p-eIF2A (S51) Antibody, mAb, Rabbit
P-eIF2A (S51) Antibody, MAb, Rabbit

Western blot analysis of Phospho-eIF2 alpha (Ser51) expression in (1)HeLa cell lysates treated with Calyculin A;(2) Untreated HeLa cell lysates.

p-eIF2A (S51) Antibody, mAb, Rabbit

Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC).
A02446
¥37,309.00

Ask us a question
Overview
Specificity This product is specific to p-eIF2A (S51)
Host Species Rabbit
Immunogen A synthesized peptide derived from human eIF2A around the phosphorylation site of S51
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • P-eIF2A (S51) Antibody, MAb, Rabbit
    • P-eIF2A (S51) Antibody, MAb, Rabbit

    Western blot analysis of Phospho-eIF2 alpha (Ser51) expression in (1)HeLa cell lysates treated with Calyculin A;(2) Untreated HeLa cell lysates.


Background
Target Background Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC).
Synonyms EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; EIF2A; EIF2S1; IF2A;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.