MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], mAb, Rabbit
MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], MAb, Rabbit

The detection of PBMCs with or without FMC63 CAR-T cells using MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], mAb, Rabbit (GenScript, A02330) demonstrated minimal nonspecific binding to PBMCs and specific recognition of FMC63-expressing CARs.

MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], mAb, Rabbit

This product is specific to FMC63 scFv and CARs derived from FMC63 scFv.
A02330
¥207,144.00

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Overview
Specificity This product is specific to FMC63 scFv and CARs derived from FMC63 scFv.
Host Species Rabbit
Immunogen FMC63 scFv
Conjugate iFluor 647

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Flow Cytometry 1:50

Properties
Form Liquid
Storage Buffer Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.03% ProClin300 and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Protein A affinity column
Isotype Rabbit IgG, κ
Clonality Monoclonal
Clone ID 24B2
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], MAb, Rabbit
    • MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], MAb, Rabbit

    The detection of PBMCs with or without FMC63 CAR-T cells using MonoRab™ Anti-FMC63 Antibody (24B2) [iFluor 647], mAb, Rabbit (GenScript, A02330) demonstrated minimal nonspecific binding to PBMCs and specific recognition of FMC63-expressing CARs.


Background
Target Background The FMC63 scFv (single-chain variable fragment) is a key component in many chimeric antigen receptor (CAR) designs, particularly in CAR-T cell therapies. Derived from a murine monoclonal antibody, FMC63 specifically targets human CD19, a cell surface protein highly expressed on B-cells, including malignant B-cells in B-cell leukemias and lymphomas. This scFv fragment is engineered by fusing the variable regions of the heavy and light chains of the antibody, preserving the antigen-binding capability while significantly reducing its size. This smaller, single-chain format enhances its integration into CAR constructs, enabling the recognition and destruction of CD19-positive cells by CAR-T cells. FMC63-based CARs are widely used in immunotherapy, particularly in FDA-approved treatments for B-cell malignancies, such as Kymriah® and Yescarta®.
Synonyms FMC63; FMC63 scFv; FMC63-scFv; FMC63-CD19; Anti-CD19 (FMC63) CAR; Anti-CD19 (FMC63)

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.