Cross-reactivity of PSA monoclonal antibodies by Indirect ELISA:
General conditions:
1. Microplate was coated with total PSA, free PSA, ACT-PSA respectively, followed by 3 washing cycles.
2. Incubation with mouse anti-PSA followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.
Antibody pairs analysis of PSA monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was coated with a capture antibody against PSA, followed by 3 washing cycles.
2. Incubation with free PSA or total PSA followed by 3 washing cycles.
3. Incubation with peroxidase conjugated detection antibody against PSA, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.
PSA Antibody (8A9B8), mAb, Mouse
A01664 | |
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¥33,661.00 | |
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