HMGB2 Antibody, mAb, Rabbit
HMGB2 Antibody, MAb, Rabbit

Western blot analysis of HMGB2 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.

HMGB2 Antibody, mAb, Rabbit

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).
A03779
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Overview
Specificity This product is specific to HMGB2
Host Species Rabbit
Immunogen A synthesized peptide derived from human HMGB2
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • HMGB2 Antibody, MAb, Rabbit
    • HMGB2 Antibody, MAb, Rabbit

    Western blot analysis of HMGB2 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.


Background
Target Background DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).
Synonyms HMG 2; HMG B2; HMG-2; HMG2; HMGB2;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.