一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » MonoRab™ ウサギモノクローナル抗体製品 » MonoRab™ Anti-Adalimumab Antibody (134D5), mAb, Rabbit
MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit

ELISA binding of MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Adalimumab, 0.8 µg/ml.
Anti-Adalimumab antibody (GenScript, A01922-40) dilution start from 1,000 ng/ml.
EC₅₀= 7.295 ng/ml.

MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit

MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) blocks Adalimumab binding with TNF-α. Coating antigen: Adalimumab, 0.5 µg/ml.
Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) final concentration: 3.5 ng/ml.
TNF-α dilution start from 50 µg/ml.
IC₅₀= 353.1 ng/ml.

MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit

Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Adalimumab Antibody (4E12), mAb, Rabbit (GenScript, A01921-40) and MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit.
In this ELISA assay, MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per customer's request.
The sensitivity is < 2 ng/ml and the detection range is 0-33 ng/ml.

MonoRab™ Anti-Adalimumab Antibody (134D5), mAb, Rabbit

The product is specific for Adalimumab. This antibody blocks Adalimumab binding with TNF-α (Z01001, TNF-α, Human) in ELISA. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01921-40, MonoRab™ Anti-Adalimumab Antibody (4E12), mAb, Rabbit.
A01922
¥36,162.00

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Overview
Specificity The product is specific for Adalimumab. This antibody blocks Adalimumab binding with TNF-α (Z01001, TNF-α, Human) in ELISA. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01921-40, MonoRab™ Anti-Adalimumab Antibody (4E12), mAb, Rabbit.
Host Species Rabbit
Immunogen Adalimumab
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.1 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Purification Protein A affinity column
Isotype Rabbit IgG
Clonality Monoclonal
Clone ID 134D5
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit
  • MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit

    ELISA binding of MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
    Coating antigen: Adalimumab, 0.8 µg/ml.
    Anti-Adalimumab antibody (GenScript, A01922-40) dilution start from 1,000 ng/ml.
    EC₅₀= 7.295 ng/ml.

  • MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit
  • MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit

    MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) blocks Adalimumab binding with TNF-α. Coating antigen: Adalimumab, 0.5 µg/ml.
    Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) final concentration: 3.5 ng/ml.
    TNF-α dilution start from 50 µg/ml.
    IC₅₀= 353.1 ng/ml.

  • MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit
  • MonoRab™ Anti-Adalimumab Antibody (134D5), MAb, Rabbit

    Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Adalimumab Antibody (4E12), mAb, Rabbit (GenScript, A01921-40) and MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit.
    In this ELISA assay, MonoRabᵀᴹ Anti-Adalimumab Antibody (134D5), mAb, Rabbit (GenScript, A01922-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per customer's request.
    The sensitivity is < 2 ng/ml and the detection range is 0-33 ng/ml.


Background
Target Background Adalimumab (Humira) is a humanized monoclonal antibody that’s approved by the U.S. Food and Drug Administration for the treatment of patients with rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, ulcerative colitis, chronic psoriasis, hidradenitis suppurativa, and juvenile idiopathic arthritis. Adalimumab binds to the TNF-α (tumor necrosis factor-alpha), and blocks its interaction with TNF-α receptors, reducing the inflammatory response of autoimmune diseases.
Synonyms Rabbit monoclonal to Humira

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.