一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » THE Elite抗体 » THE™ cAMP/cGMP mAb » THE™ cAMP Antibody, mAb, Mouse
THE™ CAMP Antibody, MAb, Mouse

cAMP Competitive ELISA
1.ELISA plate was coated with goat anti mouse IgG antibody
2.Anti-cAMP monoclonal antibody (Cat. No. A01509) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells.
3.After a period of incubation, cAMP-HRP conjugate (Cat.No.M01059) was added followed by proper period of incubation.
4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

THE™ cAMP Antibody, mAb, Mouse

The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding.
A01509
¥62,181.00

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Overview
Specificity The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding.
Host Species Mouse
Immunogen 3', 5'-cyclic AMP-8-KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.04-0.1 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 4H2B6
Note GenScript can offer this product according to your requirement, including product size, buffer components,etc.

Examples
  • THE™ CAMP Antibody, MAb, Mouse
    • THE™ CAMP Antibody, MAb, Mouse

    cAMP Competitive ELISA
    1.ELISA plate was coated with goat anti mouse IgG antibody
    2.Anti-cAMP monoclonal antibody (Cat. No. A01509) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells.
    3.After a period of incubation, cAMP-HRP conjugate (Cat.No.M01059) was added followed by proper period of incubation.
    4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
    5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.


Background
Target Background Adenosine 3, 5-cyclic monophosphate (cyclic AMP; cAMP) is one of the most important intracellular secondary messengers for transduction events. cAMP is also involved in regulating neuronal, glandular, cardiovascular, immune, and other functions and actions. A number of hormones, such as ACTH, TSH, FSH, and LH, are known to activate cAMP through the action of the enzyme adenylate cyclase, which converts ATP to cAMP. There remains considerable interest in the measurement of intracellular cAMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states. Due to the involvement of cAMP in amplifying the response of ligand binding, the second messenger cAMP has been largely employed to monitor the activation of GPCR to facilitate therapeutic drug discovery.GenScript THE™ cAMP Antibody, mAb, Mouse is developed using 3, 5-cyclic AMP-8-KLH as immunogen.
Synonyms THE™ Anti-cAMP Monoclonal Antibody (Mouse);

Limited Use License and Use Restrictions
Limited Use License and Use Restrictions This Product is provided under a limited, non-exclusive, non-transferable, and non-sublicensable end-user license strictly limited to the permitted uses set forth in GenScript’s official documentation, quotations, or written agreements. Any use beyond such scope requires GenScript’s prior written authorization or license.
Except as expressly authorized in writing by GenScript, Customer shall not, and shall not permit, procure, enable, or assist any third party to: (a) commercialize or otherwise exploit the Product, including by reselling, distributing, transferring, sublicensing, or otherwise making the Product (including any product incorporating the Product) available to any third party; (b) copy, reproduce, clone, reverse clone, sequence, analyze, characterize, deconstruct, reconstruct, reverse engineer, or otherwise attempt to discover the composition, sequence, structure, binding characteristics, epitope recognition characteristics, or other proprietary features of the Product; (c) modify, derivatize, humanize, optimize, mutate, design around, or generate any variants, derivatives, fragments, substitutes, or functional equivalents of the Product; or (d) use the Product, any information derived therefrom, or any computational, AI-enabled, or sequence-based design method to develop, manufacture, offer for sale, sell, or commercialize any product or service competitive with the Product or GenScript’s products or services.
All intellectual property rights and all rights not expressly granted herein are reserved by GenScript. No rights are granted by implication, estoppel, or otherwise.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.