MonoRab™ GS Linker Antibody [PE], mAb, Rabbit
MonoRab™ GS Linker Antibody [PE], MAb, Rabbit

Figure 1. Flow cytometric analysis of Jurkat cells using anti-CD3 antibody containing a (G4S)3. linker and MonoRab™ GS Linker Antibody [PE], mAb, Rabbit (GenScript, A02314) (blue peak); Concentration-matched A02314 was used as isotype control (green).

MonoRab™ GS Linker Antibody [PE], mAb, Rabbit

This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2. linker, (G2S)4 linker, (G3S)3 linker, (G4S)2 linker and (G4S)3 linker, etc.
A02314
¥48,334.00

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Overview
Specificity This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker, (G4S)2 linker and (G4S)3 linker, etc.
Host Species Rabbit
Immunogen A synthetic peptide (GGGGSGGGGSGGGGS) coupled to BSA
Conjugate Phycoerythrin (PE)

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Flow Cytometry 1:50

Properties
Form Liquid
Storage Buffer Supplied in PBS (pH 7.2), containing gelatin and 0.03% ProClin300.
Storage Instructions Stored at 4°C and it should be protected from prolonged exposure to light. Do not freeze. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Protein A affinity column
Isotype Rabbit IgG, κ
Clonality Monoclonal
Clone ID 20H7
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • MonoRab™ GS Linker Antibody [PE], MAb, Rabbit
    • MonoRab™ GS Linker Antibody [PE], MAb, Rabbit

    Figure 1. Flow cytometric analysis of Jurkat cells using anti-CD3 antibody containing a (G4S)3. linker and MonoRab™ GS Linker Antibody [PE], mAb, Rabbit (GenScript, A02314) (blue peak); Concentration-matched A02314 was used as isotype control (green).


Background
Target Background A linker is an amino acid chain that connects two fused proteins, allowing for the appropriate separation of protein functional domains or the maintenance of necessary inter-domain interactions. The most commonly used flexible linker is the poly-Glycine-Serine (GS) linker. A very important application of the flexible GS linker is connecting the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs). Due to its flexible structure and appropriate linker sequence length, the (GGGGS)3 linker has been shown to allow for the correct orientation of the VH and VL domains, and it does not interfere with the folding of the protein domains in scFv construction[1,2].
Synonyms GS Linker;Glycine-serine linker; (G₄S)₃ Linker; poly-Glycine-Serine; (GGGGS)₃;
Reference [1] Huston,J,S,et al.Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.[J].Proceedings of the National Academy of Sciences, 1988, 85(16):5879-5883.
[2] Chen X , Zaro J ,Shen, Wei‐Chiang.Fusion Protein Linkers: Effects on Production, Bioactivity, and Pharmacokinetics[M].John Wiley & Sons, Inc. 2013.

For research use only. Not intended for human and animal therapeutic or diagnostic use.