Lane 1-3: DDDDK tag fusion protein in E. coli lysates
Lane 4: E. coli lysates Negative Control

The result was developed with One-Step Western^TM Complete Kit (Rabbit) (GenScript, L00204C)

Lane 1-3: DDDDK tag fusion protein in E. coli lysates
Lane 4: E. coli lysates Negative Control
Primary Antibody: 1 µg/ml Rabbit Anti-DDDDK-tag Polyclonal Antibody (GenScript, A00676)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

DDDDK-tag Antibody, pAb, Rabbit

This Antibody recognizes the C-terminal enterokinase cleavage site and specifically binds to C-terminal xxxDDDDK tagged fusion proteins, such as Xpress tag or Flag tag.

Cat. No.	A00676
Size	100 μg : Quote Buy in bulk
Quantity
Price	¥35,387.00


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Overview

Specificity	This Antibody recognizes the C-terminal enterokinase cleavage site and specifically binds to C-terminal xxxDDDDK tagged fusion proteins, such as Xpress tag or Flag tag.
Host Species	Rabbit
Immunogen	A synthetic epitope peptide xxxDDDDK conjugated to KLH.This sequence represents the enterokinase cleavage site (ECS).
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Western Blot	1:100-1:1,000

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable

Examples

Lane 1-3: DDDDK tag fusion protein in E. coli lysates
Lane 4: E. coli lysates Negative Control

The result was developed with One-Step Western^TM Complete Kit (Rabbit) (GenScript, L00204C)

Background

Target Background

Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.The GenScript Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.

Synonyms

Rabbit Anti-DDDDK-tag pAb;Anti-DDDDK-tag pAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

DDDDK-tag Antibody, pAb, Rabbit

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