p-SMAD2 (S250) Antibody, mAb, Rabbit
P-SMAD2 (S250) Antibody, MAb, Rabbit

Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate.

p-SMAD2 (S250) Antibody, mAb, Rabbit

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity).
A03239
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Overview
Specificity This product is specific to p-SMAD2 (S250)
Host Species Rabbit
Immunogen A synthesized peptide derived from human SMAD2 around the phosphorylation site of S250
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • P-SMAD2 (S250) Antibody, MAb, Rabbit
    • P-SMAD2 (S250) Antibody, MAb, Rabbit

    Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate.


Background
Target Background Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity).
Synonyms JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.