Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step Western^TM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml) The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

V5-tag Antibody, pAb, Rabbit

GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.

Cat. No.	A00623
Size	40 μg : Quote Buy in bulk
Quantity
Price	¥17,607.00


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Overview

Specificity	GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Host Species	Rabbit
Immunogen	V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Western Blot	1:100-1:1,000

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable

Examples

Background

Target Background	The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Synonyms	Rabbit Anti-V5-tag pAb;Anti-V5-tag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

V5-tag Antibody, pAb, Rabbit

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