一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse
THE™ NWSHPQFEK Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of N-terminal and C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse (GenScript, A01737, 1 µg/ml)
The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensor THETM HRP Substrate Kit (GenScript, L00221)

THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse

THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
A01737
¥62,143.00

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Overview
Specificity THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Host Species Mouse
Immunogen NWSHPQFEK peptide conjugated to KLH
Conjugate Biotin

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 0.2-1 µg/ml
ELISA 0.001-0.002 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 5A9F9

Examples
  • THE™ NWSHPQFEK Tag Antibody [Biotin], MAb, Mouse
    • THE™ NWSHPQFEK Tag Antibody [Biotin], MAb, Mouse

    Western blot analysis of N-terminal and C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse (GenScript, A01737, 1 µg/ml)
    The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensor THETM HRP Substrate Kit (GenScript, L00221)


Background
Target Background Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.GenScript THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
Synonyms THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.