一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » THE™ c-Myc Antibody [Biotin], mAb, Mouse
THE™ C-Myc Antibody [Biotin], MAb, Mouse

Lane 1-3: 200 ng, 50 ng, 10 ng Multiple Tag Cell Lysate (GenScript, M0100)
Lane 4: Negative Control
Detect antibody: 0.1 µg/ml THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) (GenScript, A00864)
Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

THE™ c-Myc Antibody [Biotin], mAb, Mouse

GenScript THE™ c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells.It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.
A00864
¥59,554.00

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Overview
Specificity GenScript THE™ c-Myc Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells.It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.
Host Species Mouse
Immunogen A synthetic peptide EQKLISEEDL coupled to KLH
Conjugate Biotin

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 2G8D5

Examples
  • THE™ C-Myc Antibody [Biotin], MAb, Mouse
    • THE™ C-Myc Antibody [Biotin], MAb, Mouse

    Lane 1-3: 200 ng, 50 ng, 10 ng Multiple Tag Cell Lysate (GenScript, M0100)
    Lane 4: Negative Control
    Detect antibody: 0.1 µg/ml THETM Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) (GenScript, A00864)
    Secondary antibody: Streptavidin-HRP (GenScript, M00091, 1:10,000)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)


Background
Target Background GenScript THE™ c-Myc Antibody [Biotin], mAb, Mouse is the biotin conjugated THE™ Anti-c-Myc Monoclonal Antibody (Mouse) (A00704) purified from mice ascites by protein A affinity column.This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Synonyms Mouse Anti-c-Myc [Biotin] mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.