Flow cytometric analysis of non-transfected CHO cells (a) and Avi fusion protein transfeced CHO cells (b) using Avi Antibody, mAb, Mouse (GenScript, A01738). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Immunocytochemistry/Immunofluorescence analysis of Avi tagged protein transfeced CHO cells using Avi Tag Antibody, mAb, Mouse (GenScript, A01738) and Mouse IgG Control (GenScript, A01007).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Western blot analysis of C-terminal Avi tagged fusion protein using Avi Tag Antibody, mAb, Mouse (GenScript, A01738, 0.2 µg/ml).
The signal was developed with IRDye^TM 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

ELISA analysis of N-terminal and C-terminal Avi tagged fusion proteins using Avi Tag Antibody, mAb, Mouse (GenScript, A01738).

Flow cytometric analysis of non-transfected or AVI fusion gene transfected CHO cells using
AVI Tag Antibody, mAb, Mouse (GenScript, A01738) (black and purple respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Avi Tag Antibody, mAb, Mouse

GenScript Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.

Cat. No.	A01738
Size	40 μg Customized Size
Quantity
Price	¥17,607.00


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Overview

Specificity	GenScript Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.
Host Species	Mouse
Immunogen	Avi peptide conjugated to KLH
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	0.2-1 µg/ml
Flow Cytometry	1-3 µg for 1 x 10⁶ cells
Immunocytochemistry/Immunofluorescence (ICC/IF)	1-3 µg/ml
ELISA	0.02-0.05 µg/ml

Properties

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2a,κ
Clonality	Monoclonal
Clone ID	1D11D10

Examples

ELISA analysis of N-terminal and C-terminal Avi tagged fusion proteins using Avi Tag Antibody, mAb, Mouse (GenScript, A01738).

Background

Target Background	Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.
Synonyms	BAP Tag antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Avi Tag Antibody, mAb, Mouse

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