Figure 1. Double-antigen sandwich ELISA analysis of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795). PEG antibody was captured by PEG40K-OVA and detected by PEG20K-Biotin and Streptavidin-HRP conjugate. The test result shows that GenScript’s PEG Antibody is suitable for Bridging ELISA application.

Figure 2. Immunohistochemistry analysis of mouse kidney tissue (Paraffin embedded) using THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; 10 µg/mL) after mice were injected with BSA-PEG as an immunogen. The test results show that GenScript’s PEG Antibody is suitable for IHC application.

Figure 3. In-Cell ELISA analysis of PEGylated drug-HepG2 interaction using THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; 1 µg/mL) after HepG2 cells were treated with PEGylated drug (PEGASYS®, Peginterferon alfa-2a) at different concentrations. The test results show that GenScript’s PEG Antibody is suitable for the analysis of PEGylated drug-cell interaction.

Figure 4. Sensitivity comparison of competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4 ) with THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) by Western Blot.

A,C: PEGylated drug (PEGASYS®, Peginterferon alfa-2a)
B,D: Interferon Alfa 2A protein

In both panels A and B, the primary antibody of was Mouse Anti-PEG mAb (Company A, Clone AGP4) used at 0.2 µg/mL concentration;
In both panels C and D, the primary antibody of was THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) used at 0.2 µg/mL concentration;

The test results show that GenScript’s PEG Antibody has better sensitivity and specificity than the competitor’s product.

Figure 5. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red), and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; black) by indirect ELISA, with plate coated with PEG40K-OVA. The test results show that GenScript’s PEG Antibody had better reactivity to PEG40K-OVA than the competitor’s product.

Figure 6. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; blue), and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; red) by indirect ELISA. The test results show that GenScript’s PEG Antibody could detect PEG with different molecular weights and has better reactivity to PEG40K, PEG20K, PEG5K and PEG12 (Pierce, MES(PEG)12) than the competitor’s product.

Figure 7. Sensitivity comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red) and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; yellow) by indirect ELISA. The test results show that GenScript’s PEG Antibody had better reactivity to PEG20K than the competitor’s product.

Figure 8. The half maximal inhibitory concentration (IC50) comparison of THE™ PEG Antibody, mAb, Mouse (GenScript, A01795; red) and competitor’s Mouse Anti-PEG mAb (Company A, Clone AGP4; black) in competitive ELISA. The ELISA plate was coated with PEG40K-OVA, and unconjugated PEG40K was used for determining the IC50 of anti-PEG mAb. The test results showed that GenScript’s PEG Antibody had lower IC50, therefore, better specificity and higher affinity to PEG were validated.