GenCRISPR™ ゲノム編集関連製品 » GenCRISPR™ ヌクレアーゼ-CRISPR遺伝子編集 » GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free
GenCRISPR™ Ultra ESpCas9-N-NLS Research, Tag-free

A 20 μl reaction in 1 × Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free

GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization signal (N-NLS). GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Z03692
¥138,657.00

Ask us a question
Description

The GenCRISPR™_x000D_ Ultra eSpCas9 product line provides customers with a selection of research use,_x000D_ basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with_x000D_ the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex_x000D_ to perform gene editing has been shown to reduce the challenges encountered_x000D_ with other CRISPR gene editing techniques such as viral and plasmid delivery._x000D_ Challenges include off-target effects, cell viability and transcription/translational_x000D_ challenges.

_x000D_ _x000D_ _x000D_ _x000D_ GenCRISPR™ Ultra eSpCas9-N-NLS_x000D_ Research, tag-free is a mutant form of Cas9_x000D_ nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with an N-terminal_x000D_ nuclear localization signal (N-NLS). GenCRISPR™ Ultra eSpCas9-N-NLS Research,_x000D_ tag-free delivers higher fidelity_x000D_ and less off-target activity than wild-type SpCas9 nuclease.
Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Applications
gRNA-dependent double-stranded DNA cleavage

Properties
Expression System Recombinant eSpCas9 with an N-terminal NLS expressed by E.coli
Species S. pyogenes
Tag Tag-free
Molecular Weight ~160 kDa
Concentration 10 mg/ml
Active temperature This eSpCas9 is active at 37°C.
Formulation Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C. 
Storage & Stability This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Key Features High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Tag-free: No extra tag amino acid
DNA-free: No external DNA added to the system.
Quality Control Specifications
AssaySpecifications
AppearanceClear, colorless liquid
Purity≥ 95% as analyzed by SDS-PAGE
≥ 90% as analyzed by SEC-HPLC
Concentration by A28010 mg/ml±1 mg/ml
Bioactivity ( in vitro)≥ 90%
Residual DNaseNon-specific DNase activity
Residual RNaseNon-specific RNase activity
Endotoxin Level≤ 10 EU/mg as analyzed by gel clotting method

Examples
  • GenCRISPR™ Ultra ESpCas9-N-NLS Research, Tag-free
    • GenCRISPR™ Ultra ESpCas9-N-NLS Research, Tag-free

    A 20 μl reaction in 1 × Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.