SMAD5 Antibody, mAb, Rabbit
SMAD5 Antibody, MAb, Rabbit

Western blot analysis of Smad5 expression in HEK293 cell lysate.

SMAD5 Antibody, mAb, Rabbit

Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis.Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor.
A02611
¥37,309.00

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Overview
Specificity This product is specific to SMAD5
Host Species Rabbit
Immunogen A synthesized peptide derived from human SMAD5
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • SMAD5 Antibody, MAb, Rabbit
    • SMAD5 Antibody, MAb, Rabbit

    Western blot analysis of Smad5 expression in HEK293 cell lysate.


Background
Target Background Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis.Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor.
Synonyms Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.