DNA ligase 4 Antibody, mAb, Rabbit
DNA Ligase 4 Antibody, MAb, Rabbit

Western blot analysis of DNA Ligase IV expression in Ramos cell lysate.

DNA ligase 4 Antibody, mAb, Rabbit

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
A03985
¥37,309.00

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Overview
Specificity This product is specific to DNA ligase 4
Host Species Rabbit
Immunogen A synthesized peptide derived from human DNA ligase 4
Species Reactivity Human
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • DNA Ligase 4 Antibody, MAb, Rabbit
    • DNA Ligase 4 Antibody, MAb, Rabbit

    Western blot analysis of DNA Ligase IV expression in Ramos cell lysate.


Background
Target Background Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
Synonyms DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; LIG4; LIG4S; Ligase IV;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.