CBP-tag Antibody, pAb, Rabbit

Overview

Specificity	GenScript CBP-tag Antibody recognizes CBP tagged fusion proteins.
Host Species	Rabbit
Immunogen	A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated to KLH
Species Reactivity	Mouse, rat, bovine and rabbit
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Western Blot	1:100-1:1,000

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable

Examples

Lane 1-2: CBP tag fusion protein expressed in E. coli Cell Lysate
Antibody: 1 µg/ml Rabbit Anti-CBP-tag Polyclonal Antibody (GenScript, A00635)
The result was developed with One-Step Western^TM Basic Kit (GenScript, L00204C)

Western blot analysis of CBP tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-CBP-tag Polyclonal Antibody (GenScript, A00635, 1 µg/ml) The signal was developed with IRDy^TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot analysis of CBP-tagged protein from the transfected CHO cell lysates and its immuno-precipitates.
Lane 1: CBP-tagged protein from the transfected CHO cell lysates as input control.
Lane 2: Immuno-precipitates of CBP-tagged protein from the transfected CHO cell lysates with CBP Tag Antibody, pAb, Rabbit (GenScript, A00635).
Lane 3: Immuno-precipitates of the CBP-tagged protein from the transfected CHO cell lysates with Rabbit IgG Control (Whole Molecule), Purified (GenScript, A01008).

Background

Target Background

CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.GenScript Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH.CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.The antibody is suitable for detecting CBP fusion proteins.

Synonyms

Rabbit Anti-CBP-tag pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Cat. No.	A00635
Size	40 μg : Quote Buy in bulk
Quantity
Price	¥17,607.00


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CBP-tag Antibody, pAb, Rabbit

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