K27-linkage specific ubiquitin Antibody, mAb, Rabbit
K27-linkage Specific Ubiquitin Antibody, MAb, Rabbit

Western blot analysis of K27-linkage Specific Ubiquitin expression in HeLa cell lysate.

K27-linkage specific ubiquitin Antibody, mAb, Rabbit

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).
A02836
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Overview
Specificity This product is specific to K27-linkage specific ubiquitin
Host Species Rabbit
Immunogen A synthesized peptide derived from human K27-linkage specific ubiquitin
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • K27-linkage Specific Ubiquitin Antibody, MAb, Rabbit
    • K27-linkage Specific Ubiquitin Antibody, MAb, Rabbit

    Western blot analysis of K27-linkage Specific Ubiquitin expression in HeLa cell lysate.


Background
Target Background Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).
Synonyms FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.