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Enterokinase Antibody, MAb, Mouse

Western blot analysis of Enterokinase protein using Enterokinase Antibody, mAb, Mouse (GenScript, A00889, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 38 kD
Observed Size: 38 kD

Enterokinase Antibody, mAb, Mouse

Recombinant Enterokinase protein
A00889
¥19,506.00

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Overview
Specificity Recombinant Enterokinase protein
Host Species Mouse
Immunogen Purified recombinant light chain of bovine EK protein
Species Reactivity Bovine and porcine.Reactivity to other species is not tested yet.
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration.We have not yet determined the suitability of this antibody for other applications.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1
Clonality Monoclonal
Clone ID 5E3C8

Examples
  • Enterokinase Antibody, MAb, Mouse
    • Enterokinase Antibody, MAb, Mouse

    Western blot analysis of Enterokinase protein using Enterokinase Antibody, mAb, Mouse (GenScript, A00889, 1 µg/ml)
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 38 kD
    Observed Size: 38 kD


Background
Target Background Enterokinase is an intestinal enzyme responsible for initiating activation of pancreatic proteolytic proenzymes. Mouse Anti-Enterokinase Monoclonal Antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant EK protein (bovine EK, aa848-1035, light chain, 93% homologous with human EK).Mouse Anti-Enterokinase Monoclonal Antibody recognizes EK in Western blots and ELISAs.
Synonyms Mouse Anti-Enterokinase mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.