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Streptavidin Antibody, pAb, Rabbit

GenScript Streptavidin Antibody is specific to streptavidin.
A00621
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Overview
Specificity GenScript Streptavidin Antibody is specific to streptavidin.
Host Species Rabbit
Immunogen Purified recombinant streptavidin
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.5-1 µg/ml
Dot blot 0.5-1 µg/ml
Western Blot 0.5-1 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized in PBS, pH7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A/G affinity column
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Background
Target Background Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ≈10-14 mol/L.The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.GenScript Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.
Synonyms Rabbit Anti-Streptavidin pAb;Anti Streptavidin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.