MonoRab™ Anti-Atezolizumab Antibody (55D8), MAb, Rabbit

ELISA binding of MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8) (GenScript, A01984-40) with Atezolizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Atezolizumab, 1 µg/ml.
MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8) (GenScript, A01984-40) dilutions start from 1,000 ng/ml.
EC₅₀= 2.446 ng/ml.

Standard curve of Atezolizumab Sandwich ELISA. The Atezolizumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit (GenScript, A01984-40) and MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (Genscript, A01985-40) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabᵀᴹ Anti-Atezolizumab Antibody (43H11) (GenScript, A01985-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.The sensitivity of detecting Atezolizumab is up to 156 pg/ml.

MonoRabᵀᴹ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit (GenScript, A01984-40) blocks Atezolizumab binding with Human PD-L1 recombinant protein (Z03371, PD-L1 Fc Chimera, Human).
Coating antigen: Atezolizumab, 1 μg/ml.
PD-L1-Fc-biotin final concentration: 10 ng/ml.
Anti-Atezolizumab antibody dilutions start from 10 μg/ml.
IC₅₀= 0.39 μg/ml

MonoRab™ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit

The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A01985-40, MonoRab Anti-Atezolizumab Antibody (43H11), mAb, Rabbit.

Cat. No.	A01984
Size	40 μg Customized Size
Quantity
Price	¥36,423.00


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Overview

Specificity	The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A01985-40, MonoRab Anti-Atezolizumab Antibody (43H11), mAb, Rabbit.
Host Species	Rabbit
Immunogen	Atezolizumab
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Sandwich ELISA	Capture: 0.01-0.1 µg/ml
Competitive ELISA	10-50 µg/ml

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Rabbit IgG
Clonality	Monoclonal
Clone ID	55D8
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples

Background

Target Background

Atezolizumab is a fully humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of metastatic non-small cell lung cancer (NSCLC). It binds to the protein programmed cell death-ligand 1 (PD-L1) on several tumors. Atezolizumab has ability to block the binding of PD-L1 with PD-1 and CD80 receptors (B7-1Rs), which has a positive effect on improving an anti-tumor response.GenScript MonoRab™ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Atezolizumab.

Synonyms

Rabbit monoclonal to Tecentriq

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

MonoRab™ Anti-Atezolizumab Antibody (55D8), mAb, Rabbit

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