一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » THE™ His Tag Antibody [HRP], mAb, Mouse
THE™ His Tag Antibody [HRP], MAb, Mouse

Figure 1. A recombinant His-tagged protein was detected by Western blotting using THE™ His Tag Antibody [HRP], mAb, Mouse (A00612) at 1 µg/mL concentration.
The signal was developed with LumiSensor™ HRP Substrate Kit (GenScript, L00221, discontinued).

THE™ His Tag Antibody [HRP], mAb, Mouse

THE™_x000D_ His Tag Antibody [HRP], mAb, Mouse recognizes His tags localized at C-terminal,_x000D_ N-terminal, and internal region of fusion proteins. 
A00612
¥73,364.00

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Overview
Specificity THE™ His Tag Antibody [HRP], mAb, Mouse recognizes His tags localized at C-terminal, N-terminal, and internal region of fusion proteins. 
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH
Conjugate Horseradish peroxidase (HRP)

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. 
Application Recommended Usage
ELISA 0.05-0.5 µg/ml
Western Blot 0.1-1.0 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20°C from date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Upon receiving, spin the vial down to place the lyophilized antibody on the bottom of the vial. Reconstitute the antibody as mentioned above. Prepare single use aliquots (no less than 10µl) in amber vials and freeze it immediately at -20oC. The frozen aliquots should be thawed once with any remainder kept at 4oC.
Purification Purified by Protein A affinity chromatograph.
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 6G2A9

Examples
  • THE™ His Tag Antibody [HRP], MAb, Mouse
    • THE™ His Tag Antibody [HRP], MAb, Mouse

    Figure 1. A recombinant His-tagged protein was detected by Western blotting using THE™ His Tag Antibody [HRP], mAb, Mouse (A00612) at 1 µg/mL concentration.
    The signal was developed with LumiSensor™ HRP Substrate Kit (GenScript, L00221, discontinued).


Background
Target Background GenScript’s THE™ His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THE™ Anti-His Monoclonal Antibody (Mouse) (A00186) purified by protein A affinity chromatograph. This product is suitable for detecting the expression level of His-tagged proteins various immunoassays.
Synonyms His-tag Antibody [HRP],mAb, Mouse

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.