一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » NWSHPQFEK Antibody [HRP], pAb, Rabbit
NWSHPQFEK Antibody [HRP], PAb, Rabbit

Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody [HRP], pAb, Rabbit (GenScript, A00875, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 60 kD
Observed Size: 60 kD

NWSHPQFEK Antibody [HRP], pAb, Rabbit

This Antibody recognizes Strep-tag II tagged fusion proteins.
A00875
¥17,607.00

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Overview
Specificity This Antibody recognizes Strep-tag II tagged fusion proteins.
Host Species Rabbit
Immunogen Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH.
Conjugate Horseradish peroxidase (HRP)

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.5-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Examples
  • NWSHPQFEK Antibody [HRP], PAb, Rabbit
    • NWSHPQFEK Antibody [HRP], PAb, Rabbit

    Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody [HRP], pAb, Rabbit (GenScript, A00875, 1 µg/ml)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 60 kD
    Observed Size: 60 kD


Background
Target Background Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered.The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase.The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK.
Synonyms Rabbit anti NWSHPQFEK [HRP] pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.