一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » Primary Antibodies » AAV2 Antibody (12C10), mAb, Mouse
AAV2 Antibody (12C10), MAb, Mouse

Western Blot analysis of AAV2 Antibody (12C10), mAb, Mouse (GenScript, A02091) with different copy numbers of purified AAV2 samples.
Lane 1： 8×10^9 Copies of purified AAV2 loading amount
Lane 2： 4×10^9 Copies of purified AAV2 loading amount
Lane 3： 2×10^9 Copies of purified AAV2 loading amount
Lane 4： 8×10^8 Copies of purified AAV2 loading amount
Primary Antibody:
Lane 1-4 : AAV2 Antibody (12C10), mAb, Mouse (GenScript, A02091) 1 μg/ml
Secondary Antibody:
Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb 0.25 μg/ml

AAV2 Antibody (12C10), mAb, Mouse

The product is specific for AAV2 capsids VP1, VP2 and VP3.
A02091
¥41,429.00

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Overview
Specificity The product is specific for AAV2 capsids VP1, VP2 and VP3.
Host Species Mouse
Immunogen AAV2
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1 µg/ml 

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.2, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2c, k
Clonality Monoclonal
Clone ID 12C10
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • AAV2 Antibody (12C10), MAb, Mouse
    • AAV2 Antibody (12C10), MAb, Mouse

    Western Blot analysis of AAV2 Antibody (12C10), mAb, Mouse (GenScript, A02091) with different copy numbers of purified AAV2 samples.
    Lane 1： 8×10^9 Copies of purified AAV2 loading amount
    Lane 2： 4×10^9 Copies of purified AAV2 loading amount
    Lane 3： 2×10^9 Copies of purified AAV2 loading amount
    Lane 4： 8×10^8 Copies of purified AAV2 loading amount
    Primary Antibody:
    Lane 1-4 : AAV2 Antibody (12C10), mAb, Mouse (GenScript, A02091) 1 μg/ml
    Secondary Antibody:
    Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb 0.25 μg/ml


Background
Target Background Adeno-associated virus type 2 (AAV2) is a valuable vector for gene therapy. It is one member of the human parvovirus family which depends on co-infection with helper viruses such as adenovirus or herpes virus for efficient reproduction. AAV2 empty capsids are composed of three proteins, VP1, VP2 and VP3 with relative molecular masses of 87, 72 and 62 kDa, respectively.
Synonyms Adeno-associated virus serotype 2 Antibody (12C10), AAV2 Antibody

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.