Anti-Human CD3 Antibody (OKT3), mAb, Mouse
Anti-Human CD3 Antibody (OKT3), MAb, Mouse

Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human PBMC stained with Anti-Human CD3 Antibody (OKT3), mAb, Mouse (GenScript, Cat. No. A02199. Red) or with mouse IgG2a isotype control (Gray), followed by goat anti-mouse IgG PE-conjugated antibody. Data shown was gated on CD45 (+)/SSC Low lymphocytes.

Anti-Human CD3 Antibody (OKT3), mAb, Mouse

The product is specific for human CD3.
A02199
¥8,631.00

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Overview
Specificity The product is specific for human CD3.
Host Species Mouse
Immunogen Recombinant human CD3
Species Reactivity Human
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The suitability of this antibody for applications other than those listed below has not been determined. For flow cytometric staining, the following concentration range is recommended starting points for this product and is suggested that the reagent be titrated for optimal performance for each application.
Application Recommended Usage
Flow Cytometry ≤ 0.25 µg per million cells in 100 µl volume. It is strongly recommended that the reagent should be titrated for optimal performance for each application.

Properties
Form Liquid
Storage Buffer Supplied in 10 mM Sodium Citrate, 150 mM NaCl, 0.02 mg/ml Polysorbate 80, pH 5.0.
Concentration 1 mg/ml
Endotoxin Level < 0.5 EU/μg by gel clotting method
Storage Instructions The product remains stable up to 12 months at 4 °C from date of receipt. For longer time storage, store at -80 °C. Avoid repeated freeze-thaw cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a
Clonality Monoclonal
Note GenScript can customize this product per customer's request including conjugations, product size, buffer components, etc.

Examples
  • Anti-Human CD3 Antibody (OKT3), MAb, Mouse
    • Anti-Human CD3 Antibody (OKT3), MAb, Mouse

    Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Human PBMC stained with Anti-Human CD3 Antibody (OKT3), mAb, Mouse (GenScript, Cat. No. A02199. Red) or with mouse IgG2a isotype control (Gray), followed by goat anti-mouse IgG PE-conjugated antibody. Data shown was gated on CD45 (+)/SSC Low lymphocytes.


Background
Target Background CD3 is a member of the immunoglobulin superfamily, expressed on T cells. It is composed of four distinct chains (ε, γ, δ, ζ). CD3 antigen can form a complex with the T cell receptor (TCR) to generate an activation signal in T cells, which plays an important role in TCR signal transduction and both cytotoxic T cell ( CD8 + naïve T cells) and T helper cells (CD4+ naïve T cells) activation.
Synonyms CD3; cluster of differentiation 3

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.