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THE™ HA Tag Antibody, MAb, Mouse

Figure 1. The affinity of THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) for a C-terminal HA tag was analyzed in an immunocytochemistry/immunofluorescence analysis of C-terminal HA tag protein-transfected HEK293 cells using THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244). The primary antibody was THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) used at 1 μg/mL concentration. The secondary antibody was Fluorescein Conjugated Anti-mouse IgG used at 2 μg/mL concentration.

THE™ HA Tag Antibody, MAb, Mouse

Figure 2. The affinity of THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) for an N-terminal HA tag was analyzed in an immunocytochemistry/immunofluorescence analysis of N-terminal HA tag protein-transfected HEK 293 cells using THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244). The primary antibody was THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) used at 1 μg/mL concentration. The secondary antibody was Fluorescein Conjugated Anti-Mouse IgG used at 2 μg/mL concentration.

THE™ HA Tag Antibody, MAb, Mouse

Figure 3. The detection of HA tag by THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) was analyzed by flow cytometric analysis of non-transfected or HA gene transfected CHO cells (black and pink, respectively) using
THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE™ HA Tag Antibody, MAb, Mouse

Figure 4. Comparative HA-tag detection was analyzed for THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244; panel A), and Competitors’ product (panel B) using Western blotting.
In both panels A and B:
Lanes 1-3: 2 μL, 0.4 μL, 0.08 μL N-terminal HA-GST fusion protein in E. coli cell lysate
Lanes 4-6: 2 μL, 0.4 μL, 0.08 μL C-terminal HA-GST fusion protein in E. coli cell lysate
Lanes 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
Lane 10: E. coli cell lysate

The primary antibody in panel A was THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) used at 1 μg/mL concentration. The primary antibody in panel B was Mouse Anti-HA Monoclonal Antibody (Competitor’s product) at 1 μg/mL concentration. In both cases, the secondary antibody was IRDye800 Conjugated Goat Anti-Mouse IgG.

THE™ HA Tag Antibody, mAb, Mouse

THE™_x000D_ HA Tag Antibody, mAb, Mouse (A01244) recognizes HA tags localized at the C-terminal,_x000D_ N-terminal, and internal region of HA tagged-fusion proteins.
A01244
¥50,923.00

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Overview
Specificity THE™ HA Tag Antibody, mAb, Mouse (A01244) recognizes HA tags localized at the C-terminal, N-terminal, and internal region of HA tagged-fusion proteins.
Host Species Mouse
Immunogen A KLH coupled synthetic peptide (YPYDVPDYA)
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Immunoprecipitation (IP) 1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF) 1 µg/ml
Flow Cytometry 1 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1
Clonality Monoclonal
Clone ID 5E11D8
Note GenScript can offer this product per customer's request including product size, buffer components, etc. 

Examples
  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    Figure 1. The affinity of THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) for a C-terminal HA tag was analyzed in an immunocytochemistry/immunofluorescence analysis of C-terminal HA tag protein-transfected HEK293 cells using THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244). The primary antibody was THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) used at 1 μg/mL concentration. The secondary antibody was Fluorescein Conjugated Anti-mouse IgG used at 2 μg/mL concentration.

  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    Figure 2. The affinity of THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) for an N-terminal HA tag was analyzed in an immunocytochemistry/immunofluorescence analysis of N-terminal HA tag protein-transfected HEK 293 cells using THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244). The primary antibody was THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) used at 1 μg/mL concentration. The secondary antibody was Fluorescein Conjugated Anti-Mouse IgG used at 2 μg/mL concentration.

  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    Figure 3. The detection of HA tag by THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) was analyzed by flow cytometric analysis of non-transfected or HA gene transfected CHO cells (black and pink, respectively) using
    THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    Figure 4. Comparative HA-tag detection was analyzed for THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244; panel A), and Competitors’ product (panel B) using Western blotting.
    In both panels A and B:
    Lanes 1-3: 2 μL, 0.4 μL, 0.08 μL N-terminal HA-GST fusion protein in E. coli cell lysate
    Lanes 4-6: 2 μL, 0.4 μL, 0.08 μL C-terminal HA-GST fusion protein in E. coli cell lysate
    Lanes 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
    Lane 10: E. coli cell lysate

    The primary antibody in panel A was THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) used at 1 μg/mL concentration. The primary antibody in panel B was Mouse Anti-HA Monoclonal Antibody (Competitor’s product) at 1 μg/mL concentration. In both cases, the secondary antibody was IRDye800 Conjugated Goat Anti-Mouse IgG.


Background
Target Background GenScript’s THE™ HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody for HA-tagged fusion protein detection. It belongs to our THE™ elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the bio-distribution of recombinant proteins. It has been extensively used as a general epitope tag in expression vectors. THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106.
Synonyms Mouse Anti-HA-Tag Monoclonal Antibody

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.