一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » エピトープタグ抗体ファミリー » THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK_x000D_ tagged fusion proteins.
A00187
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Overview
Specificity THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. 
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Immunoprecipitation (IP) 1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF) 1 µg/ml
Flow Cytometry 1 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PB, pH 6.5, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions The product remains stable for 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2b,κ
Clonality Monoclonal
Clone ID 5A8E5
Note GenScript can offer this product per customer's request including product size, buffer components, etc. 

Background
Target Background The DYKDDDDK peptide is a small epitope tag which is widely used in expression vectors. The tag does not appear to interfere with the bioactivity or bio-distribution of the tagged recombinant proteins. THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins
Synonyms Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.