration 5 mg/ml
Storage Instructions The product remains stable at -70 ± 10 °C. Avoid repeated freeze-thaw cycles. Please refer to the COA for specific expiry date.
Isotype Mouse IgG1κ
Clonality Monoclonal
Clone ID F105
Note GMP Anti-Human CD28 Antibody (F105), mAb, Mouse can be utilized as ancillary materials for Cell, Gene, and Tissue-Based Products and are manufactured under the standards listed below:
USP <1043>. Ancillary materials for Cell, Gene and Tissue-engineered products
ICH Q7 Good Manufacturing Practice Guide for Active Pharmaceutical Ingredients
NMPA: Technical guidelines for pharmaceutical research and evaluation of immune cell therapy products
Guideline and procedure specified in Chinese Pharmacopeia

Examples
  • GMP Anti-Human CD28 Antibody (F105), MAb, Mouse
    • GMP Anti-Human CD28 Antibody (F105), MAb, Mouse

    Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) and GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) for 6 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

  • GMP Anti-Human CD28 Antibody (F105), MAb, Mouse
    • GMP Anti-Human CD28 Antibody (F105), MAb, Mouse

    Accelerated stability test of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) at 25 ℃ showed that this product is stable at 25 ℃ for 30 days and maintained high purity and potency.

    Freeze-thaw cycle stability test of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) showed that this product is stable for 5 times of freeze-thaw cycles and maintained high purity and potency.


Quality Control Specifications
Appearance Clear, colorless liquid
pH 6.5 ± 0.5
Concentration 5.0 ± 0.5 mg/ml tested by A280 nm
Purity ≥ 95% as analyzed by SEC-HPLC
Sequence The coverage of the light chain and heavy chain sequence was 100% consistent with the theoretical amino acid sequence by mass spectrometry (MS)
Residual HCP ≤ 100.0 ng/mg by ELISA method
Residual HCD ≤ 10.0 pg/mg by quantitative PCR method
Residual Protein A ≤ 10.0 ng/mg by ELISA method
Endotoxin Level < 0.1 EU/mg by gel clotting method
Sterility Sterile
Reactivity Qualified

Background
Target Background CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. It plays an important role in cell proliferation and cytokine production and promotion of T-cell survival. It also enhances the production of IL-4 and IL-10 in T cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation.

For laboratory research and ex vivo cell isolation and culture applications. Direct human use, including taking orally and injection and clinical use are forbidden.