一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » VSV-G-tag Antibody, pAb, Rabbit
VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 0.1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from VSV-G fusion protein lysates using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).
Lane 1: Input control material for VSV-G fusion protein lysates
Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008)
Lane 3: Immunprecipitation with Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).

VSV-G-tag Antibody, PAb, Rabbit

Western blot analysis of VSV-G tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

VSV-G-tag Antibody, pAb, Rabbit

This Antibody recognizes VSV-G tagged fusion proteins.
A00199
¥17,607.00

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Overview
Specificity This Antibody recognizes VSV-G tagged fusion proteins.
Host Species Rabbit
Immunogen VSV-G epitope tag peptide YTDIEMNRLGK conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Immunoprecipitation (IP) 2-10 µg/mg of lysate

Properties
Form Lyophilized
Storage Buffer Lyophilized in PBS, pH7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 1 mg/ml.
Storage Instructions The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Examples
  • VSV-G-tag Antibody, PAb, Rabbit
  • VSV-G-tag Antibody, PAb, Rabbit

    Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 0.1 µg/ml)
    The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 52 kD
    Observed Size: 52 kD

  • VSV-G-tag Antibody, PAb, Rabbit
  • VSV-G-tag Antibody, PAb, Rabbit

    Western blot analysis of VSV-G-tagged fusion protein using VSV-G-tag Antibody, pAb, Rabbit (GenScript, A00199, 1 µg/ml)
    The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
    Predicted Size: 52 kD
    Observed Size: 52 kD

  • VSV-G-tag Antibody, PAb, Rabbit
  • VSV-G-tag Antibody, PAb, Rabbit

    Western blot analysis of immunoprecipitates from VSV-G fusion protein lysates using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).
    Lane 1: Input control material for VSV-G fusion protein lysates
    Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008)
    Lane 3: Immunprecipitation with Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199).

  • VSV-G-tag Antibody, PAb, Rabbit
  • VSV-G-tag Antibody, PAb, Rabbit

    Western blot analysis of VSV-G tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-VSV-G-tag Polyclonal Antibody (GenScript, A00199, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Background
Target Background Rabbit Anti-VSV-G-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.It is purified by immunoaffinity chromatography.
Synonyms Anti-VSV-G-tag;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.