LC3B Antibody, mAb, Rabbit
LC3B Antibody, MAb, Rabbit

Western blot analysis of LC3B expression in (1) Human brain lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.

LC3B Antibody, mAb, Rabbit

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles.Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria.
A03371
¥37,309.00

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Overview
Specificity This product is specific to LC3B
Host Species Rabbit
Immunogen A synthesized peptide derived from human LC3B
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • LC3B Antibody, MAb, Rabbit
    • LC3B Antibody, MAb, Rabbit

    Western blot analysis of LC3B expression in (1) Human brain lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.


Background
Target Background Ubiquitin-like modifier involved in formation of autophagosomal vacuoles.Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria.
Synonyms ATG8F; LC3B; MAP1LC3B; MLP3B; MAP1LC3B;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.