Western blot analysis of tyrosine phosphorylation status in different cells with THE^TM Phosphotyrosine Antibody (E10)^plus (A01819, 1 µg/ml)
1. EGF-stimulated A431 cell lysates (50 µg).
2. Untreated A431 cell lysates (50 µg).
3. Insulin-stimulated HEK293 cell lysates (50 µg).
4. Untreated HEK293 cell lysates (50 µg).
The signal was developed with IRDye^TM800 Conjugated affinity Purified Goat Anti-Mouse IgG.

Western blot comparison of a panel of phosphotyrosine antibodies with EGF-stimulated A431 cell lysates. THE^TM Phosphotyrosine Antibody (E10)^plus detects the most bands representative of various phosphotyrosine residues.
1. Phosphotyrosine Antibody (5E10), mAb, Mouse (A01817, 1 µg/ml)
2. Phosphotyrosine Antibody(18E10), mAb, Mouse (A01818, 1 µg/ml)
3. THE^TM Phosphotyrosine Antibody (E10)^plus (A01819, 1 µg/ml)
4. Anti-Phosphotyrosine, 4G10 ® Platinum (Millipore, 05-1050, 1:1000)
The signal was developed with IRDye^TM800 Conjugated affinity Purified Goat Anti-Mouse IgG.

Immunocytochemistry/Immunofluorescence analysis of EGF-stimulated A431 cell and untreated A431 cell with THE^TM Phosphotyrosine Antibody (E10)^plus (A01819, 4 µg/ml).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Flow cytometric analysis of EGF-stimulated A431 cell and untreated A431 cell with THE^TM Phosphotyrosine Antibody (E10)^plus (A01819, 4 µg/ml) (red and black respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

ELISA analysis of various phosphotyrosine peptides and corresponding non-phosphorylated peptides with THE^TM Phosphotyrosine Antibody (E10)^plus (A01819, 0.1 µg/ml). Results demonstrate the antibody has excellent recognition of different phosphotyrosine peptides and no cross-reactivity with non-phosphorylated peptides.